CDK2 is involved in the S-phase lengthening induced by glucocorticoids in normal human lymphocytes.

Abstract:

:Involvement of CDK2 in glucocorticoid-mediated S-phase lengthening was analyzed in this work. Dexamethasone (DXM) treatment of PHA-stimulated lymphocytes induced a decrease in CDK2 mRNA expression without any change in mRNA stability. This glucocorticoid-induced decrease in CDK2 mRNA expression could be suppressed by cycloheximide treatment. These results support the hypothesis of an indirect effect of DXM at the transcriptional level. Furthermore, CDK2 protein expression also decreased while the rate of protein synthesis and stability remained unchanged, suggesting a second post-translational level of regulation with the preferential degradation of a CDK2 protein stock fraction. The analysis of co-precipitated proteins showed that glucocorticoids induced modifications of protein complex composition. We found i) a preservation of the linkage capability of CDK2 for cyclin E and A, ii) a relative increase in p27kip1 linkage, and iii) a decrease in p21waf1 complexed with CDK2. As a consequence of CDK2 decrease and modifications of protein complex composition, pRb and histone H1 kinase activity of CDK2 was profoundly decreased. All these results pinpoint the role of CDK2 in glucocorticoid-induced S-phase lengthening and the potential activator role of p21waf1 for CDK2 in human lymphocytes.

journal_name

Eur J Cell Biol

authors

Peiretti A,Baghdassarian N,Gerland LM,Ffrench P,Bryon PA,Magaud JP,Ffrench M

doi

10.1078/0171-9335-00298

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

253-61

issue

5

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(04)70296-4

journal_volume

82

pub_type

杂志文章
  • Plant microtubule-associated proteins (MAPs) affect microtubule nucleation and growth at plant nuclei and mammalian centrosomes.

    abstract::In this study, we investigated the effect of plant microtubule-associated proteins (MAPs) on microtubule nucleation and growth in vitro. Since it has recently been demonstrated that plant nuclear surface acts as a microtubule-organizing center (MTOC), we tested the effects of plant MAPs using a nucleus-mediated microt...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Stoppin V,Lambert AM,Vantard M

    更新日期:1996-01-01 00:00:00

  • Filaments associated with the endoplasmic reticulum in the streaming cytoplasm of Chara corallina.

    abstract::Perfused Chara cells capable of resuming ATP-dependent cytoplasmic streaming in low free Ca++ solutions have been examined by electron microscopy for myosin-like filaments. Filaments 44 nm in diameter and up to 3 micron in length have been found associated with the endoplasmic reticulum that along with mitochondria, m...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Williamson RE

    更新日期:1979-12-01 00:00:00

  • Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules.

    abstract::Monoclonal antibodies to the constitutive desmosomal glycoprotein desmoglein were characterized whose epitopes are located intracellularly, i.e., in the cytoplasmic portion of this molecule, and contribute to the structure of the desmosomal plaque. Using one of these antibodies (DG3.10), a peptide was isolated from a ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Koch PJ,Walsh MJ,Schmelz M,Goldschmidt MD,Zimbelmann R,Franke WW

    更新日期:1990-10-01 00:00:00

  • Coordinated regulation of procollagens I and III and their post-translational enzymes by dissipation of mechanical tension in human dermal fibroblasts.

    abstract::Mechanical tension governs fibroblast proliferation and survival and the homeostasis of the extracellular matrix to adapt its resistance to the mechanical requirements of the organs. To consolidate this view, we analysed the effect of tension release on the expression of molecules involved in the architecture and stab...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00181

    authors: Lambert CA,Colige AC,Lapière CM,Nusgens BV

    更新日期:2001-07-01 00:00:00

  • Visualization of the secretory process involved in Ca2+-activated fluid secretion from rat submandibular glands using the fluorescent dye, calcein.

    abstract::The central feature of fluid and electrolyte secretion by salivary acinar cells is transepithelial Cl- movement as a driving force for the secretion. However, little is known about the membrane localization and regulation by agonists of various anion channels. To characterize the anion transport and fluid secretion, w...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/S0171-9335(04)70021-7

    authors: Sugita M,Hirono C,Tanaka S,Nakahari T,Imai Y,Kanno Y,Shiba Y

    更新日期:2000-03-01 00:00:00

  • Immunohistochemical localization of thioredoxin and thioredoxin reductase in adult rats.

    abstract::Rabbit antisera against homogeneous rat liver thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 1.6.4.5) were prepared and used for immunohistochemical analysis in adult rats. Immunoreactive thioredoxin and thioredoxin reductase were widely distributed in tissues and organs, but va...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Rozell B,Hansson HA,Luthman M,Holmgren A

    更新日期:1985-07-01 00:00:00

  • Electron microscopic localization of silver staining NOR-proteins in rat liver nucleoli upon D-galactosamine block of transcription.

    abstract::The method for electron microscopy of the Ag-staining of NOR-specific proteins was adapted to tissue sections from solid organs. The distribution of the Ag-staining proteins in rat liver nucleoli after complete block of transcription caused by D-galactosamine was investigated. In control animals, the Ag-staining prote...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Dimova RN,Markov DV,Gajdardjieva KC,Dabeva MD,Hadjiolov AA

    更新日期:1982-10-01 00:00:00

  • The minimal ESCRT machinery of Giardia lamblia has altered inter-subunit interactions within the ESCRT-II and ESCRT-III complexes.

    abstract::The ESCRT pathway functions at different subcellular membranes to induce their negative curvature, and it has been largely characterized in model eukaryotes belonging to Opisthokonta. But searches of the genomes of many nonopisthokonts belonging to various supergroups indicate that some of them may harbour fewer ESCRT...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2017.11.004

    authors: Saha N,Dutta S,Datta SP,Sarkar S

    更新日期:2018-01-01 00:00:00

  • Molecular dissection of ODF2/Cenexin revealed a short stretch of amino acids necessary for targeting to the centrosome and the primary cilium.

    abstract::The outer dense fiber protein ODF2 is the major component of the sperm tail cytoskeleton and a critical component of the mature centriole of the centrosome. Centriole maturation involves the formation of appendages and the recruitment of ODF2/Cenexin. ODF2 and Cenexin are alternative splice variants that differ in a s...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2007.10.004

    authors: Hüber D,Geisler S,Monecke S,Hoyer-Fender S

    更新日期:2008-03-01 00:00:00

  • Mitochondrial reactive oxygen species are involved in chemoattractant-induced oxidative burst and degranulation of human neutrophils in vitro.

    abstract::Activation of neutrophils is accompanied by the oxidative burst, exocytosis of various granule types (degranulation) and a delay in spontaneous apoptosis. The major source of reactive oxygen species (ROS) in human neutrophils is NADPH oxidase (NOX2), however, other sources of ROS also exist. Although the function of R...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2017.03.003

    authors: Vorobjeva N,Prikhodko A,Galkin I,Pletjushkina O,Zinovkin R,Sud'ina G,Chernyak B,Pinegin B

    更新日期:2017-05-01 00:00:00

  • Skeletal muscle-specific variant of nascent polypeptide associated complex alpha (skNAC): implications for a specific role in mammalian myoblast differentiation.

    abstract::NAC (nascent polypeptide associated complex) is a heterodimer consisting of an α and a β subunit. skNAC (skeletal and heart muscle-specific form of αNAC) is a variant of αNAC, which is induced in muscle differentiation and regeneration. We show here that skNAC expression is regulated by p38 MAPK, which plays a crucial...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2011.10.004

    authors: Berger F,Berkholz J,Breustedt T,Ploen D,Munz B

    更新日期:2012-02-01 00:00:00

  • Dual effects of peroxisome proliferator-activated receptor γ on embryonic stem cell self-renewal in presence and absence of leukemia inhibitory factor.

    abstract::The aim of this study was to evaluate the influence of peroxisome proliferator-activated receptor γ (PPARγ) on self-renewal of mouse embryonic stem cells (mESCs) in the presence and absence of leukemia inhibitory factor (LIF). We demonstrated that in the presence of LIF, the activation of PPARγ by Rosiglitazone led to...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2013.03.003

    authors: Peymani M,Ghoochani A,Ghaedi K,Karamali F,Karbalaie K,Kiani-Esfahani A,Rabiee F,Nasr-Esfahani MH,Baharvand H

    更新日期:2013-04-01 00:00:00

  • Activity of Kex2 dibasic endoprotease is localized throughout the secretory pathway in Saccharomyces cerevisiae. An ultracytochemical study.

    abstract::The sites of calcium-dependent dibasic endoprotease (Kex2; yscF) activity have been ultracytochemically localized in exponential cultures of haploid (alpha) wild-type strain of Saccharomyces cerevisiae, in its pep4-3 mutant and in the kex2 mutant. The gently prefixed cells were thoroughly washed and incubated in a buf...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Vorísek J

    更新日期:1994-02-01 00:00:00

  • Identification of a minus end-specific microtubule-associated protein located at the mitotic poles in cultured mammalian cells.

    abstract::A mitosis-specific centrosomal component was studied with a human autoantibody, SP-H, which immunostained mitotic poles and interphase nuclei, and a single polypeptide with an apparent molecular mass of 200 to 230 kDa in various lines of cultured cells. Early mitotic PtK1 cells treated with 10 micrograms/ml taxol cont...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Maekawa T,Leslie R,Kuriyama R

    更新日期:1991-04-01 00:00:00

  • Is the sensitivity of cells for FGF-1 and FGF-2 regulated by cell surface heparan sulfate proteoglycans?

    abstract::We have examined the importance of cell surface heparan sulfate proteoglycans (HSPG) in fibroblast growth factor (FGF) signaling. 3T3 cells grown under conventional conditions were much more sensitive to FGF-2 compared to FGF-1. However, cells were equally sensitive to FGF-1 and FGF-2 using conditions which reduced th...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Zhou FY,Owens RT,Hermonen J,Jalkanen M,Höök M

    更新日期:1997-06-01 00:00:00

  • Isolation of human monocytes according to volume and density.

    abstract::Human peripheral blood monocytes were obtained in very pure preparations by using buoyant density gradient centrifugation followed by velocity sedimentation. We introduced several modifications in the experimental procedure in order to take advantage of the minimal differences in cell density and size between monocyte...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Sitar G,Mascheretti P,Pesce G,Fornasari P,Peveri G

    更新日期:1986-04-01 00:00:00

  • Structure, synthesis, and orientation of microfibrils. IX. A freeze-fracture investigation of the Oocystis plasma membrane after inhibitor treatments.

    abstract::Cells of Oocystis solitaria after treatment with inhibitors of a) microfibril orientation b) microfibril synthesis, and c) microfibril crystallization have been investigated with the freeze-fracturing technique. Changes in morphology of the E-face of the plasma membrane as a result of these treatments have been record...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Robinson DG,Quader H

    更新日期:1981-10-01 00:00:00

  • Adhesions ring: a structural comparison between podosomes and the immune synapse.

    abstract::Podosomes and the immune synapse are integrin-mediated adhesive structures that share a common ring-like morphology. Both podosomes and immune synapses have a central core surrounded by a peripheral ring containing talin, vinculin and paxillin. Recent progress suggests significant parallels between the regulatory mech...

    journal_title:European journal of cell biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ejcb.2008.01.011

    authors: Wernimont SA,Cortesio CL,Simonson WT,Huttenlocher A

    更新日期:2008-09-01 00:00:00

  • Yeast dynamin Vps1 associates with clathrin to facilitate vesicular trafficking and controls Golgi homeostasis.

    abstract::The yeast dynamin Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains u...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2017.02.004

    authors: Goud Gadila SK,Williams M,Saimani U,Delgado Cruz M,Makaraci P,Woodman S,Short JC,McDermott H,Kim K

    更新日期:2017-03-01 00:00:00

  • Functional downregulation of the E-cadherin/catenin complex leads to loss of contact inhibition of motility and of mitochondrial activity, but not of growth in confluent epithelial cell cultures.

    abstract::When epithelial cells reach confluency in vitro, a number of energy-requiring activities such as growth and motility are contact-inhibited. We investigated the possible role of the E-cadherin/catenin complex, which acts as an invasion suppressor, in contact inhibition. Three strategies for modulation of the complex we...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Bracke ME,Depypere H,Labit C,Van Marck V,Vennekens K,Vermeulen SJ,Maelfait I,Philippé J,Serreyn R,Mareel MM

    更新日期:1997-12-01 00:00:00

  • A caudal-type homeobox gene activity associated with the development and regeneration of the liver.

    abstract::Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Doll U,Niessing J

    更新日期:1996-07-01 00:00:00

  • Multicellular structures developing during maize microspore culture express endosperm and embryo-specific genes and show different embryogenic potentialities.

    abstract::During maize pollen embryogenesis, a range of multicellular structures are formed. Using different approaches, the "nature" of these structures has been determined in terms of their embryogenic potential. In situ molecular identification techniques for gene transcripts and products, and a novel cell tracking system in...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2005.02.002

    authors: Massonneau A,Coronado MJ,Audran A,Bagniewska A,Mòl R,Testillano PS,Goralski G,Dumas C,Risueño MC,Matthys-Rochon E

    更新日期:2005-07-01 00:00:00

  • Heteromeric connexons formed by the lens connexins, connexin43 and connexin56.

    abstract::In the eye lens, three connexins have been detected in epithelial cells and bow region/differentiating fiber cells, suggesting the possible formation of heteromeric gap junction channels. To study possible interactions between Cx56 and Cx43, we stably transfected a normal rat kidney cell line (NRK) that expresses Cx43...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00132

    authors: Berthoud VM,Montegna EA,Atal N,Aithal NH,Brink PR,Beyer EC

    更新日期:2001-01-01 00:00:00

  • Absence of fodrin (spectrin-like protein) under the pseudopod membrane in stimulated thyroid cells.

    abstract::A fodrin-like protein purified from porcine thyroid cells and characterized by its properties identical to those of pig brain spectrin (F. Regnouf et al., Eur. J. Biochem. 153, 313-319 (1985)) has been localized by immunofluorescence and electron immunocytochemistry in porcine and rat thyroid. Fodrin-like polypeptides...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Gabrion JB,Barrière H,Nguyen Than Dao B,Chambard M,Mauchamp J,Regnouf F,Pradel LA

    更新日期:1990-08-01 00:00:00

  • An 18-bp element in the 5' untranslated region of the Drosophila beta 2 tubulin mRNA regulates the mRNA level during postmeiotic stages of spermatogenesis.

    abstract::During Drosophila spermatogenesis transcriptional activity is mainly restricted to premeiotic stages. Translation during sperm morphogenesis, however, proceeds for several days, requiring a high stability for mRNAs translated postmeiotically. We studied expression of the Drosophila beta 2 tubulin gene, which is expres...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Michiels F,Buttgereit D,Renkawitz-Pohl R

    更新日期:1993-10-01 00:00:00

  • Mitochondrial matrix localization of a protein altered in mutants resistant to the microtubule inhibitor podophyllotoxin.

    abstract::Specific antibodies to a protein designated P1 (Mr approximately equal to 63,000), which is specifically altered in mutants resistant to the microtubule inhibitor podophyllotoxin, bind to mitochondria in cells of various vertebrate and invertebrate species (Eur. J. Cell Biol. 44, 278-285 (1987); Can. J. Biochem. Cell ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Gupta RS,Austin RC

    更新日期:1987-12-01 00:00:00

  • Studies on megakaryocytes: isolation from rat and guinea pig and incorporation of 5-hydroxytryptamine.

    abstract::A new, two step method is described for the isolation of megakaryocytes from bone marrow. By means of centrifugation in a PercollR density gradient megakaryocytes can be enriched by factor 68 or 24 starting from rat or guinea pig bone marrow, respectively. The purity of the megakaryocytes can be improved ca. 4 fold if...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Seitz R,Wesemann W

    更新日期:1980-06-01 00:00:00

  • The multi-step process of human skin carcinogenesis: a role for p53, cyclin D1, hTERT, p16, and TSP-1.

    abstract::As proposed by Hanahan and Weinberg (2000. Cell 100, 57-70) carcinogenesis requires crucial events such as (i) genomic instability, (ii) cell cycle deregulation, (iii) induction of a telomere length maintenance mechanism, and (iv) an angiogenic switch. By comparing the expression of p53, cyclin D1, p16, hTERT, and TSP...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2006.11.002

    authors: Burnworth B,Arendt S,Muffler S,Steinkraus V,Bröcker EB,Birek C,Hartschuh W,Jauch A,Boukamp P

    更新日期:2007-12-01 00:00:00

  • Uptake and apparent digestion of cytoplasmic organelles by protein bodies (protein storage vacuoles) in mung bean cotyledons.

    abstract::The large protein bodies of the storage parenchyma cells of mung bean (Vigna radiata) cotyledons contain vesicles measuring 0.2 to 2.0 mum in diameter. The vesicles contain ribosomes, ribosomes, membranous elements which may be derived from the endoplasmic reticulum and occasionally Golgi bodies and mitochondria. The ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Herman EM,Baumgartner B,Chrispeels MJ

    更新日期:1981-06-01 00:00:00

  • Ultrastructure of the purified and reconstituted Na/K-ATPase of the avian salt gland.

    abstract::Na/K-ATPase of salt-stressed salt glands of the domestic duck (Anas platyrhynchos) was purified in membrane-bound form by incubation of the microsomal fraction with sodium dodecylsulphate and ATP followed by discontinuous sucrose gradient centrifugation. Gel electrophoresis of the purified plasma membrane preparation ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Gassner D,Komnick H

    更新日期:1983-01-01 00:00:00