Abstract:
:Interleukin-1 beta (Il-1 beta) and interleukin-1 alpha (Il-1 alpha) were shown to act as motility factors for the human breast carcinoma cell lines SK-BR-3 and ZR-75-1 in vitro. Both cytokines induced transition from the stationary to the motile phenotype (spreading). Il-1 beta stimulated translocation, shape change and random migration (chemokinesis) of SK-BR-3 cells as demonstrated by time-lapse video recordings and by a modified Boyden chamber assay. Interleukin-6 (Il-6) stimulated spreading of the SK-BR-3 cells; an additive effect with Il-1 beta on spreading and fast plasma membrane movements was evidenced. In the SK-BR-3 cell line, the signal transduction of Il-1 beta and Il-6 differed, since only the effect of Il-6 on spreading was sensitive to pertussis toxin. Both Il-1 beta and Il-6 required protein synthesis to stimulate spreading, since cycloheximide inhibited the effect of the cytokines. Induction of an autocrine loop of Il-6 in the SK-BR-3 cells by Il-1 beta was unlikely, since after stimulation with Il-1 beta, no induction of Il-6 activity was measured, nor was inhibition of stimulated spreading seen in the presence of an antiserum against Il-6. Addition of Il-8 or of an antiserum against Il-8 did not affect spreading. We concluded that Il-1 and Il-6 could act as motility factors for human breast carcinoma cells, in both an independent and an additive way.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Verhasselt B,Van Damme J,van Larebeke N,Put W,Bracke M,De Potter C,Mareel Msubject
Has Abstractpub_date
1992-12-01 00:00:00pages
449-57issue
2eissn
0171-9335issn
1618-1298journal_volume
59pub_type
杂志文章abstract::In solid body tissues, environmental biomechanics is indispensable for tissue homeostasis. While characteristics of homeostasis include morphogenesis, proliferation and differentiation, the influences through biomechanics in corneal keratinocytes are poorly understood. Here we show for the first time that corneal kera...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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