Abstract：
:We have reported previously that the human promyelocytic leukemia cell line HL-60, in its undifferentiated state, is devoid of cytoplasmic intermediate filament proteins and nuclear lamins A and C, but does express lamin B. Using immunofluorescence and immunoblotting techniques, we have further investigated the expression of vimentin and lamins A and C during differentiation of these tumor cells along the macrophage or granulocytic pathway in response to the inducing effects of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) or dimethyl sulfoxide. Our results show that, while the expression of lamin B remains largely unchanged, the synthesis of vimentin and lamins A and C is dramatically enhanced during the maturation of HL-60 cells along both hemopoietic pathways. Northern blot analysis of cellular RNAs isolated from untreated and TPA-treated HL-60 cell populations as well as from control HeLa cells was performed using two oligonucleotides, one complementary to the 5' region common to human lamin A/C mRNAs and the other to the 5' region of hamster vimentin mRNA. Very low but still detectable amounts of vimentin and lamin A/C mRNAs were found in untreated HL-60 cell population, in accordance with the detection of small quantities of vimentin and lamins A and C in these populations. This is probably due to the presence of a small number of spontaneously differentiating cells. On the other hand, strong signals comparable to those obtained with RNA from control HeLa cells were detected for the three mRNA species from TPA-treated cells.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Paulin-Levasseur M,Giese G,Scherbarth A,Traub Psubject
Has Abstractpub_date
1989-12-01 00:00:00pages
453-61issue
2eissn
0171-9335issn
1618-1298journal_volume
50pub_type
杂志文章abstract：:Three-dimensional collagen lattice cultures of fibroblasts mimic the in vivo situation better than monolayer cultures. Here, skin fibroblasts from scleroderma patients and healthy controls were cultivated in collagen lattices, and the effects of recombinant human gamma-interferon (IFN-gamma) on these cultures investig...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 ha...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.08.003
更新日期：2010-12-01 00:00:00
abstract：:In the literature the question of whether a system structurally and functionally related to the barrier function of the tight junctions (TJs) of polarized epithelia exists in the epidermis has been and still is controversially discussed. We have systematically addressed this question in a study of the granular layer o...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00434
更新日期：2004-12-01 00:00:00
abstract：:Seminal roots are initiated at the scutellar node during maize (Zea mays L.) embryo development. The maize mutant rtcs (rootless concerning crown and seminal roots) does not initiate seminal roots while its wild-type siblings form on average 2.9 seminal roots per seedling. In this study, proteome profiles of 25-day-ol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2009.11.014
更新日期：2010-02-01 00:00:00
abstract：:In Drosophila embryos, the dorsal gene, a member of the rel family of transcription factors, is a maternal gene required for dorsal/ventral axis specification and mesoderm and neural migration and differentiation. In this report, the presence and distribution of Xrel-1 protein, a Xenopus homologue of the rel family of...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Various drugs added to the culture medium of rabbit mammary gland were assayed for their capacity to affect the lactogenic and the mammogenic activities of prolactin. Three lysosomotropic agents NH4Cl, chloroquine and methylamine which were previously demonstrated to inhibit the degradation of the hormone-receptor com...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:Alternative splicing in the extracellular domain is a characteristic feature of members of the fibroblast growth factor receptor (FGFR) family. This splicing event generates receptor variants, which differ in their ligand binding specificities. A poorly characterized splice variant is FGFR1-IIIb, recently found to be ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00355
更新日期：2004-02-01 00:00:00
abstract：:Plastids, an essential group of plant cellular organelles, proliferate by division to maintain continuity through cell lineages in plants. In recent years, it was revealed that the bacterial cell division protein FtsZ is encoded in the nuclear genome of plant cells, and plays a major role in the plastid division proce...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00318
更新日期：2003-06-01 00:00:00
abstract：:Cytolocalization of stomatin, an integral membrane protein also called erythrocyte band 7.2b, was investigated in a human epithelial cell line in which the expression of this protein is up-regulated after treatment with interleukin-6 and dexamethasone. A monoclonal antibody against stomatin was used to perform immunof...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Interleukin-1 beta (Il-1 beta) and interleukin-1 alpha (Il-1 alpha) were shown to act as motility factors for the human breast carcinoma cell lines SK-BR-3 and ZR-75-1 in vitro. Both cytokines induced transition from the stationary to the motile phenotype (spreading). Il-1 beta stimulated translocation, shape change a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:A new, two step method is described for the isolation of megakaryocytes from bone marrow. By means of centrifugation in a PercollR density gradient megakaryocytes can be enriched by factor 68 or 24 starting from rat or guinea pig bone marrow, respectively. The purity of the megakaryocytes can be improved ca. 4 fold if...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-06-01 00:00:00
abstract：:The exhaustion of metabolically poisoned muscle fibres is accompanied by a one hundred-fold increase in potassium conductance. Under these conditions a normal membrane capacity was measured. Extracellular markers (lanthanum, ferritin) were traced in the transverse tubular system but not in the myoplasm or the sarcopla...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:The fine structure of a joint receptor (R10) in a spider leg (Zygiella x-notata) was examined with light and electron microscopy. The R10 receptor consists of a compact ganglion which is situated near the dorsal joint membrane of the femur/patella joint. Each of the ten sensory cells comprising the ganglion sends one ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:Pinocytosis was studied in cultured human skin fibroblasts in order to obtain more information about uptake in these cells of non-specific ligands with different net surface charge. The fibroblasts were obtained from a normal person and a patient homozygous for familial hypercholesterolemia. No ultrastructural differe...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-06-01 00:00:00
abstract：:mRNA translation is regulated by diverse mechanisms that converge at the initiation and elongation steps to determine the rate, profile, and localization of proteins synthesized. A consistently relevant feature of these mechanisms is the spatial re-distribution of translation machinery, a process of particular importa...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2018.02.002
更新日期：2018-04-01 00:00:00
abstract：:It was previously shown that avidin, a glycoprotein secreted in vivo by chicken oviduct, is produced by cultured transformed or damaged chicken embryo fibroblasts [27]. This report demonstrates synthesis and secretion of large amounts of avidin by macrophages isolated from chicken yolk sac. Avidin was secreted to the ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Sertoli cells in culture synthesize two membrane-associated proteoglycans (PGs) containing as glycosaminoglycan (GAG) moieties either chondroitin sulfate (CS) or CS and heparan sulfate (HS); the latter PG is, therefore, referred to as the mixed PG. To determine if these PGs are randomly distributed on the cell surface...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:The differences in the content of trace elements (Fe, Cu, Zn, Br, Rb and Sr) in phasic and tonic scallop adductor muscles have been revealed by using X-ray fluorescence analysis. The phasic (yellow) muscles have been found to be richer in Fe and Cu while more deficient in Zn and Br as compared to the tonic (white) mus...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:The amoeba Dictyostelium discoideum produces and secretes multilamellar bodies (MLBs) mainly composed of amoebal membranes upon digestion of bacteria. After their secretion, the fate of these MLBs remains unknown. The aim of this study was to determine if protozoa can internalize and digest secreted D. discoideum MLBs...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2017.10.002
更新日期：2017-12-01 00:00:00
abstract：:We have used plant root tips frozen under high pressure in conjunction with freeze-fracture electron microscopy a) to evaluate the quality of freezing of unfixed, non-cryoprotected tissues obtainable with this method, b) to examine the structure of cells frozen under high pressure, c) to evaluate the usefulness of hig...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:The evolutionary conserved transmembrane protein Crumbs (Crb) regulates morphogenesis of photoreceptor cells in the compound eye of Drosophila and prevents light-dependent retinal degeneration. Here we examine the role of Crb in the ocelli, the simple eyes of Drosophila. We show that Crb is expressed in ocellar photor...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2012.03.006
更新日期：2012-09-01 00:00:00
abstract：:Podosomes and the immune synapse are integrin-mediated adhesive structures that share a common ring-like morphology. Both podosomes and immune synapses have a central core surrounded by a peripheral ring containing talin, vinculin and paxillin. Recent progress suggests significant parallels between the regulatory mech...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2008.01.011
更新日期：2008-09-01 00:00:00
abstract：:Exocytosis of synaptic vesicles (SV) results in the surface exposure of lumenal epitopes of SV proteins. We have recently described the use of antibodies directed against the lumenal N-terminus of synaptotagmin I (Sytlum-Abs) to morphologically monitor exo-endocytic recycling of SVs. We report here that a radioimmunoa...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:The present study was undertaken because of the paucity of information on peroxisomes in molluscs and the increasing importance of these organisms as sensitive indicators of environmental pollution. Peroxisomes were identified by electron microscopy in all three main cell types of the digestive gland of the bivalve mo...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:The importance of intact microtubules in the processing, storage and regulated secretion of von Willebrand factor (vWf) from Weibel-Palade bodies in endothelial cells was investigated. Human umbilical vein endothelial cells treated for 1 h with colchicine (10(-6) M) or nocodozole (10(-6) M) lost their organized microt...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:The uptake mechanism of homologous IgG and immune complex, and the participation of coated vesicles in this process were studied in rat peritoneal macrophages. Peroxidase-antiperoxidase (PAP) immune complex produced in rat, and purified rat IgG adsorbed to gold particles (IgG-Au) were used as ligands. Freshly collecte...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:Herein we report that Gts1p fused with green-fluorescent protein (GFP) is localized in the cortical actin patch besides nuclei in yeast and the cortical Gts1p changed its position together with the patch depending on the cell-cycle phase, while nuclear Gts1p accumulated predominantly in the budding phase. Whereas Gts1...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2007.02.005
更新日期：2007-05-01 00:00:00
abstract：:The fetoacinar pancreatic (FAP) protein is a specific component of the human exocrine pancreas associated with the differentiation and proliferation of acinar cells. FAP expression is enhanced in cases of pancreatic exocrine cancer and it is found in relatively high concentrations in pathological pancreatic juices. Ho...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:This report describes the characterization of the nuclear lamin CeLam-1 of the nematode Caenorhabditis elegans by molecular analysis of the corresponding complete cDNA and gene sequences. The primary structure of CeLam-1, representing only the third non-vertebrate lamin sequence currently known, follows essentially th...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
