Abstract:
:Activation of neutrophils is accompanied by the oxidative burst, exocytosis of various granule types (degranulation) and a delay in spontaneous apoptosis. The major source of reactive oxygen species (ROS) in human neutrophils is NADPH oxidase (NOX2), however, other sources of ROS also exist. Although the function of ROS is mainly defensive, they can also play a regulatory role in cell signaling. However, the contribution of various sources of ROS in these processes is not clear. We investigated a possible role of mitochondria-derived ROS (mtROS) in the regulation of neutrophil activation induced by chemoattractant fMLP in vitro. Using the mitochondria-targeted antioxidant SkQ1, we demonstrated that mtROS are implicated in the oxidative burst caused by NOX2 activation as well as in the exocytosis of primary (azurophil) and secondary (specific) granules. Scavenging of mtROS with SkQ1 slightly accelerated spontaneous apoptosis and significantly stimulated apoptosis of fMLP-activated neutrophils. These data indicate that mtROS play a critical role in signal transduction that mediates the major neutrophil functional responses in the process of activation.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Vorobjeva N,Prikhodko A,Galkin I,Pletjushkina O,Zinovkin R,Sud'ina G,Chernyak B,Pinegin Bdoi
10.1016/j.ejcb.2017.03.003subject
Has Abstractpub_date
2017-05-01 00:00:00pages
254-265issue
3eissn
0171-9335issn
1618-1298pii
S0171-9335(16)30149-2journal_volume
96pub_type
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journal_title:European journal of cell biology
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abstract::The sister chromatid cohesion complex of Saccharomyces cerevisiae includes chromosomal ATPases Smc1p and Smc3p, the kleisin Mcd1p/Scc1p, and Irr1p/Scc3p, the least studied component. We have created an irr1-1 mutation (F658G substitution) which is lethal in the haploid and semi-dominant in the heterozygous diploid irr...
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doi:10.1016/j.ejcb.2008.05.002
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
abstract::High-speed centrifugation supernatants from slime mould plasmodia show considerable activities to inhibit the polymerization of actin as revealed by viscosity measurements. By following increasing inhibitory activities an actin modulating protein (AM-protein) has been isolated and purified which affects the polymer st...
journal_title:European journal of cell biology
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doi:
更新日期:1981-02-01 00:00:00
abstract::The spatial and temporal distribution as well as ultrastructural and biochemical characteristics of apoptotic and mitotic cells during human eye development were investigated in 14 human conceptuses of 4-9 postovulatory weeks, using electron and light microscopy. In the 5th developmental week, apoptotic and mitotic ce...
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Involvement of CDK2 in glucocorticoid-mediated S-phase lengthening was analyzed in this work. Dexamethasone (DXM) treatment of PHA-stimulated lymphocytes induced a decrease in CDK2 mRNA expression without any change in mRNA stability. This glucocorticoid-induced decrease in CDK2 mRNA expression could be suppressed by ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00298
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
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doi:
更新日期:1983-05-01 00:00:00
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doi:
更新日期:1984-01-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
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更新日期:2007-06-01 00:00:00
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journal_title:European journal of cell biology
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doi:
更新日期:1981-08-01 00:00:00
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doi:
更新日期:1983-07-01 00:00:00
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更新日期:1981-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
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doi:
更新日期:1990-12-01 00:00:00