Investigations on the secondary structure of polypeptide chains in polyelectrolyte multilayers and their effect on the adhesion and spreading of osteoblasts.

Abstract:

:Inspired by the composition of the native extracellular matrix, biomimetic polyelectrolyte multilayers were assembled from polypeptides and the glycosaminoglycan chondroitin sulfate (CS). To investigate whether peptide conformation imposes an effect on the cell biological functions of osteoblasts, the secondary structure was analyzed by in situ infra-red and circular dichroism spectroscopy. Multilayers composed of polypeptides and CS reveal a predominantly random coiled conformation and impede osteoblast spreading. On the contrary, polypeptide chains in assemblies of poly-L-lysine and poly-L-glutamic acid (PGA) primarily adopt an intermolecular β sheet structure and reveal an increased area of spread, which consequently supports the proliferation of osteoblasts. When CS is replaced by PGA in mixed multilayers, we observe a structural rearrangement from random coils to β sheets with a concomitant improved cell response. We conclude that polypeptide conformation in biomimetic multilayer assemblies affects osteoblast response by altering the stiffness of the multilayer.

journal_name

Biointerphases

journal_title

Biointerphases

authors

Grohmann S,Rothe H,Liefeith K

doi

10.1007/s13758-012-0062-6

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

62

issue

1-4

eissn

1934-8630

issn

1559-4106

journal_volume

7

pub_type

杂志文章
  • Lateral field excited quartz crystal microbalances for biosensing applications.

    abstract::The most common bulk acoustic wave device used in biosensing applications is the quartz crystal microbalance (QCM), in which a resonant pure shear acoustic wave is excited via electrodes on both major faces of a thin AT-cut quartz plate. For biosensing, the QCM is used to detect the capture of a target by a target-cap...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/6.0000144

    authors: Hartz JSR,Emanetoglu NW,Howell C,Vetelino JF

    更新日期:2020-06-02 00:00:00

  • Native E. coli inner membrane incorporation in solid-supported lipid bilayer membranes.

    abstract::Solid-supported bilayer lipid membranes (SBLMs) containing membrane protein have been generated through a simple lipid dilution technique. SBLM formation from mixtures of native Escherichia coli bacterial inner membrane (IM) vesicles diluted with egg phosphatidylcholine (egg PC) vesicles has been explored with dissipa...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.2896113

    authors: Dodd CE,Johnson BR,Jeuken LJ,Bugg TD,Bushby RJ,Evans SD

    更新日期:2008-06-01 00:00:00

  • Time of flight secondary ion mass spectrometry surface and in-depth study of degradation of nanosheet poly(L-lactic acid) films.

    abstract::With the advent of novel fabrication technologies, free-standing poly(l-lactic acid) (PLLA) nanosheets have been shown to have enhanced performance over their micro- or macroscale equivalents as tissue engineering and drug delivery constructs. In the present research, the authors investigated the surface degradation b...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4908206

    authors: Marchany MD,Gardella JA Jr,Kuchera TJ

    更新日期:2015-03-20 00:00:00

  • Electrochemical switching of the flavoprotein dodecin at gold surfaces modified by flavin-DNA hybrid linkers.

    abstract::Dodecin from Halobacterium salinarum is a dodecameric, hollow-spherical protein, which unspecifically adopts flavin molecules. Reduction of flavin dodecin holocomplexes induces dissociation into apododecin and free flavin. Unspecific binding and dissociation upon reduction were used as key properties to construct an e...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.2965134

    authors: Grininger M,Nöll G,Trawöger S,Sinner EK,Oesterhelt D

    更新日期:2008-09-01 00:00:00

  • Effect of ionic strength on PNA-DNA hybridization on surfaces and in solution.

    abstract::Peptide nucleic acids (PNAs) are mimics of oligonucleotides containing a neutral peptidelike backbone and are able to bind complementary DNA targets with high affinity and selectivity. In order to investigate the effect of the ionic strength of the buffer solution, hybridization experiments with PNAs as (catcher) prob...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.2746871

    authors: Park H,Germini A,Sforza S,Corradini R,Marchelli R,Knoll W

    更新日期:2007-06-01 00:00:00

  • Live cell adhesion assay with attenuated total reflection infrared spectroscopy.

    abstract::Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection (ATR) infrared (IR) spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural r...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.2710336

    authors: Schmidt M,Wolfram T,Rumpler M,Tripp CP,Grunze M

    更新日期:2007-03-01 00:00:00

  • Assembly and structure of alpha-helical peptide films on hydrophobic fluorocarbon surfaces.

    abstract::The structure, orientation, and formation of amphiphilic alpha-helix model peptide films on fluorocarbon surfaces has been monitored with sum frequency generation (SFG) vibrational spectroscopy, near-edge x-ray absorption fine structure (NEXAFS) spectroscopy, and x-ray photoelectron spectroscopy (XPS). The alpha-helix...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.3317116

    authors: Weidner T,Samuel NT,McCrea K,Gamble LJ,Ward RS,Castner DG

    更新日期:2010-03-01 00:00:00

  • Simultaneous measurements of photocurrents and H2O2 evolution from solvent exposed photosystem 2 complexes.

    abstract::In plants, algae, and cyanobacteria, photosystem 2 (PS2) catalyzes the light driven oxidation of water. The main products of this reaction are protons and molecular oxygen. In vitro, however, it was demonstrated that reactive oxygen species like hydrogen peroxide are obtained as partially reduced side products. The tr...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4938090

    authors: Vöpel T,Ning Saw E,Hartmann V,Williams R,Müller F,Schuhmann W,Plumeré N,Nowaczyk M,Ebbinghaus S,Rögner M

    更新日期:2015-03-22 00:00:00

  • Biomimetic coatings enhance tribocorrosion behavior and cell responses of commercially pure titanium surfaces.

    abstract::Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stabi...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4960654

    authors: Marques Ida S,Alfaro MF,Saito MT,da Cruz NC,Takoudis C,Landers R,Mesquita MF,Nociti Junior FH,Mathew MT,Sukotjo C,Barão VA

    更新日期:2016-09-11 00:00:00

  • Nanohybrids consisting of magnetic nanoparticles and gold nanoclusters as effective peroxidase mimics and their application for colorimetric detection of glucose.

    abstract::Although protein-stabilized gold nanoclusters (AuNCs) have gathered recent attention as biocompatible peroxidase mimics, their practical utility has been critically limited by the low catalytic activity. Here, the authors have developed a nanohybrid material to significantly enhance the catalytic activity of AuNCs by ...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4974198

    authors: Cho S,Shin HY,Kim MI

    更新日期:2017-01-17 00:00:00

  • Time-of-flight secondary ion mass spectrometry analyses of vancomycin.

    abstract::As an antibiotic that prevents and treats infections caused by Gram-positive bacteria such as Staphylococcus aureus, vancomycin incorporated in a biodegradable polymer poly(lactide-co-glycolide) provides opportunities to construct controlled-release drug delivery systems. Developments associated with this promising sy...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.5016186

    authors: Du L,Yang X,Li W,Li H,Feng S,Zeng R,Yu B,Xiao L,Liu Y,Tu M,Nie HY

    更新日期:2018-01-10 00:00:00

  • Unraveling the interactions between cold atmospheric plasma and skin-components with vibrational microspectroscopy.

    abstract::Using infrared and Raman microspectroscopy, the authors examined the interaction of cold atmospheric plasma with the skin's built-in protective cushion, the outermost skin layer stratum corneum. Following a spectroscopic analysis, the authors could identify four prominent chemical alterations caused by plasma treatmen...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4919610

    authors: Kartaschew K,Mischo M,Baldus S,Bründermann E,Awakowicz P,Havenith M

    更新日期:2015-06-06 00:00:00

  • X-ray photoelectron spectroscopy characterization of gold nanoparticles functionalized with amine-terminated alkanethiols.

    abstract::Gold nanoparticles (AuNPs) functionalized with a short chain amine-terminated alkanethiol (HS-(CH(2))(2)NH(2) or C2 NH(2)-thiol) are prepared via a direct synthesis method and then ligand-exchanged with a long chain amine-terminated alkanethiol (HS-(CH(2))(11)NH(2) or C11 NH(2)-thiol). Transmission electron microscopy...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.3622481

    authors: Techane SD,Gamble LJ,Castner DG

    更新日期:2011-09-01 00:00:00

  • Immobilized laminin concentration gradients on electrospun fiber scaffolds for controlled neurite outgrowth.

    abstract::Neuronal process growth is guided by extrinsic environmental cues such as extracellular matrix (ECM) proteins. Recent reports have described that the growth cone extension is superior across gradients of the ECM protein laminin compared to growth across uniformly distributed laminin. In this work, the authors have pre...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4857295

    authors: Zander NE,Beebe TP Jr

    更新日期:2014-03-01 00:00:00

  • Combatting fungal biofilm formation by diffusive release of fluconazole from heptylamine plasma polymer coating.

    abstract::A drug-eluting coating applied onto biomedical devices and implants is an appropriate way to ensure that an inhibitory concentration of antimicrobial drugs is present at the device surface, thus preventing surface colonization and subsequent biofilm formation. In this study, a thin polymer coating was applied to mater...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/6.0000511

    authors: Naderi J,Giles C,Saboohi S,Griesser HJ,Coad BR

    更新日期:2020-12-18 00:00:00

  • Astrocyte spreading and migration on aggrecan-laminin dot gradients.

    abstract::The surface concentration gradient of two extracellular matrix (ECM) macromolecules was developed to study the migratory and morphological responses of astrocytes to molecular cues typically found in the central nervous system injury environment. The gradient, prepared using microcontact printing, was composed of rand...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.5001675

    authors: Hsiao TW,Tresco PA,Hlady V

    更新日期:2017-09-11 00:00:00

  • Mixed DNA/oligo(ethylene glycol) functionalized gold surfaces improve DNA hybridization in complex media.

    abstract::Reliable, direct "sample-to-answer" capture of nucleic acid targets from complex media would greatly improve existing capabilities of DNA microarrays and biosensors. This goal has proven elusive for many current nucleic acid detection technologies attempting to produce assay results directly from complex real-world sa...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.2219110

    authors: Lee CY,Gamble LJ,Grainger DW,Castner DG

    更新日期:2006-06-01 00:00:00

  • Coated nanodiamonds interact with tubulin beta-III negative cells of adult brain tissue.

    abstract::Fluorescent nanodiamonds (NDs) coated with therapeutics and cell-targeting structures serve as effective tools for drug delivery. However, NDs circulating in blood can eventually interact with the blood-brain barrier, resulting in undesired pathology. Here, we aimed to detect interaction between NDs and adult brain ti...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/6.0000525

    authors: Šimková V,Freislebenová H,Neuhöferová E,Petráková V,Amini A,Benson V

    更新日期:2020-12-03 00:00:00

  • Vacuum plasma sprayed coatings using ionic silver doped hydroxyapatite powder to prevent bacterial infection of bone implants.

    abstract::Fast and efficient osseointegration of implants into bone is of crucial importance for their clinical success; a process that can be enhanced by coating the implant surface with hydroxyapatite (HA) using the vacuum plasma spray technology (VPS). However, bacterial infections, especially the biofilm formation on implan...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4943225

    authors: Guimond-Lischer S,Ren Q,Braissant O,Gruner P,Wampfler B,Maniura-Weber K

    更新日期:2016-03-10 00:00:00

  • Lysozyme orientation and conformation on MoS2 surface: Insights from molecular simulations.

    abstract::Two-dimensional molybdenum disulfide (MoS2) has attracted intense interest owing to its unique properties and promising biosensor applications. To develop effective biocompatible platforms, it is crucial to understand the interactions between MoS2 and biological molecules such as proteins, but little knowledge exists ...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4984803

    authors: Fan H,Zhao D,Li Y,Zhou J

    更新日期:2017-06-02 00:00:00

  • Antifungal coatings by caspofungin immobilization onto biomaterials surfaces via a plasma polymer interlayer.

    abstract::Not only bacteria but also fungal pathogens, particularly Candida species, can lead to biofilm infections on biomedical devices. By covalent grafting of the antifungal drug caspofungin, which targets the fungal cell wall, onto solid biomaterials, a surface layer can be created that might be able to provide long-term p...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4933108

    authors: Griesser SS,Jasieniak M,Coad BR,Griesser HJ

    更新日期:2015-12-14 00:00:00

  • Observation of endoplasmic reticulum tubules via TOF-SIMS tandem mass spectrometry imaging of transfected cells.

    abstract::Advances in three-dimensional secondary ion mass spectrometry (SIMS) imaging have enabled visualizing the subcellular distributions of various lipid species within individual cells. However, the difficulty of locating organelles using SIMS limits efforts to study their lipid compositions. Here, the authors have assess...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.5019736

    authors: Chini CE,Fisher GL,Johnson B,Tamkun MM,Kraft ML

    更新日期:2018-02-26 00:00:00

  • ToF-SIMS and laser-SNMS analysis of Madin-Darby canine kidney II cells with silver nanoparticles using an argon cluster ion beam.

    abstract::The use of nanoparticles is one of the fastest expanding fields in industrial as well as in medical applications, owing to their remarkable characteristics. Silver nanoparticles (AgNPs) are among the most-commercialized nanoparticles because of their antibacterial effects. Laser postionization secondary neutral mass s...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4937466

    authors: Nees R,Pelster A,Körsgen M,Jungnickel H,Luch A,Galla HJ,Arlinghaus HF

    更新日期:2015-06-15 00:00:00

  • Grafting of architecture controlled poly(styrene sodium sulfonate) onto titanium surfaces using bio-adhesive molecules: Surface characterization and biological properties.

    abstract::This contribution reports on grafting of bioactive polymers such as poly(sodium styrene sulfonate) (polyNaSS) onto titanium (Ti) surfaces. This grafting process uses a modified dopamine as an anchor molecule to link polyNaSS to the Ti surface. The grafting process combines reversible addition-fragmentation chain trans...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4985608

    authors: Chouirfa H,Evans MDM,Castner DG,Bean P,Mercier D,Galtayries A,Falentin-Daudré C,Migonney V

    更新日期:2017-06-14 00:00:00

  • Critical nitric oxide concentration for Pseudomonas aeruginosa biofilm reduction on polyurethane substrates.

    abstract::Bacterial colonies that reside on a surface, known as biofilms, are intrinsically impenetrable to traditional antibiotics, ultimately driving research toward an alternative therapeutic approach. Nitric oxide (NO) has gained attention for its biologically beneficial properties, particularly centered around its antibact...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4962266

    authors: Neufeld BH,Reynolds MM

    更新日期:2016-09-07 00:00:00

  • To be a radical or not to be one? The fate of the stable nitroxide radical TEMPO [(2,2,6,6-Tetramethylpiperidin-1-yl)oxyl] undergoing plasma polymerization into thin-film coatings.

    abstract::The stable nitroxide radical TEMPO [(2,2,6,6-Tetramethylpiperidin-1-yl)oxyl] has a multitude of applications in fields ranging from energy storage to biomedical applications and many more. However, to date, the processes of incorporating nitroxide radicals into thin-film coatings are laborious and not cost-effective, ...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/6.0000259

    authors: Michl TD,Tran DTT,Böttle K,Kuckling HF,Zhalgasbaikyzy A,Ivanovská B,Cavallaro AA,Araque Toledo MA,Sherman PJ,Al-Bataineh SA,Vasilev K

    更新日期:2020-06-26 00:00:00

  • Science and engineering of nanodiamond particle surfaces for biological applications (Review).

    abstract::Diamond has outstanding bulk properties such as super hardness, chemical inertness, biocompatibility, luminescence, to name just a few. In the nanoworld, in order to exploit these outstanding bulk properties, the surfaces of nanodiamond (ND) particles must be accordingly engineered for specific applications. Modificat...

    journal_title:Biointerphases

    pub_type: 杂志文章,评审

    doi:10.1116/1.4927679

    authors: Shenderova OA,McGuire GE

    更新日期:2015-09-05 00:00:00

  • Assessment of protein entrapment in hydroxyapatite scaffolds by size exclusion chromatography.

    abstract::Although it is well known that the textural properties of scaffolds play an important role in the process of tissue regeneration, the investigation of such effects remain difficult especially at the micro/nano level. Texture confers the material the additional ability to entrap/concentrate molecules circulating in the...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1007/s13758-012-0037-7

    authors: Espanol M,Casals I,Lamtahri S,Valderas MT,Ginebra MP

    更新日期:2012-12-01 00:00:00

  • Measuring cell adhesion forces during the cell cycle by force spectroscopy.

    abstract::Force spectroscopy has been used to measure the adhesion of Saos-2 cells to a glass surface at different phases of the cell cycle. The cells were synchronized in three phases of the cell cycle: G(1), S, and G(2)M. Cells in these phases were compared with unsynchronized and native mitotic cells. Individual cells were a...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.3139962

    authors: Weder G,Vörös J,Giazzon M,Matthey N,Heinzelmann H,Liley M

    更新日期:2009-06-01 00:00:00

  • Antifouling coatings for dental implants: Polyethylene glycol-like coatings on titanium by plasma polymerization.

    abstract::Titanium dental implants are commonly used for the replacement of lost teeth, but they present a considerable number of failures due to the infection on surrounding tissues. The aim of this paper is the development of a polyethylene glycol-like (PEG-like) coating on the titanium surface by plasma polymerization to obt...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4913376

    authors: Buxadera-Palomero J,Canal C,Torrent-Camarero S,Garrido B,Javier Gil F,Rodríguez D

    更新日期:2015-06-12 00:00:00