Abstract：
:The plasma membrane of nucleated erythrocytes contains a microtubular marginal band which appears to be associated with the plasma membrane skeleton. In this report, we identify two families of cytoskeletal proteins which may be involved in such an association. These proteins, of molecular mass 78 kDa and 48 kDa on SDS-PAGE, are shown to bind tubulin based on a 125I-labeled tubulin binding assay. Solubilization of isolated chicken erythrocyte plasma membranes in Triton X-100 shows that these proteins centrifuge with the pellet, indicating that they are bound to the membrane skeleton. Finally, immunofluorescence studies using antisera raised against the 78 kDa and 48 kDa proteins show that they colocalize with the marginal band in intact cells. Colocalization of cytoskeletal tubulin-binding proteins with the marginal band favors a hypothesis suggesting that the 78 kDa and 48 kDa proteins are involved in the association of the two molecular superstructures.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Stetzkowski-Marden F,Deprette C,Cassoly Rsubject
Has Abstractpub_date
1991-02-01 00:00:00pages
102-9issue
1eissn
0171-9335issn
1618-1298journal_volume
54pub_type
杂志文章abstract：:Previously we reported the novel observation that astrocytes ensheath the persistent hyaloid artery, both in the Nuc1 spontaneous mutant rat, and in human PFV (persistent fetal vasculature) disease (Developmental Dynamics 234:36-47, 2005). We now show that astrocytes isolated from both the optic nerve and retina of Nu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2011.01.003
更新日期：2011-05-01 00:00:00
abstract：:In the sea urchin embryo there are at least two cell adhesion molecules related to mammalian cadherins, one of them, similar to E-cadherin, is expressed in embryos at very early developmental stages, the second appears at the blastula stage (G. Ghersi et al. Mech. Dev. 41, 47-55 (1993)). We show here that when sea urc...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：:Cultured fetal rat hepatocytes derived from 12, 15 and 19-day gestation rats are capable of secreting transferrin. When dexamethasone is added to the medium an increased secretion rate is observed. The changes in secretion rates in control as well as dexamethasone-treated cells during culture have been shown to correl...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:The ESCRT pathway functions at different subcellular membranes to induce their negative curvature, and it has been largely characterized in model eukaryotes belonging to Opisthokonta. But searches of the genomes of many nonopisthokonts belonging to various supergroups indicate that some of them may harbour fewer ESCRT...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2017.11.004
更新日期：2018-01-01 00:00:00
abstract：:Previous studies have shown that caffeine treatment at 20 degrees C causes the intermediate compartment protein p58 to redistribute from the Golgi region without affecting the localization of the Golgi stack protein mannosidase II (J. Jäntti, E. Kuismanen, J. Cell Biol. 120, 1321-1335 (1993). Here we have dissected fu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:The molecular clutch (MC) model proposes that actomyosin-driven force transmission permits integrin-dependent cell migration. To investigate the MC, we introduced diverse talin (TLN) and integrin variants into Flp-In™ T-Rex™ HEK293 cells stably expressing uPAR. Vitronectin variants served as substrate providing uPAR-m...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2015.10.002
更新日期：2016-01-01 00:00:00
abstract：:When virgin temperature-sensitive mutant cdc28 cells of the yeast Saccharomyces cerevisiae were incubated at restrictive temperatures, they continued to grow, reaching a maximum of 3.3-fold the original size after 24 h. The protein and RNA levels increased during the first 24 h, then gradually decreased. The cells tha...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:The presence of a filamin-like protein in oxyntic cells was established by indirect immunofluorescence microscopy. The location of this protein and myosin was studied, using specific antibodies, on frozen sections and isolated cells. Antifilamin and antimyosin reacted strongly with the luminal cytoplasm of the cells. ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:Mechanical tension governs fibroblast proliferation and survival and the homeostasis of the extracellular matrix to adapt its resistance to the mechanical requirements of the organs. To consolidate this view, we analysed the effect of tension release on the expression of molecules involved in the architecture and stab...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00181
更新日期：2001-07-01 00:00:00
abstract：:The development of an efficient immune response depends on the capacity of antigen-specific lymphocytes to migrate into secondary lymphoid organs. The first step in the process of lymphocyte extravasation involves lymphocyte binding to the vascular endothelium. Although several adhesion receptors have been implicated ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:Embryonic chick myoblasts possess an extensive network of cytoplasmic microtubules which emanate from a single, perinuclear centrosome containing a microtubule-organizing center (MTOC) and the centrioles. However, after myoblasts fuse into myotubes the centrosome is no longer apparent, and instead long parallel arrays...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Oocyte-thymocyte mouse cell hybrids were produced using polyethylene glycol (PEG) and examined at the ultrastructural level. Fusion was accomplished either before or after activation of metaphase II oocytes. In both experimental variants thymocyte nuclei undergo remodelling which comprises the following sequence of ev...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:The v-mos oncogene product has the ability to induce differentiation in human monocytic leukemia U937 cells, thereby arresting cell proliferation, and also exhibits transforming activity in mouse NIH3T3 cells. Mutation in the v-mos gene consisting of one or two amino acid substitutions in the putative ATP-binding doma...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Specific antibodies to a protein designated P1 (Mr approximately equal to 63,000), which is specifically altered in mutants resistant to the microtubule inhibitor podophyllotoxin, bind to mitochondria in cells of various vertebrate and invertebrate species (Eur. J. Cell Biol. 44, 278-285 (1987); Can. J. Biochem. Cell ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
abstract：:The 90-kDa heat-shock protein, hsp90, is an abundant and conserved protein located predominantly in the cytoplasm. Previous reports have localized a portion of the hsp90 to microtubules and to microfilaments. Here, we show that colocalization of hsp90 with microtubules is seen after long-term but not short-term fixati...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:High-speed centrifugation supernatants from slime mould plasmodia show considerable activities to inhibit the polymerization of actin as revealed by viscosity measurements. By following increasing inhibitory activities an actin modulating protein (AM-protein) has been isolated and purified which affects the polymer st...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:D-amino acid oxidase is expressed to a high level in the yeast Rhodotorula gracilis (0.3% of total cell protein) through induction by D-alanine in a defined growth medium. Monospecific polyclonal antibodies against pure enzyme were obtained. Western blot analysis showed that the enzyme is synthesized as the mature pol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:To investigate the association of calmodulin (CaM) with microtubules (MTs) in the mitotic apparatus (MA), the distributions of both CaM and tubulin were examined in mitotic PtK1 cells in which MT subclasses had been selectively removed or altered by treatment with cold or with the MT inhibitor, nocodazole. A fluoresce...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:The intracellular storage of zinc in Malpighian tubules of Drosophila hydei was studied by X-ray microanalysis of freeze-dried cryosections. Mass dense vacuoles in the proximal region of the anterior larval Malpighian tubule cells were found to accumulate zinc, not sodium. The zinc content was enhanced considerably af...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Actin, myosin, and the actin-associated proteins tropomyosin, alpha-actinin, vinculin, and villin were localized in acinar cells of rat and bovine pancreas, parotid, and prostate glands by means of immunofluorescent staining of both frozen tissue sections and semithin sections of quick-frozen, freeze-dried, and plasti...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Initial integrin-mediated cell-matrix adhesions (focal complexes) appear underneath the lamellipodia, in the regions of the "fast" centripetal flow driven by actin polymerization. Once formed, these adhesions convert the flow behind them into a "slow", myosin II-driven mode. Some focal complexes then turn into elongat...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2005.11.001
更新日期：2006-04-01 00:00:00
abstract：:The fine structure of a joint receptor (R10) in a spider leg (Zygiella x-notata) was examined with light and electron microscopy. The R10 receptor consists of a compact ganglion which is situated near the dorsal joint membrane of the femur/patella joint. Each of the ten sensory cells comprising the ganglion sends one ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:A great body of data increasingly point to the cell membrane as an important target for adriamycin (ADR). However, the exact mechanism by which ADR exerts its cytotoxic action through the interaction with the plasma membrane is still unknown. In this study, the interaction of ADR with red blood cells from healthy dono...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:Triton-treated cells of the coenocytic green alga, Ernodesmis verticillata, manifest longitudinal contraction and centripetal closure of the cytoplasm in the presence of exogenous free calcium ions. Complete closure of the cytoplasm around the vacuolar remnants is obtained with 1 mM ATP present in the activation mediu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:The absence of nucleosome-like structures from purified nuclei of the primitive dinoflagellate Prorocentrum micans was demonstrated by three means. i) Electron microscopy revealed mostly thin, smooth 6-nm nucleofilaments in chromatin incubated at various ionic strengths and either fixed in 0.1% glutaraldehyde or unfix...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:In response to hypotonic stress, cells of the inner medullary collecting duct (IMCD) undergo swelling followed by a regulatory volume decrease (RVD) and a transient release of organic osmolytes such as sorbitol. In this study, we tested the hypothesis whether membrane recycling is involved in the latter process. There...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:MUC16/CA125 is over-expressed in human epithelial tumors including ovarian, breast and some other carcinomas. The purpose of this study is to investigate how cell surface MUC16 is functionally involved in tumor progression, with a special focus on the role of its cytoplasmic tail. Forced expression of C-terminal MUC16...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2013.10.005
更新日期：2013-08-01 00:00:00
abstract：:The interaction between myelin basic protein (MBP) and G-actin was studied under nonpolymerizing conditions, i.e.,2mM HEPES, pH 7.5, 0.1 mM CaCl2 and 0.2 mM ATP. Fluorescence studies using pyrenyl-actin and the measurements of ATP hydrolysis rate show that MBP induces changes in the structure of the actin monomer simi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Channels of the surface-connected, open canalicular system (OCS) of human platelets serve as the pathway for transport of substances into the cells and as conduits for the discharge of alpha granule products secreted during the platelet release reaction. The purpose of the present study was to determine if both functi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:In solid body tissues, environmental biomechanics is indispensable for tissue homeostasis. While characteristics of homeostasis include morphogenesis, proliferation and differentiation, the influences through biomechanics in corneal keratinocytes are poorly understood. Here we show for the first time that corneal kera...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2011.08.005
更新日期：2011-12-01 00:00:00
