Abstract:
:Erythropoietin (EPO), a hematopoietic hormonal cytokine induced in response to hypoxia, has neuroprotective effects. EPO receptor (EPOR) is expressed in microglia, resident immune cells in the brain. However, the effect of EPO on microglial activation is not clear. In the present study, we demonstrated that the EPOR is highly expressed in microglia, rather than in neurons or astrocytes, in in vitro experiments. Therefore, we investigated whether EPO could attenuate lipopolysaccharide (LPS)-mediated activation of microglia in vitro. The BV-2 microglial cell line was treated with LPS in the absence or presence of EPO. In the presence of EPO, microglial expression of LPS-induced inflammatory cytokine genes was significantly decreased. In addition, EPO suppressed the LPS-induced phagocytic activity of BV-2 cells towards fluorescent beads, as well as induction of inducible nitric oxide synthase. In in vivo experiments, EPO significantly decreased the LPS-induced expression of inflammatory cytokine genes in mouse brains. Furthermore, morphological analysis of cortical microglia in the brains of mice stimulated with LPS revealed that combined treatment with EPO alleviated LPS-induced morphological changes in the microglia. These data indicate that EPO attenuates microglial activation, including morphological changes in vivo, phagocytosis in vitro, and the production of inflammatory cytokines in vivo and in vitro. Further investigation of EPO modulation of LPS-induced microglial activation may contribute to the development of novel neuroprotective therapies.
journal_name
Brain Resjournal_title
Brain researchauthors
Tamura T,Aoyama M,Ukai S,Kakita H,Sobue K,Asai Kdoi
10.1016/j.brainres.2017.02.023subject
Has Abstractpub_date
2017-05-01 00:00:00pages
65-74eissn
0006-8993issn
1872-6240pii
S0006-8993(17)30093-8journal_volume
1662pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::The role of histamine in mediating restraint stress-induced alterations in dopaminergic neuronal activity and alpha-melanocyte-stimulating hormone (alpha MSH) secretion was evaluated in male rats. Dopaminergic neuronal activity was estimated by measuring concentrations of the dopamine metabolite 3,4-dihydroxyphenylace...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90399-9
更新日期:1994-08-08 00:00:00
abstract::Pretreatment with recombinant human interleukin-1 alpha (rhIL-1 alpha) 20 h before irradiation attenuates radiation-induced hyperthermia. Experiments were conducted to determine the role of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) in rhIL-1 alpha-induced attenuation of ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91576-e
更新日期:1993-03-19 00:00:00
abstract::We employed a highly sensitive combination method of retrograde tracing and immunohistochemistry to identify calcitonin gene-related peptide (CGRP)-containing fiber pathways in the rat from the ventrolateral part of the ventrolateral thalamic nucleus (vl-vl) and the caudal continuation to the insular cortex. Biotin-wh...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91211-9
更新日期:1985-09-30 00:00:00
abstract::A startle-like response can be evoked at low currents by one-pulse electrical stimulation of reticular formation sites from the rostrolateral pons to the caudomedial medulla. To test whether this response is mediated by the same reticular formation neurons as those that mediate the acoustic startle, we delivered a bri...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91100-7
更新日期:1993-07-23 00:00:00
abstract::This experiment was conducted to determine if nigrostriatal dopamine (DA) neurons are necessary for the contraversive rotational behavior evoked by electrical stimulation in the lateral hypothalamus. Rats were tested daily for electrical stimulation-induced rotational behavior (ESRB) for 5 days, and then given an inje...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91508-2
更新日期:1985-02-18 00:00:00
abstract::Cholecystokinin octapeptide (CCK-8) is colocalized within a majority of dopamine (DA)-containing neurons of the rat midbrain. Exogenous CCK-8 can modulate the electrophysiological activity of DA neurons, at least in part, by direct actions on the somatodendritic region of these cells. If CCK-8 is somatodendritically r...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90353-w
更新日期:1991-08-02 00:00:00
abstract::While exercise is commonly recommended for PD patients to improve motor function, little is known about the disease-altering potential of exercise. Although others have demonstrated neuroprotective or neurorestorative effects of exercise in animal models of PD, the majority of these studies utilize young animals. In o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2016.06.032
更新日期:2016-09-01 00:00:00
abstract::The immunocytochemical localization of L-glutamate decarboxylase (GAD), the enzyme which which forms gamma-aminobutyric acid (GABA), has been studied in developing rodent cerebellum. During the first 3-4 postnatal days, GAD is distributed along non-terminal portions of axonal processes in close association with small ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(75)90813-6
更新日期:1975-03-07 00:00:00
abstract::The role of central delta-opioid receptors in the mediation of opioid reinforcement and endogenous reward processes was examined using a non-biased place-preference conditioning procedure. Intracerebroventricular (i.c.v.) administration of the selective delta-receptor agonist, [D-Pen2, D-Pen5]-enkephalin (DPDPE, 10.0-...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91667-2
更新日期:1987-12-15 00:00:00
abstract::We investigated the protective effect of subchronic treatment of the A2A receptor antagonist, SCH 58261 (0.01 mg/kg, i.p.), administered 5 min, 6 h and 15 h after permanent right middle cerebral artery occlusion (MCAo). Twenty-four hours after ischemia, an extensive pallid area, evaluated by cresyl violet staining, is...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.12.010
更新日期:2006-02-16 00:00:00
abstract::The expression of two embryonic intermediate filaments, nestin and vimentin, in the rat brain at days 0 (control), 1, 4, 7 and 14 post-cryoinjury was studied to elucidate their roles in brain injury. Western blot analysis showed that both nestin and vimentin expressions in the ipsilateral cerebral cortex were signific...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.09.022
更新日期:2004-12-03 00:00:00
abstract::The effect of an extended program of perforant path or amygdala kindling on paired-pulse suppression in the dentate gyrus was studied in male hooded rats. Repeated kindling stimulations were delivered twice or three times daily until either 300 stimuli had been delivered or generalized convulsions had been observed to...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)01277-o
更新日期:1995-01-23 00:00:00
abstract::An object recognition task was used to determine if chronic restraint stress (6 h/day for 21 days) impairs non-spatial memory, since chronic restraint is known to impair spatial memory. In addition, food deprivation was tested as a possible modulating factor of any stress effect in this non-reward-dependent task. Foll...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01380-3
更新日期:1999-05-29 00:00:00
abstract::Eleven beta-carbolinium compounds (beta C+s) and MPP+ were stereotaxically injected (40-200 nmol in 5 microliter of vehicle) unilaterally into the substantia nigra of anesthetized adult male Sprague-Dawley rats. The rats were sacrificed after three weeks. The ipsilateral striatum was analyzed for dopamine and DOPAC le...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00082-2
更新日期:1995-03-27 00:00:00
abstract::The binding characteristics of [3H]cytisine, a putative CNS nicotinic receptor ligand, were examined in 4 regions of the human brain. [3H]Cytisine was found to bind non-cooperatively with high affinity to a single site in tissue homogenates and to exhibit low non-specific association. The binding characteristics of th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90410-o
更新日期:1993-01-08 00:00:00
abstract::Hippocampal cholinergic neurons are sensitive to acute ethanol administration, and specific alterations are seen in the functioning of these neurons following chronic ethanol. The present study examined the effects of chronic ethanol exposure and withdrawal on the sensitivity of the hippocampus to local injection of p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90500-1
更新日期:1985-06-03 00:00:00
abstract::Neurointermediate lobes of rats comprise elements which, when excited in vitro, bring about an inhibition of the release of melanocyte stimulating hormone (MSH). Superfusion of neurointermediate lobes of intact donor rats with medium containing 45 mM K+ induced a stimulation of the release of oxytocin, arginine-vasopr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)91047-3
更新日期:1979-08-10 00:00:00
abstract::This study was conducted on unanesthetized rainbow trout equipped with two ECG electrodes and with an intracerebroventricular (i.c.v.) micro-guide. The ECG signal was recorded during three experimental sessions of 30 min and the heart rate variability (HRV) spectral analysis was performed during stabilized periods of ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)02903-7
更新日期:2002-08-23 00:00:00
abstract::We investigated the effect of focal cerebral ischaemia on the activity and the affinity of the ouabain sites of Na+,K+-ATPase in the mouse. The Na+,K+-ATPase activity was decreased by 38% as early as 30 min following ischaemia. In the sham group, the dose-response curves for ouabain disclosed three inhibitory states w...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)81695-2
更新日期:1997-11-07 00:00:00
abstract::While trophic support from targets depends on innervation, recent evidence suggests that local VIP promotes survival of sympathetic neuroblasts prior to target interactions, during the period of neurogenesis. Developmental studies now indicate that VIP expression peaks at embryonic day 15.5 (E15.5) in sympathetic gang...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90461-8
更新日期:1994-11-07 00:00:00
abstract::We investigated the effect of adenosine A1 receptors on the release of acetylcholine (ACh) and GABA, and on the intracellular calcium concentration ([Ca2+]i) response in cultured chick amacrine-like neurons, stimulated by KCl depolarization. The KCl-induced release of [3H]ACh, but not the release of [14C]GABA, was pot...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02155-1
更新日期:2000-01-03 00:00:00
abstract::This study investigated the effects of antidromically conducted nerve impulses on the transmission of orthodromic volleys in primary afferents of the hindlimb in decerebrated paralyzed cats. Two protocols were used: (A) Single skin and muscle afferents (N=20) isolated from the distal part of cut dorsal rootlets (L7-S1...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01236-6
更新日期:1999-04-17 00:00:00
abstract::Collagen is one of the best materials used for nerve guide preparation due to its biocompatibility and desirable tensile strength. In this work, we have compared regeneration and functional reinnervation after sciatic nerve resection with bioresorbable crosslinked collagen guides in 10 mm gap. The crosslinking was car...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.03.022
更新日期:2005-06-07 00:00:00
abstract::The coupling of muscarinic-cholinergic receptors (mAChR) to adenylate cyclase and phospholipase C (PLC) second messenger systems has been demonstrated in many animal species. However, little is known about this association in the developing human central nervous system. Because of the proposed role of acetylcholine in...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90365-4
更新日期:1994-08-08 00:00:00
abstract::To determine whether oculomotor internuclear neurons (INOs) establish unilateral or bilateral connections with the abducens nucleus (nVI), horseradish peroxidase (HRP) and either DAPI or Nuclear Yellow (NY) were injected into nVI and the oculomotor complex (nIII) examined for retrogradely labeled cells. Such injection...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90464-x
更新日期:1983-02-28 00:00:00
abstract::The role of thyrotropin releasing hormone (TRH) in the dorsal vagal complex (DVC) in mediating the enhanced gastric contractility induced by glutamate (100 pmol) microinjected into the raphe pallidus (Rpa) was investigated in urethane-anesthetized rats acutely implanted with miniature strain gauge force transducers on...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91035-9
更新日期:1994-02-14 00:00:00
abstract::Following the injection of small volumes of horseradish peroxidase conjugated with wheatgerm agglutinin into the substantia nigra of the rat, a high proportion of the total neuronal population in parts of the ipsilateral neostriatum was retrogradely labelled with horseradish peroxidase. Quantitative estimates in 1-mic...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)91222-1
更新日期:1981-09-14 00:00:00
abstract::Murine cortical cell cultures deprived of glucose for 6-8 h developed extensive neuronal degeneration, apparent both morphologically and by efflux of lactate dehydrogenase to the bathing medium. This neuronal damage could be substantially reduced by addition of D-2-amino-5-phosphonovalerate (D-APV), in a concentration...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90179-0
更新日期:1989-04-03 00:00:00
abstract::The firing patterns of up to 4 concurrently active masseter motor units were studied with intramuscular electrodes during a continuous isometric contraction of 15 min duration, in which the subject maintained the mean firing rate of one selected unit at 10 Hz. With this paradigm the net excitation (i.e. mean firing ra...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90467-a
更新日期:1991-05-24 00:00:00
abstract::The metabolic effects of intravenous administration of 200 mg/kg imidazole-4-acetic acid (IMA) on normoxic, hypoxemic and hypoxemic-oligemic rat brain were assessed by measurement of the cerebral contents of energy phosphates and selected glycolytic-citric acid cycle intermediates. In normoxic brain IMA was associated...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90286-4
更新日期:1982-01-28 00:00:00