Abstract：
:PinX1 was originally identified as a Pin2/TRF1-interacting protein that suppresses telomerase activity via its telomerase inhibitor domain (TID) and regulates the nucleolar localization of TRF1 in telomerase-positive cells. In addition to its telomeric localization, PinX1 can be found in the nucleoli of human cells. Our recent studies have shown that PinX1 localizes to the chromosome periphery and kinetochores in mitosis. Depletion of PinX1 results in lagging chromosomes in mitosis and micronuclei in interphase. However, less is known about the post-translational modification of PinX1 in mitosis. Here, we show that Polo-like kinase 1 (Plk1) is a novel interacting protein of PinX1. Plk1 interacts with and phosphorylates PinX1 in vivo and in vitro. Overexpression of Plk1 promotes protein turnover of PinX1, a process that depends on ubiquitin-associated proteasomal degradation. Depletion of Plk1 using siRNA increases the stability of PinX1 at protein level in mitosis. Moreover, Plk1-mediated phosphorylation of PinX1 at five phosphorylation sites is essential for its Plk1-induced degradation. These findings suggest that Plk1 may negatively regulate the stability of PinX1 by mitotic phosphorylation.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Wang C,Yu J,Yuan K,Lan J,Jin C,Huang Hdoi
10.1016/j.ejcb.2010.05.005subject
Has Abstractpub_date
2010-10-01 00:00:00pages
748-56issue
10eissn
0171-9335issn
1618-1298pii
S0171-9335(10)00106-8journal_volume
89pub_type
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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