Filaments associated with the endoplasmic reticulum in the streaming cytoplasm of Chara corallina.

Abstract:

:Perfused Chara cells capable of resuming ATP-dependent cytoplasmic streaming in low free Ca++ solutions have been examined by electron microscopy for myosin-like filaments. Filaments 44 nm in diameter and up to 3 micron in length have been found associated with the endoplasmic reticulum that along with mitochondria, microbodies and dictyosomes from the endoplasm becomes immobilised around the sub-cortical actin bundles when ATP is depleted. Such endoplasmic filaments have not been detected in association with mitochondria or microbodies and they have not been found in the stationary cortex. These filaments are extracted from the perfused cell by ATP unless motility-inhibiting levels of cytochalasin B are present. The filaments are not detectable in cells inactivated in solutions containing high (10(-4) M) Ca++ concentrations even when the Ca++ level is subsequently lowered. Consistent with their being required for motility, cytoplasmic streaming cannot be effeiciently reactivated by ATP in such filament-depleted cells. The possibility is discussed that the filaments contain myosin and that the endoplasmic reticulum with which they are associated has a major role in generating and transmitting the motive force for streaming.

journal_name

Eur J Cell Biol

authors

Williamson RE

subject

Has Abstract

pub_date

1979-12-01 00:00:00

pages

177-83

issue

2

eissn

0171-9335

issn

1618-1298

journal_volume

20

pub_type

杂志文章
  • Terrestrial vertebrates have two keratin gene clusters; striking differences in teleost fish.

    abstract::Keratins I and II form the largest subgroups of mammalian intermediate filament (IF) proteins and account as obligatory heteropolymers for the keratin filaments of epithelia. All human type I genes except for the K18 gene are clustered on chromosome 17q21, while all type II genes form a cluster on chromosome 12q13, th...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2005.01.007

    authors: Zimek A,Weber K

    更新日期:2005-06-01 00:00:00

  • Effects of DMSO, pH, stretch and calcium on the thick filaments in an amphibian smooth muscle.

    abstract::Conventional fixation with glutaraldehyde fixatives at pH 7.4 did not preserve/promote thick filaments in Bufo smooth muscle. However, if pH was lowered to 6.0, thick filaments were present and addition of dimethyl sulfoxide (DMSO) led to a greater number of thick filaments. Stretch alone had little effect on the pres...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Bagby RM,Fisher BA

    更新日期:1979-06-01 00:00:00

  • Microtubule and centrosome distribution during sheep fertilization.

    abstract::The distribution of microtubules and centrosomes was studied during sheep fertilization by electron and immunofluorescence microscopy. Tubulin and centrosomal material was identified with monoclonal anti-alpha-tubulin and MPM-2 antibodies, respectively. In ovulated eggs, microtubules were exclusively found in the meio...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Le Guen P,Crozet N

    更新日期:1989-04-01 00:00:00

  • The multi-step process of human skin carcinogenesis: a role for p53, cyclin D1, hTERT, p16, and TSP-1.

    abstract::As proposed by Hanahan and Weinberg (2000. Cell 100, 57-70) carcinogenesis requires crucial events such as (i) genomic instability, (ii) cell cycle deregulation, (iii) induction of a telomere length maintenance mechanism, and (iv) an angiogenic switch. By comparing the expression of p53, cyclin D1, p16, hTERT, and TSP...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2006.11.002

    authors: Burnworth B,Arendt S,Muffler S,Steinkraus V,Bröcker EB,Birek C,Hartschuh W,Jauch A,Boukamp P

    更新日期:2007-12-01 00:00:00

  • RIP3 and AtKinesin-13A - a novel interaction linking Rho proteins of plants to microtubules.

    abstract::RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 ha...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2010.08.003

    authors: Mucha E,Hoefle C,Hückelhoven R,Berken A

    更新日期:2010-12-01 00:00:00

  • Coexistence of nucleosomal and various non-nucleosomal chromatin configurations in cells infected with herpes simplex virus.

    abstract::Coexistence of four different forms of chromatin was observed by electron microscopy in nuclear spread preparations of monkey kidney cells during late stages of infection with herpes simplex virus (HSV-1 AMG). Besides typical nucleosomal (i) chromatin, thin (3-5 nm) strands morphologically indistinguishable from prote...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Müller U,Schröder CH,Zentgraf H,Franke WW

    更新日期:1980-12-01 00:00:00

  • Identification of a complex between alpha-actinin and the intermediate filament subunit skeletin in bovine heart Purkinje fibres.

    abstract::A three-dimensional cytoskeleton, morphologically composed of intermediate filaments, desmosomes, and Z-disks, remains in bovine heart Purkinje fibres after extraction with Triton X-100 and low and high ionic strength solutions. Biochemically this cytoskeleton mainly consists of skeletin (Mr approximately 55 000) and ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Kjörell U,Thornell LE

    更新日期:1982-08-01 00:00:00

  • Coated pits and pinocytosis of cationized ferritin in human skin fibroblasts.

    abstract::Pinocytosis was studied in cultured human skin fibroblasts in order to obtain more information about uptake in these cells of non-specific ligands with different net surface charge. The fibroblasts were obtained from a normal person and a patient homozygous for familial hypercholesterolemia. No ultrastructural differe...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: van Deurs B,Nilausen K,Faergeman O,Meinertz H

    更新日期:1982-06-01 00:00:00

  • Expression pattern of Drosophila translin and behavioral analyses of the mutant.

    abstract::Translin is an evolutionarily conserved approximately 27-kDa protein that binds to specific DNA and RNA sequences and has diverse cellular functions. Here, we report the cloning and characterization of the translin orthologue from the fruit fly Drosophila melanogaster. Under protein-denaturing conditions, purified Dro...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2006.11.005

    authors: Suseendranathan K,Sengupta K,Rikhy R,D'Souza JS,Kokkanti M,Kulkarni MG,Kamdar R,Changede R,Sinha R,Subramanian L,Singh K,Rodrigues V,Rao BJ

    更新日期:2007-03-01 00:00:00

  • Cell adhesion-dependent regulation of cell growth during sea urchin development.

    abstract::In the sea urchin embryo there are at least two cell adhesion molecules related to mammalian cadherins, one of them, similar to E-cadherin, is expressed in embryos at very early developmental stages, the second appears at the blastula stage (G. Ghersi et al. Mech. Dev. 41, 47-55 (1993)). We show here that when sea urc...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Ghersi G,Salamone M,Levi G,Vittorelli ML

    更新日期:1996-03-01 00:00:00

  • Distribution of sulfated glycosaminoglycans in the glomerular basement membrane and mesangial matrix.

    abstract::The relative distribution of heparan sulfate-glycosaminoglycan (HS-GAG) and chondroitin sulfate-glycosaminoglycans (CS-GAG) of the mesangial matrix (MM) and the glomerular basement membrane (GBM), which represent the two glomerular extracellular matrices, was determined by a combination of enzymatic treatments and aut...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Kanwar YS,Jakubowski ML,Rosenzweig LJ

    更新日期:1983-09-01 00:00:00

  • The spatial and temporal expression of Tekt1, a mouse tektin C homologue, during spermatogenesis suggest that it is involved in the development of the sperm tail basal body and axoneme.

    abstract::Tektins comprise a family of filament-forming proteins that are known to be coassembled with tubulins to form ciliary and flagellar microtubules. Recently we described the sequence of the first mammalian tektin protein, Tekt1 (from mouse testis), which is most homologous with sea urchin tektin C. We have now investiga...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00097

    authors: Larsson M,Norrander J,Gräslund S,Brundell E,Linck R,Ståhl S,Höög C

    更新日期:2000-10-01 00:00:00

  • The division of pleomorphic plastids with multiple FtsZ rings in tobacco BY-2 cells.

    abstract::Plastids, an essential group of plant cellular organelles, proliferate by division to maintain continuity through cell lineages in plants. In recent years, it was revealed that the bacterial cell division protein FtsZ is encoded in the nuclear genome of plant cells, and plays a major role in the plastid division proce...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00318

    authors: Momoyama Y,Miyazawa Y,Miyagishima SY,Mori T,Misumi O,Kuroiwa H,Tsuneyoshi K

    更新日期:2003-06-01 00:00:00

  • Cysteine proteinases in GH4C1 cells, a rat pituitary tumor cell line, are secreted by the constitutive and regulated secretory pathways.

    abstract::Secretory granules of GH4C1 cells, a rat pituitary tumor cell line, are known to be induced by the treatment of estradiol (E2), insulin, and epidermal growth factor (EGF). We examined changes in the localization of cathepsins B, H, and L, lysosomal cysteine proteinases, in GH4C1 cells before and after hormonal treatme...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Waguri S,Sato N,Watanabe T,Ishidoh K,Kominami E,Sato K,Uchiyama Y

    更新日期:1995-08-01 00:00:00

  • Centromere regulation: new players, new rules, new questions.

    abstract::Centromeres support the assembly of the kinetochore on every chromosome and are therefore essential for the proper segregation of sister chromatids during cell division. Centromere identity is regulated epigenetically through the presence of the histone H3 variant CENP-A. CENP-A regulation and incorporation specifical...

    journal_title:European journal of cell biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ejcb.2011.04.016

    authors: Pauleau AL,Erhardt S

    更新日期:2011-10-01 00:00:00

  • A nuclear lamin of the nematode Caenorhabditis elegans with unusual structural features; cDNA cloning and gene organization.

    abstract::This report describes the characterization of the nuclear lamin CeLam-1 of the nematode Caenorhabditis elegans by molecular analysis of the corresponding complete cDNA and gene sequences. The primary structure of CeLam-1, representing only the third non-vertebrate lamin sequence currently known, follows essentially th...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Riemer D,Dodemont H,Weber K

    更新日期:1993-12-01 00:00:00

  • Electron microscopic characterization of intermediate filaments in rat liver parenchymal cells.

    abstract::Filamentous networks were isolated from rat liver and studied by electron microscopy after negative staining with uranyl acetate. Highly purified preparations of networks were used to obtain material for immunological studies, but morphological studies were made more satisfactorily on preparations purified only by suc...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Jahn W

    更新日期:1982-02-01 00:00:00

  • Crumbs regulates polarity and prevents light-induced degeneration of the simple eyes of Drosophila, the ocelli.

    abstract::The evolutionary conserved transmembrane protein Crumbs (Crb) regulates morphogenesis of photoreceptor cells in the compound eye of Drosophila and prevents light-dependent retinal degeneration. Here we examine the role of Crb in the ocelli, the simple eyes of Drosophila. We show that Crb is expressed in ocellar photor...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2012.03.006

    authors: Mishra M,Rentsch M,Knust E

    更新日期:2012-09-01 00:00:00

  • Acetylation of translationally controlled tumor protein promotes its degradation through chaperone-mediated autophagy.

    abstract::Translationally controlled tumor protein (Tpt1/TCTP) is a multi-functional cytosolic protein whose cellular levels are finely tuned. TCTP regulates protein behavior by favoring stabilization of protein partners or on the contrary by promoting degradation of others. TCTP has been shown to be transcriptionally and trans...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2016.12.002

    authors: Bonhoure A,Vallentin A,Martin M,Senff-Ribeiro A,Amson R,Telerman A,Vidal M

    更新日期:2017-03-01 00:00:00

  • Carbonic anhydrase XII promotes invasion and migration ability of MDA-MB-231 breast cancer cells through the p38 MAPK signaling pathway.

    abstract::Carbonic anhydrase (CA) XII, an extracellular enzyme involved in the regulation of the microenvironment acidity and tumor malignant phenotype, was originally identified as a protein overexpressed in some types of cancers, including breast cancer. However, the cellular function and mechanism of CAXII remained unclear. ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2010.03.004

    authors: Hsieh MJ,Chen KS,Chiou HL,Hsieh YS

    更新日期:2010-08-01 00:00:00

  • Differential contribution of syntaxin 1 and SNAP-25 to secretion in noradrenergic and adrenergic chromaffin cells.

    abstract::We used botulinum neurotoxins (BoNT) to examine whether differences in the secretory activity of noradrenergic and adrenergic chromaffin cells are related to differences in the exocytotic machinery of these two types of bovine adrenal medulla cells. Cleavage of syntaxin and SNAP-25 by BoNT/C1 decreased in a dose-depen...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00128

    authors: Baltazar G,Tomé A,Carvalho AP,Duarte EP

    更新日期:2000-12-01 00:00:00

  • Expression and immunolocalization of the aquaporin-8 water channel in rat gastrointestinal tract.

    abstract::A remarkable amount, of water is transported in the gastrointestinal (GI) organs to fulfil the secretory and absorptive functions of the GI tract. However, the molecular basis of water movement in the GI epithelial barriers is still poorly known. Important clues about the mechanisms by which water is transported in th...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00210

    authors: Calamita G,Mazzone A,Bizzoca A,Cavalier A,Cassano G,Thomas D,Svelto M

    更新日期:2001-11-01 00:00:00

  • Inactivation of p120 catenin in mice disturbs intrahepatic bile duct development and aggravates liver carcinogenesis.

    abstract::p120 catenin (p120ctn) is required for the stability of classic cadherins at the cell surface and is thought to play a central role in modulating cell-cell adhesion. Cytoplasmic p120ctn promotes cell motility, and probably other activities, by modulating the activities of RhoA, Rac and Cdc42. E-cadherin is expressed i...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2016.10.003

    authors: van Hengel J,Van den Broeke C,Pieters T,Libbrecht L,Hofmann I,van Roy F

    更新日期:2016-12-01 00:00:00

  • The cancer/testis antigen CAGE-1 is a component of the acrosome of spermatids and spermatozoa.

    abstract::Cancer/testis antigens (CTAs) are characterized by their restricted expression pattern. In normal individuals their expression is largely restricted to the testis. In the case of cancer patients, CTA expression has also been frequently observed in the tumoral cells. CTAs are considered to be promising targets for immu...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2004.11.003

    authors: Alsheimer M,Drewes T,Schütz W,Benavente R

    更新日期:2005-03-01 00:00:00

  • Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly.

    abstract::We have investigated the effect of stepwise truncating the carboxyterminal domain ("tail") of the intermediate filament (IF) protein vimentin of the clawed toad, Xenopus laevis, on filament assembly in vitro and, using cell transfection, in vivo and also on the cellular topology of the structures formed. All truncatio...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Rogers KR,Eckelt A,Nimmrich V,Janssen KP,Schliwa M,Herrmann H,Franke WW

    更新日期:1995-02-01 00:00:00

  • Analysis of gap junctional intercellular communications using a dielectrophoresis-based microchip.

    abstract::Gap junctions are transmembrane structures that directly connect the cytoplasm of adjacent cells, making intercellular communications possible. It has been shown that the behaviour of several tumours - such as bone tumours - is related to gap junction intercellular communications (GJIC). Several methodologies are avai...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2017.01.003

    authors: Tellez-Gabriel M,Charrier C,Brounais-Le Royer B,Mullard M,Brown HK,Verrecchia F,Heymann D

    更新日期:2017-03-01 00:00:00

  • Characterization of the TGN exit routes in AtT20 cells using pancreatic amylase and serum albumin.

    abstract::The AtT20 pituitary cell is the one that was originally used to define the pathways taken by secretory proteins in mammalian cells. It possesses two secretory pathways, the constitutive for immediate secretion and the regulated for accumulation and release under hormonal stimulation. It is in the regulated pathway, mo...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00365

    authors: Daull P,Home W,LeBel D

    更新日期:2004-04-01 00:00:00

  • P446L-importin-beta inhibits nuclear envelope assembly by sequestering nuclear envelope assembly factors to the microtubules.

    abstract::The P446L mutant Drosophila importin-beta (P446L-imp-beta) has been reported to prohibit--in dominant negative fashion--nuclear envelope (NE) assembly. Along elucidating the mode of action of P446L-imp-beta we studied in vitro NE assembly on Sepharose beads. While Drosophila embryo extracts support NE assembly over Se...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00324

    authors: Tirián L,Timinszky G,Szabad J

    更新日期:2003-07-01 00:00:00

  • The coincident distribution of calcium-rich membranes and kinetochore fibers at metaphase in living endosperm cells of Haemanthus.

    abstract::We have coupled chlorotetracycline (CTC) fluorescence with polarized light microscopic observations of living mitotic endosperm cells of Haemanthus in order to compare the spatial distributions of Ca2+-rich endomembranes in the spindle with that of the kinetochore fibers during metaphase. In either control cells or in...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Wolniak SM,Hepler PK,Jackson WT

    更新日期:1981-08-01 00:00:00

  • The minimal ESCRT machinery of Giardia lamblia has altered inter-subunit interactions within the ESCRT-II and ESCRT-III complexes.

    abstract::The ESCRT pathway functions at different subcellular membranes to induce their negative curvature, and it has been largely characterized in model eukaryotes belonging to Opisthokonta. But searches of the genomes of many nonopisthokonts belonging to various supergroups indicate that some of them may harbour fewer ESCRT...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2017.11.004

    authors: Saha N,Dutta S,Datta SP,Sarkar S

    更新日期:2018-01-01 00:00:00