Abstract:
:In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium plates hydrothermally-treated with CaCl(2) (Ca) or MgCl(2) (Mg) solution, or distilled water (DW). The number and adhesive strengths of attached cells on the substrata were then analyzed. The number of Sa3 cells adhering to the Ca- and Mg-treated plates was significantly larger than in the DW group, but the strength of this adhesion did not differ significantly between groups. In contrast, NIH3T3 cell adhesion number and strength were increased in both the Ca and Mg groups compared to the DW group. Fluorescent microscopic observation indicated that, in all groups, Sa3 had identical expression levels of integrin β4 and development of actin filaments, whereas NIH3T3 cells in the Ca and Mg groups displayed much stronger punctate cytoplasmic signals for vinculin and more bundle-shaped actin filaments than cells in the DW group. As a result, it was indicated that the hydrothermal treatment of titanium with Ca or Mg solution improved the integration of soft tissue cells with the substrata, which may facilitate the development of a soft tissue barrier around the implant.
journal_name
Biointerphasesjournal_title
Biointerphasesauthors
Okawachi H,Ayukawa Y,Atsuta I,Furuhashi A,Sakaguchi M,Yamane K,Koyano Kdoi
10.1007/s13758-012-0027-9subject
Has Abstractpub_date
2012-12-01 00:00:00pages
27issue
1-4eissn
1934-8630issn
1559-4106journal_volume
7pub_type
杂志文章相关文献
Biointerphases文献大全abstract::Understanding and controlling the performance of engineered nanoparticle (NP) systems is greatly assisted by quantitative characterization of their coatings. Useful measurements methods have been described for NPs in liquid environment, but NP aggregation often represents a limiting factor which impairs the accuracy o...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4968882
更新日期:2016-11-30 00:00:00
abstract::Biological membranes and their related molecular mechanisms are essential for all living organisms. Membranes host numerous proteins and are responsible for the exchange of molecules and ions, cell signaling, and cell compartmentation. Indeed, the plasma membrane delimits the intracellular compartment from the extrace...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1116/1.4994299
更新日期:2017-09-28 00:00:00
abstract::The structure, orientation, and formation of amphiphilic alpha-helix model peptide films on fluorocarbon surfaces has been monitored with sum frequency generation (SFG) vibrational spectroscopy, near-edge x-ray absorption fine structure (NEXAFS) spectroscopy, and x-ray photoelectron spectroscopy (XPS). The alpha-helix...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3317116
更新日期:2010-03-01 00:00:00
abstract::The authors investigate membrane composition-mediated interactions between proteins adsorbed onto a two-component lipid bilayer close to critical demixing using coarse-grained molecular dynamics simulations and a phenomenological Ginzburg-Landau theory. The simulations consist of three-bead lipids and platelike protei...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2977492
更新日期:2008-06-01 00:00:00
abstract::The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functions: high resistance to nonspecific protein adsorption, efficient surface anchoring, and subsequent covalent a...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2801974
更新日期:2007-12-01 00:00:00
abstract::Shewanella oneidensis MR-1 is a dissimilatory metal-reducing bacterium capable of reducing various metal and sulfur compounds and precipitating them in nanoparticulate form. Here, we report the synthesis of molybdenum disulfide nanomaterials at the site of S. oneidensis biofilms grown in the presence of molybdenum tri...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000199
更新日期:2020-07-24 00:00:00
abstract::Advances in three-dimensional secondary ion mass spectrometry (SIMS) imaging have enabled visualizing the subcellular distributions of various lipid species within individual cells. However, the difficulty of locating organelles using SIMS limits efforts to study their lipid compositions. Here, the authors have assess...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5019736
更新日期:2018-02-26 00:00:00
abstract::Solid-supported bilayer lipid membranes (SBLMs) containing membrane protein have been generated through a simple lipid dilution technique. SBLM formation from mixtures of native Escherichia coli bacterial inner membrane (IM) vesicles diluted with egg phosphatidylcholine (egg PC) vesicles has been explored with dissipa...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2896113
更新日期:2008-06-01 00:00:00
abstract::Nanodiamond (ND) particles are popular platforms for the immobilization of molecular species. In the present research, enzyme Escherichia coli inorganic pyrophosphatase (PPase) was immobilized on detonation ND through covalent or noncovalent bonding and its enzymatic activity was characterized. Factors affecting adsor...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4934483
更新日期:2015-12-21 00:00:00
abstract::It has been demonstrated that electric fields can minimize surface adhesion of bacteria, but how they affect the near-surface bacterial dynamics has remained largely overlooked. In the present study, the three-dimensional motions of Escherichia coli (E. coli) HCB1 and HCB1414 near a conductive surface under alternatin...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5078543
更新日期:2019-02-20 00:00:00
abstract::Fast and efficient osseointegration of implants into bone is of crucial importance for their clinical success; a process that can be enhanced by coating the implant surface with hydroxyapatite (HA) using the vacuum plasma spray technology (VPS). However, bacterial infections, especially the biofilm formation on implan...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4943225
更新日期:2016-03-10 00:00:00
abstract::Photocorrosion of semiconductors is strongly sensitive to the presence of surface states, and it could be influenced by electrically charged molecules immobilized near the semiconductor/electrolyte interface. The underlying mechanism is related to band bending of the semiconductor structure near the surface and the as...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4941983
更新日期:2016-03-22 00:00:00
abstract::Mucus is a viscous slime that plays a vital role in protecting and lubricating biological tissues, in particular, soft epithelium interfaces such as in the stomach, intestines, and esophagus. Previous attempts to generate mucus models that mimick or simulate its characteristics have been predominantly focused on the r...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003708
更新日期:2017-12-05 00:00:00
abstract::Peptide nucleic acids (PNAs) are mimics of oligonucleotides containing a neutral peptidelike backbone and are able to bind complementary DNA targets with high affinity and selectivity. In order to investigate the effect of the ionic strength of the buffer solution, hybridization experiments with PNAs as (catcher) prob...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2746871
更新日期:2007-06-01 00:00:00
abstract::Diamond has outstanding bulk properties such as super hardness, chemical inertness, biocompatibility, luminescence, to name just a few. In the nanoworld, in order to exploit these outstanding bulk properties, the surfaces of nanodiamond (ND) particles must be accordingly engineered for specific applications. Modificat...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1116/1.4927679
更新日期:2015-09-05 00:00:00
abstract::Permanent implants made from titanium are widely used and successfully implemented in medicine to address problems related to orthopedic and oral disorders. However, implants that interact in all cases optimally and durably with bone tissue have yet to be developed. Here, the authors suggest a phospholipids 1-palmitoy...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4938556
更新日期:2015-03-29 00:00:00
abstract::The growing interest in artificial bioorganic interfaces as a platform for applications in emerging areas as personalized medicine, clinical diagnostics, biosensing, biofilms, prevention of biofouling, and other fields of bioengineering is the origin of a need for in detail multitechnique characterizations of such lay...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4948341
更新日期:2016-06-02 00:00:00
abstract::In plants, algae, and cyanobacteria, photosystem 2 (PS2) catalyzes the light driven oxidation of water. The main products of this reaction are protons and molecular oxygen. In vitro, however, it was demonstrated that reactive oxygen species like hydrogen peroxide are obtained as partially reduced side products. The tr...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4938090
更新日期:2015-03-22 00:00:00
abstract::Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stabi...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4960654
更新日期:2016-09-11 00:00:00
abstract::The kinetics of adsorption and desorption of two highly asymmetrical model peptides were studied at methyl- and carboxylic acid-terminated alkylthiolate self-assembled monolayer (SAM) surfaces on gold. The model peptides were leucine-lysine (LK), α-helical (LKα14), and β-strand (LKβ15) peptides that have a well-define...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3494080
更新日期:2010-12-01 00:00:00
abstract::A drug-eluting coating applied onto biomedical devices and implants is an appropriate way to ensure that an inhibitory concentration of antimicrobial drugs is present at the device surface, thus preventing surface colonization and subsequent biofilm formation. In this study, a thin polymer coating was applied to mater...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000511
更新日期:2020-12-18 00:00:00
abstract::The authors report on the characterization of the charge formation at supported bilayer lipid membranes (sBLMs) prepared from the zwitterionic lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine on planar silicon dioxide substrates. The charging of the sBLMs was studied in KCl solutions of different ionic strengths be...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3082042
更新日期:2009-01-01 00:00:00
abstract::In this study, the authors report that sodium citrate can aggregate hexadecyl-trimethyl-ammonium ion(+)-coated gold nanorods (AuNRs), and nucleic acids of different charge and structure properties, i.e., single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), single-stranded peptide nucleic acid (PNA), and PNA-DNA c...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3496962
更新日期:2010-09-01 00:00:00
abstract::Unmodified gelatin (uG) is widely used as a coating material in cell culture for improving surface properties. In this study, the authors investigated if gelatin methacrylamide (GM) with a medium degree of methacrylamide modification (GM1.5) and a high degree of methacrylamide modification (GM4) are equally suitable f...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4949545
更新日期:2016-06-13 00:00:00
abstract::Inhalation of combustion-derived ultrafine particles (≤0.1 μm) has been found to be associated with pulmonary and cardiovascular diseases. However, correlation of the physicochemical properties of carbon-based particles such as surface charge and agglomeration state with adverse health effects has not yet been establi...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5043373
更新日期:2018-09-11 00:00:00
abstract::With the advent of novel fabrication technologies, free-standing poly(l-lactic acid) (PLLA) nanosheets have been shown to have enhanced performance over their micro- or macroscale equivalents as tissue engineering and drug delivery constructs. In the present research, the authors investigated the surface degradation b...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4908206
更新日期:2015-03-20 00:00:00
abstract::The precise use of nanoparticles in technological applications requires control over their surface properties. This implies the ability to quantitatively describe, for example, molecular coatings in terms of their thickness, areal mass, or number of molecules. Here, the authors describe two different approaches to the...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4913566
更新日期:2015-03-27 00:00:00
abstract::The most common bulk acoustic wave device used in biosensing applications is the quartz crystal microbalance (QCM), in which a resonant pure shear acoustic wave is excited via electrodes on both major faces of a thin AT-cut quartz plate. For biosensing, the QCM is used to detect the capture of a target by a target-cap...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000144
更新日期:2020-06-02 00:00:00
abstract::The aim was to compare the protein release from normal human osteoblasts (NHO) cultured on scaffolds with similar morphology but different coatings. Different ceramic coatings; TiO(2), SiO(2) and calcium phosphate (CaP); Ca(9)HPO(4)(PO(4))(5)OH, were applied to porous TiO(2) scaffolds prepared by polymer sponge replic...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-012-0036-8
更新日期:2012-12-01 00:00:00
abstract::Magnesium alloys are potential biodegradable implants for biomedical applications, and calcium (Ca) is one kind of ideal element being examined for magnesium alloys and biodegradable ceramic coatings owing to its biocompatibility and mechanical suitability. In this study, microarc oxidation (MAO) coatings were prepare...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003320
更新日期:2018-01-16 00:00:00