Abstract:
:Sertoli cells in culture synthesize two membrane-associated proteoglycans (PGs) containing as glycosaminoglycan (GAG) moieties either chondroitin sulfate (CS) or CS and heparan sulfate (HS); the latter PG is, therefore, referred to as the mixed PG. To determine if these PGs are randomly distributed on the cell surface, Sertoli cell monolayers were treated with chondroitinase ABC, and then the remaining PGs were analyzed by DEAE-Sephacel chromatography. The results obtained with Sertoli cell monolayers show that the CS of the mixed PG is degraded by chondroitinase, while the CS-PG is not degraded. In contrast, chondroitinase treatment of Sertoli cells in suspension shows that both the mixed PG and the CS-PG are degraded. From this, it is inferred that the mixed PG is apically oriented and the CS-PG is basolaterally oriented. Studies of the adhesion of germ cells to Sertoli cell monolayers give further support to the apical location of the mixed PG and suggest that its HS moiety is involved in the attachment of germ cells to Sertoli cells.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Rodriguez JP,Minguell JJsubject
Has Abstractpub_date
1992-12-01 00:00:00pages
348-51issue
2eissn
0171-9335issn
1618-1298journal_volume
59pub_type
杂志文章abstract::Various drugs added to the culture medium of rabbit mammary gland were assayed for their capacity to affect the lactogenic and the mammogenic activities of prolactin. Three lysosomotropic agents NH4Cl, chloroquine and methylamine which were previously demonstrated to inhibit the degradation of the hormone-receptor com...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract::Centaurin alpha-1 is a high-affinity PtdIns(3,4,5)P3-binding protein enriched in brain. Sequence analysis indicates centaurin alpha-1 contains two pleckstrin homology domains, ankyrin repeats and an Arf GAP homology domain, placing it in the AZAP family of phosphoinositide-regulated Arf GAPs. Other members of this fam...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00416
更新日期:2004-10-01 00:00:00
abstract::RNA interference (RNAi) is a powerful tool for functional genetic studies in model organisms and mammalian cells. To facilitate rapid construction of gene knockdown constructs and RNAi libraries for known genes of mammalian cells, a new and simple strategy to produce small interfering RNA (siRNA) expression vectors wi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.10.009
更新日期:2006-05-01 00:00:00
abstract::PinX1 was originally identified as a Pin2/TRF1-interacting protein that suppresses telomerase activity via its telomerase inhibitor domain (TID) and regulates the nucleolar localization of TRF1 in telomerase-positive cells. In addition to its telomeric localization, PinX1 can be found in the nucleoli of human cells. O...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2010.05.005
更新日期:2010-10-01 00:00:00
abstract::Proteins that are concentrated in specific compartments of the endomembrane system in order to exert their organelle-specific function must possess specific localization signals that prevent their transport to distal regions of the exocytic pathway. Some resident proteins of the endoplasmic reticulum (ER) that are kno...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70025-4
更新日期:2000-04-01 00:00:00
abstract::Embryonic stem (ES) cells are omnipotent; they can differentiate into every cell type of the body. The development of culture conditions that allow their differentiation has made it conceivable to produce large numbers of cells with lineage-specific characteristics in vitro. Here, we describe a method by which murine ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2007.07.001
更新日期:2007-12-01 00:00:00
abstract::In Drosophila embryos, the dorsal gene, a member of the rel family of transcription factors, is a maternal gene required for dorsal/ventral axis specification and mesoderm and neural migration and differentiation. In this report, the presence and distribution of Xrel-1 protein, a Xenopus homologue of the rel family of...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::mRNA translation is regulated by diverse mechanisms that converge at the initiation and elongation steps to determine the rate, profile, and localization of proteins synthesized. A consistently relevant feature of these mechanisms is the spatial re-distribution of translation machinery, a process of particular importa...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2018.02.002
更新日期:2018-04-01 00:00:00
abstract::Pinocytosis was studied in cultured human skin fibroblasts in order to obtain more information about uptake in these cells of non-specific ligands with different net surface charge. The fibroblasts were obtained from a normal person and a patient homozygous for familial hypercholesterolemia. No ultrastructural differe...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::Earlier studies have indicated that Jun/AP-1 activity is associated with, and probably required for apoptosis induction by DNA-damaging and stress-inducing agents in human myeloid cells. To investigate this possibility, we examined the capacity of continuous treatments with etoposide (10 microM) and camptothecin (0.4 ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70001-1
更新日期:2000-01-01 00:00:00
abstract::The intracellular storage of zinc in Malpighian tubules of Drosophila hydei was studied by X-ray microanalysis of freeze-dried cryosections. Mass dense vacuoles in the proximal region of the anterior larval Malpighian tubule cells were found to accumulate zinc, not sodium. The zinc content was enhanced considerably af...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
abstract::An increase in light intensity induces a depolarization in retinal ON-bipolar cells via a reduced glutamate release from presynaptic photoreceptor cells. The underlying transduction cascade in the dendritic tips of ON-bipolar cells involves mGluR6 glutamate receptors signaling to TRPM1 proteins that are an indispensab...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2015.06.005
更新日期:2015-07-01 00:00:00
abstract::Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::Cell cycle kinetics and energy metabolism of Ehrlich ascites tumor cells grown in glucose-deprived medium supplemented with uridine, were investigated in order to extend our knowledge about the significance of the metabolic conversion of glucose for cell cycle progression of these cells. Viability (dye exclusion test)...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::Malignant melanoma is an aggressive form of skin cancer with an increasing incidence worldwide. One way to address the pathology of the disease is through molecular research. In addition to the analysis of melanoma-relevant signaling pathways, the investigation of important transcription factors is a fundamental objec...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2013.10.003
更新日期:2014-01-01 00:00:00
abstract::Centromeres support the assembly of the kinetochore on every chromosome and are therefore essential for the proper segregation of sister chromatids during cell division. Centromere identity is regulated epigenetically through the presence of the histone H3 variant CENP-A. CENP-A regulation and incorporation specifical...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2011.04.016
更新日期:2011-10-01 00:00:00
abstract::This study reports the ultrastructural detection of fucosyl residues at the surface of axenically grown roots of Zea mays. The method used involved sequential binding of UeA lectin to the root and coupling of the bound UeA with fucosyl ferritin. Superficial dense ferritin labelling was found in the slime droplet enshe...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::The major families of proteoglycans in human arterial tissue have been localized and characterized by electron microscopy. After staining with the polycationic dye cuprolinic blue in the presence of a critical electrolyte concentration, three differently sized populations of proteoglycan-cuprolinic blue precipitates a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::The uptake mechanism of homologous IgG and immune complex, and the participation of coated vesicles in this process were studied in rat peritoneal macrophages. Peroxidase-antiperoxidase (PAP) immune complex produced in rat, and purified rat IgG adsorbed to gold particles (IgG-Au) were used as ligands. Freshly collecte...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::The murine recessive yellow (Mc1r(e)) is a loss-of-function mutation in the receptor for alpha-melanocyte-stimulating hormone, melanocortin receptor 1 (Mc1r) and produces yellow coats by inducing pheomelanin synthesis in hair follicular melanocytes. However, it is not known whether the Mc1r(e) mutation affects the pro...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2007.03.007
更新日期:2007-06-01 00:00:00
abstract::Initiation of eukaryotic DNA replication is achieved by the sequential binding of different proteins to origins of DNA replication. Using EGFP-tagged initiator proteins and immunofluorescence techniques we found that most of the ORC and the MCM subunits are localised at centrosomes and are colocalised with the polo-li...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2006.09.002
更新日期:2007-01-01 00:00:00
abstract::We have examined the importance of cell surface heparan sulfate proteoglycans (HSPG) in fibroblast growth factor (FGF) signaling. 3T3 cells grown under conventional conditions were much more sensitive to FGF-2 compared to FGF-1. However, cells were equally sensitive to FGF-1 and FGF-2 using conditions which reduced th...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::A method using proteins covalently coupled to glass surfaces has been applied to studies on the differentiation of neuroblasts. Neuroblastoma cells from clones N 18 and NIE 115 adhere to surfaces coated with fibronectin or laminin and extend rapidly growth cone-containing neurites. Some lectin-coated surfaces are also...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::The central feature of fluid and electrolyte secretion by salivary acinar cells is transepithelial Cl- movement as a driving force for the secretion. However, little is known about the membrane localization and regulation by agonists of various anion channels. To characterize the anion transport and fluid secretion, w...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70021-7
更新日期:2000-03-01 00:00:00
abstract::Caldesmon and calponin are two proteins that are characteristic of vertebrate smooth muscle. In invertebrates, caldesmon has only been studied in some molluscan muscles, and no previous references to calponin have been found. The aim of this paper was to investigate the presence and distribution of caldesmon and calpo...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Rho GTPases are ubiquitously expressed across the eukaryotes where they act as molecular switches participating in the regulation of many cellular processes. We present an inventory of proteins involved in Rho-regulated signaling pathways in Dictyostelium discoideum that have been identified in the completed genome se...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2006.04.011
更新日期:2006-09-01 00:00:00
abstract::Hyaluronan is a straight chain, glycosaminoglycan polymer of the extracellular matrix composed of repeating units of the disaccharide [-D-glucuronic acid-beta1,3-N-acetyl-D-glucosamine-beta1,4-]n. Hyaluronan is synthesized in mammals by at least three synthases with products of varying chain lengths. It has an extraor...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2006.05.009
更新日期:2006-08-01 00:00:00
abstract::In the cell cycle the transition from G2 phase to cell division (M) is strictly controlled by protein phosphorylation-dephosphorylation reactions effected by several protein kinases and phosphatases. Although much indirect and direct evidence point to a key role of protein phosphatase 2A (PP2A) at the G2/M transition,...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.04.002
更新日期:2005-08-01 00:00:00
abstract::The inner core of lipopolysaccharide (LPS) structures in Gram-negative bacteria is considered a highly conserved region. The sugar connecting the membrane-associated lipid A moiety with the hydrophilic saccharide moiety, 3-deoxy-alpha-d-manno-oct-2-ulosonic acid (Kdo) is present in every LPS molecule investigated but ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2009.10.002
更新日期:2010-01-01 00:00:00
abstract::The ESCRT pathway functions at different subcellular membranes to induce their negative curvature, and it has been largely characterized in model eukaryotes belonging to Opisthokonta. But searches of the genomes of many nonopisthokonts belonging to various supergroups indicate that some of them may harbour fewer ESCRT...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.11.004
更新日期:2018-01-01 00:00:00