Abstract:
:We have examined the importance of cell surface heparan sulfate proteoglycans (HSPG) in fibroblast growth factor (FGF) signaling. 3T3 cells grown under conventional conditions were much more sensitive to FGF-2 compared to FGF-1. However, cells were equally sensitive to FGF-1 and FGF-2 using conditions which reduced the effect of endogenous HSPG. Addition of heparin, or treatment with chlorate, an inhibitor of proteoglycan sulfation, resulted in enhanced or reduced growth factor response, respectively, and eliminated the differences between FGF-1 and FGF-2. HSPGs isolated from trypsin digests of 3T3 cells had a much higher affinity for FGF-2 compared to that for FGF-1 when analyzed by affinity chromatography. Glycosaminoglycan chains or core protein fragments derived from the HSPG failed to show the same high apparent affinity for FGF-2, suggesting that an intact proteoglycan structure was important for the high FGF-2 affinity. Addition of HSPG ectodomains, isolated from cultured 3T3 cells or produced as recombinant molecules, to chlorate-treated cultures of 3T3 cells inhibited the mitogenic activity of FGF-2 and eliminated the effect of heparin as a potentiator of either growth factor. These results support the idea that the cell-associated HSPG is an integral component of the FGF signaling system and in 3T3 cells contributes to the increased sensitivity of these cells to FGF-2 compared to FGF-1. Since isolated ectodomains of HSPG inhibited rather than stimulated the mitogenic response of the FGFs, the proper anchoring of the HSPG in the cell membrane appears to be important for a stimulatory effect.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Zhou FY,Owens RT,Hermonen J,Jalkanen M,Höök Msubject
Has Abstractpub_date
1997-06-01 00:00:00pages
166-74issue
2eissn
0171-9335issn
1618-1298journal_volume
73pub_type
杂志文章abstract::In response to hypotonic stress, cells of the inner medullary collecting duct (IMCD) undergo swelling followed by a regulatory volume decrease (RVD) and a transient release of organic osmolytes such as sorbitol. In this study, we tested the hypothesis whether membrane recycling is involved in the latter process. There...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::The 90-kDa heat-shock protein, hsp90, is an abundant and conserved protein located predominantly in the cytoplasm. Previous reports have localized a portion of the hsp90 to microtubules and to microfilaments. Here, we show that colocalization of hsp90 with microtubules is seen after long-term but not short-term fixati...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::While many studies of erythropoiesis have focused on interactions between developing cells and the extracellular matrix, scant attention has been paid to the role of erythroid-macrophage interactions in this process. Murine erythroleukemia (MEL) cell lines have been used extensively in these studies to define erythroi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::Monoclonal antibodies were used to define cell surface antigens which are present on rat hepatocytes but are absent from hepatoma cells. One monoclonal antibody, referred to as Be 9.2, recognizes a major component of purified rat liver plasma membranes with a Mr of 110 000. This antigen (gp110) was not found in the tr...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Filamentous networks were isolated from rat liver and studied by electron microscopy after negative staining with uranyl acetate. Highly purified preparations of networks were used to obtain material for immunological studies, but morphological studies were made more satisfactorily on preparations purified only by suc...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::The mutation raised (rsd, 3-95.4) of Drosophila melanogaster causes flightlessness as a consequence of abnormalities in the fibrillar flight muscles (FFMs). In this muscle type actin III is neither synthesized nor accumulated while adult tubular muscles of rsd flies are indistinguishable from wildtype. This paper demo...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Initial integrin-mediated cell-matrix adhesions (focal complexes) appear underneath the lamellipodia, in the regions of the "fast" centripetal flow driven by actin polymerization. Once formed, these adhesions convert the flow behind them into a "slow", myosin II-driven mode. Some focal complexes then turn into elongat...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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abstract::Mitotic PtK1 spindles were UV irradiated (285 nm) during metaphase and anaphase between the chromosomes and the pole. The irradiation, a rectangle measuring 1.4 x 5 microns parallel to the metaphase plate, severed between 90 and 100% of spindle microtubules (MTs) in the irradiated region. Changes in organization of MT...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) exhibit the potential to repair a wide variety of injured adult tissues. The migration capability of MSCs is an important determinant of the efficiency of MSC transplant therapy. MicroRNAs (miRNAs) are increasingly implicated in regulating the migration of MSCs. Herein, we show that the e...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2016.06.005
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Zymogen granules (ZG) of rat pancreas have been isolated by the procedure of Pâquet et al. The granules lysed when exposed to alkaline pH (pH 8.2), and their membranes could be subfractionated by centrifugation on a sucrose gradient. Four discrete types of membranes corresponding to densities of 1.105, 1.085, 1.075, a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::The presence of endo- and exoproteolytic activity in peroxisomes was detected in cell organelles purified from pea leaves. By PAGE using different exopeptidase substrates (L-aa-beta NA), one leucine aminopeptidase (AP) was found in peroxisomes. The peroxisomal AP was characterized as a serine protease and had a maxima...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
abstract::The plasma membrane of nucleated erythrocytes contains a microtubular marginal band which appears to be associated with the plasma membrane skeleton. In this report, we identify two families of cytoskeletal proteins which may be involved in such an association. These proteins, of molecular mass 78 kDa and 48 kDa on SD...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::Podosomes and the immune synapse are integrin-mediated adhesive structures that share a common ring-like morphology. Both podosomes and immune synapses have a central core surrounded by a peripheral ring containing talin, vinculin and paxillin. Recent progress suggests significant parallels between the regulatory mech...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2008.01.011
更新日期:2008-09-01 00:00:00
abstract::Procaine-treated eggs can be penetrated by more than one spermatozoon. Supernumerary male pronuclei can fuse with the female one giving raise to multipolar spindles or remain isolated within the egg's cytoplasm forming their own spindle. In all types of multiple mitotic figures (asters and spindles) the distribution o...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::When epithelial cells reach confluency in vitro, a number of energy-requiring activities such as growth and motility are contact-inhibited. We investigated the possible role of the E-cadherin/catenin complex, which acts as an invasion suppressor, in contact inhibition. Three strategies for modulation of the complex we...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::Human HeLa or SkHep1 cells, defective in intercellular communication through gap junctions, were transfected with coding sequences of murine connexin40 (Cx40) and -43. The transfected cells were restored in gap junctional coupling as shown by 100-fold increased electrical conductance. When studied by the double whole-...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::The induction of pollen embryogenesis in Capsicum annuum L. has been studied at the cellular level using various in situ approaches with several molecular probes for DNA, RNA and proteins. The late vacuolated microspore and the young bicellular pollen grain are stages of gametophytic development in which embryogenesis...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
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journal_title:European journal of cell biology
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doi:10.1016/j.ejcb.2007.03.007
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2010.08.003
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70021-7
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00128
更新日期:2000-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
abstract::Specific antibodies to a protein designated P1 (Mr approximately equal to 63,000), which is specifically altered in mutants resistant to the microtubule inhibitor podophyllotoxin, bind to mitochondria in cells of various vertebrate and invertebrate species (Eur. J. Cell Biol. 44, 278-285 (1987); Can. J. Biochem. Cell ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00