Abstract:
:Compared to conventional techniques, Laser surgery procedures provide a number of advantages, but may be associated with an increased risk of iatrogenic damage to important anatomical structures. The type of tissue ablated in the focus spot is unknown. Laser-Induced Breakdown-Spectroscopy (LIBS) has the potential to gain information about the type of material that is being ablated by the laser beam. This may form the basis for tissue selective laser surgery. In the present study, 7 different porcine tissues (cortical and cancellous bone, nerve, mucosa, enamel, dentine and pulp) from 6 animals were analyzed for their qualitative and semiquantitative molecular composition using LIBS. The so gathered data was used to first differentiate between the soft- and hard-tissues using a Calcium-Carbon emission based classifier. The tissues were then further classified using emission-ratio based analysis, principal component analysis (PCA) and linear discriminant analysis (LDA). The relatively higher concentration of Calcium in the hard tissues allows for an accurate first differentiation of soft- and hard tissues (100% sensitivity and specificity). The ratio based statistical differentiation approach yields results in the range from 65% (enamel-dentine pair) to 100% (nerve-pulp, cancellous bone-dentine, cancellous bone-enamel pairs) sensitivity and specificity. Experimental LIBS measuring setup.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Rohde M,Mehari F,Klämpfl F,Adler W,Neukam FW,Schmidt M,Stelzle Fdoi
10.1002/jbio.201600153subject
Has Abstractpub_date
2017-10-01 00:00:00pages
1250-1261issue
10eissn
1864-063Xissn
1864-0648journal_volume
10pub_type
杂志文章abstract::In fluctuation-based optical nanoscopy, investigating high-density labeled subcellular structures with high fidelity has been a significant challenge. In this study, based on super-resolution radial fluctuation (SRRF) microscopy, the joint tagging (JT) strategy is employed to enable fast high-density nanoscopic imagin...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800020
更新日期:2018-09-01 00:00:00
abstract::Optical coherence tomography (OCT) imaging shows a significant potential in clinical routines due to its noninvasive property. However, the quality of OCT images is generally limited by inherent speckle noise of OCT imaging and low sampling rate. To obtain high signal-to-noise ratio (SNR) and high-resolution (HR) OCT ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000282
更新日期:2021-01-01 00:00:00
abstract::Intravascular optical coherence tomography (IV-OCT) is a light-based imaging modality with high resolution, which employs near-infrared light to provide tomographic intracoronary images. Morbidity caused by coronary heart disease is a substantial cause of acute coronary syndrome and sudden cardiac death. The most comm...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900112
更新日期:2020-01-01 00:00:00
abstract::Photoacoustic imaging (PAI) is a hybrid imaging modality with high resolution and sensitivity that can be beneficial for cancer staging. Due to insufficient endogenous photoacoustic (PA) contrast, the development of exogenous agents is critical in targeting cancerous tumors. The current study demonstrates the feasibil...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600159
更新日期:2017-08-01 00:00:00
abstract::Photobiomodulation (PBM) therapy has been noted to promote cell proliferation and growth in many different cell types shown both in vitro and in vivo. Currently, treatment regimens are used in the clinic for a variety of ailments, including wound healing. However, most protocols treat an anatomical site without consid...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600025
更新日期:2016-12-01 00:00:00
abstract::The above article from the Journal of Biophotonics, published online on 9 January 2018 in Wiley Online Library as Early View (https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201700354), and in Volume 11, e201700354, has been retracted by agreement between the authors (Hong Zhong, Lin Lu, Yuanhao Wang, Hongxing Ya...
journal_title:Journal of biophotonics
pub_type: 杂志文章,撤回出版物
doi:10.1002/jbio.201890133
更新日期:2020-04-01 00:00:00
abstract::Photodynamic therapy (PDT) uses photosensitizers and visible light in combination with molecular oxygen to produce reactive oxygen species (ROS) that kill malignant cells by apoptosis and/or necrosis, shut down the tumor microvasculature and stimulate the host immune system. The excited singlet state of oxygen (1 O2 )...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.201600055
更新日期:2016-12-01 00:00:00
abstract::We investigate the optical wavelength dependence in quantitative photoacoustic (QPA) assessment of red blood cell (RBC) aggregation and oxygen saturation (sO2 ) during pulsatile blood flow. Experimentally, the pulsatile flow was imaged with a 700 to 900 nm laser using the VevoLAZR. Theoretically, the photoacoustic (PA...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700300
更新日期:2018-08-01 00:00:00
abstract::Localization microscopy methods like Stochastic Optical Reconstruction Microscopy (STORM) are very well suited for exploring clustering of proteins, as the data inherently provide a list of molecular coordinates. Here we use state-of-art cluster analysis algorithms (DBSCAN) to explore the clustering behaviour of diffe...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800080
更新日期:2019-03-01 00:00:00
abstract::The fluorescence lifetime of nicotinamide adenine dinucleotide (NADH) is commonly used in conjunction with the phasor approach as a molecular biomarker to provide information on cellular metabolism of autofluorescence imaging of cells and tissue. However, in the phasor approach, the bound and free lifetime defining th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900156
更新日期:2019-11-01 00:00:00
abstract::Molecular bioswitches offer an invaluable asset in the shift from systemic to targeted treatments. Within the growing arsenal of switches are imaging probes that functionalize only in given locations or situations. Acetate esters are a common fluorescent example, known to activate upon interaction with esterases. Fluo...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700084
更新日期:2018-02-01 00:00:00
abstract::We introduce a unique method for laser-propelling individual cells over distances of 10s of cm through stationary liquid in a microfluidic channel. This is achieved by using liquid-filled hollow-core photonic crystal fiber (HC-PCF). HC-PCF provides low-loss light guidance in a well-defined single mode, resulting in hi...
journal_title:Journal of biophotonics
pub_type: 社论
doi:10.1002/jbio.201200180
更新日期:2013-09-01 00:00:00
abstract::Hyperspectral imaging is a promising technique for resection margin assessment during cancer surgery. Thereby, only a specific amount of the tissue below the resection surface, the clinically defined margin width, should be assessed. Since the imaging depth of hyperspectral imaging varies with wavelength and tissue co...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900086
更新日期:2019-11-01 00:00:00
abstract::Highly emissive europium complexes with specific endoplasmic reticulum localization potential includes several advantages such as fast uptake, long resident lifetime, low dosage requirement, low cytotoxicity and highly emissive two-photon induced f-f emission imaging. ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.200910052
更新日期:2009-12-01 00:00:00
abstract::Cardiomyocytes derived from human pluripotent stem cells are a promising tool for disease modeling, drug compound testing, and cardiac toxicity screening. Bio-image segmentation is a prerequisite step in cardiomyocyte image analysis by digital holography (DH) in microscopic configuration and has provided satisfactory ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800116
更新日期:2018-12-01 00:00:00
abstract::We developed an optical imaging method based on a feedback principle in which the specific scan pattern is adapted according to the shape of the sample. The feedback approach produces nanometer-resolved 3D images of very small and moving features in live cells in seconds. We show images of microvilli in live cultured ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201100002
更新日期:2011-06-01 00:00:00
abstract::The aim of this work was to assess the antimicrobial efficacy on Pseudomonas aeruginosa of nanomicelles loaded with curcumin (CUR) alone and activated by blue laser light in an antimicrobial photodynamic therapy (APDT) approach. First, free CUR in liquid suspension and loaded in three amphiphilic nanomicelles (CUR-DAP...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000350
更新日期:2020-11-05 00:00:00
abstract::We suggest to use a combination of optical tweezers and single-image quantitative differential interference contrast (DIC) emulated by a spatial light modulator (SLM) to study physiological shape changes in thrombocytes after activation and demonstrate the effectiveness of this system for the given task. A specially d...
journal_title:Journal of biophotonics
pub_type: 社论
doi:10.1002/jbio.201100010
更新日期:2011-09-01 00:00:00
abstract::As the field of tissue engineering evolves, there will be an increasingly important need to visualize and track the complex dynamic changes that occur within three-dimensional constructs. Optical coherence tomography (OCT), as an emerging imaging technology applied to biological materials, offers a number of significa...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.200910048
更新日期:2009-11-01 00:00:00
abstract::We present a novel silicon photonic biosensor using phase-shifted Bragg gratings in a slot waveguide. The optical field is concentrated inside the slot region, leading to efficient light-matter interaction. The Bragg gratings are formed with sidewall corrugations on the outside of the waveguide, and a phase shift is i...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300012
更新日期:2013-10-01 00:00:00
abstract::The effect of cetyl-trimethylammonium bromide (CTAB) on enhancing the fluorescence resonance energy transfer (FRET) between two dye-conjugated DNA strands was studied using fluorescence emission spectroscopy and dynamic light scattering (DLS). For hybridized DNA where one strand is conjugated with a TAMRA donor and th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500221
更新日期:2016-01-01 00:00:00
abstract::We have developed a reflection-mode switchable subwavelength Bessel-beam (BB) and Gaussian-beam (GB) photoacoustic microscopy (PAM) system. To achieve both reflection-mode and high resolution, we tightly attached a very small ultrasound transducer to an optical objective lens with numerical aperture of 1.0 and working...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800215
更新日期:2019-02-01 00:00:00
abstract::A novel hybrid method which combines sub-wavelength-scale phase measurements and pixel-scale displacement tracking for robust strain mapping in compressional optical coherence elastography is proposed. Unlike majority of OCE methods it does not rely on initial reconstruction of displacements and does not suffer from t...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500203
更新日期:2016-05-01 00:00:00
abstract::Laser treatment on a large size of prostate gland often encounters significant bleeding that can prolong the entire procedure and cause urinary complications. The current study investigates the feasibility of dual-wavelength (532 and 980 nm) application to achieve rapid hemostasis for 532-nm laser prostatectomy. Porci...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700192
更新日期:2018-04-01 00:00:00
abstract::The photodetachment of Br(-), I(-) and OH(-) in aqueous solution is studied by 2- and 3-pulse femtosecond spectroscopy. The UV excitation leads to fast electron separation followed by formation of a donor-electron pairs. An additional repump pulse is used for secondary excitation of the intermediates. The 3-pulse tech...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000099
更新日期:2011-03-01 00:00:00
abstract::Human immunodeficiency virus (HIV-1) infection remains a major health problem despite the use of highly active antiretroviral therapy (HAART), which has greatly reduced mortality rates. Due to the unavailability of an effective vaccine and treatment that would completely eradicate the virus in infected individuals, th...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600217
更新日期:2017-10-01 00:00:00
abstract::Conventional histopathology is rapidly shifting towards digital integration. Will microscopes (and pathologists) soon be obsolete? Or are we dealing with just another image modality that leaves the core of tissue diagnosis intact? This article provides an overview of current digital pathology applications and research...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.201100103
更新日期:2012-04-01 00:00:00
abstract::In photoacoustic tomography (PAT), a tunable laser typically illuminates the tissue at multiple wavelengths, and the received photoacoustic waves are used to form functional images of relative total haemoglobin (rHbT) and blood oxygenation saturation (%sO2 ). Due to measurement errors, the estimation of these paramete...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000368
更新日期:2020-12-30 00:00:00
abstract::Previous studies for melanin visualization in the retinal pigment epithelium (RPE) have exploited either its absorption properties (using photoacoustic tomography or photothermal optical coherence tomography [OCT]) or its depolarization properties (using polarization sensitive OCT). However, these methods are only sui...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201900153
更新日期:2019-12-01 00:00:00
abstract::Endoscopy has been widely used in biomedical imaging and integrated with various optical and acoustic imaging modalities. Photoacoustic imaging (PAI), one of the fastest growing biomedical imaging modalities, is a noninvasive and nonionizing method that owns rich optical contrast, deep acoustic penetration depth, mult...
journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.202000217
更新日期:2020-12-01 00:00:00