Abstract：
:Extraovarian estrogen production is localized mainly in stromal vascular cells from adipose tissue, which contain the aromatase activity necessary for the conversion of androgens to estrogens. Induction of aromatase activity in these cells by glucocorticoids occurs only in the presence of a serum factor, the properties and origin of which are unknown (serum-derived aromatase stimulating activity, serum-ASA). Cells of the breast cancer line MDA-MB231 produce a factor resembling serum-ASA, as it is able to completely replace serum during the induction of aromatase by glucocorticoids. MDA-MB231-derived ASA is destroyed by proteolytic digestion and is purified about 100-fold by ultrafiltration of MDA-MB231 conditioned media and anion exchange chromatography.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Schmidt M,Löffler Gsubject
Has Abstractpub_date
1994-02-01 00:00:00pages
96-101issue
1eissn
0171-9335issn
1618-1298journal_volume
63pub_type
杂志文章abstract：:Polycationic derivatives of ferritin containing primary amino groups (CFah) or tertiary amino groups (CFdmp) were potent platelet agonists inducing shape change, aggregation and secretion, but also agglutination in the presence of EDTA. Pretreatment of platelets with neuraminidase, PGE1, indomethacin, or creatine kina...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:Neurofilament (NF) structure and ability to form cross-bridges were examined by quick-freeze deep-etch mica and low-angle rotary-shadow electron microscopy in NFs purified from bovine spinal cord and reassembled in various combinations of NF subunits. When NFs were reassembled from triplet proteins, NF-L, NF-M and NF-...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:Next to the already available mouse monoclonal and laboratory animal-derived polyclonal antibodies, recombinant antibodies offer an additional and virtually unlimited arsenal of new immunohistochemical research tools. The major advantages of recombinant antibodies are their rapid and easy generation against virtually ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2004.12.025
更新日期：2005-04-01 00:00:00
abstract：:Appreciable amounts of glycosaminoglycans have been found by immunocytochemistry within mature elastin fibers of human dermis. On thin sections, elastin fibers showed antigenic sites for monoclonal antibodies recognizing the unsaturated units remaining after digestion of hyaluronic acid with Streptomyces hyaluronidase...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:An increase in light intensity induces a depolarization in retinal ON-bipolar cells via a reduced glutamate release from presynaptic photoreceptor cells. The underlying transduction cascade in the dendritic tips of ON-bipolar cells involves mGluR6 glutamate receptors signaling to TRPM1 proteins that are an indispensab...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2015.06.005
更新日期：2015-07-01 00:00:00
abstract：:This study reports the ultrastructural detection of fucosyl residues at the surface of axenically grown roots of Zea mays. The method used involved sequential binding of UeA lectin to the root and coupling of the bound UeA with fucosyl ferritin. Superficial dense ferritin labelling was found in the slime droplet enshe...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:To study molecules secreted from cultured plant cells that promote development, maize microspores were transferred into culture and the conditioned media were collected over time and analysed. Electrophoresis indicated that both non-glycosylated and glycosylated proteins including arabinogalactan proteins (AGPs) appea...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00378
更新日期：2004-07-01 00:00:00
abstract：:Dipyridamole (DPD), a widely used anti-thrombotic agent, inhibits in vitro directional migration of 10T1/2 mouse embryo cells in a dose dependent way. The inhibition is detectable at DPD concentrations comparable to those in human plasma after oral intake of the drug. Using a model for quantitative shape change analys...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:During myocardial infarction, it is well known that the cytoplasm of cardiac myocytes becomes more acidic due to lactate accumulation. The resulting necrosis is believed to result, at least in part, from the leakage of lysosomal enzymes into the cytoplasm. In addition, it has previously been shown that cytoplasm acidi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:The fine structure of a joint receptor (R10) in a spider leg (Zygiella x-notata) was examined with light and electron microscopy. The R10 receptor consists of a compact ganglion which is situated near the dorsal joint membrane of the femur/patella joint. Each of the ten sensory cells comprising the ganglion sends one ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:In recent years, several antagonists of alpha(v)beta3 have been used to develop therapeutic approaches to the treatment of melanoma neoplasia. We studied the effects of anti-alpha(v)-integrin-blocking antibodies on attached M21 melanoma cells, the cellular distribution of alpha(v)-integrin and the molecular organizati...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00067
更新日期：2000-07-01 00:00:00
abstract：:We have investigated the effects of bafilomycin A1, a specific inhibitor of vacuolar type H(+)-ATPase, on the secretion and intracellular distribution of prolactin in cultured rat pituitary tumor cells (GH3 cells). Pulse-labeling the cells with L-[35S]methionine for 5 min and subsequently incubating in chase medium co...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:Procaine-treated eggs can be penetrated by more than one spermatozoon. Supernumerary male pronuclei can fuse with the female one giving raise to multipolar spindles or remain isolated within the egg's cytoplasm forming their own spindle. In all types of multiple mitotic figures (asters and spindles) the distribution o...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:A method using proteins covalently coupled to glass surfaces has been applied to studies on the differentiation of neuroblasts. Neuroblastoma cells from clones N 18 and NIE 115 adhere to surfaces coated with fibronectin or laminin and extend rapidly growth cone-containing neurites. Some lectin-coated surfaces are also...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:The microtubule-associated protein 2 (MAP2) and its juvenile splicing variant MAP2c contain a phosphorylation site at Ser136 which is part of a Ser-Pro motif. This site lies within the N-terminal region common to MAP2b and MAP2c. It has been mapped by site-directed mutagenesis of recombinant MAP2c and by a monoclonal ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:In the present study, a systematic examination of the relative expression pattern of the nuclear retinoid receptors (RAR and RXR) in various liver cells was performed. Our data demonstrate that RXRalpha is the dominant receptor in all liver cells, and that RARbeta is also expressed at a high level in most cells. More ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80078-2
更新日期：1998-10-01 00:00:00
abstract：:While many studies of erythropoiesis have focused on interactions between developing cells and the extracellular matrix, scant attention has been paid to the role of erythroid-macrophage interactions in this process. Murine erythroleukemia (MEL) cell lines have been used extensively in these studies to define erythroi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:We have examined the importance of cell surface heparan sulfate proteoglycans (HSPG) in fibroblast growth factor (FGF) signaling. 3T3 cells grown under conventional conditions were much more sensitive to FGF-2 compared to FGF-1. However, cells were equally sensitive to FGF-1 and FGF-2 using conditions which reduced th...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:Embryonic chick myoblasts possess an extensive network of cytoplasmic microtubules which emanate from a single, perinuclear centrosome containing a microtubule-organizing center (MTOC) and the centrioles. However, after myoblasts fuse into myotubes the centrosome is no longer apparent, and instead long parallel arrays...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:This paper explores the mode of action of the tranquillizers chloral hydrate and diazepam during fertilization and mitosis of the first reproductive cell cycles in sea urchin eggs. Most striking effects of these drugs are the alteration of centrosomal material and the abnormal microtubule configurations during exposur...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80041-1
更新日期：1998-01-01 00:00:00
abstract：:Intact follicles as well as defolliculated oocytes of the mouse were studied by freeze-fracture electron microscopy. In intact follicles the oocyte plasma membrane shows two prominent types of intra-membrane particle array:gap junctions and yet undescribed rhombic particle arrays. The gap junctions vary in size (from ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:In this study we have used primary cultures of porcine superior cervical ganglia as a model system to study exo-endocytosis in sympathetic neurons. Pure neuronal cultures with a defined noradrenergic phenotype can be obtained when antimitotics are included in the culture medium, and the high yield from prenatal piglet...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Morphological explanations for the effects of cytochalasin B (CB) on the digestive vacuole system of Paramecium caudatum were sought using electron-microscopic studies. Cytochalasin B (0.3 mM) essentially stopped vacuole release without stopping vacuole growth. Acidosome fusion with the new vacuoles was inhibited and ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:The recently established in vitro assay of condensation-sorting of pancreatic enzymes to the zymogen granule membrane (ZGM) (Dartsch, H., R. Kleene, H. F. Kern: In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells. Eur. J. Cell Biol. 75, 211-222 (1998)) was used to study the invol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80009-0
更新日期：1999-02-01 00:00:00
abstract：:In the present study the role of endothelin (ET) and its receptors (ETA-R and ETB-R) in cellular mechanisms underlying the resistance of astroglial cells to low oxygen level and development of hypoxia has been investigated. To define the influences of ET and its receptors on survival and on antigenic as well as morpho...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2004.12.030
更新日期：2005-06-01 00:00:00
abstract：:Vinculin, discovered in 1979 (Geiger, 1979), is an adapter protein with binding sites for more than 15 proteins. Biochemical and structural analyses have contributed to detailed knowledge about potential binding partners and the understanding of how their binding may be regulated. Despite all this information the mole...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2010.06.007
更新日期：2011-02-01 00:00:00
abstract：:When resting human blood platelets are stimulated with thrombin, 50 to 60% of the G-actin polymerizes to F-actin within 60 seconds. The increase in F-actin is correlated with a corresponding decrease in the complex of G-actin with T beta 4. Within 5 seconds after stimulation, nucleation sites for pyrene actin polymeri...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:Despite its frequent inactivation in human breast cancers, the role of p21(Cip1) (p21) in morphological plasticity of normal mammary epithelial cells is still poorly understood. To address this question, we have investigated the consequences of p21 silencing in two-dimensional (2D) morphogenesis of untransformed human...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2011.03.002
更新日期：2011-08-01 00:00:00
abstract：:The AtT20 pituitary cell is the one that was originally used to define the pathways taken by secretory proteins in mammalian cells. It possesses two secretory pathways, the constitutive for immediate secretion and the regulated for accumulation and release under hormonal stimulation. It is in the regulated pathway, mo...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00365
更新日期：2004-04-01 00:00:00
abstract：:The serine/arginine rich proteins (SR proteins) are members of a family of RNA binding proteins involved in regulating various features of RNA metabolism, including pre-mRNA constitutive and alternative splicing. In humans, a total of 12 SR splicing factors (SRSFs) namely SRSF1-SRSF12 have been reported. SRSF3, the sm...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2020.151099
更新日期：2020-08-01 00:00:00
