Pros and cons of fish skin cells in culture: long-term full skin and short-term scale cell culture from rainbow trout, Oncorhynchus mykiss.

Abstract:

:Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new fish skin cell culture. We further demonstrated that epithelial cells derived from the scales survived in the artificial micro-environment of surrounding fibroblast-like cells. Also, antibody staining indicated that both cell types proliferated and started to build connections with the other cell type. It seems that it is possible to generate an 'artificial skin' with two different cell types. This could lead to the development of a three-dimensional test system, which might be a better in vitro representative of fish skin in vivo than individual skin cell lines.

journal_name

Eur J Cell Biol

authors

Rakers S,Klinger M,Kruse C,Gebert M

doi

10.1016/j.ejcb.2011.08.003

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

1041-51

issue

12

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(11)00168-3

journal_volume

90

pub_type

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