Abstract:
:In the current study we describe the changes of overall organization of lens fiber cells in connexin 46 (Cx46) and connexin 50 (Cx50) knockout mice. Morphometric analyses and the application of immunocytochemical techniques revealed that in Cx46 knockout lens (Cx46 -/-), where Cx50 is expressed alone, the postnatal differentiation of secondary fiber cells proceeds faster and is characterized by an increased number of smaller fiber cells. Conversely, in Cx50 knockout mice (Cx50 -/-), the lenticular mass is considerably reduced and characterized by a small number of fiber cells added during the postnatal period. The process of terminal differentiation was impaired and generated larger fiber cells still possessing cytoplasmic organelles. Freeze-fracture and fracture labeling revealed that the junctional assembly, packing organization and topographic interactions between connexons and MP26 differed when Cx46 and Cx50 were co-assembled in the wild-type or expressed separately in the two distinct knockout phenotypes. Filipin cytochemistry provided indirect evidence that Cx46 and Cx50 expressed alone are recruited into different lipid environments. Our results represent the structural proof that interaction of connexins and MP26 contributes to the overall organization of the fiber cells.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Dunia I,Cibert C,Gong X,Xia CH,Recouvreur M,Levy E,Kumar N,Bloemendal H,Benedetti ELdoi
10.1016/j.ejcb.2006.03.006subject
Has Abstractpub_date
2006-08-01 00:00:00pages
729-52issue
8eissn
0171-9335issn
1618-1298pii
S0171-9335(06)00067-7journal_volume
85pub_type
杂志文章abstract::Monoclonal antibodies were used to define cell surface antigens which are present on rat hepatocytes but are absent from hepatoma cells. One monoclonal antibody, referred to as Be 9.2, recognizes a major component of purified rat liver plasma membranes with a Mr of 110 000. This antigen (gp110) was not found in the tr...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Embryonic chick myoblasts possess an extensive network of cytoplasmic microtubules which emanate from a single, perinuclear centrosome containing a microtubule-organizing center (MTOC) and the centrioles. However, after myoblasts fuse into myotubes the centrosome is no longer apparent, and instead long parallel arrays...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Herein we report that Gts1p fused with green-fluorescent protein (GFP) is localized in the cortical actin patch besides nuclei in yeast and the cortical Gts1p changed its position together with the patch depending on the cell-cycle phase, while nuclear Gts1p accumulated predominantly in the budding phase. Whereas Gts1...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2007.02.005
更新日期:2007-05-01 00:00:00
abstract::We have reported previously that the human promyelocytic leukemia cell line HL-60, in its undifferentiated state, is devoid of cytoplasmic intermediate filament proteins and nuclear lamins A and C, but does express lamin B. Using immunofluorescence and immunoblotting techniques, we have further investigated the expres...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 ha...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2010.08.003
更新日期:2010-12-01 00:00:00
abstract::Morphological explanations for the effects of cytochalasin B (CB) on the digestive vacuole system of Paramecium caudatum were sought using electron-microscopic studies. Cytochalasin B (0.3 mM) essentially stopped vacuole release without stopping vacuole growth. Acidosome fusion with the new vacuoles was inhibited and ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::Mechanical tension governs fibroblast proliferation and survival and the homeostasis of the extracellular matrix to adapt its resistance to the mechanical requirements of the organs. To consolidate this view, we analysed the effect of tension release on the expression of molecules involved in the architecture and stab...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00181
更新日期:2001-07-01 00:00:00
abstract::Myeloid hematopoietic precursor cells are induced to differentiate by the macrophage and granulocyte differentiation-inducing protein MGI-2 (DF). This differentiation-inducing protein bound to double-stranded but not to single-stranded mammalian DNA. The bound MGI-2 was not eluted by high salt, but was eluted by sodiu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::The recently established in vitro assay of condensation-sorting of pancreatic enzymes to the zymogen granule membrane (ZGM) (Dartsch, H., R. Kleene, H. F. Kern: In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells. Eur. J. Cell Biol. 75, 211-222 (1998)) was used to study the invol...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(99)80009-0
更新日期:1999-02-01 00:00:00
abstract::Cytolocalization of stomatin, an integral membrane protein also called erythrocyte band 7.2b, was investigated in a human epithelial cell line in which the expression of this protein is up-regulated after treatment with interleukin-6 and dexamethasone. A monoclonal antibody against stomatin was used to perform immunof...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::The entry of sporozoites of Theileria parva into bovine lymphoid cells in vitro was studied with the electron microscope. Endocytosis is completed in less than 10 min. No local mobilization of actin or other cytoskeletal elements is detected in the cytoplasm of the cell being invaded and no engulfing pseudopods are fo...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-04-01 00:00:00
abstract::Caldesmon and calponin are two proteins that are characteristic of vertebrate smooth muscle. In invertebrates, caldesmon has only been studied in some molluscan muscles, and no previous references to calponin have been found. The aim of this paper was to investigate the presence and distribution of caldesmon and calpo...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Pretreatment of Acanthamoeba castellanii (Neff strain) with sublytic concentrations of surface-active alkyltrimethylammonium salts (C12, C14, C16) enhanced ConA-mediated agglutination of the amoebae. Treatment with the surfactants alone did not affect the "spontaneous" agglutination of the amoebae. Electron microscopi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::Exocytosis is mediated by high-affinity interactions between different SNARE proteins. The existence of several variants of each SNARE protein suggests that the specificity of fusion may be directed by unique combination of SNARE family members. We examined if two alternatively spliced variants of synaptosomal-associa...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00106
更新日期:2000-11-01 00:00:00
abstract::Intact follicles as well as defolliculated oocytes of the mouse were studied by freeze-fracture electron microscopy. In intact follicles the oocyte plasma membrane shows two prominent types of intra-membrane particle array:gap junctions and yet undescribed rhombic particle arrays. The gap junctions vary in size (from ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Among sarcomeric muscles the cardiac muscle cells are unique by, inter alia, a systemic and extended cell-cell contact structure, the intercalated disk (ID), comprising frequent and closely spaced arrays of plaque-coated cell-cell adhering junctions (AJs). As some of these junctions may look somewhat like desmosomes a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.11.003
更新日期:2006-02-01 00:00:00
abstract::Evidence for the presence of a Cdc2-like protein in Physarum polycephalum has been obtained using a peptide antibody directed against a highly conserved amino acid sequence near the N-terminal end of Cdc2, Cdc28 and Cdc2HS. The antibody detected a 34 kDa cytoplasmic protein, similar in apparent size to Cdc2 in yeast a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::In the sea urchin embryo there are at least two cell adhesion molecules related to mammalian cadherins, one of them, similar to E-cadherin, is expressed in embryos at very early developmental stages, the second appears at the blastula stage (G. Ghersi et al. Mech. Dev. 41, 47-55 (1993)). We show here that when sea urc...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::Cultured fetal rat hepatocytes derived from 12, 15 and 19-day gestation rats are capable of secreting transferrin. When dexamethasone is added to the medium an increased secretion rate is observed. The changes in secretion rates in control as well as dexamethasone-treated cells during culture have been shown to correl...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
abstract::The neonatal heart is a very useful tool for the study of biochemical pathways and properties of cardiomyocytes and as it has the potential to regenerate for a brief period of time from birth; it is also useful to study cardiac regeneration. However, as the heart matures, this proficiency for regeneration is reduced. ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2015.06.009
更新日期:2015-10-01 00:00:00
abstract::Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2011.08.003
更新日期:2011-12-01 00:00:00
abstract::The yeast dynamin Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains u...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.02.004
更新日期:2017-03-01 00:00:00
abstract::Mutations in the LMNA gene, which encodes nuclear lamins A and C by alternative splicing, can give rise to Emery-Dreifuss muscular dystrophy. The mechanism by which lamins A and C separately contribute to this molecular phenotype is unknown. To address this question we examined ten LMNA mutations exogenously expressed...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.04.004
更新日期:2005-09-01 00:00:00
abstract::Hyaluronan is a straight chain, glycosaminoglycan polymer of the extracellular matrix composed of repeating units of the disaccharide [-D-glucuronic acid-beta1,3-N-acetyl-D-glucosamine-beta1,4-]n. Hyaluronan is synthesized in mammals by at least three synthases with products of varying chain lengths. It has an extraor...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2006.05.009
更新日期:2006-08-01 00:00:00
abstract::The taxonomic group of Orthobunyaviruses is gaining increased attention, as several emerging members are causing devastating illnesses among humans and livestock. These viruses are transmitted to mammals by arthropods (mostly mosquitoes) during the blood meal. The nature of their genomic RNA predisposes orthobunyaviru...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2015.06.001
更新日期:2015-07-01 00:00:00
abstract::Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::Using [3H]myo-inositol incorporation, changes in phosphoinositol (PI) metabolism at different cell cycle stages in the myxomycete Physarum polycephalum were examined by column chromatography. Two base levels for the inositol trisphosphate (IP3) fraction were determined: a low one for S-phase and a higher one for G2 ph...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::Rho GTPases are ubiquitously expressed across the eukaryotes where they act as molecular switches participating in the regulation of many cellular processes. We present an inventory of proteins involved in Rho-regulated signaling pathways in Dictyostelium discoideum that have been identified in the completed genome se...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2006.04.011
更新日期:2006-09-01 00:00:00
abstract::Despite its frequent inactivation in human breast cancers, the role of p21(Cip1) (p21) in morphological plasticity of normal mammary epithelial cells is still poorly understood. To address this question, we have investigated the consequences of p21 silencing in two-dimensional (2D) morphogenesis of untransformed human...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2011.03.002
更新日期:2011-08-01 00:00:00
abstract::In order to investigate the role of microfilaments in the crawling movements of lymphoid cells, we have analyzed the effects of botulinum C2 toxin and of cytochalasin D (cytoD) on the actin cytoskeleton and on the motility of a BW5147 T-lymphoma-derived cell line. Actin was ADP-ribosylated by C2 toxin in the living ce...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00