Abstract:
:During maize pollen embryogenesis, a range of multicellular structures are formed. Using different approaches, the "nature" of these structures has been determined in terms of their embryogenic potential. In situ molecular identification techniques for gene transcripts and products, and a novel cell tracking system indicated the presence of embryogenic (embryo-like structures, ELS) and non-embryogenic (callus-like structures, CLS) structures that occurred for short periods within the cultures. Some multicellular structures with a compact appearance generated embryos. RT-PCR and fluorescence in situ hybridization (FISH) with confocal microscopy techniques using specific gene markers of the endosperm (ZmESR2, ZmAE3) and embryo (LTP2 and ZmOCL1, ZmOCL3) revealed "embryo" and "endosperm" potentialities in these various multicellular structures present in the cultures. The results presented here showed distinct and specific patterns of gene expression. Altogether, the results demonstrate the presence of different molecules on both embryonic and non-embryonic structures. Their possible roles are discussed in the context of a parallel between embryo/endosperm interactions in planta and embryonic and non-embryonic structure interrelations under in vitro conditions.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Massonneau A,Coronado MJ,Audran A,Bagniewska A,Mòl R,Testillano PS,Goralski G,Dumas C,Risueño MC,Matthys-Rochon Edoi
10.1016/j.ejcb.2005.02.002subject
Has Abstractpub_date
2005-07-01 00:00:00pages
663-75issue
7eissn
0171-9335issn
1618-1298pii
S0171-9335(05)00062-2journal_volume
84pub_type
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journal_title:European journal of cell biology
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abstract::The induction of pollen embryogenesis in Capsicum annuum L. has been studied at the cellular level using various in situ approaches with several molecular probes for DNA, RNA and proteins. The late vacuolated microspore and the young bicellular pollen grain are stages of gametophytic development in which embryogenesis...
journal_title:European journal of cell biology
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更新日期:1996-04-01 00:00:00
abstract::Lysosomal Ca2+ release channel TRPML1 has been suggested to regulate lysosome size by activating calmodulin (CaM). To further understand how TRPML1 and CaM regulate lysosome size, in this study, we report that inhibiting mTORC1 causes enlarged lysosomes, and the recovery of enlarged lysosomes is suppressed by inhibiti...
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journal_title:European journal of cell biology
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abstract::The platinum-carbon replica technique was applied to rat hepatocyte monolayers to visualize the distribution of the receptor responsible for the clearance of asialoglycoproteins. In a first series of experiments, hepatocytes were mildly fixed, either immediately after transfer to 4 degrees C, or after up to 24 h of in...
journal_title:European journal of cell biology
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更新日期:1993-02-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Sertoli cells in culture synthesize two membrane-associated proteoglycans (PGs) containing as glycosaminoglycan (GAG) moieties either chondroitin sulfate (CS) or CS and heparan sulfate (HS); the latter PG is, therefore, referred to as the mixed PG. To determine if these PGs are randomly distributed on the cell surface...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::Rabbit antisera against homogeneous rat liver thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 1.6.4.5) were prepared and used for immunohistochemical analysis in adult rats. Immunoreactive thioredoxin and thioredoxin reductase were widely distributed in tissues and organs, but va...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2010.08.003
更新日期:2010-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2007.07.001
更新日期:2007-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::We have examined the polarity of microtubules in Sertoli cells of the seminiferous epithelium during testicular development to test the hypothesis that microtubules change their polarity during tight junction formation. Microtubules in a number of polarized epithelial cells, including Sertoli cells, are oriented with ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::Hepatocytoma (HPCT) hybrid cells were obtained by fusion of cultured rat hepatocytes with Fao Reuber hepatoma cells H35 by polyethylene glycol treatment. Surviving cells were cloned in HAT (hypoxanthine-aminopterine-thymidine)/ouabain medium and propagated in cell lines over 80 passages. Morphological criteria were ch...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-08-01 00:00:00
abstract::The aim of this study was to evaluate the influence of peroxisome proliferator-activated receptor γ (PPARγ) on self-renewal of mouse embryonic stem cells (mESCs) in the presence and absence of leukemia inhibitory factor (LIF). We demonstrated that in the presence of LIF, the activation of PPARγ by Rosiglitazone led to...
journal_title:European journal of cell biology
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更新日期:2013-04-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
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更新日期:2006-09-01 00:00:00
abstract::Microbodies containing bipyramidal crystalline nucleoid inclusions occur within every cortical cell in roots of Yucca torreyi. Reaction product deposition attributable to catalase, glycolate oxidase, and urate oxidase activities are cytochemically localized to Yucca root microbodies and classifies them as unspecialize...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::D-amino acid oxidase is expressed to a high level in the yeast Rhodotorula gracilis (0.3% of total cell protein) through induction by D-alanine in a defined growth medium. Monospecific polyclonal antibodies against pure enzyme were obtained. Western blot analysis showed that the enzyme is synthesized as the mature pol...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::Malignant melanoma is an aggressive form of skin cancer with an increasing incidence worldwide. One way to address the pathology of the disease is through molecular research. In addition to the analysis of melanoma-relevant signaling pathways, the investigation of important transcription factors is a fundamental objec...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2013.10.003
更新日期:2014-01-01 00:00:00
abstract::The uptake mechanism of pertussis toxin (PT) in CHO and insulin-producing HIT-T15 cells was studied. By electron microscopy after direct labeling of the toxin with gold particles, PT was found to be taken up by receptor-mediated endocytosis. The presence of active pertussis toxin in the Golgi complex was shown by subc...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
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journal_title:European journal of cell biology
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doi:
更新日期:1994-08-01 00:00:00
abstract::The receptor-mediated endocytosis and intracellular processing of transferrin and mannose receptor ligands were investigated in bone marrow-derived macrophages, fibroblasts and reticulocytes. Mannosylated bovine serum albumin (BSA) conjugated to colloidal gold (Au-man-BSA) or colloidal gold-transferrin (AuTf) were use...
journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1985-03-01 00:00:00