Abstract：
:Like many fields of the biosciences, actinomycete natural products research has been revolutionised by next-generation DNA sequencing (NGS). Hundreds of new genome sequences from actinobacteria are made public every year, many of them as a result of projects aimed at identifying new natural products and their biosynthetic pathways through genome mining. Advances in these technologies in the last five years have meant not only a reduction in the cost of whole genome sequencing, but also a substantial increase in the quality of the data, having moved from obtaining a draft genome sequence comprised of several hundred short contigs, sometimes of doubtful reliability, to the possibility of obtaining an almost complete and accurate chromosome sequence in a single contig, allowing a detailed study of gene clusters and the design of strategies for refactoring and full gene cluster synthesis. The impact that these technologies are having in the discovery and study of natural products from actinobacteria, including those from the marine environment, is only starting to be realised. In this review we provide a historical perspective of the field, analyse the strengths and limitations of the most relevant technologies, and share the insights acquired during our genome mining projects.
journal_name
Mar Drugsjournal_title
Marine drugsauthors
Gomez-Escribano JP,Alt S,Bibb MJdoi
10.3390/md14040078subject
Has Abstractpub_date
2016-04-13 00:00:00issue
4issn
1660-3397pii
md14040078journal_volume
14pub_type
杂志文章,评审相关文献
Marine Drugs文献大全abstract：:The vast oceans of the world, which comprise a huge variety of unique ecosystems, are emerging as a rich and relatively untapped source of novel bioactive compounds with invaluable biotechnological and pharmaceutical potential. Evidence accumulated over the last decade has revealed that the diversity of marine microor...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md13052924
更新日期：2015-05-13 00:00:00
abstract：:Three new benzofuranoids, asperfuranoids A-C (1-3), two new phenylpropanoid derivatives (6 and 7), and nine known analogues (4, 5, and 8-14) were isolated from the liquid substrate fermentation cultures of the mangrove endopytic fungus Aspergillus sp. ZJ-68. The structures of the new compounds were determined by exten...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md17080478
更新日期：2019-08-18 00:00:00
abstract：:Acute myeloid leukemia (AML) is a heterogeneous myeloid clonal disorder exhibiting the accumulation of immature myeloid progenitors in the bone marrow and peripheral blood. Standard AML therapy requires intensive combination chemotherapy, which leads to significant treatment-related toxicity. The search for new, low t...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md14110205
更新日期：2016-11-03 00:00:00
abstract：:In this study, Spirulina maxima derived pectin nanoparticles (SmPNPs) were synthesized and multiple biological effects were investigated using in vitro and in vivo models. SmPNPs were not toxic to Raw 264.7 cells and zebrafish embryos up to 1 mg/mL and 200 µg/mL, respectively. SmPNPs upregulated Il 10, Cat, Sod 2, Def...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md18110556
更新日期：2020-11-07 00:00:00
abstract：:Tisochrysis lutea is a marine haptophyte rich in omega-3 polyunsaturated fatty acids (e.g., docosahexaenoic acid (DHA)) and carotenoids (e.g., fucoxanthin). Because of the nutraceutical applications of these compounds, this microalga is being used in aquaculture to feed oyster and shrimp larvae. In our earlier report,...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md18110567
更新日期：2020-11-19 00:00:00
abstract：:Two new piperazine-triones lansai E and F (1, 2), together with four known secondary metabolites lansai D (3), 1-N-methyl-(E,Z)-albonoursin (4), imidazo[4,5-e]-1,2,4-triazine (5), and streptonigrin (6) were isolated from a deep-sea-derived Streptomycetes sp. strain SMS636. The structures of the isolated compounds were...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md17030186
更新日期：2019-03-21 00:00:00
abstract：:Marine natural products are a source of promising agents for cancer treatment. However, there is a need to improve the evaluation of their mechanism of action in tumors. Metabolomics of the response to anti-tumor agents is a tool to reveal candidate biomarkers and metabolic targets. We used two-dimensional high-resolu...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md11103846
更新日期：2013-10-11 00:00:00
abstract：:The predatory marine gastropod Dicathais orbita has been the subject of a significant amount of biological and chemical research over the past five decades. Natural products research on D. orbita includes the isolation and identification of brominated indoles and choline esters as precursors of Tyrian purple, as well ...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md11041370
更新日期：2013-04-23 00:00:00
abstract：:Carotenoids are lipid-soluble pigments that are produced in some plants, algae, fungi, and bacterial species, which accounts for their orange and yellow hues. Carotenoids are powerful antioxidants thanks to their ability to quench singlet oxygen, to be oxidized, to be isomerized, and to scavenge free radicals, which p...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md13106226
更新日期：2015-09-30 00:00:00
abstract：:In our current investigation, we evaluated the effect of Chlorella pyrenoidosa protein hydrolysate (CPPH) and Chlorella pyrenoidosa protein hydrolysate-calcium chelate (CPPH-Ca) on calcium absorption and gut microbiota composition, as well as their in vivo regulatory mechanism in SD rats fed low-calcium diets. Potent ...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md17060348
更新日期：2019-06-11 00:00:00
abstract：:Peridinin and fucoxanthin, which are natural carotenoids isolated from a symbiotic dinoflagellate, Symbiodinium sp., and a brown alga, Petalonia fascia, respectively, were compared for inhibitory effects on delayed-type hypersensitivity in mice. The number of eosinophils at the site of inflammation and in peripheral b...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md12041773
更新日期：2014-03-27 00:00:00
abstract：:The swim bladder of the croceine croaker (Pseudosciaena crocea) was believed to have good curative effects in various diseases, including amnesia, insomnia, dizziness, anepithymia, and weakness after giving birth, in traditional Chinese medicine. However, there is no research focusing on the antioxidant and anti-fatig...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md14120221
更新日期：2016-12-13 00:00:00
abstract：:Ecklonia cava (E. cava) can alleviate vascular dysfunction in diseases associated with poor circulation. E. cava contains various polyphenols with different functions, but few studies have compared the effects of these polyphenols. Here, we comparatively investigated four major compounds present in an ethanoic extract...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md16110441
更新日期：2018-11-09 00:00:00
abstract：:The purpose of this study was to develop a pharmaceutical formulation containing fatty acid extract rich in free omega-3 fatty acids such as eicosapentaenoic acid and docosahexaenoic acid for topical use. Although the health benefits of cod liver oil and other fish oils taken orally as a dietary supplement have been a...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md14060105
更新日期：2016-06-01 00:00:00
abstract：:During an investigation of the chemistry of the Red Sea Verongiid sponge Pseudoceratina arabica, we discovered a small molecule, pseudoceratonic acid (1), along with the new moloka'iamine derivatives, ceratinines N (2), O (3), and the previously reported compounds moloka'iamine (4), hydroxymoloka'iamine (5) and cerati...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md18110525
更新日期：2020-10-23 00:00:00
abstract：:The marine genus Pseudoalteromonas is known for its versatile biotechnological potential with respect to the production of antimicrobials and enzymes of industrial interest. We have sequenced the genomes of three Pseudoalteromonas sp. strains isolated from different deep sea sponges on the Illumina MiSeq platform. The...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15060184
更新日期：2017-06-19 00:00:00
abstract：:24-methyl-cholesta-5,24(28)-diene-3β,19-diol-7β-monoacetate (MeCDDA) is a natural steroid compound isolated from a wild-type soft coral (Nephthea erecta). The present study aimed to investigate the anti-small cell lung cancer (SCLC) effects of MeCDDA in vitro and in vivo, as well as to elucidate its underlying mechani...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15070210
更新日期：2017-07-01 00:00:00
abstract：:The marine environment is host to unparalleled biological and chemical diversity, making it an attractive resource for the discovery of new therapeutics for a plethora of diseases. Compounds that are extracted from cyanobacteria are of special interest due to their unique structural scaffolds and capacity to produce p...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md14010017
更新日期：2016-01-12 00:00:00
abstract：:Several known sesquiterpenoid quinones and quinols (1-9), and kauamide (10), a new polyketide-peptide containing an 11-membered heterocycle, were isolated from the extracts of the Hawaiian marine sponge Dactylospongiaelegans. The planar structure of 10 was determined from spectroscopic analyses, and its relative and a...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md17070423
更新日期：2019-07-19 00:00:00
abstract：:Carotenoids are lipophilic pigments found in plants and algae, as well as some bacteria, archaea, and fungi that serve two functions-(1) as light harvesting molecules-primary carotenoids, and (2) as antioxidants, acting against reactive oxygen species⁻secondary carotenoids. Because of their strong antioxidant properti...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md17030187
更新日期：2019-03-22 00:00:00
abstract：:Ascidians (tunicates) are invertebrate chordates, and prolific producers of a wide variety of biologically active secondary metabolites from cyclic peptides to aromatic alkaloids. Several of these compounds have properties which make them candidates for potential new drugs to treat diseases such as cancer. Many of the...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md16050162
更新日期：2018-05-13 00:00:00
abstract：:Natural marine products have shown an interesting array of diverse and novel chemical structures with potent biological activities. Our study reports the antiproliferative assays of crude extracts, fraction and pure compound (4R,9S,14S)-4α-acetoxy-9β,14α-dihydroxydolast-1(15),7-diene (1) obtained from brown alga Canis...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md9112369
更新日期：2011-01-01 00:00:00
abstract：:Diabetes mellitus is a group of metabolic disorders of the endocrine system characterised by hyperglycaemia. Type II diabetes mellitus (T2DM) constitutes the majority of diabetes cases around the world and are due to unhealthy diet, sedentary lifestyle, as well as rise of obesity in the population, which warrants the ...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md13085447
更新日期：2015-08-21 00:00:00
abstract：:Species in the genus Gambierdiscus produce ciguatoxins (CTXs) and/or maitotoxins (MTXs), which may cause ciguatera fish poisoning (CFP) in humans if contaminated fish are consumed. Species of Gambierdiscus have previously been isolated from macroalgae at Rangitahua (Raoul Island and North Meyer Islands, northern Kerma...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15070219
更新日期：2017-07-11 00:00:00
abstract：:A new eunicellin diterpenoid, cladieunicellin I (1), and a new natural eunicellin, litophynin I diacetate (2), were isolated from a Formosan soft coral identified as Cladiella sp. The structures of eunicellins 1 and 2 were elucidated by spectroscopic methods and by comparison of the spectral data with those of related...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md11114585
更新日期：2013-11-14 00:00:00
abstract：:Padina sanctae-crucis Børgesen is distributed worldwide in tropical and subtropical seas; belongs to the Dictyotaceae family, and has proven to be an exceptional source of biologically active compounds. Four compounds were isolated and identified, namely: dolastane diterpene new for the genus Padina; phaeophytin and h...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15100251
更新日期：2017-09-26 00:00:00
abstract：:Biologically active compounds with different modes of action, such as, antiproliferative, antioxidant, antimicrotubule, have been isolated from marine sources, specifically algae and cyanobacteria. Recently research has been focused on peptides from marine animal sources, since they have been found as secondary metabo...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md10050963
更新日期：2012-05-01 00:00:00
abstract：:Two new steroids, crellasterones A (1) and B (2), together with the previously reported compound chalinasterol (3) and several nucleosides (4-7), were isolated from the sponge Crella incrustans, collected in New Caledonia. The structures of the new compounds were established by extensive NMR and mass spectroscopic ana...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15060177
更新日期：2017-06-15 00:00:00
abstract：:We performed a high-content screening (HCS) assay aiming to discover bioactive molecules with proteasome inhibitory activity. By structural elucidation, we identified six compounds purified from soft coral Clavularia flava, which potentiates proteasome inhibition. Chemical structure elucidation revealed they are dolab...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md18010039
更新日期：2020-01-03 00:00:00
abstract：:Application of cytostatics in cancer patients' chemotherapy results in a number of side effects, including the inhibition of various parts of hematopoiesis. Two sulfated polysaccharides, fucoidan from the seaweed Chordaria flagelliformis (PS-Fuc) and fucosylated chondroitin sulfate from the sea cucumber Massinium magn...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15100301
更新日期：2017-09-30 00:00:00
