Subpopulations in purified platelets adhering on glass.

Abstract:

:Understanding how platelet activation is regulated is important in the context of cardiovascular disorders and their management with antiplatelet therapy. Recent evidence points to different platelet subpopulations performing different functions. In particular, procoagulant and aggregating subpopulations have been reported in the literature in platelets treated with the GPVI agonists. How the formation of platelet subpopulations upon activation is regulated remains unclear. Here, it is shown that procoagulant and aggregating platelet subpopulations arise spontaneously upon adhesion of purified platelets on clean glass surfaces. Calcium ionophore treatment of the adhering platelets resulted in one platelet population expressing both the procoagulant and the adherent population markers phosphatidylserine and the activated form of GPIIb/IIIa, while all of the platelets expressed CD62P independently of the ionophore treatment. Therefore, all platelets have the capacity to express all three activation markers. It is concluded that platelet subpopulations observed in various studies reflect the dynamics of the platelet activation process.

journal_name

Biointerphases

journal_title

Biointerphases

authors

Donati A,Gupta S,Reviakine I

doi

10.1116/1.4953866

subject

Has Abstract

pub_date

2016-06-22 00:00:00

pages

029811

issue

2

eissn

1934-8630

issn

1559-4106

journal_volume

11

pub_type

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