Abstract：
:The evolutionary conserved transmembrane protein Crumbs (Crb) regulates morphogenesis of photoreceptor cells in the compound eye of Drosophila and prevents light-dependent retinal degeneration. Here we examine the role of Crb in the ocelli, the simple eyes of Drosophila. We show that Crb is expressed in ocellar photoreceptor cells, where it defines a stalk membrane apical to the adherens junctions, similar as in photoreceptor cells of the compound eyes. Loss of function of crb disrupts polarity of ocellar photoreceptor cells, and results in mislocalisation of adherens junction proteins. This phenotype is more severe than that observed in mutant photoreceptor cells of the compound eye, and resembles more that of embryonic epithelia lacking crb. Similar as in compound eyes, crb protects ocellar photoreceptors from light induced degeneration, a function that depends on the extracellular portion of the Crb protein. Our data demonstrate that the function of crb in photoreceptor development and homeostasis is conserved in compound eyes and ocelli and underscores the evolutionarily relationship between these visual sense organs of Drosophila. The data will be discussed with respect to the difference in apico-basal organisation of these two cell types.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Mishra M,Rentsch M,Knust Edoi
10.1016/j.ejcb.2012.03.006subject
Has Abstractpub_date
2012-09-01 00:00:00pages
706-16issue
9eissn
0171-9335issn
1618-1298pii
S0171-9335(12)00059-3journal_volume
91pub_type
杂志文章abstract：:Taking advantage of monoclonal antibodies raised against chick hypertrophic chondrocytes (BD5, LA5, AD2) and osteoblasts (SB1, SB2, SB3, SB5), we have investigated expression of differentiation stage-specific antigens by further differentiating hypertrophic chondrocytes. Expression of all antigens was developmentally ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-06-01 00:00:00
abstract：:Tetrahymena pyriformis GL is a suitable model to study the influence on phagocytotic activity of two membrane-stabilizing benzimidazole nitrogen mustard derivative, ZIMET 3164 and ZIMET 3393 (cytostasan). Treatment by both compounds causes a gradual inhibition of food vacuole formation dependent on the concentrations ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:It was previously shown that avidin, a glycoprotein secreted in vivo by chicken oviduct, is produced by cultured transformed or damaged chicken embryo fibroblasts [27]. This report demonstrates synthesis and secretion of large amounts of avidin by macrophages isolated from chicken yolk sac. Avidin was secreted to the ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:In the presence of various commonly used buffers, phosphate-buffered saline (PBS), tris-buffered saline (TBS), Na-cacodylate, bovine serum albumin and a wide range of cytochemically active proteins (monoclonal and polyclonal IgG, concanavalin A, Ricinus communis lectin I, Helix pomatia lectin, protein A) were complexe...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:Mouse plasma cells have been exposed to a wide range of soluble and adsorptive macromolecular tracers for 10 min to 4 h to explore the possibility of membrane recycling related to the high secretory rate of these nonregulated secretory cells. Electron microscopic examination showed in all cases labeling of multivesicu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-12-01 00:00:00
abstract：:Initiation of eukaryotic DNA replication is achieved by the sequential binding of different proteins to origins of DNA replication. Using EGFP-tagged initiator proteins and immunofluorescence techniques we found that most of the ORC and the MCM subunits are localised at centrosomes and are colocalised with the polo-li...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2006.09.002
更新日期：2007-01-01 00:00:00
abstract：:Pretreatment of Acanthamoeba castellanii (Neff strain) with sublytic concentrations of surface-active alkyltrimethylammonium salts (C12, C14, C16) enhanced ConA-mediated agglutination of the amoebae. Treatment with the surfactants alone did not affect the "spontaneous" agglutination of the amoebae. Electron microscopi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:Actin, myosin, and the actin-associated proteins tropomyosin, alpha-actinin, vinculin, and villin were localized in acinar cells of rat and bovine pancreas, parotid, and prostate glands by means of immunofluorescent staining of both frozen tissue sections and semithin sections of quick-frozen, freeze-dried, and plasti...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Hepatocytoma (HPCT) hybrid cells were obtained by fusion of cultured rat hepatocytes with Fao Reuber hepatoma cells H35 by polyethylene glycol treatment. Surviving cells were cloned in HAT (hypoxanthine-aminopterine-thymidine)/ouabain medium and propagated in cell lines over 80 passages. Morphological criteria were ch...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:When virgin temperature-sensitive mutant cdc28 cells of the yeast Saccharomyces cerevisiae were incubated at restrictive temperatures, they continued to grow, reaching a maximum of 3.3-fold the original size after 24 h. The protein and RNA levels increased during the first 24 h, then gradually decreased. The cells tha...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Crude protein extracts of Uromyces appendiculatus contain a polypeptide that resembles actin in several ways. This protein eluates from DEAE-cellulose with concentrations of KCl known to release actin of other species from the cation. The polypeptide is recognized by polyclonal antibodies directed to sodium dodecyl su...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Using [3H]myo-inositol incorporation, changes in phosphoinositol (PI) metabolism at different cell cycle stages in the myxomycete Physarum polycephalum were examined by column chromatography. Two base levels for the inositol trisphosphate (IP3) fraction were determined: a low one for S-phase and a higher one for G2 ph...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:Murine alpha1,2-mannosidase IB is a type II transmembrane protein localized to the Golgi apparatus where it is involved in the biogenesis of complex and hybrid N-glycans. This enzyme consists of a cytoplasmic tail, a transmembrane domain followed by a "stem" region and a large C-terminal catalytic domain. To analyze t...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00127
更新日期：2000-12-01 00:00:00
abstract：:The transport of the chitin synthase III, Chs3p, to the plasma membrane is temporally and spatially regulated. Chs3p is delivered to the plasma membrane at the beginning of the cell cycle, forming chitin rings, and at the end of the cell cycle, forming the primary septum. During the rest of the cell cycle, it is maint...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2005.10.006
更新日期：2006-02-01 00:00:00
abstract：:Formation of the complete spindles during the budding process of Saccharomyces uvarum was investigated by fluorescence microscopy of protoplasted cells. Protoplasts were treated with anti-tubulin antibodies and DAPI, a fluorescent dye staining DNA. Thus, both chromatin and spindles could be visualized. Duplication as ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:We have investigated the effect of stepwise truncating the carboxyterminal domain ("tail") of the intermediate filament (IF) protein vimentin of the clawed toad, Xenopus laevis, on filament assembly in vitro and, using cell transfection, in vivo and also on the cellular topology of the structures formed. All truncatio...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:In the present study, a systematic examination of the relative expression pattern of the nuclear retinoid receptors (RAR and RXR) in various liver cells was performed. Our data demonstrate that RXRalpha is the dominant receptor in all liver cells, and that RARbeta is also expressed at a high level in most cells. More ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80078-2
更新日期：1998-10-01 00:00:00
abstract：:The differences in the content of trace elements (Fe, Cu, Zn, Br, Rb and Sr) in phasic and tonic scallop adductor muscles have been revealed by using X-ray fluorescence analysis. The phasic (yellow) muscles have been found to be richer in Fe and Cu while more deficient in Zn and Br as compared to the tonic (white) mus...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:Studies on Ehrlich ascites tumor cells enriched in different cell cycle compartments by centrifugal elutriation have shown that after exclusion of oxygen, G1- and early S-phase cells are restricted from further cell cycle progression. On addition of a balanced mixture of deoxypyrimidine and deoxypurine nucleosides to ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：:This study reports the ultrastructural detection of fucosyl residues at the surface of axenically grown roots of Zea mays. The method used involved sequential binding of UeA lectin to the root and coupling of the bound UeA with fucosyl ferritin. Superficial dense ferritin labelling was found in the slime droplet enshe...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:The distribution of microtubules and centrosomes was studied during sheep fertilization by electron and immunofluorescence microscopy. Tubulin and centrosomal material was identified with monoclonal anti-alpha-tubulin and MPM-2 antibodies, respectively. In ovulated eggs, microtubules were exclusively found in the meio...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:Despite its frequent inactivation in human breast cancers, the role of p21(Cip1) (p21) in morphological plasticity of normal mammary epithelial cells is still poorly understood. To address this question, we have investigated the consequences of p21 silencing in two-dimensional (2D) morphogenesis of untransformed human...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2011.03.002
更新日期：2011-08-01 00:00:00
abstract：:Phagosomes with pathogenic mycobacteria, or with hydrophobic polystyrene beads of 1 micron in size, do not mature, but remain fusogenic towards early endosomes and do not fuse with lysosomes (de Chastellier et al. Eur. J. Cell Biol. 68, 167-182 (1995)). Both types of phagocytic particles display a close apposition to ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:CD14-positive monocytes obtained from human peripheral blood were cultured with GM-CSF and IL-4. During the early culture phase immature dendritic cells (DCs) developed which not only expressed CD1a, HLA-DR and CD86, but also expressed the endothelial cell markers von Willebrand factor (vWF), VE-cadherin and VEGF rece...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00136
更新日期：2001-01-01 00:00:00
abstract：:Monensin, a monovalent ionophore, caused swelling of mature cisternae of plant Golgi apparatus. The appearance of swollen cisternae was time-dependent and linear over a period of 1 h with an estimated maximum rate of production of one swollen cisterna every 3 to 4 min. Implicit in these observations was a need for the...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:Exocytosis of synaptic vesicles (SV) results in the surface exposure of lumenal epitopes of SV proteins. We have recently described the use of antibodies directed against the lumenal N-terminus of synaptotagmin I (Sytlum-Abs) to morphologically monitor exo-endocytic recycling of SVs. We report here that a radioimmunoa...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:The neonatal heart is a very useful tool for the study of biochemical pathways and properties of cardiomyocytes and as it has the potential to regenerate for a brief period of time from birth; it is also useful to study cardiac regeneration. However, as the heart matures, this proficiency for regeneration is reduced. ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2015.06.009
更新日期：2015-10-01 00:00:00
abstract：:The high-affinity receptor (Fc gamma RI) for the constant (Fc) portion of immunoglobulin G (IgG) is one of three Fc IgG receptor classes (Fc gamma Rs) found on mononuclear phagocytes. The functional specialization of each of the Fc gamma R classes is not well understood. Previous studies utilizing anti-Fc gamma R mono...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Cytolocalization of stomatin, an integral membrane protein also called erythrocyte band 7.2b, was investigated in a human epithelial cell line in which the expression of this protein is up-regulated after treatment with interleukin-6 and dexamethasone. A monoclonal antibody against stomatin was used to perform immunof...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:We have examined the polarity of microtubules in Sertoli cells of the seminiferous epithelium during testicular development to test the hypothesis that microtubules change their polarity during tight junction formation. Microtubules in a number of polarized epithelial cells, including Sertoli cells, are oriented with ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
