Abstract：
:The sister chromatid cohesion complex of Saccharomyces cerevisiae includes chromosomal ATPases Smc1p and Smc3p, the kleisin Mcd1p/Scc1p, and Irr1p/Scc3p, the least studied component. We have created an irr1-1 mutation (F658G substitution) which is lethal in the haploid and semi-dominant in the heterozygous diploid irr1-1/IRR1. The mutated Irr1-1 protein is present in the nucleus, its level is similar to that of wild-type Irr1p/Scc3p and it is able to interact with chromosomes. The irr1-1/IRR1 diploid exhibits mitotic and meiotic chromosome segregation defects, irregularities in mitotic divisions and is severely affected in meiosis. These defects are gene-dosage dependent, and experiments with synchronous cultures suggest that they may result from the malfunctioning of the spindle assembly checkpoint. The partial structure of Irr1p/Scc3p was predicted and the F658G substitution was found to induce marked changes in the general shape of the predicted protein. Nevertheless, the mutant protein retains its ability to interact with Scc1p, another component of the cohesin complex, as shown by coimmunoprecipitation.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Cena A,Kozłowska E,Płochocka D,Grynberg M,Ishikawa T,Fronk J,Kurlandzka Adoi
10.1016/j.ejcb.2008.05.002subject
Has Abstractpub_date
2008-10-01 00:00:00pages
831-44issue
10eissn
0171-9335issn
1618-1298pii
S0171-9335(08)00084-8journal_volume
87pub_type
杂志文章abstract：:When resting human blood platelets are stimulated with thrombin, 50 to 60% of the G-actin polymerizes to F-actin within 60 seconds. The increase in F-actin is correlated with a corresponding decrease in the complex of G-actin with T beta 4. Within 5 seconds after stimulation, nucleation sites for pyrene actin polymeri...
journal_title：European journal of cell biology
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更新日期：1993-08-01 00:00:00
abstract：:Vinculin, discovered in 1979 (Geiger, 1979), is an adapter protein with binding sites for more than 15 proteins. Biochemical and structural analyses have contributed to detailed knowledge about potential binding partners and the understanding of how their binding may be regulated. Despite all this information the mole...
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journal_title：European journal of cell biology
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更新日期：1989-12-01 00:00:00
abstract：:Using an exogenous kinase, nuclear ribonucleoprotein complexes with sedimentation coefficients greater than 100S were phosphorylated in vitro before and after treatment with increasing concentrations of NaC1. The phosphorylation pattern of the proteins before raising the NaC1 concentration shows a major group of label...
journal_title：European journal of cell biology
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更新日期：1981-12-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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更新日期：1979-10-01 00:00:00
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更新日期：1981-02-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1989-02-01 00:00:00
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更新日期：1982-08-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1987-06-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1994-02-01 00:00:00
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更新日期：1980-10-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1982-08-01 00:00:00
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