Abstract:
:The subcellular distribution of a recently described neutral trimming mannosidase, the Man9-mannosidase, has been investigated by immunoelectron microscopy in pig hepatocytes. This enzyme processes asparagine-linked N-acetylglucosamine2 mannose9 oligosaccharides by specifically cleaving three of the four alpha 1,2-linked mannose residues and is distinct from the presumptive ER alpha-mannosidase and the two Golgi alpha-mannosidases. Specific polyclonal antibodies for Man9-mannosidase have been prepared, affinity-purified and characterized by immunoblotting. Immunolabeling using these antibodies was observed in the rough (rER) and smooth endoplasmic reticulum (sER) as well as transitional elements of the rough endoplasmic reticulum and smooth membrane profiles close to the Golgi apparatus while the cisternal stack of the Golgi apparatus was unlabeled. This indicates that Man9-mannosidase is an ER trimming mannosidase which acts on glycoproteins after glucosidase II trimming and before their transport to the Golgi apparatus. A comparison with glucosidase II by double immunolabeling showed a more restricted and local distribution of Man9-mannosidase since it was undetectable in the nuclear envelope and although present throughout the rER and sER, large portions of rER cisternae and many sER profiles were unlabeled. This local distribution of Man9-mannosidase might provide a morphological basis for its involvement in selective trimming reactions in the ER. The catabolic pathway for Man9-mannosidase, as for glucosidase II, may involve degradation in autophagic vacuoles.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Roth J,Brada D,Lackie PM,Schweden J,Bause Esubject
Has Abstractpub_date
1990-10-01 00:00:00pages
131-41issue
1eissn
0171-9335issn
1618-1298journal_volume
53pub_type
杂志文章abstract::Murine alpha1,2-mannosidase IB is a type II transmembrane protein localized to the Golgi apparatus where it is involved in the biogenesis of complex and hybrid N-glycans. This enzyme consists of a cytoplasmic tail, a transmembrane domain followed by a "stem" region and a large C-terminal catalytic domain. To analyze t...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00127
更新日期:2000-12-01 00:00:00
abstract::Appreciable amounts of glycosaminoglycans have been found by immunocytochemistry within mature elastin fibers of human dermis. On thin sections, elastin fibers showed antigenic sites for monoclonal antibodies recognizing the unsaturated units remaining after digestion of hyaluronic acid with Streptomyces hyaluronidase...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
abstract::Evolution of neoplastic cells has generally been regarded as a cumulative intrinsic process resulting in altered cell characteristics enabling enhanced growth properties, evasion of apoptotic signals, unlimited replicative potential and gain of properties enabling the ability to thrive in ectopic tissues and in some c...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1078/0171-9335-00346
更新日期:2003-11-01 00:00:00
abstract::Secretory granules of GH4C1 cells, a rat pituitary tumor cell line, are known to be induced by the treatment of estradiol (E2), insulin, and epidermal growth factor (EGF). We examined changes in the localization of cathepsins B, H, and L, lysosomal cysteine proteinases, in GH4C1 cells before and after hormonal treatme...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::A mitosis-specific centrosomal component was studied with a human autoantibody, SP-H, which immunostained mitotic poles and interphase nuclei, and a single polypeptide with an apparent molecular mass of 200 to 230 kDa in various lines of cultured cells. Early mitotic PtK1 cells treated with 10 micrograms/ml taxol cont...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::Among sarcomeric muscles the cardiac muscle cells are unique by, inter alia, a systemic and extended cell-cell contact structure, the intercalated disk (ID), comprising frequent and closely spaced arrays of plaque-coated cell-cell adhering junctions (AJs). As some of these junctions may look somewhat like desmosomes a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.11.003
更新日期:2006-02-01 00:00:00
abstract::The macronuclear DNA synthesis in well-fed carnivorous forms of Tetrahymena paravorax has been investigated with autoradiographic experiments. The macrostome cell type of this ciliate is induced by stomatin and fed on living prey (T. pyriformis). The DNA synthesis is re-initiated when the macrostomes become active pre...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::Dendritic cells (DC) are the most effective cells for antigen presentation in primary immune responses. Human cord blood CD34+ progenitors cultured in the presence of GM-CSF and TNF alpha generate a heterogeneous population of DC including Langerhans-like DC (LLDC) and monocytes. We describe here that IL-4 exerts diff...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80124-6
更新日期:1998-03-01 00:00:00
abstract::The taxonomic group of Orthobunyaviruses is gaining increased attention, as several emerging members are causing devastating illnesses among humans and livestock. These viruses are transmitted to mammals by arthropods (mostly mosquitoes) during the blood meal. The nature of their genomic RNA predisposes orthobunyaviru...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2015.06.001
更新日期:2015-07-01 00:00:00
abstract::Signal transduction elements, including protein kinase C, have been identified in mammalian spermatozoa. In order to evaluate the pattern of expression and the subcellular localization of nine different protein kinase C isoforms in the course of spermatogenesis, we utilized quantitative electron microscopy immunocytoc...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::In recent decades, research on peroxisome biogenesis has been particularly boosted since the role of these organelles in metabolism became unraveled. Indeed in plants, yeasts and fungi, peroxisomes play an important role in the adaptation of metabolism during developmental processes and/or altered environmental condit...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2010.04.001
更新日期:2010-09-01 00:00:00
abstract::ATP-dependent interactions between myosin and actin in the lower eukaryote, Physarum polycephalum, are inhibited by micromolar levels of Ca2+. This inhibition is mediated by the binding of Ca2+ to myosin, the phosphorylation of which is required if Ca2+ is to inhibit the activities of myosin (Kohama, K., Trends Pharma...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::Lipopolysaccharide (LPS) is known to mediate angiogenic effects in endothelial cells. The underlying mechanisms, however, remain largely unknown. In this study, we showed that LPS induced high motility group box protein 1 (HMGB1) secretion in human pulmonary microvascular endothelial cells (HPMECs). Knockdown and over...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.07.004
更新日期:2017-10-01 00:00:00
abstract::Monensin, a monovalent ionophore, caused swelling of mature cisternae of plant Golgi apparatus. The appearance of swollen cisternae was time-dependent and linear over a period of 1 h with an estimated maximum rate of production of one swollen cisterna every 3 to 4 min. Implicit in these observations was a need for the...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Cytochrome b5 has been visualized by an immunoferritin labeling in a Golgi preparation. When the Golgi apparatus was first disrupted before labeling, 60% of the profiles were clearly labeled with ferritin. This proportion of labeled vesicles was too high to be explained by contamination from other membranes. When the ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::A set of monoclonal antibodies directed against various gastric markers was produced in order to study the developmental expression of the gastric mucosa. A previously described monoclonal antibody, mab 146.14, labeled the proton pump (H+, K+) ATPase specifically located in gastric parietal cells. Mabs 15.1 and 121.17...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
abstract::In the sea urchin embryo there are at least two cell adhesion molecules related to mammalian cadherins, one of them, similar to E-cadherin, is expressed in embryos at very early developmental stages, the second appears at the blastula stage (G. Ghersi et al. Mech. Dev. 41, 47-55 (1993)). We show here that when sea urc...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract::Cells of Oocystis solitaria after treatment with inhibitors of a) microfibril orientation b) microfibril synthesis, and c) microfibril crystallization have been investigated with the freeze-fracturing technique. Changes in morphology of the E-face of the plasma membrane as a result of these treatments have been record...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-10-01 00:00:00
abstract::PinX1 was originally identified as a Pin2/TRF1-interacting protein that suppresses telomerase activity via its telomerase inhibitor domain (TID) and regulates the nucleolar localization of TRF1 in telomerase-positive cells. In addition to its telomeric localization, PinX1 can be found in the nucleoli of human cells. O...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2010.05.005
更新日期:2010-10-01 00:00:00
abstract::Malignant melanoma is an aggressive form of skin cancer with an increasing incidence worldwide. One way to address the pathology of the disease is through molecular research. In addition to the analysis of melanoma-relevant signaling pathways, the investigation of important transcription factors is a fundamental objec...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2013.10.003
更新日期:2014-01-01 00:00:00
abstract::This paper explores the mode of action of the tranquillizers chloral hydrate and diazepam during fertilization and mitosis of the first reproductive cell cycles in sea urchin eggs. Most striking effects of these drugs are the alteration of centrosomal material and the abnormal microtubule configurations during exposur...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80041-1
更新日期:1998-01-01 00:00:00
abstract::Mechanical tension governs fibroblast proliferation and survival and the homeostasis of the extracellular matrix to adapt its resistance to the mechanical requirements of the organs. To consolidate this view, we analysed the effect of tension release on the expression of molecules involved in the architecture and stab...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00181
更新日期:2001-07-01 00:00:00
abstract::The Semper cells in the ommatidia of the blowfly contain an impressive cytoskeleton consisting of hundreds of microtubules. The microtubules are intimately interwoven by an extensive system of the smooth endoplasmic reticulum. The cells were permeabilized by saponin treatment, subsequently incubated in a loading mediu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::The interaction between myelin basic protein (MBP) and G-actin was studied under nonpolymerizing conditions, i.e.,2mM HEPES, pH 7.5, 0.1 mM CaCl2 and 0.2 mM ATP. Fluorescence studies using pyrenyl-actin and the measurements of ATP hydrolysis rate show that MBP induces changes in the structure of the actin monomer simi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
abstract::A new perfusion medium for isolating cardiac myocytes from adult rats was developed, thereby yielding numerous viable cells with few morphological changes. The main factors in the isolation procedure are Ca2+ deficiency, collagenase, and mechanical dispersion. Their effects on the ultrastructure of cardiac myocytes we...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::Expression of the Cdx1 homeobox gene in epithelial intestinal cells promotes cellular growth and differentiation. Cdx1and the Pancreatitis Associated Protein I (PAP I) are concomitantly expressed in the epithelial cells of the lower part of the intestinal crypts. Because Cdx1 is a transcription factor and PAP I, in ot...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00148
更新日期:2001-02-01 00:00:00
abstract::The concept of pilot pseudopodia is reconsidered 30 years after its inauguration (Gerisch, G., Hülser, D., Malchow, D., Wick, U., 1975. Cell communication by periodic cyclic-AMP pulses. Phil. Trans. R. Soc. Lond. B 272, 181-192). The original hypothesis stated that protruding pseudopodia serve as dynamic sensory organ...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2006.05.002
更新日期:2006-09-01 00:00:00
abstract::MUC16/CA125 is over-expressed in human epithelial tumors including ovarian, breast and some other carcinomas. The purpose of this study is to investigate how cell surface MUC16 is functionally involved in tumor progression, with a special focus on the role of its cytoplasmic tail. Forced expression of C-terminal MUC16...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2013.10.005
更新日期:2013-08-01 00:00:00
abstract::When virgin temperature-sensitive mutant cdc28 cells of the yeast Saccharomyces cerevisiae were incubated at restrictive temperatures, they continued to grow, reaching a maximum of 3.3-fold the original size after 24 h. The protein and RNA levels increased during the first 24 h, then gradually decreased. The cells tha...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::Since various neuromuscular diseases are associated with abnormal glycosylation, it was of interest to determine whether this key post-translational modification is also altered in aged skeletal muscle. Lectins represent highly versatile carbohydrate-binding proteins that are routinely employed for the characterizatio...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2008.04.003
更新日期:2008-10-01 00:00:00