Abstract：
:In an attempt to characterize connexin genes expressed early in mouse development we screened a cDNA library from mouse F9 embryonic carcinoma cells with mouse connexin37 cDNA and mouse connexin31.1 genomic DNA under low stringency of hybridization. We detected 5 different connexin cDNAs coding for mouse connexins31, -31.1, -32, -43, and -45 (reviewed in Willecke et al., Eur. J. Cell Biol. 56, 1-7 (1991)). Here we describe characterization of mouse connexin31 cDNA coding for a protein of 270 amino acids (Mr 30,905) that shows 8 amino acid exchanges compared to its rat analog recently deduced from its genomic sequence. Mouse connexin45 cDNA codes for a protein of 396 amino acids (Mr 45,671) that exhibits 84% amino acid identity compared to its chick analog described. Cx31 and Cx45 are coded for by single genes in the mouse genome. After Northern blot hybridization, we detected two Cx31 transcripts of 1.9 and 2.3 kb in total mouse RNA from skin, keratinocyte-derived cell lines, and in testis. Cx45 cDNA hybridized to a 2.2 kb mRNA in lung, brain, skin, heart, and intestine. This transcript showed maximal expression in adult lung and in embryonic tissues tested (brain, skin, kidney) where it was at least 40-fold more abundant than in the corresponding adult tissues.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Hennemann H,Schwarz HJ,Willecke Ksubject
Has Abstractpub_date
1992-02-01 00:00:00pages
51-8issue
1eissn
0171-9335issn
1618-1298journal_volume
57pub_type
杂志文章abstract：:Seminal roots are initiated at the scutellar node during maize (Zea mays L.) embryo development. The maize mutant rtcs (rootless concerning crown and seminal roots) does not initiate seminal roots while its wild-type siblings form on average 2.9 seminal roots per seedling. In this study, proteome profiles of 25-day-ol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2009.11.014
更新日期：2010-02-01 00:00:00
abstract：:The docking complex of peroxisomal matrix protein import is composed of PEX13 and PEX14 in all species analyzed so far, whereas only yeast appears to possess an additional component, PEX17. In this report we isolated PEX14 complexes of Neurospora crassa. Among the complex constituents, one protein designated as PEX33 ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.07.003
更新日期：2010-12-01 00:00:00
abstract：:Tight junctions (TJ) of the fascia occludens type can be rapidly assembled in the human colon adenocarcinoma cell line HT 29 under the influence of trypsin or ammonium sulfate. We have studied the influence of the metabolic inhibitors, dinitrophenol (DNP) and deoxyglucose (DG), on the induced formation of TJ in this c...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:Yeast dynamin, Vacuolar Protein Sorting 1 (Vps1), has been implicated in recycling traffic from the endosome to the trans-Golgi network (TGN). Previous research showed a genetic interaction of Vps1 with all components of the GARP tethering complex, which anchors vesicles at the late Golgi membrane. We used the yeast t...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2017.04.004
更新日期：2017-09-01 00:00:00
abstract：:In the present study, a systematic examination of the relative expression pattern of the nuclear retinoid receptors (RAR and RXR) in various liver cells was performed. Our data demonstrate that RXRalpha is the dominant receptor in all liver cells, and that RARbeta is also expressed at a high level in most cells. More ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80078-2
更新日期：1998-10-01 00:00:00
abstract：:The sites of calcium-dependent dibasic endoprotease (Kex2; yscF) activity have been ultracytochemically localized in exponential cultures of haploid (alpha) wild-type strain of Saccharomyces cerevisiae, in its pep4-3 mutant and in the kex2 mutant. The gently prefixed cells were thoroughly washed and incubated in a buf...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Mitotic PtK1 spindles were UV irradiated (285 nm) during metaphase and anaphase between the chromosomes and the pole. The irradiation, a rectangle measuring 1.4 x 5 microns parallel to the metaphase plate, severed between 90 and 100% of spindle microtubules (MTs) in the irradiated region. Changes in organization of MT...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Cells of Oocystis solitaria after treatment with inhibitors of a) microfibril orientation b) microfibril synthesis, and c) microfibril crystallization have been investigated with the freeze-fracturing technique. Changes in morphology of the E-face of the plasma membrane as a result of these treatments have been record...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
abstract：:We have investigated the effects of bafilomycin A1, a specific inhibitor of vacuolar type H(+)-ATPase, on the secretion and intracellular distribution of prolactin in cultured rat pituitary tumor cells (GH3 cells). Pulse-labeling the cells with L-[35S]methionine for 5 min and subsequently incubating in chase medium co...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:Conventional fixation with glutaraldehyde fixatives at pH 7.4 did not preserve/promote thick filaments in Bufo smooth muscle. However, if pH was lowered to 6.0, thick filaments were present and addition of dimethyl sulfoxide (DMSO) led to a greater number of thick filaments. Stretch alone had little effect on the pres...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:The subcellular distribution of a recently described neutral trimming mannosidase, the Man9-mannosidase, has been investigated by immunoelectron microscopy in pig hepatocytes. This enzyme processes asparagine-linked N-acetylglucosamine2 mannose9 oligosaccharides by specifically cleaving three of the four alpha 1,2-lin...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:Rho GTPases are ubiquitously expressed across the eukaryotes where they act as molecular switches participating in the regulation of many cellular processes. We present an inventory of proteins involved in Rho-regulated signaling pathways in Dictyostelium discoideum that have been identified in the completed genome se...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2006.04.011
更新日期：2006-09-01 00:00:00
abstract：:Previous work has shown that Trypanosoma cruzi extracellular amastigotes as well as metacyclic trypomastigotes infect cultured cells in a highly specific parasite form-cell type interaction. In this work we have investigated the mode of interaction of both forms with HeLa and Vero cells using scanning electron and con...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80093-4
更新日期：1999-12-01 00:00:00
abstract：:The milk yeast Kluyveromyces lactis is an alternative model yeast to the well established Saccharomyces cerevisiae. The cell wall of these fungi consists of polysaccharides (i.e. long chains of β-1,3- and β-1,6-linked sugar chains and some chitin) and mannoproteins, both of which are continually adapted to environment...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2011.04.005
更新日期：2011-09-01 00:00:00
abstract：:The monoclonal antibody MPM-12, raised by using partially purified extract of mitotic HeLa cells as the immunogen, preferentially stains the cytoplasm of mitotic cells by indirect immunofluorescence without exhibiting any species specificity. On immunoblots, MPM-12 recognizes three bands, of 155, 88, and 68 kDa, in mi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:Changes of the number and properties of the epidermal growth factor (EGF) receptor occur during liver regeneration and may be of importance in the maintenance of hepatocellular mass in liver cirrhosis. We therefore studied the changes in the number and distribution of EGF receptor in the development of liver cirrhosis...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:Changes in the surface morphology of regenerating rabbit, rat and frog corneal endothelial cells in vivo have been investigated by scanning electron microscopy. In adult tissue these cells do not normally divide unless given a stimulus, such as injury. Surfaces of quiescent rabbit and rat cells are devoid of microvill...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:The recently established in vitro assay of condensation-sorting of pancreatic enzymes to the zymogen granule membrane (ZGM) (Dartsch, H., R. Kleene, H. F. Kern: In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells. Eur. J. Cell Biol. 75, 211-222 (1998)) was used to study the invol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80009-0
更新日期：1999-02-01 00:00:00
abstract：:In the eye lens, three connexins have been detected in epithelial cells and bow region/differentiating fiber cells, suggesting the possible formation of heteromeric gap junction channels. To study possible interactions between Cx56 and Cx43, we stably transfected a normal rat kidney cell line (NRK) that expresses Cx43...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00132
更新日期：2001-01-01 00:00:00
abstract：:The induction of pollen embryogenesis in Capsicum annuum L. has been studied at the cellular level using various in situ approaches with several molecular probes for DNA, RNA and proteins. The late vacuolated microspore and the young bicellular pollen grain are stages of gametophytic development in which embryogenesis...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:While many studies of erythropoiesis have focused on interactions between developing cells and the extracellular matrix, scant attention has been paid to the role of erythroid-macrophage interactions in this process. Murine erythroleukemia (MEL) cell lines have been used extensively in these studies to define erythroi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:The spatial and temporal distribution as well as ultrastructural and biochemical characteristics of apoptotic and mitotic cells during human eye development were investigated in 14 human conceptuses of 4-9 postovulatory weeks, using electron and light microscopy. In the 5th developmental week, apoptotic and mitotic ce...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00328
更新日期：2003-08-01 00:00:00
abstract：:Embryonic stem (ES) cells are omnipotent; they can differentiate into every cell type of the body. The development of culture conditions that allow their differentiation has made it conceivable to produce large numbers of cells with lineage-specific characteristics in vitro. Here, we describe a method by which murine ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2007.07.001
更新日期：2007-12-01 00:00:00
abstract：:Differences in the carbohydrate composition were found in the jellies and the cortex of the unfertilized Xenopus laevis egg using lectins and blood group antibodies. Blood group H trisaccharide was detected in the outer jelly and "A-like" oligosaccharide was found in the inner jelly. The blood group A trisaccharide wa...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:RIP3 belongs to a group of recently identified proteins, classified as the ICR/RIP family whose members were described to interact with Rho proteins of plants (ROPs). Our in vivo and in vitro data demonstrate that RIP3 is a true ROP effector, interacting specifically with the active form of ROPs. We found that RIP3 ha...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.08.003
更新日期：2010-12-01 00:00:00
abstract：:Oocyte-thymocyte mouse cell hybrids were produced using polyethylene glycol (PEG) and examined at the ultrastructural level. Fusion was accomplished either before or after activation of metaphase II oocytes. In both experimental variants thymocyte nuclei undergo remodelling which comprises the following sequence of ev...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Triton-treated cells of the coenocytic green alga, Ernodesmis verticillata, manifest longitudinal contraction and centripetal closure of the cytoplasm in the presence of exogenous free calcium ions. Complete closure of the cytoplasm around the vacuolar remnants is obtained with 1 mM ATP present in the activation mediu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:This study reports the ultrastructural detection of fucosyl residues at the surface of axenically grown roots of Zea mays. The method used involved sequential binding of UeA lectin to the root and coupling of the bound UeA with fucosyl ferritin. Superficial dense ferritin labelling was found in the slime droplet enshe...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:During myocardial infarction, it is well known that the cytoplasm of cardiac myocytes becomes more acidic due to lactate accumulation. The resulting necrosis is believed to result, at least in part, from the leakage of lysosomal enzymes into the cytoplasm. In addition, it has previously been shown that cytoplasm acidi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:The isolation and characterization of highly purified and structurally well-preserved peroxisomes from the renal cortex of different mammalian species (beef, sheep, and cat) is reported. Renal cortex tissue was homogenized and a peroxisome-enriched light mitochondrial fraction was prepared by differential centrifugati...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
