Abstract：
:Specific antibodies to a protein designated P1 (Mr approximately equal to 63,000), which is specifically altered in mutants resistant to the microtubule inhibitor podophyllotoxin, bind to mitochondria in cells of various vertebrate and invertebrate species (Eur. J. Cell Biol. 44, 278-285 (1987); Can. J. Biochem. Cell Biol. 63, 489-502 (1985)). To investigate the relationship of this protein to mitochondria, rat liver mitochondria have been purified and immunoblot analysis with these provide evidence that the P1 protein is a major component of mitochondria. Two-dimensional gel electrophoretic analysis of mitochondrial proteins from Chinese hamster ovary (CHO) cells also show the P1 protein to be a major mitochondrial component. Subfractionation of rat liver mitochondria into various compartments indicates that the P1 protein is mainly associated with the matrix fraction. Effect of treatment of CHO cells with mitochondrial inhibitors on the synthesis of P1 protein was also investigated. Treatment with the K+ ionophores nonactin and valinomycin, which abolish mitochondrial membrane potential, inhibited synthesis of the mature forms of the P1 protein as well as a number of other mitochondrial proteins, as seen by two-dimensional gel electrophoresis of labeled polypeptides. Treatment of the podophyllotoxin-resistant mutant of CHO cells with the above inhibitors affected both the wild-type and the mutant forms of the P1 protein in a similar manner. Concomitant with the disappearance of the above proteins, new basic proteins of higher molecular masses, related to the P1 and other proteins by peptide analysis, were observed in the drug-treated cells.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Gupta RS,Austin RCsubject
Has Abstractpub_date
1987-12-01 00:00:00pages
170-6issue
1eissn
0171-9335issn
1618-1298journal_volume
45pub_type
杂志文章abstract：:Triton-treated cells of the coenocytic green alga, Ernodesmis verticillata, manifest longitudinal contraction and centripetal closure of the cytoplasm in the presence of exogenous free calcium ions. Complete closure of the cytoplasm around the vacuolar remnants is obtained with 1 mM ATP present in the activation mediu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:The milk yeast Kluyveromyces lactis is an alternative model yeast to the well established Saccharomyces cerevisiae. The cell wall of these fungi consists of polysaccharides (i.e. long chains of β-1,3- and β-1,6-linked sugar chains and some chitin) and mannoproteins, both of which are continually adapted to environment...
journal_title：European journal of cell biology
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abstract：:It has only recently been recognized that intermediate filaments (IFs) and their assembly intermediates are highly motile cytoskeletal components with cell-type- and isotype-specific characteristics. To elucidate the cell-type-independent contribution of actin filaments and microtubules to these motile properties, flu...
journal_title：European journal of cell biology
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abstract：:Hepatocytoma (HPCT) hybrid cells were obtained by fusion of cultured rat hepatocytes with Fao Reuber hepatoma cells H35 by polyethylene glycol treatment. Surviving cells were cloned in HAT (hypoxanthine-aminopterine-thymidine)/ouabain medium and propagated in cell lines over 80 passages. Morphological criteria were ch...
journal_title：European journal of cell biology
pub_type： 杂志文章
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更新日期：1994-08-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1992-04-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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更新日期：2000-12-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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更新日期：1999-11-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1981-12-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1992-02-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章,评审
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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更新日期：2001-11-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1991-12-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1996-02-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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doi：
更新日期：1983-03-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1998-04-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
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更新日期：2000-11-01 00:00:00
