Localization of glucocorticoid hormone receptors in mitochondria of human cells.

Abstract:

:Glucocorticoid hormones regulate the transcription of nuclear genes by way of their cognate receptors. In addition, these hormones also modulate mitochondrial gene transcription by mechanisms which are as yet poorly understood. Using immunofluorescence labeling and confocal laser scanning microscopy we show that the glucocorticoid receptor of HeLa and Hep-2 cells is specifically enriched at the sites of the mitochondria which were visualized by labeling with the vital dye CMX and antibodies against cytochrome oxidase subunit I. Immunogold electron microscopy demonstrated that the receptor was located within the inner space of the mitochondria. Immunoblotting experiments also revealed the presence of glucocorticoid receptor in mitochondria isolated from HeLa and Hep-2 cells. Finally, living HeLa cells expressing green fluorescent-glucocorticoid receptor fusion protein revealed a distinct mitochondrial GFP fluorescence. Our results support the concept of a receptor-mediated direct action of steroid hormones on mitochondrial gene transcription.

journal_name

Eur J Cell Biol

authors

Scheller K,Sekeris CE,Krohne G,Hock R,Hansen IA,Scheer U

doi

10.1078/S0171-9335(04)70033-3

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

299-307

issue

5

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(04)70033-3

journal_volume

79

pub_type

杂志文章
  • Expression of vimentin and nuclear lamins during the in vitro differentiation of human promyelocytic leukemia cells HL-60.

    abstract::We have reported previously that the human promyelocytic leukemia cell line HL-60, in its undifferentiated state, is devoid of cytoplasmic intermediate filament proteins and nuclear lamins A and C, but does express lamin B. Using immunofluorescence and immunoblotting techniques, we have further investigated the expres...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Paulin-Levasseur M,Giese G,Scherbarth A,Traub P

    更新日期:1989-12-01 00:00:00

  • LPS binding to HMGB1 promotes angiogenic behavior of endothelial cells through inhibition of p120 and CD31 via ERK/P38/Src signaling.

    abstract::Lipopolysaccharide (LPS) is known to mediate angiogenic effects in endothelial cells. The underlying mechanisms, however, remain largely unknown. In this study, we showed that LPS induced high motility group box protein 1 (HMGB1) secretion in human pulmonary microvascular endothelial cells (HPMECs). Knockdown and over...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2017.07.004

    authors: Liu Z,Wang J,Xing W,Peng Y,Quan J,Fan X

    更新日期:2017-10-01 00:00:00

  • Integrins mediate the effects of quinolones and magnesium deficiency on cultured rat chondrocytes.

    abstract::Chondrocyte-matrix interaction is mediated by a series of adhesion molecules. Both alpha and beta integrin subunits are involved and govern crucial functions of cell adhesion and signal transduction. These molecules modulate proliferation and differentiation, thus establishing cartilage integrity. We studied the influ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/s0171-9335(99)80081-8

    authors: Egerbacher M,Wolfesberger B,Walter I,Seirberl G

    更新日期:1999-06-01 00:00:00

  • Ultrastructural detection of fucosyl residues at the surface of axenically grown maize roots/sequential use of UeA lectin and fucosyl ferritin as the specific glycosylated marker.

    abstract::This study reports the ultrastructural detection of fucosyl residues at the surface of axenically grown roots of Zea mays. The method used involved sequential binding of UeA lectin to the root and coupling of the bound UeA with fucosyl ferritin. Superficial dense ferritin labelling was found in the slime droplet enshe...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Rougier M

    更新日期:1984-05-01 00:00:00

  • Remodelling of thymocyte nuclei in activated mouse oocytes: an ultrastructural study.

    abstract::Oocyte-thymocyte mouse cell hybrids were produced using polyethylene glycol (PEG) and examined at the ultrastructural level. Fusion was accomplished either before or after activation of metaphase II oocytes. In both experimental variants thymocyte nuclei undergo remodelling which comprises the following sequence of ev...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Szöllösi D,Czołowska R,Sołtynska MS,Tarkowski AK

    更新日期:1986-10-01 00:00:00

  • Regulation of the maize (Zea mays L.) embryo proteome by RTCS which controls seminal root initiation.

    abstract::Seminal roots are initiated at the scutellar node during maize (Zea mays L.) embryo development. The maize mutant rtcs (rootless concerning crown and seminal roots) does not initiate seminal roots while its wild-type siblings form on average 2.9 seminal roots per seedling. In this study, proteome profiles of 25-day-ol...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2009.11.014

    authors: Muthreich N,Schützenmeister A,Schütz W,Madlung J,Krug K,Nordheim A,Piepho HP,Hochholdinger F

    更新日期:2010-02-01 00:00:00

  • A radioimmunoassay to monitor synaptic activity in hippocampal neurons in vitro.

    abstract::Exocytosis of synaptic vesicles (SV) results in the surface exposure of lumenal epitopes of SV proteins. We have recently described the use of antibodies directed against the lumenal N-terminus of synaptotagmin I (Sytlum-Abs) to morphologically monitor exo-endocytic recycling of SVs. We report here that a radioimmunoa...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Mundigl O,Verderio C,Krazewski K,De Camilli P,Matteoli M

    更新日期:1995-03-01 00:00:00

  • Mutations in vimentin disrupt the cytoskeleton in fibroblasts and delay execution of apoptosis.

    abstract::To get new insights into the function of the intermediate filament (IF) protein vimentin in cell physiology, we generated two mutant cDNAs, one with a point mutation in the consensus motif in coil1A (R113C) and one with the complete deletion of coil 2B of the rod domain. In keratins and glia filament protein (GFAP), a...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2005.09.019

    authors: Schietke R,Bröhl D,Wedig T,Mücke N,Herrmann H,Magin TM

    更新日期:2006-01-01 00:00:00

  • Terrestrial vertebrates have two keratin gene clusters; striking differences in teleost fish.

    abstract::Keratins I and II form the largest subgroups of mammalian intermediate filament (IF) proteins and account as obligatory heteropolymers for the keratin filaments of epithelia. All human type I genes except for the K18 gene are clustered on chromosome 17q21, while all type II genes form a cluster on chromosome 12q13, th...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2005.01.007

    authors: Zimek A,Weber K

    更新日期:2005-06-01 00:00:00

  • Phosphorylation of p97(VCP) and p47 in vitro by p34cdc2 kinase.

    abstract::The hexameric ATPase p97/yeast Cdc48p has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. p97/Cdc48p contains two conserved consensus p34cdc2 kinase phosphorylation sites with...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/S0171-9335(99)80055-7

    authors: Mayr PS,Allan VJ,Woodman PG

    更新日期:1999-04-01 00:00:00

  • Uridine, but not cytidine can sustain growth of Ehrlich ascites tumor cells in glucose-deprived medium with altered proliferation kinetics.

    abstract::Cell cycle kinetics and energy metabolism of Ehrlich ascites tumor cells grown in glucose-deprived medium supplemented with uridine, were investigated in order to extend our knowledge about the significance of the metabolic conversion of glucose for cell cycle progression of these cells. Viability (dye exclusion test)...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Linker W,Löffler M,Schneider F

    更新日期:1985-03-01 00:00:00

  • Is the sensitivity of cells for FGF-1 and FGF-2 regulated by cell surface heparan sulfate proteoglycans?

    abstract::We have examined the importance of cell surface heparan sulfate proteoglycans (HSPG) in fibroblast growth factor (FGF) signaling. 3T3 cells grown under conventional conditions were much more sensitive to FGF-2 compared to FGF-1. However, cells were equally sensitive to FGF-1 and FGF-2 using conditions which reduced th...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Zhou FY,Owens RT,Hermonen J,Jalkanen M,Höök M

    更新日期:1997-06-01 00:00:00

  • The COP9 signalosome and its role in plant development.

    abstract::The COP9 signalosome (CSN) is an evolutionarily conserved multiprotein complex with a role in the regulation of cullin-RING type E3 ubiquitin ligases (CRLs). CSN exerts its function on E3 ligases by deconjugating the ubiquitin-related protein NEDD8 from the CRL cullin subunit. Thereby, CSN has an impact on multiple CR...

    journal_title:European journal of cell biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ejcb.2009.11.021

    authors: Schwechheimer C,Isono E

    更新日期:2010-02-01 00:00:00

  • Nipah virus fusion protein: Importance of the cytoplasmic tail for endosomal trafficking and bioactivity.

    abstract::Nipah virus (NiV) is a highly pathogenic paramyxovirus which encodes two surface glycoproteins: the receptor-binding protein G and the fusion protein F. As for all paramyxoviruses, proteolytic activation of the NiV-F protein is an indispensable prerequisite for viral infectivity. Interestingly, proteolytic activation ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2015.05.005

    authors: Weis M,Maisner A

    更新日期:2015-07-01 00:00:00

  • Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors.

    abstract::Endothelial cell-specific molecules are potential targets for new therapeutic strategies in the control of inflammatory reactions, immune responses and neoangiogenesis. We describe the production and characterization of MEC 14.7, a monoclonal antibody directed to murine endothelial cells recognizing a glycosylated pro...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Garlanda C,Berthier R,Garin J,Stoppacciaro A,Ruco L,Vittet D,Gulino D,Matteucci C,Mantovani A,Vecchi A,Dejana E

    更新日期:1997-08-01 00:00:00

  • Isolation and biochemical characterization of the costa of Tritrichomonas foetus.

    abstract::The costae are cytoskeletal structures found in Trichomonadidae. Both the structural organization and composition of this organelle are still unknown. In the present work we have introduced a new methodology for the costa isolation. Using sucrose density-gradient centrifugation an enriched costa fraction was obtained....

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Monteiro-Leal LH,da Cunha-e-Silva NL,Benchimol M,de Souza W

    更新日期:1993-04-01 00:00:00

  • Microtubule polyglutamylation in Drosophila melanogaster brain and testis.

    abstract::The presence of glutamylated tubulin, a widespread posttranslational modification of alpha- and beta-tubulin, has been investigated in Drosophila melanogaster using the specific monoclonal antibody GT335. We show here that this modification is strongly detected in brain and testis whereas other tissues analyzed did no...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/S0171-9335(99)80053-3

    authors: Bobinnec Y,Marcaillou C,Debec A

    更新日期:1999-09-01 00:00:00

  • A simple strategy for generation of gene knockdown constructs with convergent H1 and U6 promoters.

    abstract::RNA interference (RNAi) is a powerful tool for functional genetic studies in model organisms and mammalian cells. To facilitate rapid construction of gene knockdown constructs and RNAi libraries for known genes of mammalian cells, a new and simple strategy to produce small interfering RNA (siRNA) expression vectors wi...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2005.10.009

    authors: Jian R,Peng T,Deng S,Jiang J,Hu F,An J,Cheng X

    更新日期:2006-05-01 00:00:00

  • Heteromeric connexons formed by the lens connexins, connexin43 and connexin56.

    abstract::In the eye lens, three connexins have been detected in epithelial cells and bow region/differentiating fiber cells, suggesting the possible formation of heteromeric gap junction channels. To study possible interactions between Cx56 and Cx43, we stably transfected a normal rat kidney cell line (NRK) that expresses Cx43...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1078/0171-9335-00132

    authors: Berthoud VM,Montegna EA,Atal N,Aithal NH,Brink PR,Beyer EC

    更新日期:2001-01-01 00:00:00

  • Cytochemical localization of lipases during spore germination from Bryum capillare.

    abstract::In the oil containing spores of Bryum capillare, lipid breakdown occurs within three days after sowing. The periods of activity of acid, neutral and alkaline lipases are coincident. They start at the first day after sowing the spores. After 3 days of germination, when storage fats forming large globules are mobilised ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Carrapiço F,Salomé M,Pais S

    更新日期:1983-03-01 00:00:00

  • Interphase cells of the centric diatom, Thalassiosira fluviatilis, lack detyrosinated, nontyrosinatable and acetylated tubulin.

    abstract::Within eukaryotic cells tubulin generally exists in protein families composed of closely related isoforms generated either by differential gene transcription or by posttranscriptional and posttranslational mechanisms. In this study, immunological approaches were used to examine the contribution of posttranslational mo...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Machell NH,Fritz L,MacRae TH

    更新日期:1995-05-01 00:00:00

  • Characterization of the deoxynucleoside-dependent reversal of hypoxia-induced inhibition of cell cycle progression in Ehrlich ascites tumor cells.

    abstract::Studies on Ehrlich ascites tumor cells enriched in different cell cycle compartments by centrifugal elutriation have shown that after exclusion of oxygen, G1- and early S-phase cells are restricted from further cell cycle progression. On addition of a balanced mixture of deoxypyrimidine and deoxypurine nucleosides to ...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Löffler M

    更新日期:1985-11-01 00:00:00

  • Isolation and identification of actin from the phytopathogenic filamentous fungus uromyces appendiculatus.

    abstract::Crude protein extracts of Uromyces appendiculatus contain a polypeptide that resembles actin in several ways. This protein eluates from DEAE-cellulose with concentrations of KCl known to release actin of other species from the cation. The polypeptide is recognized by polyclonal antibodies directed to sodium dodecyl su...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Tiburzy R,Hoch HC,Staples RC

    更新日期:1990-12-01 00:00:00

  • Cysteine proteinases in GH4C1 cells, a rat pituitary tumor cell line, are secreted by the constitutive and regulated secretory pathways.

    abstract::Secretory granules of GH4C1 cells, a rat pituitary tumor cell line, are known to be induced by the treatment of estradiol (E2), insulin, and epidermal growth factor (EGF). We examined changes in the localization of cathepsins B, H, and L, lysosomal cysteine proteinases, in GH4C1 cells before and after hormonal treatme...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Waguri S,Sato N,Watanabe T,Ishidoh K,Kominami E,Sato K,Uchiyama Y

    更新日期:1995-08-01 00:00:00

  • Cationized ferritin as a platelet-stimulating surface probe. Binding to platelets and effects on platelet function.

    abstract::Polycationic derivatives of ferritin containing primary amino groups (CFah) or tertiary amino groups (CFdmp) were potent platelet agonists inducing shape change, aggregation and secretion, but also agglutination in the presence of EDTA. Pretreatment of platelets with neuraminidase, PGE1, indomethacin, or creatine kina...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Patscheke H,Dierichs R

    更新日期:1986-03-01 00:00:00

  • The widespread human desmocollin Dsc2 and tissue-specific patterns of synthesis of various desmocollin subtypes.

    abstract::By comparison of the cDNA-derived amino acid sequences and the cell type-specific patterns of synthesis we have identified desmocollin Dsc2 as the most widespread, perhaps ubiquitous desmocollin subtype. Using Northern blot analyses and ribonuclease protection assays we have found an approximately 5.6 kb mRNA encoding...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Nuber UA,Schäfer S,Schmidt A,Koch PJ,Franke WW

    更新日期:1995-01-01 00:00:00

  • Effects of daunomycin on the microtubular network: a cytochemical study on a human melanoma cell line.

    abstract::The interaction of daunomycin (DAU), an anthracyclinic antibiotic employed as antitumoral agent, with microtubules, has been investigated by cytochemical and morphological methods on a human melanoma cell line (H14). Results obtained indicated that DAU was able to modulate the microtubule reassembly in cells treated w...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Molinari A,Calcabrini A,Crateri P,Arancia G

    更新日期:1991-04-01 00:00:00

  • Isolation of zymogen granules from rat pancreas and characterization of their membrane proteins.

    abstract::A zymogen granule fraction has been isolated from rat pancreas, and its purity has been assessed by biochemical and morphological criteria. Specific activities of two marker enzymes, amylase and chymotrypsin, are increased by 4.6 and 5.4-fold, respectively, as compared to the homogenate. The purified fraction is devoi...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Pâquet MR,St-Jean P,Roberge M,Beaudoin AR

    更新日期:1982-08-01 00:00:00

  • Acetylation of translationally controlled tumor protein promotes its degradation through chaperone-mediated autophagy.

    abstract::Translationally controlled tumor protein (Tpt1/TCTP) is a multi-functional cytosolic protein whose cellular levels are finely tuned. TCTP regulates protein behavior by favoring stabilization of protein partners or on the contrary by promoting degradation of others. TCTP has been shown to be transcriptionally and trans...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:10.1016/j.ejcb.2016.12.002

    authors: Bonhoure A,Vallentin A,Martin M,Senff-Ribeiro A,Amson R,Telerman A,Vidal M

    更新日期:2017-03-01 00:00:00

  • Evidence for the presence of proteolytic activity in peroxisomes.

    abstract::The presence of endo- and exoproteolytic activity in peroxisomes was detected in cell organelles purified from pea leaves. By PAGE using different exopeptidase substrates (L-aa-beta NA), one leucine aminopeptidase (AP) was found in peroxisomes. The peroxisomal AP was characterized as a serine protease and had a maxima...

    journal_title:European journal of cell biology

    pub_type: 杂志文章

    doi:

    authors: Corpas FJ,Palma JM,del Río LA

    更新日期:1993-06-01 00:00:00