Abstract：
:Rabbit antisera against homogeneous rat liver thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 1.6.4.5) were prepared and used for immunohistochemical analysis in adult rats. Immunoreactive thioredoxin and thioredoxin reductase were widely distributed in tissues and organs, but varied a lot between cell types. Generally, epithelial cells, neuronal cells and secretory cells, both exocrine and endocrine, showed high immunoreactivity whereas mesenchymal cells with exceptions showed low activity. Surface lining epithelial and keratinizing cells showed high activity. The immunofluorescence was localized in the cytoplasm of cells with enrichments at secretory granules, at the plasma membrane or in the subplasma membrane zone. Variations in secretory cells were seen related to feeding and starvation and to metabolic activity. The distribution of thioredoxin and thioredoxin reductase is compatible with function in thiol-disulfide interchange reaction related to protein synthesis, intracellular transport and different forms of secretion.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Rozell B,Hansson HA,Luthman M,Holmgren Asubject
Has Abstractpub_date
1985-07-01 00:00:00pages
79-86issue
1eissn
0171-9335issn
1618-1298journal_volume
38pub_type
杂志文章abstract：:Oocyte-thymocyte mouse cell hybrids were produced using polyethylene glycol (PEG) and examined at the ultrastructural level. Fusion was accomplished either before or after activation of metaphase II oocytes. In both experimental variants thymocyte nuclei undergo remodelling which comprises the following sequence of ev...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:The development of an efficient immune response depends on the capacity of antigen-specific lymphocytes to migrate into secondary lymphoid organs. The first step in the process of lymphocyte extravasation involves lymphocyte binding to the vascular endothelium. Although several adhesion receptors have been implicated ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:Involvement of CDK2 in glucocorticoid-mediated S-phase lengthening was analyzed in this work. Dexamethasone (DXM) treatment of PHA-stimulated lymphocytes induced a decrease in CDK2 mRNA expression without any change in mRNA stability. This glucocorticoid-induced decrease in CDK2 mRNA expression could be suppressed by ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00298
更新日期：2003-05-01 00:00:00
abstract：:Various drugs added to the culture medium of rabbit mammary gland were assayed for their capacity to affect the lactogenic and the mammogenic activities of prolactin. Three lysosomotropic agents NH4Cl, chloroquine and methylamine which were previously demonstrated to inhibit the degradation of the hormone-receptor com...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:It was previously shown that cultured mouse peritoneal macrophages ingest anionic derivatives of horseradish peroxidase (HRP) and ferritin by fluid-phase endocytosis and accumulate them in lysosomes. Cationic derivatives were taken up by adsorptive endocytosis and transported to lysosomes but subsequently appeared als...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:Dendritic cells (DC) are the most effective cells for antigen presentation in primary immune responses. Human cord blood CD34+ progenitors cultured in the presence of GM-CSF and TNF alpha generate a heterogeneous population of DC including Langerhans-like DC (LLDC) and monocytes. We describe here that IL-4 exerts diff...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80124-6
更新日期：1998-03-01 00:00:00
abstract：:The differences in the content of trace elements (Fe, Cu, Zn, Br, Rb and Sr) in phasic and tonic scallop adductor muscles have been revealed by using X-ray fluorescence analysis. The phasic (yellow) muscles have been found to be richer in Fe and Cu while more deficient in Zn and Br as compared to the tonic (white) mus...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:When resting human blood platelets are stimulated with thrombin, 50 to 60% of the G-actin polymerizes to F-actin within 60 seconds. The increase in F-actin is correlated with a corresponding decrease in the complex of G-actin with T beta 4. Within 5 seconds after stimulation, nucleation sites for pyrene actin polymeri...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:Recently, we have described the developmental expression of the small heat shock proteins (sHsps) Hsp27/HspB1 and alphaB-crystallin/HspB5 in different tissues of pigs from almost full-term foetuses to three years old adults (P. Tallot, J. F. Grongnet, J. C. David, Biol. Neonate, 83, 281-288, 2003). The data described ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00337
更新日期：2003-10-01 00:00:00
abstract：:Tetrahymena pyriformis GL is a suitable model to study the influence on phagocytotic activity of two membrane-stabilizing benzimidazole nitrogen mustard derivative, ZIMET 3164 and ZIMET 3393 (cytostasan). Treatment by both compounds causes a gradual inhibition of food vacuole formation dependent on the concentrations ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:Secretory granules of GH4C1 cells, a rat pituitary tumor cell line, are known to be induced by the treatment of estradiol (E2), insulin, and epidermal growth factor (EGF). We examined changes in the localization of cathepsins B, H, and L, lysosomal cysteine proteinases, in GH4C1 cells before and after hormonal treatme...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:The sister chromatid cohesion complex of Saccharomyces cerevisiae includes chromosomal ATPases Smc1p and Smc3p, the kleisin Mcd1p/Scc1p, and Irr1p/Scc3p, the least studied component. We have created an irr1-1 mutation (F658G substitution) which is lethal in the haploid and semi-dominant in the heterozygous diploid irr...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2008.05.002
更新日期：2008-10-01 00:00:00
abstract：:Morphological explanations for the effects of cytochalasin B (CB) on the digestive vacuole system of Paramecium caudatum were sought using electron-microscopic studies. Cytochalasin B (0.3 mM) essentially stopped vacuole release without stopping vacuole growth. Acidosome fusion with the new vacuoles was inhibited and ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:The serine/arginine rich proteins (SR proteins) are members of a family of RNA binding proteins involved in regulating various features of RNA metabolism, including pre-mRNA constitutive and alternative splicing. In humans, a total of 12 SR splicing factors (SRSFs) namely SRSF1-SRSF12 have been reported. SRSF3, the sm...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2020.151099
更新日期：2020-08-01 00:00:00
abstract：:Members of the transforming growth factor beta (TGF-beta) superfamily of signaling molecules are involved in the regulation of many developmental processes that involve the interaction between mesenchymal and epithelial tissues. Smad7 is a potent inhibitor of many members of the TGF-beta family, notably TGF-beta and a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2007.03.005
更新日期：2007-12-01 00:00:00
abstract：:In the present study the role of endothelin (ET) and its receptors (ETA-R and ETB-R) in cellular mechanisms underlying the resistance of astroglial cells to low oxygen level and development of hypoxia has been investigated. To define the influences of ET and its receptors on survival and on antigenic as well as morpho...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2004.12.030
更新日期：2005-06-01 00:00:00
abstract：:SNAREs are compartmentally specific membrane proteins required for intracellular membrane fusion. Homologues of the Saccharomyces cerevisiae protein Sec1p interact with, and are likely to be involved in regulation of, the syntaxin family of SNAREs. In yeast there are 7 functionally distinct syntaxins but only four cle...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/s0171-9335(98)80084-8
更新日期：1998-12-01 00:00:00
abstract：:In recent decades, research on peroxisome biogenesis has been particularly boosted since the role of these organelles in metabolism became unraveled. Indeed in plants, yeasts and fungi, peroxisomes play an important role in the adaptation of metabolism during developmental processes and/or altered environmental condit...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2010.04.001
更新日期：2010-09-01 00:00:00
abstract：:It was previously shown that avidin, a glycoprotein secreted in vivo by chicken oviduct, is produced by cultured transformed or damaged chicken embryo fibroblasts [27]. This report demonstrates synthesis and secretion of large amounts of avidin by macrophages isolated from chicken yolk sac. Avidin was secreted to the ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:The capacity of vascular endothelial cells to modulate their phenotype in response to changes in environmental conditions is one of the most important characteristics of this cell type. Since different growth factors may play an important signalling role in this adaptive process we have investigated the effect of endo...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00084
更新日期：2000-09-01 00:00:00
abstract：:RNA interference (RNAi) is a powerful tool for functional genetic studies in model organisms and mammalian cells. To facilitate rapid construction of gene knockdown constructs and RNAi libraries for known genes of mammalian cells, a new and simple strategy to produce small interfering RNA (siRNA) expression vectors wi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2005.10.009
更新日期：2006-05-01 00:00:00
abstract：:A great body of data increasingly point to the cell membrane as an important target for adriamycin (ADR). However, the exact mechanism by which ADR exerts its cytotoxic action through the interaction with the plasma membrane is still unknown. In this study, the interaction of ADR with red blood cells from healthy dono...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:The costae are cytoskeletal structures found in Trichomonadidae. Both the structural organization and composition of this organelle are still unknown. In the present work we have introduced a new methodology for the costa isolation. Using sucrose density-gradient centrifugation an enriched costa fraction was obtained....
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:SGLT1, an isoform of Na+-dependent glucose cotransporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine and the kidney, where it plays a pivotal role in the absorption and reabsorption of sugars, respectively. To search the domain responsible for the apical localization of ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00204
更新日期：2001-12-01 00:00:00
abstract：:Expression of the Cdx1 homeobox gene in epithelial intestinal cells promotes cellular growth and differentiation. Cdx1and the Pancreatitis Associated Protein I (PAP I) are concomitantly expressed in the epithelial cells of the lower part of the intestinal crypts. Because Cdx1 is a transcription factor and PAP I, in ot...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00148
更新日期：2001-02-01 00:00:00
abstract：:The hexameric ATPase p97/yeast Cdc48p has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. p97/Cdc48p contains two conserved consensus p34cdc2 kinase phosphorylation sites with...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80055-7
更新日期：1999-04-01 00:00:00
abstract：:The milk yeast Kluyveromyces lactis is an alternative model yeast to the well established Saccharomyces cerevisiae. The cell wall of these fungi consists of polysaccharides (i.e. long chains of β-1,3- and β-1,6-linked sugar chains and some chitin) and mannoproteins, both of which are continually adapted to environment...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2011.04.005
更新日期：2011-09-01 00:00:00
abstract：:It is generally believed that the induction of actin cytoskeleton rearrangements by extracellular stimuli results from the activation of guanine nucleotide exchange factors for the Rho GTPases. Here, we present evidence that the inactivation of RhoGAP (GTPase activating protein) activity is an equally effective means ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80019-3
更新日期：1999-08-01 00:00:00
abstract：:Herein we report that Gts1p fused with green-fluorescent protein (GFP) is localized in the cortical actin patch besides nuclei in yeast and the cortical Gts1p changed its position together with the patch depending on the cell-cycle phase, while nuclear Gts1p accumulated predominantly in the budding phase. Whereas Gts1...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2007.02.005
更新日期：2007-05-01 00:00:00
abstract：:Conventional fixation with glutaraldehyde fixatives at pH 7.4 did not preserve/promote thick filaments in Bufo smooth muscle. However, if pH was lowered to 6.0, thick filaments were present and addition of dimethyl sulfoxide (DMSO) led to a greater number of thick filaments. Stretch alone had little effect on the pres...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
