Abstract：
:Lamin proteins are components of metazoan cell nuclei. During evolution, two classes of lamin proteins evolved, A- and B-type lamins. B-type lamins are expressed in nearly all cell types and in all developmental stages and are thought to be indispensable for cellular survival. In contrast, A-type lamins have a more restricted expression pattern. They are expressed in differentiated cells and appear late in embryogenesis. In the earliest steps of mammalian development, A-type lamins are present in oocytes, pronuclei and during the first cleavage stages of the developing embryo. But latest after the 16-cell stage, A-type lamin proteins are not any longer detectable in embryonic cells. Amphibian oocytes and early embryos do not express lamin A. Moreover, extracts of Xenopus oocytes and eggs have the ability to selectively remove A-type lamins from somatic nuclei. This observation and the restricted expression pattern suggest that the presence of lamin A might interfere with developmental processes in the early phase of embryogenesis. To test this, we ectopically expressed lamin A during early embryonic development of Xenopus laevis by microinjection of synthetic mRNA. Here, we show that introducing mature lamin A does not interfere with normal development. However, expression of prelamin A or lamin A variants that cannot be fully processed cause severe disturbances and lead to apoptosis during gastrulation. The toxic effect is due to lack of the conversion of prenylated prelamin A to its mature form. Remarkably, even a cytoplasmic prelamin A variant that is excluded from the nucleus drives embryos into apoptosis.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Peter A,Stick Rdoi
10.1016/j.ejcb.2008.06.001subject
Has Abstractpub_date
2008-11-01 00:00:00pages
879-91issue
11eissn
0171-9335issn
1618-1298pii
S0171-9335(08)00093-9journal_volume
87pub_type
杂志文章abstract：:Sertoli cells in culture synthesize two membrane-associated proteoglycans (PGs) containing as glycosaminoglycan (GAG) moieties either chondroitin sulfate (CS) or CS and heparan sulfate (HS); the latter PG is, therefore, referred to as the mixed PG. To determine if these PGs are randomly distributed on the cell surface...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Monensin, a monovalent ionophore, caused swelling of mature cisternae of plant Golgi apparatus. The appearance of swollen cisternae was time-dependent and linear over a period of 1 h with an estimated maximum rate of production of one swollen cisterna every 3 to 4 min. Implicit in these observations was a need for the...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:It was previously shown that avidin, a glycoprotein secreted in vivo by chicken oviduct, is produced by cultured transformed or damaged chicken embryo fibroblasts [27]. This report demonstrates synthesis and secretion of large amounts of avidin by macrophages isolated from chicken yolk sac. Avidin was secreted to the ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Ovary gland cells of Aptenia cordifolia were exposed to 100 micrograms/ml cytochalasin B (cyt B) for 30 or 60 min during the phase of granulocrine polysaccharide secretion. The drug caused a congestion of Golgi vesicles around the dictyosomes, probably resulting from an inhibition of the vesicle migration towards the ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Exocytosis is mediated by high-affinity interactions between different SNARE proteins. The existence of several variants of each SNARE protein suggests that the specificity of fusion may be directed by unique combination of SNARE family members. We examined if two alternatively spliced variants of synaptosomal-associa...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00106
更新日期：2000-11-01 00:00:00
abstract：:Translationally controlled tumor protein (Tpt1/TCTP) is a multi-functional cytosolic protein whose cellular levels are finely tuned. TCTP regulates protein behavior by favoring stabilization of protein partners or on the contrary by promoting degradation of others. TCTP has been shown to be transcriptionally and trans...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2016.12.002
更新日期：2017-03-01 00:00:00
abstract：:The macronuclear DNA synthesis in well-fed carnivorous forms of Tetrahymena paravorax has been investigated with autoradiographic experiments. The macrostome cell type of this ciliate is induced by stomatin and fed on living prey (T. pyriformis). The DNA synthesis is re-initiated when the macrostomes become active pre...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-06-01 00:00:00
abstract：:Staphylococcus epidermidis is of major importance in infections associated with indwelling medical devices. The tight pathogenic association is essentially linked to the species ability to form adherent biofilms on artificial surfaces. Aiming at identifying novel targets for vaccination or therapy much effort has been...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2009.10.005
更新日期：2010-01-01 00:00:00
abstract：:A novel cytochemical method for the in situ, ultrastructural localization of phospholipids in biological tissues is reported. The method is based on the enzyme-gold approach (M. Bendayan: J. Histochem. Cytochem. 29, 531, 1981). Phospholipase A2 from bee venom was adsorbed on colloidal gold particles (PLA2-gold) and ap...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:In the sea urchin embryo there are at least two cell adhesion molecules related to mammalian cadherins, one of them, similar to E-cadherin, is expressed in embryos at very early developmental stages, the second appears at the blastula stage (G. Ghersi et al. Mech. Dev. 41, 47-55 (1993)). We show here that when sea urc...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：:The subcellular site of accumulation of non-esterified cholesterol and sphingomyelin in lipid-laden livers and spleens from rats given multiple intravenous injections with liposomes made up of these lipids were investigated by morphological and biochemical techniques. The subcellular fractionation of liver homogenates...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:Recently, we have described the developmental expression of the small heat shock proteins (sHsps) Hsp27/HspB1 and alphaB-crystallin/HspB5 in different tissues of pigs from almost full-term foetuses to three years old adults (P. Tallot, J. F. Grongnet, J. C. David, Biol. Neonate, 83, 281-288, 2003). The data described ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00337
更新日期：2003-10-01 00:00:00
abstract：:This electron microscopic study demonstrates that formation of a functional eukaryotic 40S initiation complex is accompanied by conformational changes which obscure the characteristic structural features of the 40S ribosomal subunits and of the initiation factor eIF-3, the only macromolecular components of the complex...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:The entry of sporozoites of Theileria parva into bovine lymphoid cells in vitro was studied with the electron microscope. Endocytosis is completed in less than 10 min. No local mobilization of actin or other cytoskeletal elements is detected in the cytoplasm of the cell being invaded and no engulfing pseudopods are fo...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:In giant neurons of whip spider legs several filament types are detectable: filaments of 5 to 6 nm thickness as dense masses within the soma of the neuron, an intermediate-sized filament type limited to the dendritic processes forming irregularly wound bundles and finally twisted double filaments in the soma as well a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:Glucocorticoid hormones regulate the transcription of nuclear genes by way of their cognate receptors. In addition, these hormones also modulate mitochondrial gene transcription by mechanisms which are as yet poorly understood. Using immunofluorescence labeling and confocal laser scanning microscopy we show that the g...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/S0171-9335(04)70033-3
更新日期：2000-05-01 00:00:00
abstract：:Polycationic derivatives of ferritin containing primary amino groups (CFah) or tertiary amino groups (CFdmp) were potent platelet agonists inducing shape change, aggregation and secretion, but also agglutination in the presence of EDTA. Pretreatment of platelets with neuraminidase, PGE1, indomethacin, or creatine kina...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:To get new insights into the function of the intermediate filament (IF) protein vimentin in cell physiology, we generated two mutant cDNAs, one with a point mutation in the consensus motif in coil1A (R113C) and one with the complete deletion of coil 2B of the rod domain. In keratins and glia filament protein (GFAP), a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2005.09.019
更新日期：2006-01-01 00:00:00
abstract：:Application of carbon tetrachloride produced a progressive proliferation of tight junctions in the rat liver. This system proved to be rapid and highly reproducable and affords the opportunity for tracing the fate of tight junctions in freeze-fracture replicas, facilitating investigations on their formation and functi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:Human enteric α-defensins (HD5 and HD6), major antimicrobial peptides produced by Paneth cells in the intestine, play important roles in intestinal innate immunity. Since their expression is decreased in Crohn's disease (CD), with decreased expression being more pronounced in the presence of NOD2 mutations, it would b...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2014.10.007
更新日期：2015-01-01 00:00:00
abstract：:The recently established in vitro assay of condensation-sorting of pancreatic enzymes to the zymogen granule membrane (ZGM) (Dartsch, H., R. Kleene, H. F. Kern: In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells. Eur. J. Cell Biol. 75, 211-222 (1998)) was used to study the invol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80009-0
更新日期：1999-02-01 00:00:00
abstract：:Dictyosomes, Golgi vesicles, and plasma membranes were investigated after freeze-fracture in cells from growing root tips of cress (Lepidium sativum L.), that are distinguishable by different cellulose content of the cell wall, into (i) meristematic cells during early formation of the cell plate, (ii) statocytes of th...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:By double indirect immunofluorescence and interference electron microscopy, we have observed the effect of microtubule disruption by antimitotic drugs and coldness treatment on the distribution of adhesion sites and of the three cytoskeletal proteins, vinculin, alpha-actinin, and actin in normal rat cells and in rat c...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:Endothelial cell-specific molecules are potential targets for new therapeutic strategies in the control of inflammatory reactions, immune responses and neoangiogenesis. We describe the production and characterization of MEC 14.7, a monoclonal antibody directed to murine endothelial cells recognizing a glycosylated pro...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:The taxonomic group of Orthobunyaviruses is gaining increased attention, as several emerging members are causing devastating illnesses among humans and livestock. These viruses are transmitted to mammals by arthropods (mostly mosquitoes) during the blood meal. The nature of their genomic RNA predisposes orthobunyaviru...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2015.06.001
更新日期：2015-07-01 00:00:00
abstract：:Specific rabbit antisera were prepared against purified aggregation factor and its membrane-associated receptor, baseplate, derived from the marine sponge. Microciona prolifera. They were utilized in conjunction with fluorescent-labeled goat anti-rabbit IgG in an assay to demonstrate the surface localizations of both ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:Kynurenic acid (KYNA), a tryptophan metabolite in the kynurenine pathway, is protective against various insults. However, the molecular mechanism of this protective effect has not been identified. In this study, we examined the protective effects of KYNA against 1-methyl-4-phenylpyridinium (MPP(+)), the best-character...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2008.03.003
更新日期：2008-06-01 00:00:00
abstract：:LIM kinase (LIMK) plays a critical role in stimulus-induced remodeling of the actin cytoskeleton by linking signals from the Rho family GTPases to changes in cofilin activity. Recent studies have shown an important role for LIMK1 signaling in tumor cell invasion through regulating actin dynamics. In this study, we inv...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00382
更新日期：2004-08-01 00:00:00
abstract：:In Drosophila embryos, the dorsal gene, a member of the rel family of transcription factors, is a maternal gene required for dorsal/ventral axis specification and mesoderm and neural migration and differentiation. In this report, the presence and distribution of Xrel-1 protein, a Xenopus homologue of the rel family of...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Dipyridamole (DPD), a widely used anti-thrombotic agent, inhibits in vitro directional migration of 10T1/2 mouse embryo cells in a dose dependent way. The inhibition is detectable at DPD concentrations comparable to those in human plasma after oral intake of the drug. Using a model for quantitative shape change analys...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
