Abstract:
:The entry of sporozoites of Theileria parva into bovine lymphoid cells in vitro was studied with the electron microscope. Endocytosis is completed in less than 10 min. No local mobilization of actin or other cytoskeletal elements is detected in the cytoplasm of the cell being invaded and no engulfing pseudopods are formed. At the site of initial contact, the membranes of parasite and host cell come into very close apposition. As the zippering up of the membranes spreads laterally, the sporozoite sinks into a progressively deepening recess in the surface of the host cell until the rim of the invagination closes and fuses over the parasite. The observation that sporozoites are interiorized at 1-2 degrees C as well as at 37 degrees C suggests that endocytosis depends mainly upon a ligand-receptor interaction of the parasite and host cell membranes and requires little energy. Sporozoites may enter in any orientation, unlike other sporozoan parasites in which the membrane overlying an apical complex is invariably the site of attachment. 24 h after entry, the sporozoite is located in the Golgi region and the investing host cell membrane acquired during endocytosis has disappeared. The Golgi complex has been activated to form small lysosomes which gather around the parasite but are ineffective for lack of a membrane which they can fuse. It is suggested that removal of the investing host-cell membrane permits the parasite to evade destruction by the phagolysosomal system of the host cell. Persistence of micronemes after entry of the sporozoite and their subsequent disappearances invites the speculation that these parasite organelles may play a role in dispersal of the invaginated host cell membrane.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Fawcett DW,Doxsey S,Stagg DA,Young ASsubject
Has Abstractpub_date
1982-04-01 00:00:00pages
10-21issue
1eissn
0171-9335issn
1618-1298journal_volume
27pub_type
杂志文章abstract::Tight junctions (TJ) of the fascia occludens type can be rapidly assembled in the human colon adenocarcinoma cell line HT 29 under the influence of trypsin or ammonium sulfate. We have studied the influence of the metabolic inhibitors, dinitrophenol (DNP) and deoxyglucose (DG), on the induced formation of TJ in this c...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::In recent years, several antagonists of alpha(v)beta3 have been used to develop therapeutic approaches to the treatment of melanoma neoplasia. We studied the effects of anti-alpha(v)-integrin-blocking antibodies on attached M21 melanoma cells, the cellular distribution of alpha(v)-integrin and the molecular organizati...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00067
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journal_title:European journal of cell biology
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doi:10.1078/0171-9335-00298
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1988-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1988-04-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-07-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2008.03.003
更新日期:2008-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2012.03.006
更新日期:2012-09-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00337
更新日期:2003-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Murine alpha1,2-mannosidase IB is a type II transmembrane protein localized to the Golgi apparatus where it is involved in the biogenesis of complex and hybrid N-glycans. This enzyme consists of a cytoplasmic tail, a transmembrane domain followed by a "stem" region and a large C-terminal catalytic domain. To analyze t...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00127
更新日期:2000-12-01 00:00:00
abstract::Epidermal Langerhans cells (LC) play a key role in initiation and regulation of immune responses. Whereas LC migration out of the epidermis upon environmental assault is extensively studied, the mechanisms involved in the (re)population of the epidermis with LC are poorly understood. Here, we investigated the immigrat...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2012.06.004
更新日期:2012-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.02.002
更新日期:2005-07-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::The rabies virus (RV) phosphoprotein P is a multifunctional protein involved in viral RNA synthesis and in counteracting host innate immune responses. We have previously shown that RV P gene expression levels can be regulated by using picornavirus internal ribosome entry site (IRES) elements. Here we exploited a parti...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2011.01.009
更新日期:2012-01-01 00:00:00
abstract::In flowering plants, gametes are formed in specialized haploid structures, termed gametophytes. The female gametophyte is a few-celled structure that integrates such diverse functions as pollen tube attraction, sperm cell release, gamete fusion and seed initiation. These processes are realized by distinct cell types, ...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2009.11.005
更新日期:2010-02-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2013.07.001
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/s0171-9335(99)80034-x
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(99)80055-7
更新日期:1999-04-01 00:00:00
abstract::Fas ligand can both be used by the immune system to initiate cell death, and be used by non-lymphoid cells to evade death. Recent work has shown that Fas ligand is differentially sorted in different cell types. Here we present the viewpoint that the differential sorting plays an important part in determining the role ...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1078/0171-9335-00077
更新日期:2000-08-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00434
更新日期:2004-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::Temperatures around 20 degrees C are known to block degradation of endocytosed material by preventing its transport to lysosomes, accordingly reduced temperature has been widely used to define endosomes. Newer studies have revealed that the low temperature block is proximal to perinuclear late endosomes, but it is not...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/s0171-9335(98)80067-8
更新日期:1998-04-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/s0171-9335(99)80081-8
更新日期:1999-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.04.004
更新日期:2017-09-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2005.10.006
更新日期:2006-02-01 00:00:00