Abstract:
:The arrangement of carbohydrate molecules on surfaces of fungal cells may play an important role in nonself recognition of these microorganisms by potential invertebrate hosts. Changes in the ability of various galactose and mannose-specific lectins to bind to surface components on cell walls of the insect pathogen Paecilomyces farinosus were therefore examined during growth and differentiation of the fungus. Fluorescein isothiocyanate conjugates of concanavalin A (Con A, specific for alpha-D-mannose) and peanut agglutinin (PNA, beta-D-galactose) bound inconsistently to blastospores and weakly to mycelia except at apical regions where strong fluorescence was observed. Labeling patterns were similar on cells tested with a galactose-specific lectin purified from Spodoptera exigua (beet armyworm) hemolymph, but Bandeiraea simplicifolia lectin (BS-I alpha-D-galactose) bound only to mycelia. Electron microscopy using ferritin and gold probes showed that the galactomannans are located in a loosely bound coating on the cell wall surface. Variations in lectin binding patterns are apparently due to absence (e.g., by shedding) of the coat or to rearrangement of carbohydrate components in the coat. Staining of Western blots of dithiothreitol (DTT) cell wall extracts further indicated that the BS-I-binding entity is a unique component of the mycelial surface since, as in the fluorescence studies, blastospore preparations were not labeled. Staining of blastospore blots with other galactose-specific probes (e.g., PNA) was comparable to staining of mycelial blots.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Pendland JC,Boucias DGsubject
Has Abstractpub_date
1993-04-01 00:00:00pages
322-30issue
2eissn
0171-9335issn
1618-1298journal_volume
60pub_type
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journal_title:European journal of cell biology
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更新日期:1986-03-01 00:00:00
abstract::Kynurenic acid (KYNA), a tryptophan metabolite in the kynurenine pathway, is protective against various insults. However, the molecular mechanism of this protective effect has not been identified. In this study, we examined the protective effects of KYNA against 1-methyl-4-phenylpyridinium (MPP(+)), the best-character...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1997-08-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1981-02-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1997-04-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1990-02-01 00:00:00
abstract::Human ECV304 cells respond reproducibly by tube formation to complex basement membrane matrices. Laminins are major glycoproteins of basement membranes. We therefore studied the ability of ECV304 cells to attach to defined laminin isoforms and to fibronectin, and identified the involved laminin receptors. The cells bo...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1993-02-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1995-09-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1988-08-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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