Abstract:
:Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new fish skin cell culture. We further demonstrated that epithelial cells derived from the scales survived in the artificial micro-environment of surrounding fibroblast-like cells. Also, antibody staining indicated that both cell types proliferated and started to build connections with the other cell type. It seems that it is possible to generate an 'artificial skin' with two different cell types. This could lead to the development of a three-dimensional test system, which might be a better in vitro representative of fish skin in vivo than individual skin cell lines.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Rakers S,Klinger M,Kruse C,Gebert Mdoi
10.1016/j.ejcb.2011.08.003subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1041-51issue
12eissn
0171-9335issn
1618-1298pii
S0171-9335(11)00168-3journal_volume
90pub_type
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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doi:10.1016/j.ejcb.2005.04.004
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::In recent years, several antagonists of alpha(v)beta3 have been used to develop therapeutic approaches to the treatment of melanoma neoplasia. We studied the effects of anti-alpha(v)-integrin-blocking antibodies on attached M21 melanoma cells, the cellular distribution of alpha(v)-integrin and the molecular organizati...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2015.05.005
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
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doi:
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doi:
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doi:
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
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doi:
更新日期:1982-11-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
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