Abstract:
:The subcellular distribution of blood group A gene specified alpha 1,3N-acetylgalactosaminyltransferase and its product was studied in human intestinal goblet cells by immunoelectron microscopy. The O-glycosylation step yielding blood group A-active glycoconjugates occurred in the trans region of the Golgi apparatus as indicated by the presence of immunolabel for both antigens. In the Golgi apparatus, immunoreactive alpha 1,3N-acetylgalactosaminyltransferase was detectable in trans cisternae and in the trans-tubular network which was found to be continuous with the cisternal stack and exhibited acid phosphatase activity. This demonstrates that in intestinal goblet cells (i) the trans-tubular network does not constitute a compartment distinct from trans cisternae, and (ii) structures corresponding to GERL are structurally and functionally part of the Golgi apparatus. In addition to immunolabel for transferase at the inner surface of the cisternal membranes, luminally located immunolabel indicating the presence of free, not membrane-associated transferase became first detectable in the trans-tubular network and early forming mucus droplets contained therein. Further, the content of mature mucus droplets as well as the extracellular mucus layer were labeled. Absence of immunolabel in blood group 0 subjects lacking the blood group A gene specified transferase and the apparent non-reactivity of the antibodies with carbohydrate epitopes indicates that free alpha 1,3N-acetylgalactosaminyltransferase is present in mucus droplets and becomes secreted by intestinal goblet cells.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Roth J,Greenwell P,Watkins WMsubject
Has Abstractpub_date
1988-04-01 00:00:00pages
105-12issue
1eissn
0171-9335issn
1618-1298journal_volume
46pub_type
杂志文章abstract::Despite its frequent inactivation in human breast cancers, the role of p21(Cip1) (p21) in morphological plasticity of normal mammary epithelial cells is still poorly understood. To address this question, we have investigated the consequences of p21 silencing in two-dimensional (2D) morphogenesis of untransformed human...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2011.03.002
更新日期:2011-08-01 00:00:00
abstract::The transport of the chitin synthase III, Chs3p, to the plasma membrane is temporally and spatially regulated. Chs3p is delivered to the plasma membrane at the beginning of the cell cycle, forming chitin rings, and at the end of the cell cycle, forming the primary septum. During the rest of the cell cycle, it is maint...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.10.006
更新日期:2006-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::An increase in light intensity induces a depolarization in retinal ON-bipolar cells via a reduced glutamate release from presynaptic photoreceptor cells. The underlying transduction cascade in the dendritic tips of ON-bipolar cells involves mGluR6 glutamate receptors signaling to TRPM1 proteins that are an indispensab...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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更新日期:2015-07-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::By double indirect immunofluorescence and interference electron microscopy, we have observed the effect of microtubule disruption by antimitotic drugs and coldness treatment on the distribution of adhesion sites and of the three cytoskeletal proteins, vinculin, alpha-actinin, and actin in normal rat cells and in rat c...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
abstract::The giant syncytium of Physarum plasmodia possesses a complex cytoplasmic microtubule network except during the occurrence of the intranuclear mitosis. In early prophase stages, intranuclear spindles assemble concomitantly as the cytoplasmic microtubule network disassembles. No cytoplasmic microtubules are present in ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::When resting human blood platelets are stimulated with thrombin, 50 to 60% of the G-actin polymerizes to F-actin within 60 seconds. The increase in F-actin is correlated with a corresponding decrease in the complex of G-actin with T beta 4. Within 5 seconds after stimulation, nucleation sites for pyrene actin polymeri...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::Intact follicles as well as defolliculated oocytes of the mouse were studied by freeze-fracture electron microscopy. In intact follicles the oocyte plasma membrane shows two prominent types of intra-membrane particle array:gap junctions and yet undescribed rhombic particle arrays. The gap junctions vary in size (from ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Proteins that are concentrated in specific compartments of the endomembrane system in order to exert their organelle-specific function must possess specific localization signals that prevent their transport to distal regions of the exocytic pathway. Some resident proteins of the endoplasmic reticulum (ER) that are kno...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70025-4
更新日期:2000-04-01 00:00:00
abstract::In the sea urchin embryo there are at least two cell adhesion molecules related to mammalian cadherins, one of them, similar to E-cadherin, is expressed in embryos at very early developmental stages, the second appears at the blastula stage (G. Ghersi et al. Mech. Dev. 41, 47-55 (1993)). We show here that when sea urc...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::PinX1 was originally identified as a Pin2/TRF1-interacting protein that suppresses telomerase activity via its telomerase inhibitor domain (TID) and regulates the nucleolar localization of TRF1 in telomerase-positive cells. In addition to its telomeric localization, PinX1 can be found in the nucleoli of human cells. O...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2010.05.005
更新日期:2010-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
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journal_title:European journal of cell biology
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doi:
更新日期:1992-04-01 00:00:00
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journal_title:European journal of cell biology
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doi:
更新日期:1982-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::In flowering plants, gametes are formed in specialized haploid structures, termed gametophytes. The female gametophyte is a few-celled structure that integrates such diverse functions as pollen tube attraction, sperm cell release, gamete fusion and seed initiation. These processes are realized by distinct cell types, ...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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更新日期:2010-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2004.12.025
更新日期:2005-04-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1999-09-01 00:00:00