Assessment of protein entrapment in hydroxyapatite scaffolds by size exclusion chromatography.

Abstract:

:Although it is well known that the textural properties of scaffolds play an important role in the process of tissue regeneration, the investigation of such effects remain difficult especially at the micro/nano level. Texture confers the material the additional ability to entrap/concentrate molecules circulating in the body fluid regardless of their binding affinity to the material. The goal of the present work is to isolate protein entrapment from protein adsorption phenomena in two macroporous hydroxyapatite scaffolds with identical chemical structure, similar macroporosity but different micro/nanoporosity using proteins of different sizes. This was achieved implementing size exclusion chromatography and using the scaffolds as chromatographic columns. The results showed that the larger the crystal size and the lower the packing density of the crystals composing the scaffold increased protein retention but decreased the protein dwelling time in the column. Differences in the amount of protein retained depended on the protein type.

journal_name

Biointerphases

journal_title

Biointerphases

authors

Espanol M,Casals I,Lamtahri S,Valderas MT,Ginebra MP

doi

10.1007/s13758-012-0037-7

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

37

issue

1-4

eissn

1934-8630

issn

1559-4106

journal_volume

7

pub_type

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