Abstract：
:ATP-dependent interactions between myosin and actin in the lower eukaryote, Physarum polycephalum, are inhibited by micromolar levels of Ca2+. This inhibition is mediated by the binding of Ca2+ to myosin, the phosphorylation of which is required if Ca2+ is to inhibit the activities of myosin (Kohama, K., Trends Pharmacol. Sci. 11, 433-435 (1990)). As the first step to examine whether Ca2+ also regulates phosphorylation in the actomyosin system, we purified myosin light chain kinase (MLCK) of 55 kDa almost to homogeneity. The MLCK activity was high whether or not Ca2+ was present. However, a Ca(2+)-dependent inhibitory factor (CIF) purified from Physarum (Okagaki et al., Biochem. Biophys. Res. Commun. 176, 564-570 (1991)) was shown to reduce the MLCK activity in a Ca(2+)-dependent manner. Using crude preparations, not only MLCK but also myosin heavy chain kinase and actin kinase were shown to be inhibited by Ca2+ half-maximally at micromolar levels. Since CIF is the only Ca(2+)-binding protein in the preparations, we propose that this inhibitory Ca(2+)-regulation of the kinases for actomyosin is mediated by CIF.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Okagaki T,Ishikawa R,Kohama Ksubject
Has Abstractpub_date
1991-10-01 00:00:00pages
113-22issue
1eissn
0171-9335issn
1618-1298journal_volume
56pub_type
杂志文章abstract：:When resting human blood platelets are stimulated with thrombin, 50 to 60% of the G-actin polymerizes to F-actin within 60 seconds. The increase in F-actin is correlated with a corresponding decrease in the complex of G-actin with T beta 4. Within 5 seconds after stimulation, nucleation sites for pyrene actin polymeri...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:To study molecules secreted from cultured plant cells that promote development, maize microspores were transferred into culture and the conditioned media were collected over time and analysed. Electrophoresis indicated that both non-glycosylated and glycosylated proteins including arabinogalactan proteins (AGPs) appea...
journal_title：European journal of cell biology
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abstract：:This electron microscopic study demonstrates that formation of a functional eukaryotic 40S initiation complex is accompanied by conformational changes which obscure the characteristic structural features of the 40S ribosomal subunits and of the initiation factor eIF-3, the only macromolecular components of the complex...
journal_title：European journal of cell biology
pub_type： 杂志文章
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更新日期：1983-11-01 00:00:00
abstract：:Many hormones/factors influence the total body growth and development during embryonic life, and very few studies have been carried out to ascertain their effects on the individual organ system. In this study, the effect of exogenous insulin-like growth factor-I (IGF-I) on embryonic kidneys was investigated, and corre...
journal_title：European journal of cell biology
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更新日期：1994-12-01 00:00:00
abstract：:The milk yeast Kluyveromyces lactis is an alternative model yeast to the well established Saccharomyces cerevisiae. The cell wall of these fungi consists of polysaccharides (i.e. long chains of β-1,3- and β-1,6-linked sugar chains and some chitin) and mannoproteins, both of which are continually adapted to environment...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
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abstract：:The evolutionary conserved transmembrane protein Crumbs (Crb) regulates morphogenesis of photoreceptor cells in the compound eye of Drosophila and prevents light-dependent retinal degeneration. Here we examine the role of Crb in the ocelli, the simple eyes of Drosophila. We show that Crb is expressed in ocellar photor...
journal_title：European journal of cell biology
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doi：10.1016/j.ejcb.2012.03.006
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journal_title：European journal of cell biology
pub_type： 杂志文章
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更新日期：1994-06-01 00:00:00
abstract：:Murine alpha1,2-mannosidase IB is a type II transmembrane protein localized to the Golgi apparatus where it is involved in the biogenesis of complex and hybrid N-glycans. This enzyme consists of a cytoplasmic tail, a transmembrane domain followed by a "stem" region and a large C-terminal catalytic domain. To analyze t...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00127
更新日期：2000-12-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2015.01.001
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2009.10.005
更新日期：2010-01-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2016.04.001
更新日期：2016-06-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:SGLT1, an isoform of Na+-dependent glucose cotransporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine and the kidney, where it plays a pivotal role in the absorption and reabsorption of sugars, respectively. To search the domain responsible for the apical localization of ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00204
更新日期：2001-12-01 00:00:00
abstract：:Three-dimensional collagen lattice cultures of fibroblasts mimic the in vivo situation better than monolayer cultures. Here, skin fibroblasts from scleroderma patients and healthy controls were cultivated in collagen lattices, and the effects of recombinant human gamma-interferon (IFN-gamma) on these cultures investig...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:High-speed centrifugation supernatants from slime mould plasmodia show considerable activities to inhibit the polymerization of actin as revealed by viscosity measurements. By following increasing inhibitory activities an actin modulating protein (AM-protein) has been isolated and purified which affects the polymer st...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:The mammalian skin has a photosensitive system comprised by several opsins, including rhodopsin (OPN2) and melanopsin (OPN4). Recently, our group showed that UVA (4.4 kJ/m2) leads to immediate pigment darkening (IPD) in murine normal and malignant melanocytes. We show the role of OPN2 and OPN4 as UVA sensors: UVA-indu...
journal_title：European journal of cell biology
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doi：10.1016/j.ejcb.2018.01.004
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-02-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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doi：
更新日期：1984-05-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1988-10-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-12-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1979-10-01 00:00:00
abstract：:The neonatal heart is a very useful tool for the study of biochemical pathways and properties of cardiomyocytes and as it has the potential to regenerate for a brief period of time from birth; it is also useful to study cardiac regeneration. However, as the heart matures, this proficiency for regeneration is reduced. ...
journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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doi：
更新日期：1985-05-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1984-01-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
