Abstract:
:Involvement of CDK2 in glucocorticoid-mediated S-phase lengthening was analyzed in this work. Dexamethasone (DXM) treatment of PHA-stimulated lymphocytes induced a decrease in CDK2 mRNA expression without any change in mRNA stability. This glucocorticoid-induced decrease in CDK2 mRNA expression could be suppressed by cycloheximide treatment. These results support the hypothesis of an indirect effect of DXM at the transcriptional level. Furthermore, CDK2 protein expression also decreased while the rate of protein synthesis and stability remained unchanged, suggesting a second post-translational level of regulation with the preferential degradation of a CDK2 protein stock fraction. The analysis of co-precipitated proteins showed that glucocorticoids induced modifications of protein complex composition. We found i) a preservation of the linkage capability of CDK2 for cyclin E and A, ii) a relative increase in p27kip1 linkage, and iii) a decrease in p21waf1 complexed with CDK2. As a consequence of CDK2 decrease and modifications of protein complex composition, pRb and histone H1 kinase activity of CDK2 was profoundly decreased. All these results pinpoint the role of CDK2 in glucocorticoid-induced S-phase lengthening and the potential activator role of p21waf1 for CDK2 in human lymphocytes.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Peiretti A,Baghdassarian N,Gerland LM,Ffrench P,Bryon PA,Magaud JP,Ffrench Mdoi
10.1078/0171-9335-00298subject
Has Abstractpub_date
2003-05-01 00:00:00pages
253-61issue
5eissn
0171-9335issn
1618-1298pii
S0171-9335(04)70296-4journal_volume
82pub_type
杂志文章abstract::Application of carbon tetrachloride produced a progressive proliferation of tight junctions in the rat liver. This system proved to be rapid and highly reproducable and affords the opportunity for tracing the fate of tight junctions in freeze-fracture replicas, facilitating investigations on their formation and functi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::The signal transduction pathways of chondrocyte area expansion and migration under periodic mechanical stress remain a matter of debate. We explore this question by performing cell culture experiments in our self-developed periodic stress field and perfusion culture system. Under periodic mechanical stress, we find th...
journal_title:European journal of cell biology
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doi:10.1016/j.ejcb.2010.04.005
更新日期:2010-09-01 00:00:00
abstract::In the literature the question of whether a system structurally and functionally related to the barrier function of the tight junctions (TJs) of polarized epithelia exists in the epidermis has been and still is controversially discussed. We have systematically addressed this question in a study of the granular layer o...
journal_title:European journal of cell biology
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doi:10.1078/0171-9335-00434
更新日期:2004-12-01 00:00:00
abstract::The method for electron microscopy of the Ag-staining of NOR-specific proteins was adapted to tissue sections from solid organs. The distribution of the Ag-staining proteins in rat liver nucleoli after complete block of transcription caused by D-galactosamine was investigated. In control animals, the Ag-staining prote...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::Stereo immunofluorescence microscopy avoids the problem of juxtaposition of structures often encountered in normal fluorescence microscopy. The procedure has been used in conjunction with antibodies against microfilament associated proteins to reveal the arrangement of microfilaments in a rat mammary cell line both in...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::The nuclear lamins are karyophilic proteins located at the nucleoplasmic surface of the inner nuclear membrane. We have constructed mutants immediately N-terminal to the nuclear localization signal of human lamin A to identify sites regulating the nuclear transport of the protein. Using an in vitro transport assay, we...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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abstract::Taking advantage of monoclonal antibodies raised against chick hypertrophic chondrocytes (BD5, LA5, AD2) and osteoblasts (SB1, SB2, SB3, SB5), we have investigated expression of differentiation stage-specific antigens by further differentiating hypertrophic chondrocytes. Expression of all antigens was developmentally ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-06-01 00:00:00
abstract::The transport of the chitin synthase III, Chs3p, to the plasma membrane is temporally and spatially regulated. Chs3p is delivered to the plasma membrane at the beginning of the cell cycle, forming chitin rings, and at the end of the cell cycle, forming the primary septum. During the rest of the cell cycle, it is maint...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.10.006
更新日期:2006-02-01 00:00:00
abstract::We have reported previously that the human promyelocytic leukemia cell line HL-60, in its undifferentiated state, is devoid of cytoplasmic intermediate filament proteins and nuclear lamins A and C, but does express lamin B. Using immunofluorescence and immunoblotting techniques, we have further investigated the expres...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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更新日期:2011-10-01 00:00:00
abstract::Sarcoplasmic reticulum fragments capable of accumulating calcium were isolated from rat skeletal muscle by differential and sucrose gradient centrifugation. The ability of these fragments to accumulate calcium was impaired by adding 2,2-bis-(p-chlorophenyl)-1,1,1-trichloroethane (DDT) to the assay medium at concentrat...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::The loss of epidermal growth factor (EGF) binding activity on cultured murine 3T3 cells exposed to EGF (EGF receptor down regulation) was determined in colchicine treated cells, cytochalasin B treated cells, and untreated cells. Neither colchicine nor cytochalasin B altered the affinity of the receptor for EGF, but co...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract::The spatial and temporal distribution as well as ultrastructural and biochemical characteristics of apoptotic and mitotic cells during human eye development were investigated in 14 human conceptuses of 4-9 postovulatory weeks, using electron and light microscopy. In the 5th developmental week, apoptotic and mitotic ce...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00328
更新日期:2003-08-01 00:00:00
abstract::Rabbit antisera against homogeneous rat liver thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 1.6.4.5) were prepared and used for immunohistochemical analysis in adult rats. Immunoreactive thioredoxin and thioredoxin reductase were widely distributed in tissues and organs, but va...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::D-amino acid oxidase is expressed to a high level in the yeast Rhodotorula gracilis (0.3% of total cell protein) through induction by D-alanine in a defined growth medium. Monospecific polyclonal antibodies against pure enzyme were obtained. Western blot analysis showed that the enzyme is synthesized as the mature pol...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::The giant syncytium of Physarum plasmodia possesses a complex cytoplasmic microtubule network except during the occurrence of the intranuclear mitosis. In early prophase stages, intranuclear spindles assemble concomitantly as the cytoplasmic microtubule network disassembles. No cytoplasmic microtubules are present in ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Myeloid hematopoietic precursor cells are induced to differentiate by the macrophage and granulocyte differentiation-inducing protein MGI-2 (DF). This differentiation-inducing protein bound to double-stranded but not to single-stranded mammalian DNA. The bound MGI-2 was not eluted by high salt, but was eluted by sodiu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::The large protein bodies of the storage parenchyma cells of mung bean (Vigna radiata) cotyledons contain vesicles measuring 0.2 to 2.0 mum in diameter. The vesicles contain ribosomes, ribosomes, membranous elements which may be derived from the endoplasmic reticulum and occasionally Golgi bodies and mitochondria. The ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::A set of monoclonal antibodies directed against various gastric markers was produced in order to study the developmental expression of the gastric mucosa. A previously described monoclonal antibody, mab 146.14, labeled the proton pump (H+, K+) ATPase specifically located in gastric parietal cells. Mabs 15.1 and 121.17...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
abstract::Phagosomes with pathogenic mycobacteria, or with hydrophobic polystyrene beads of 1 micron in size, do not mature, but remain fusogenic towards early endosomes and do not fuse with lysosomes (de Chastellier et al. Eur. J. Cell Biol. 68, 167-182 (1995)). Both types of phagocytic particles display a close apposition to ...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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doi:10.1016/S0171-9335(99)80093-4
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
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