Structural and immunocytochemical alterations in eye lens fiber cells from Cx46 and Cx50 knockout mice.

Abstract:

:In the current study we describe the changes of overall organization of lens fiber cells in connexin 46 (Cx46) and connexin 50 (Cx50) knockout mice. Morphometric analyses and the application of immunocytochemical techniques revealed that in Cx46 knockout lens (Cx46 -/-), where Cx50 is expressed alone, the postnatal differentiation of secondary fiber cells proceeds faster and is characterized by an increased number of smaller fiber cells. Conversely, in Cx50 knockout mice (Cx50 -/-), the lenticular mass is considerably reduced and characterized by a small number of fiber cells added during the postnatal period. The process of terminal differentiation was impaired and generated larger fiber cells still possessing cytoplasmic organelles. Freeze-fracture and fracture labeling revealed that the junctional assembly, packing organization and topographic interactions between connexons and MP26 differed when Cx46 and Cx50 were co-assembled in the wild-type or expressed separately in the two distinct knockout phenotypes. Filipin cytochemistry provided indirect evidence that Cx46 and Cx50 expressed alone are recruited into different lipid environments. Our results represent the structural proof that interaction of connexins and MP26 contributes to the overall organization of the fiber cells.

journal_name

Eur J Cell Biol

authors

Dunia I,Cibert C,Gong X,Xia CH,Recouvreur M,Levy E,Kumar N,Bloemendal H,Benedetti EL

doi

10.1016/j.ejcb.2006.03.006

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

729-52

issue

8

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(06)00067-7

journal_volume

85

pub_type

杂志文章
  • Identification of a transformation-sensitive 110-kDa plasma membrane glycoprotein of rat hepatocytes.

    abstract::Monoclonal antibodies were used to define cell surface antigens which are present on rat hepatocytes but are absent from hepatoma cells. One monoclonal antibody, referred to as Be 9.2, recognizes a major component of purified rat liver plasma membranes with a Mr of 110 000. This antigen (gp110) was not found in the tr...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Becker A,Neumeier R,Park CS,Gossrau R,Reutter W

    更新日期:1986-01-01 00:00:00

  • Centrioles are lost as embryonic myoblasts fuse into myotubes in vitro.

    abstract::Embryonic chick myoblasts possess an extensive network of cytoplasmic microtubules which emanate from a single, perinuclear centrosome containing a microtubule-organizing center (MTOC) and the centrioles. However, after myoblasts fuse into myotubes the centrosome is no longer apparent, and instead long parallel arrays...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Connolly JA,Kiosses BW,Kalnins VI

    更新日期:1986-01-01 00:00:00

  • Localization of Gts1p in cortical actin patches of yeast and its possible role in endocytosis.

    abstract::Herein we report that Gts1p fused with green-fluorescent protein (GFP) is localized in the cortical actin patch besides nuclei in yeast and the cortical Gts1p changed its position together with the patch depending on the cell-cycle phase, while nuclear Gts1p accumulated predominantly in the budding phase. Whereas Gts1...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2007.02.005

    authors: Yaguchi S,Shen H,Tsurugi K

    更新日期:2007-05-01 00:00:00

  • Expression of vimentin and nuclear lamins during the in vitro differentiation of human promyelocytic leukemia cells HL-60.

    abstract::We have reported previously that the human promyelocytic leukemia cell line HL-60, in its undifferentiated state, is devoid of cytoplasmic intermediate filament proteins and nuclear lamins A and C, but does express lamin B. Using immunofluorescence and immunoblotting techniques, we have further investigated the expres...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Paulin-Levasseur M,Giese G,Scherbarth A,Traub P

    更新日期:1989-12-01 00:00:00

  • RIP3 and AtKinesin-13A - a novel interaction linking Rho proteins of plants to microtubules.

    abstract::RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 ha...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2010.08.003

    authors: Mucha E,Hoefle C,Hückelhoven R,Berken A

    更新日期:2010-12-01 00:00:00

  • Modulation of the digestive lysosomal system in Paramecium caudatum. III. Morphological effects of cytochalasin B.

    abstract::Morphological explanations for the effects of cytochalasin B (CB) on the digestive vacuole system of Paramecium caudatum were sought using electron-microscopic studies. Cytochalasin B (0.3 mM) essentially stopped vacuole release without stopping vacuole growth. Acidosome fusion with the new vacuoles was inhibited and ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Allen RD,Fok AK

    更新日期:1985-05-01 00:00:00

  • Coordinated regulation of procollagens I and III and their post-translational enzymes by dissipation of mechanical tension in human dermal fibroblasts.

    abstract::Mechanical tension governs fibroblast proliferation and survival and the homeostasis of the extracellular matrix to adapt its resistance to the mechanical requirements of the organs. To consolidate this view, we analysed the effect of tension release on the expression of molecules involved in the architecture and stab...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00181

    authors: Lambert CA,Colige AC,Lapière CM,Nusgens BV

    更新日期:2001-07-01 00:00:00

  • Multimeric complexes of differentiation-inducing protein bound to DNA.

    abstract::Myeloid hematopoietic precursor cells are induced to differentiate by the macrophage and granulocyte differentiation-inducing protein MGI-2 (DF). This differentiation-inducing protein bound to double-stranded but not to single-stranded mammalian DNA. The bound MGI-2 was not eluted by high salt, but was eluted by sodiu...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Weisinger G,Korn AP,Sachs L

    更新日期:1985-05-01 00:00:00

  • The secretory lectin ZG16p mediates sorting of enzyme proteins to the zymogen granule membrane in pancreatic acinar cells.

    abstract::The recently established in vitro assay of condensation-sorting of pancreatic enzymes to the zymogen granule membrane (ZGM) (Dartsch, H., R. Kleene, H. F. Kern: In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells. Eur. J. Cell Biol. 75, 211-222 (1998)) was used to study the invol...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/S0171-9335(99)80009-0

    authors: Kleene R,Dartsch H,Kern HF

    更新日期:1999-02-01 00:00:00

  • Colocalization of stomatin (band 7.2b) and actin microfilaments in UAC epithelial cells.

    abstract::Cytolocalization of stomatin, an integral membrane protein also called erythrocyte band 7.2b, was investigated in a human epithelial cell line in which the expression of this protein is up-regulated after treatment with interleukin-6 and dexamethasone. A monoclonal antibody against stomatin was used to perform immunof...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Snyers L,Thinès-Sempoux D,Prohaska R

    更新日期:1997-07-01 00:00:00

  • The entry of sporozoites of Theileria parva into bovine lymphocytes in vitro. Electron microscopic observations.

    abstract::The entry of sporozoites of Theileria parva into bovine lymphoid cells in vitro was studied with the electron microscope. Endocytosis is completed in less than 10 min. No local mobilization of actin or other cytoskeletal elements is detected in the cytoplasm of the cell being invaded and no engulfing pseudopods are fo...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Fawcett DW,Doxsey S,Stagg DA,Young AS

    更新日期:1982-04-01 00:00:00

  • Immunocytochemical electron microscopic study and Western blot analysis of caldesmon and calponin in striated muscle of the fruit fly Drosophila melanogaster and in several muscle cell types of the earthworm Eisenia foetida.

    abstract::Caldesmon and calponin are two proteins that are characteristic of vertebrate smooth muscle. In invertebrates, caldesmon has only been studied in some molluscan muscles, and no previous references to calponin have been found. The aim of this paper was to investigate the presence and distribution of caldesmon and calpo...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Royuela M,Fraile B,Picazo ML,Paniagua R

    更新日期:1997-01-01 00:00:00

  • Effects of surface-active alkyltrimethylammonium salts on concanavalin A-mediated agglutination in Acanthamoeba castellanii.

    abstract::Pretreatment of Acanthamoeba castellanii (Neff strain) with sublytic concentrations of surface-active alkyltrimethylammonium salts (C12, C14, C16) enhanced ConA-mediated agglutination of the amoebae. Treatment with the surfactants alone did not affect the "spontaneous" agglutination of the amoebae. Electron microscopi...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Paatero G,Isomaa B,Johansson M,Kiiskinen G

    更新日期:1981-08-01 00:00:00

  • Differential sorting of SNAP-25a and SNAP-25b proteins in neuroblastoma cells.

    abstract::Exocytosis is mediated by high-affinity interactions between different SNARE proteins. The existence of several variants of each SNARE protein suggests that the specificity of fusion may be directed by unique combination of SNARE family members. We examined if two alternatively spliced variants of synaptosomal-associa...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00106

    authors: Andersson J,Fried G,Lilja L,Meister B,Bark C

    更新日期:2000-11-01 00:00:00

  • Gap junctions and rhombic particle arrays in the freeze-fractured mouse oocyte-follicle cell complex.

    abstract::Intact follicles as well as defolliculated oocytes of the mouse were studied by freeze-fracture electron microscopy. In intact follicles the oocyte plasma membrane shows two prominent types of intra-membrane particle array:gap junctions and yet undescribed rhombic particle arrays. The gap junctions vary in size (from ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: van den Hoef MH,Bluemink JG,de Laat SW

    更新日期:1984-11-01 00:00:00

  • The area composita of adhering junctions connecting heart muscle cells of vertebrates. I. Molecular definition in intercalated disks of cardiomyocytes by immunoelectron microscopy of desmosomal proteins.

    abstract::Among sarcomeric muscles the cardiac muscle cells are unique by, inter alia, a systemic and extended cell-cell contact structure, the intercalated disk (ID), comprising frequent and closely spaced arrays of plaque-coated cell-cell adhering junctions (AJs). As some of these junctions may look somewhat like desmosomes a...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2005.11.003

    authors: Franke WW,Borrmann CM,Grund C,Pieperhoff S

    更新日期:2006-02-01 00:00:00

  • Evidence for a homolog of the yeast cell cycle regulatory gene product of cdc2+ in Physarum polycephalum.

    abstract::Evidence for the presence of a Cdc2-like protein in Physarum polycephalum has been obtained using a peptide antibody directed against a highly conserved amino acid sequence near the N-terminal end of Cdc2, Cdc28 and Cdc2HS. The antibody detected a 34 kDa cytoplasmic protein, similar in apparent size to Cdc2 in yeast a...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Shipley GL,Sauer HW

    更新日期:1989-02-01 00:00:00

  • Cell adhesion-dependent regulation of cell growth during sea urchin development.

    abstract::In the sea urchin embryo there are at least two cell adhesion molecules related to mammalian cadherins, one of them, similar to E-cadherin, is expressed in embryos at very early developmental stages, the second appears at the blastula stage (G. Ghersi et al. Mech. Dev. 41, 47-55 (1993)). We show here that when sea urc...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Ghersi G,Salamone M,Levi G,Vittorelli ML

    更新日期:1996-03-01 00:00:00

  • The effect of dexamethasone on transferrin secretion by cultured fetal rat hepatocytes.

    abstract::Cultured fetal rat hepatocytes derived from 12, 15 and 19-day gestation rats are capable of secreting transferrin. When dexamethasone is added to the medium an increased secretion rate is observed. The changes in secretion rates in control as well as dexamethasone-treated cells during culture have been shown to correl...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Kraemer M,Vassy J,Brighton V,Fuller S,Yeoh G

    更新日期:1986-10-01 00:00:00

  • A simplified protocol for the isolation and culture of cardiomyocytes and progenitor cells from neonatal mouse ventricles.

    abstract::The neonatal heart is a very useful tool for the study of biochemical pathways and properties of cardiomyocytes and as it has the potential to regenerate for a brief period of time from birth; it is also useful to study cardiac regeneration. However, as the heart matures, this proficiency for regeneration is reduced. ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2015.06.009

    authors: Vidyasekar P,Shyamsunder P,Santhakumar R,Arun R,Verma RS

    更新日期:2015-10-01 00:00:00

  • Pros and cons of fish skin cells in culture: long-term full skin and short-term scale cell culture from rainbow trout, Oncorhynchus mykiss.

    abstract::Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2011.08.003

    authors: Rakers S,Klinger M,Kruse C,Gebert M

    更新日期:2011-12-01 00:00:00

  • Yeast dynamin Vps1 associates with clathrin to facilitate vesicular trafficking and controls Golgi homeostasis.

    abstract::The yeast dynamin Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains u...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2017.02.004

    authors: Goud Gadila SK,Williams M,Saimani U,Delgado Cruz M,Makaraci P,Woodman S,Short JC,McDermott H,Kim K

    更新日期:2017-03-01 00:00:00

  • Lamins A and C are differentially dysfunctional in autosomal dominant Emery-Dreifuss muscular dystrophy.

    abstract::Mutations in the LMNA gene, which encodes nuclear lamins A and C by alternative splicing, can give rise to Emery-Dreifuss muscular dystrophy. The mechanism by which lamins A and C separately contribute to this molecular phenotype is unknown. To address this question we examined ten LMNA mutations exogenously expressed...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2005.04.004

    authors: Motsch I,Kaluarachchi M,Emerson LJ,Brown CA,Brown SC,Dabauvalle MC,Ellis JA

    更新日期:2005-09-01 00:00:00

  • Hyaluronan fragments: an information-rich system.

    abstract::Hyaluronan is a straight chain, glycosaminoglycan polymer of the extracellular matrix composed of repeating units of the disaccharide [-D-glucuronic acid-beta1,3-N-acetyl-D-glucosamine-beta1,4-]n. Hyaluronan is synthesized in mammals by at least three synthases with products of varying chain lengths. It has an extraor...

    journal_title:European journal of cell biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ejcb.2006.05.009

    authors: Stern R,Asari AA,Sugahara KN

    更新日期:2006-08-01 00:00:00

  • Orthobunyaviruses and innate immunity induction: alieNSs vs. PredatoRRs.

    abstract::The taxonomic group of Orthobunyaviruses is gaining increased attention, as several emerging members are causing devastating illnesses among humans and livestock. These viruses are transmitted to mammals by arthropods (mostly mosquitoes) during the blood meal. The nature of their genomic RNA predisposes orthobunyaviru...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2015.06.001

    authors: Schoen A,Weber F

    更新日期:2015-07-01 00:00:00

  • A caudal-type homeobox gene activity associated with the development and regeneration of the liver.

    abstract::Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Doll U,Niessing J

    更新日期:1996-07-01 00:00:00

  • Intrinsic fluctuations of phosphoinositol levels are involved in the progression of the naturally synchronous cell cycle in Physarum polycephalum.

    abstract::Using [3H]myo-inositol incorporation, changes in phosphoinositol (PI) metabolism at different cell cycle stages in the myxomycete Physarum polycephalum were examined by column chromatography. Two base levels for the inositol trisphosphate (IP3) fraction were determined: a low one for S-phase and a higher one for G2 ph...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Belyavskyi M,Sauer HW

    更新日期:1992-08-01 00:00:00

  • Rho GTPase signaling in Dictyostelium discoideum: insights from the genome.

    abstract::Rho GTPases are ubiquitously expressed across the eukaryotes where they act as molecular switches participating in the regulation of many cellular processes. We present an inventory of proteins involved in Rho-regulated signaling pathways in Dictyostelium discoideum that have been identified in the completed genome se...

    journal_title:European journal of cell biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ejcb.2006.04.011

    authors: Vlahou G,Rivero F

    更新日期:2006-09-01 00:00:00

  • p21(Cip1) regulates cell-substrate adhesion and interphase microtubule dynamics in untransformed human mammary epithelial cells.

    abstract::Despite its frequent inactivation in human breast cancers, the role of p21(Cip1) (p21) in morphological plasticity of normal mammary epithelial cells is still poorly understood. To address this question, we have investigated the consequences of p21 silencing in two-dimensional (2D) morphogenesis of untransformed human...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2011.03.002

    authors: Bouchet BP,Fauvet F,Grelier G,Galmarini CM,Puisieux A

    更新日期:2011-08-01 00:00:00

  • Effects of Clostridium botulinum C2 toxin and cytochalasin D on in vitro invasiveness, motility and F-actin content of a murine T-lymphoma cell line.

    abstract::In order to investigate the role of microfilaments in the crawling movements of lymphoid cells, we have analyzed the effects of botulinum C2 toxin and of cytochalasin D (cytoD) on the actin cytoskeleton and on the motility of a BW5147 T-lymphoma-derived cell line. Actin was ADP-ribosylated by C2 toxin in the living ce...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Verschueren H,van der Taelen I,Dewit J,De Braekeleer J,De Baetselier P,Aktories K,Just I

    更新日期:1995-04-01 00:00:00