Abstract：
:D-amino acid oxidase is expressed to a high level in the yeast Rhodotorula gracilis (0.3% of total cell protein) through induction by D-alanine in a defined growth medium. Monospecific polyclonal antibodies against pure enzyme were obtained. Western blot analysis showed that the enzyme is synthesized as the mature polypeptide. The localization of the enzyme was investigated by immunoelectron microscopy using the postembedding immunogold technique and by submicroscopic enzyme cytochemistry. D-Amino acid oxidase was detected in peroxisomes, and quantitation of immunoelectron microscopic data indicated that the enzyme is exclusively confined to these organelles. Immunoelectron microscopic observations are in complete agreement with biochemical data showing that the enzyme is not expressed in the absence of D-alanine. Morphometric analysis demonstrated that induction of D-amino acid oxidase synthesis is associated with a 241% increase of peroxisome volume density and with a 31% increase of peroxisome size as compared to cells grown on non-inducing medium.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Perotti ME,Pollegioni L,Pilone MSsubject
Has Abstractpub_date
1991-06-01 00:00:00pages
104-13issue
1eissn
0171-9335issn
1618-1298journal_volume
55pub_type
杂志文章abstract：:Actin, myosin, and the actin-associated proteins tropomyosin, alpha-actinin, vinculin, and villin were localized in acinar cells of rat and bovine pancreas, parotid, and prostate glands by means of immunofluorescent staining of both frozen tissue sections and semithin sections of quick-frozen, freeze-dried, and plasti...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Hyaluronan is a straight chain, glycosaminoglycan polymer of the extracellular matrix composed of repeating units of the disaccharide [-D-glucuronic acid-beta1,3-N-acetyl-D-glucosamine-beta1,4-]n. Hyaluronan is synthesized in mammals by at least three synthases with products of varying chain lengths. It has an extraor...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2006.05.009
更新日期：2006-08-01 00:00:00
abstract：:Seminal roots are initiated at the scutellar node during maize (Zea mays L.) embryo development. The maize mutant rtcs (rootless concerning crown and seminal roots) does not initiate seminal roots while its wild-type siblings form on average 2.9 seminal roots per seedling. In this study, proteome profiles of 25-day-ol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2009.11.014
更新日期：2010-02-01 00:00:00
abstract：:Earlier studies have indicated that Jun/AP-1 activity is associated with, and probably required for apoptosis induction by DNA-damaging and stress-inducing agents in human myeloid cells. To investigate this possibility, we examined the capacity of continuous treatments with etoposide (10 microM) and camptothecin (0.4 ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/S0171-9335(04)70001-1
更新日期：2000-01-01 00:00:00
abstract：:Dermatological diseases range from minor cosmetic problems to life-threatening conditions, as seen in some severe disorders of keratinization and cornification. These disorders are commonly due to abnormal epidermal differentiation processes, which result in disturbed barrier function of human skin. Elucidation of the...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1078/0171-9335-00388
更新日期：2004-12-01 00:00:00
abstract：:The distribution of microtubules and centrosomes was studied during sheep fertilization by electron and immunofluorescence microscopy. Tubulin and centrosomal material was identified with monoclonal anti-alpha-tubulin and MPM-2 antibodies, respectively. In ovulated eggs, microtubules were exclusively found in the meio...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:We examined the secretion of three serum proteins, albumin (RSA), alpha 2 mu-globulin (alpha 2 mu G), and transferrin (Trf), in the isolated perfused liver. Within 4 h of perfusion, only 20 to 35% of previously synthesized proteins were secreted by the liver into the recirculating medium. Low temperature inhibited the...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Polycationic derivatives of ferritin containing primary amino groups (CFah) or tertiary amino groups (CFdmp) were potent platelet agonists inducing shape change, aggregation and secretion, but also agglutination in the presence of EDTA. Pretreatment of platelets with neuraminidase, PGE1, indomethacin, or creatine kina...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:In the eye lens, three connexins have been detected in epithelial cells and bow region/differentiating fiber cells, suggesting the possible formation of heteromeric gap junction channels. To study possible interactions between Cx56 and Cx43, we stably transfected a normal rat kidney cell line (NRK) that expresses Cx43...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00132
更新日期：2001-01-01 00:00:00
abstract：:During maize pollen embryogenesis, a range of multicellular structures are formed. Using different approaches, the "nature" of these structures has been determined in terms of their embryogenic potential. In situ molecular identification techniques for gene transcripts and products, and a novel cell tracking system in...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2005.02.002
更新日期：2005-07-01 00:00:00
abstract：:The organelles of the endocytic and autophagic pathways were studied in HeLa cells using immunoelectron microscopy and stereological techniques. In the absence of autophagic stimulation, characteristic particulate structures containing closely packed layers of membrane received the fluid-phase marker horseradish perox...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:Signal transduction elements, including protein kinase C, have been identified in mammalian spermatozoa. In order to evaluate the pattern of expression and the subcellular localization of nine different protein kinase C isoforms in the course of spermatogenesis, we utilized quantitative electron microscopy immunocytoc...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:The recently established in vitro assay of condensation-sorting of pancreatic enzymes to the zymogen granule membrane (ZGM) (Dartsch, H., R. Kleene, H. F. Kern: In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells. Eur. J. Cell Biol. 75, 211-222 (1998)) was used to study the invol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80009-0
更新日期：1999-02-01 00:00:00
abstract：:Monoclonal antibodies to the constitutive desmosomal glycoprotein desmoglein were characterized whose epitopes are located intracellularly, i.e., in the cytoplasmic portion of this molecule, and contribute to the structure of the desmosomal plaque. Using one of these antibodies (DG3.10), a peptide was isolated from a ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:Since various neuromuscular diseases are associated with abnormal glycosylation, it was of interest to determine whether this key post-translational modification is also altered in aged skeletal muscle. Lectins represent highly versatile carbohydrate-binding proteins that are routinely employed for the characterizatio...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2008.04.003
更新日期：2008-10-01 00:00:00
abstract：:Centaurin alpha-1 is a high-affinity PtdIns(3,4,5)P3-binding protein enriched in brain. Sequence analysis indicates centaurin alpha-1 contains two pleckstrin homology domains, ankyrin repeats and an Arf GAP homology domain, placing it in the AZAP family of phosphoinositide-regulated Arf GAPs. Other members of this fam...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00416
更新日期：2004-10-01 00:00:00
abstract：:Cytolocalization of stomatin, an integral membrane protein also called erythrocyte band 7.2b, was investigated in a human epithelial cell line in which the expression of this protein is up-regulated after treatment with interleukin-6 and dexamethasone. A monoclonal antibody against stomatin was used to perform immunof...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:RNA interference (RNAi) is a powerful tool for functional genetic studies in model organisms and mammalian cells. To facilitate rapid construction of gene knockdown constructs and RNAi libraries for known genes of mammalian cells, a new and simple strategy to produce small interfering RNA (siRNA) expression vectors wi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2005.10.009
更新日期：2006-05-01 00:00:00
abstract：:A great body of data increasingly point to the cell membrane as an important target for adriamycin (ADR). However, the exact mechanism by which ADR exerts its cytotoxic action through the interaction with the plasma membrane is still unknown. In this study, the interaction of ADR with red blood cells from healthy dono...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:Dictyosomes, Golgi vesicles, and plasma membranes were investigated after freeze-fracture in cells from growing root tips of cress (Lepidium sativum L.), that are distinguishable by different cellulose content of the cell wall, into (i) meristematic cells during early formation of the cell plate, (ii) statocytes of th...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:The central feature of fluid and electrolyte secretion by salivary acinar cells is transepithelial Cl- movement as a driving force for the secretion. However, little is known about the membrane localization and regulation by agonists of various anion channels. To characterize the anion transport and fluid secretion, w...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/S0171-9335(04)70021-7
更新日期：2000-03-01 00:00:00
abstract：:Filensin is a lens-specific intermediate filament protein, expressed in the lens fiber cells but not the lens epithelium. Using antibodies to filensin and the other lens intermediate filament proteins, vimentin and CP49, the codistribution of filensin with CP49 and independence of this network from the vimentin networ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:The aim of this study was to evaluate the influence of peroxisome proliferator-activated receptor γ (PPARγ) on self-renewal of mouse embryonic stem cells (mESCs) in the presence and absence of leukemia inhibitory factor (LIF). We demonstrated that in the presence of LIF, the activation of PPARγ by Rosiglitazone led to...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2013.03.003
更新日期：2013-04-01 00:00:00
abstract：:The entry of sporozoites of Theileria parva into bovine lymphoid cells in vitro was studied with the electron microscope. Endocytosis is completed in less than 10 min. No local mobilization of actin or other cytoskeletal elements is detected in the cytoplasm of the cell being invaded and no engulfing pseudopods are fo...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:The uptake mechanism of pertussis toxin (PT) in CHO and insulin-producing HIT-T15 cells was studied. By electron microscopy after direct labeling of the toxin with gold particles, PT was found to be taken up by receptor-mediated endocytosis. The presence of active pertussis toxin in the Golgi complex was shown by subc...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：:In recent years, several antagonists of alpha(v)beta3 have been used to develop therapeutic approaches to the treatment of melanoma neoplasia. We studied the effects of anti-alpha(v)-integrin-blocking antibodies on attached M21 melanoma cells, the cellular distribution of alpha(v)-integrin and the molecular organizati...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00067
更新日期：2000-07-01 00:00:00
abstract：:The simultaneous addition of alcian blue and tannic acid to human blood platelets during glutaraldehyde fixation leads to an excellent representation of coated membranes (CM). Coated membrane segments can be found (1) in plasmalemma, in vesicle system and lysosomal granules, or (2) as coated pits or coated vesicles. T...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:The lectins Griffonia simplicifolia I and Lycopersicon esculentum were used to assess the presence of endothelium-specific glycoproteins in the microvasculature of the rat myocardium, diaphragm and superficial cerebral cortex. Organs fixed by intravascular perfusion were processed to obtain semithin (0.5 micron) and t...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:Procaine-treated eggs can be penetrated by more than one spermatozoon. Supernumerary male pronuclei can fuse with the female one giving raise to multipolar spindles or remain isolated within the egg's cytoplasm forming their own spindle. In all types of multiple mitotic figures (asters and spindles) the distribution o...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:During endotoxic shock, the liver exerts an endotoxin (lipopolysaccharide, LPS) clearance function with the participation of both sinusoidal (mainly Kupffer and endothelial cells) and parenchymal cells. In order to determine the specificity and diversity of response of each liver cell type, the effect of Escherichia c...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
