Abstract:
:The mutation raised (rsd, 3-95.4) of Drosophila melanogaster causes flightlessness as a consequence of abnormalities in the fibrillar flight muscles (FFMs). In this muscle type actin III is neither synthesized nor accumulated while adult tubular muscles of rsd flies are indistinguishable from wildtype. This paper demonstrates ultrastructural defects in rds FFMs and extends the biochemical comparison of adult wildtype and rsd muscles to larval muscles and to embryo cells differentiating in culture. The FFMs of mature rsd flies contain thick filaments in irregular bundles, but no thin filaments. Normal Z-discs are virtually absent. Instead, a large number of Z-disc residues are present in stacks attached to short filaments on either side. In newly emerged rsd flies the disorganization is less pronounced. The adult tubular muscles and the supercontracting muscles of third-instar larvae of rsd can ultrastructurally not be distinguished from wildtype. The present biochemical results indicate that not only FFMs of mature and newly emerged adults are affected by the rsd genotype. Synthesis of actin III is not detectable in rsd FFMs which corresponds to the heavy structural defects. In addition to the lack of actin III synthesis in rsd FFMs, three unidentified proteins (52 kDa, 80 kDa, 90 kDa) which are specific for wildtype FFMs are also not synthesized in rsd flies. Among all other muscle types studied, all of which are morphologically unaffected, only adult tubular muscle of rsd genotype showed no biochemical effect. Larval supercontracting muscle as well as embryo cells differentiating in culture failed to synthesize actin III in the case of rsd cells.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Lang AB,Reinhardt CA,Eppenberger HM,Wyss Csubject
Has Abstractpub_date
1985-01-01 00:00:00pages
8-16issue
1eissn
0171-9335issn
1618-1298journal_volume
36pub_type
杂志文章abstract::Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::Fas ligand can both be used by the immune system to initiate cell death, and be used by non-lymphoid cells to evade death. Recent work has shown that Fas ligand is differentially sorted in different cell types. Here we present the viewpoint that the differential sorting plays an important part in determining the role ...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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abstract::It has been known for many years that trypanosomatids require exogenous essential growth factors in order to divide. Two surface domains are involved in starting nutrient endocytosis: the flagellar pocket and the cytostome. Although the flagellar pocket plays a fundamental role in the endocytic process occurring in se...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::Recently, we have described the developmental expression of the small heat shock proteins (sHsps) Hsp27/HspB1 and alphaB-crystallin/HspB5 in different tissues of pigs from almost full-term foetuses to three years old adults (P. Tallot, J. F. Grongnet, J. C. David, Biol. Neonate, 83, 281-288, 2003). The data described ...
journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2006.11.005
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::The neural cell adhesion molecule (NCAM) plays a key morphoregulatory role during neural development. Patterns of NCAM expression were investigated in cultured astrocytes exhibiting distinct states of morphological differentiation. In vitro differentiation of confluent primary cultures of astrocytes was enhanced by a ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70021-7
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1078/0171-9335-00346
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::Cytochrome b5 has been visualized by an immunoferritin labeling in a Golgi preparation. When the Golgi apparatus was first disrupted before labeling, 60% of the profiles were clearly labeled with ferritin. This proportion of labeled vesicles was too high to be explained by contamination from other membranes. When the ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
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更新日期:1990-02-01 00:00:00
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