Abstract:
:Native tetrameric alpha 2-macroglobulin molecules (alpha 2M) can be converted into a population of dimers by incubation with various divalent cations such as Zn, Cd, Mg, Cu, Ni, Co. This dissociation is completed within 30 min at 37 degrees C. These dimers have a characteristic shape and a size of about 16 X 8 nm, and appear to be the half of the native alpha 2M molecule which has a clear tetrameric structure as seen in the electron microscope. At room temperature or below, dimers obtained with 5 to 100 mM Zn++ can reassociate in long linear polymers which display a regular chain-like arrangement and a helical periodicity. The structural characteristics of this polymer are described. The trypsin inhibitory capacity of Zn++-treated alpha 2M has been studied in an attempt to correlate its Zn++-induced conformational changes with its functional modifications.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Couture-Tosi E,Tapon-Bretaudière J,Pochon F,Barray M,Bros A,Delain Esubject
Has Abstractpub_date
1986-12-01 00:00:00pages
359-64issue
2eissn
0171-9335issn
1618-1298journal_volume
42pub_type
杂志文章abstract::Cytolocalization of stomatin, an integral membrane protein also called erythrocyte band 7.2b, was investigated in a human epithelial cell line in which the expression of this protein is up-regulated after treatment with interleukin-6 and dexamethasone. A monoclonal antibody against stomatin was used to perform immunof...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1979-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1986-12-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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更新日期:1996-03-01 00:00:00
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