Mitochondrial reactive oxygen species are involved in chemoattractant-induced oxidative burst and degranulation of human neutrophils in vitro.

Abstract:

:Activation of neutrophils is accompanied by the oxidative burst, exocytosis of various granule types (degranulation) and a delay in spontaneous apoptosis. The major source of reactive oxygen species (ROS) in human neutrophils is NADPH oxidase (NOX2), however, other sources of ROS also exist. Although the function of ROS is mainly defensive, they can also play a regulatory role in cell signaling. However, the contribution of various sources of ROS in these processes is not clear. We investigated a possible role of mitochondria-derived ROS (mtROS) in the regulation of neutrophil activation induced by chemoattractant fMLP in vitro. Using the mitochondria-targeted antioxidant SkQ1, we demonstrated that mtROS are implicated in the oxidative burst caused by NOX2 activation as well as in the exocytosis of primary (azurophil) and secondary (specific) granules. Scavenging of mtROS with SkQ1 slightly accelerated spontaneous apoptosis and significantly stimulated apoptosis of fMLP-activated neutrophils. These data indicate that mtROS play a critical role in signal transduction that mediates the major neutrophil functional responses in the process of activation.

journal_name

Eur J Cell Biol

authors

Vorobjeva N,Prikhodko A,Galkin I,Pletjushkina O,Zinovkin R,Sud'ina G,Chernyak B,Pinegin B

doi

10.1016/j.ejcb.2017.03.003

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

254-265

issue

3

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(16)30149-2

journal_volume

96

pub_type

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