Abstract:
:Actin, myosin, and the actin-associated proteins tropomyosin, alpha-actinin, vinculin, and villin were localized in acinar cells of rat and bovine pancreas, parotid, and prostate glands by means of immunofluorescent staining of both frozen tissue sections and semithin sections of quick-frozen, freeze-dried, and plastic-embedded tissues. Antibodies to actin, myosin, tropomyosin, alpha-actinin, and villin reacted strongly with a narrow cytoplasmic band extending beneath the luminal border of acinar cells. The presence of villin, which has so far been demonstrated only in intestinal and kidney brush border, was further confirmed by antibody staining of blotted electrophoresis gels of whole acinar cell extracts. Fluorescently labelled phalloidin, which reacts specifically with F-actin, gave similar staining, within the cell apex to that obtained with antibodies to actin, myosin, tropomyosin, alpha-actinin, and villin. In contrast, immunostaining with antibodies to vinculin was restricted to the area of the junctional complex. Ultrastructurally, the apical immunoreactive band corresponded to a dense web composed of interwoven microfilaments, which could be decorated with heavy meromyosin. Outside this apical terminal web, antibodies to myosin and tropomyosin gave only a weak immunostaining (confined to the lateral cell borders) whereas antibodies to actin and alpha-actinin led to a rather strong bead-like staining along the lateral and basal cell membrane most probably marking microfilament-associated desmosomes. Anti-villin immunofluorescence was confined to the apical terminal web. It is suggested that the apical terminal web is important for the control of transport and access of secretory granules to the luminal plasma membrane and that villin, which is known to bundle or sever actin filaments in a Ca(++)-dependent manner, might participate in the regulation of actin polymerization within this strategically located network of contractile proteins.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Drenckhahn D,Mannherz HGsubject
Has Abstractpub_date
1983-05-01 00:00:00pages
167-76issue
2eissn
0171-9335issn
1618-1298journal_volume
30pub_type
杂志文章abstract::Hydrogencarbonate and chloride activated, ouabain-insensitive ATPase activities are demonstrated in the salt-absorbing rectum of larval dragonflies. Maximal activation is achieved at approx. 30 mM HCO3- and 20 mM Cl-, respectively. The stimulation of each anion obeys Michaelis-Menten kinetics Km values are 4.65 mM for...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-02-01 00:00:00
abstract::In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequen...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::Neurofilament (NF) structure and ability to form cross-bridges were examined by quick-freeze deep-etch mica and low-angle rotary-shadow electron microscopy in NFs purified from bovine spinal cord and reassembled in various combinations of NF subunits. When NFs were reassembled from triplet proteins, NF-L, NF-M and NF-...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::Several different protein families were shown to be involved in the regulation of actin filament formation and have been studied extensively in processes such as cell migration. Among them are members of the formin family, which tend to promote the formation of linear actin filaments. Studies in recent years, often us...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2013.11.004
更新日期:2014-05-01 00:00:00
abstract::The 90-kDa heat-shock protein, hsp90, is an abundant and conserved protein located predominantly in the cytoplasm. Previous reports have localized a portion of the hsp90 to microtubules and to microfilaments. Here, we show that colocalization of hsp90 with microtubules is seen after long-term but not short-term fixati...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::Carbonic anhydrase (CA) XII, an extracellular enzyme involved in the regulation of the microenvironment acidity and tumor malignant phenotype, was originally identified as a protein overexpressed in some types of cancers, including breast cancer. However, the cellular function and mechanism of CAXII remained unclear. ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2010.03.004
更新日期:2010-08-01 00:00:00
abstract::1. Feeding weanling mice a diet containing 0.5% (w/w) cuprizone (biscyclohexanone oxaldihydrazone) induced megamitochondria of a different type of that previously described (Suzuki, K., Science 163, 81-82 (1969). 2. As in the normal controls, a single population of mitochondria with a narrow size distribution, was det...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::In the present study the role of endothelin (ET) and its receptors (ETA-R and ETB-R) in cellular mechanisms underlying the resistance of astroglial cells to low oxygen level and development of hypoxia has been investigated. To define the influences of ET and its receptors on survival and on antigenic as well as morpho...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2004.12.030
更新日期:2005-06-01 00:00:00
abstract::Signal transduction elements, including protein kinase C, have been identified in mammalian spermatozoa. In order to evaluate the pattern of expression and the subcellular localization of nine different protein kinase C isoforms in the course of spermatogenesis, we utilized quantitative electron microscopy immunocytoc...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::The nucleolar protein B-36 is an RNA-associated protein which has a number of properties in common with pre-mRNA-binding proteins (hnRNP proteins). Like the hnRNP proteins, B-36 appears to be evolutionarily conserved among various eukaryotes (protists and several animal species). The conservation of B-36 throughout th...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::Exocytosis of synaptic vesicles (SV) results in the surface exposure of lumenal epitopes of SV proteins. We have recently described the use of antibodies directed against the lumenal N-terminus of synaptotagmin I (Sytlum-Abs) to morphologically monitor exo-endocytic recycling of SVs. We report here that a radioimmunoa...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
abstract::Zymogen granules (ZG) of rat pancreas have been isolated by the procedure of Pâquet et al. The granules lysed when exposed to alkaline pH (pH 8.2), and their membranes could be subfractionated by centrifugation on a sucrose gradient. Four discrete types of membranes corresponding to densities of 1.105, 1.085, 1.075, a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::Proteins that are concentrated in specific compartments of the endomembrane system in order to exert their organelle-specific function must possess specific localization signals that prevent their transport to distal regions of the exocytic pathway. Some resident proteins of the endoplasmic reticulum (ER) that are kno...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70025-4
更新日期:2000-04-01 00:00:00
abstract::As proposed by Hanahan and Weinberg (2000. Cell 100, 57-70) carcinogenesis requires crucial events such as (i) genomic instability, (ii) cell cycle deregulation, (iii) induction of a telomere length maintenance mechanism, and (iv) an angiogenic switch. By comparing the expression of p53, cyclin D1, p16, hTERT, and TSP...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2006.11.002
更新日期:2007-12-01 00:00:00
abstract::The importance of intact microtubules in the processing, storage and regulated secretion of von Willebrand factor (vWf) from Weibel-Palade bodies in endothelial cells was investigated. Human umbilical vein endothelial cells treated for 1 h with colchicine (10(-6) M) or nocodozole (10(-6) M) lost their organized microt...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::PinX1 was originally identified as a Pin2/TRF1-interacting protein that suppresses telomerase activity via its telomerase inhibitor domain (TID) and regulates the nucleolar localization of TRF1 in telomerase-positive cells. In addition to its telomeric localization, PinX1 can be found in the nucleoli of human cells. O...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2010.05.005
更新日期:2010-10-01 00:00:00
abstract::Three-dimensional collagen lattice cultures of fibroblasts mimic the in vivo situation better than monolayer cultures. Here, skin fibroblasts from scleroderma patients and healthy controls were cultivated in collagen lattices, and the effects of recombinant human gamma-interferon (IFN-gamma) on these cultures investig...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::When resting human blood platelets are stimulated with thrombin, 50 to 60% of the G-actin polymerizes to F-actin within 60 seconds. The increase in F-actin is correlated with a corresponding decrease in the complex of G-actin with T beta 4. Within 5 seconds after stimulation, nucleation sites for pyrene actin polymeri...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::mRNA translation is regulated by diverse mechanisms that converge at the initiation and elongation steps to determine the rate, profile, and localization of proteins synthesized. A consistently relevant feature of these mechanisms is the spatial re-distribution of translation machinery, a process of particular importa...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2018.02.002
更新日期:2018-04-01 00:00:00
abstract::The release of newly synthesized neuropeptides was studied in an in vitro system using the adipokinetic hormone (AKH)-producing cells of an insect (Locusta migratoria) as a model system. Tritiated phenylalanine incorporated into three hormonal neuropeptides, AKH I, II and III, was used to distinguish newly synthesized...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::A great body of data increasingly point to the cell membrane as an important target for adriamycin (ADR). However, the exact mechanism by which ADR exerts its cytotoxic action through the interaction with the plasma membrane is still unknown. In this study, the interaction of ADR with red blood cells from healthy dono...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::In this study we have used primary cultures of porcine superior cervical ganglia as a model system to study exo-endocytosis in sympathetic neurons. Pure neuronal cultures with a defined noradrenergic phenotype can be obtained when antimitotics are included in the culture medium, and the high yield from prenatal piglet...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::Human peripheral blood monocytes were obtained in very pure preparations by using buoyant density gradient centrifugation followed by velocity sedimentation. We introduced several modifications in the experimental procedure in order to take advantage of the minimal differences in cell density and size between monocyte...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::We have reported previously that the human promyelocytic leukemia cell line HL-60, in its undifferentiated state, is devoid of cytoplasmic intermediate filament proteins and nuclear lamins A and C, but does express lamin B. Using immunofluorescence and immunoblotting techniques, we have further investigated the expres...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::Initial integrin-mediated cell-matrix adhesions (focal complexes) appear underneath the lamellipodia, in the regions of the "fast" centripetal flow driven by actin polymerization. Once formed, these adhesions convert the flow behind them into a "slow", myosin II-driven mode. Some focal complexes then turn into elongat...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2005.11.001
更新日期:2006-04-01 00:00:00
abstract::Mouse plasma cells have been exposed to a wide range of soluble and adsorptive macromolecular tracers for 10 min to 4 h to explore the possibility of membrane recycling related to the high secretory rate of these nonregulated secretory cells. Electron microscopic examination showed in all cases labeling of multivesicu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::Interleukin-1 beta (Il-1 beta) and interleukin-1 alpha (Il-1 alpha) were shown to act as motility factors for the human breast carcinoma cell lines SK-BR-3 and ZR-75-1 in vitro. Both cytokines induced transition from the stationary to the motile phenotype (spreading). Il-1 beta stimulated translocation, shape change a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::Gap junctions are transmembrane structures that directly connect the cytoplasm of adjacent cells, making intercellular communications possible. It has been shown that the behaviour of several tumours - such as bone tumours - is related to gap junction intercellular communications (GJIC). Several methodologies are avai...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.01.003
更新日期:2017-03-01 00:00:00
abstract::Na/K-ATPase of salt-stressed salt glands of the domestic duck (Anas platyrhynchos) was purified in membrane-bound form by incubation of the microsomal fraction with sodium dodecylsulphate and ATP followed by discontinuous sucrose gradient centrifugation. Gel electrophoresis of the purified plasma membrane preparation ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::We examined the Ca2+ handling property and cell function of CCL39 fibroblasts highly overexpressing the cardiac isoform (NCX1) of Na+/ Ca2+ exchanger. In NCX1 transfectants in 146 mM Na+, ionomycin, alpha-thrombin or thapsigargin only produced a small transient increase in [Ca2+]i compared to the large increase seen i...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80038-1
更新日期:1998-07-01 00:00:00