Abstract:
:The differences in the content of trace elements (Fe, Cu, Zn, Br, Rb and Sr) in phasic and tonic scallop adductor muscles have been revealed by using X-ray fluorescence analysis. The phasic (yellow) muscles have been found to be richer in Fe and Cu while more deficient in Zn and Br as compared to the tonic (white) muscles. No appreciable differences in the Rb and Sr content has been found. A statistical treatment of the data obtained suggests that phasic and tonic muscles differ in their metabolism of trace elements and the regulation of the concentrations of various trace elements by the muscle cell is achieved by different mechanisms.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Ogievetskaya MM,Maslov O,Sheludko Nsubject
Has Abstractpub_date
1981-04-01 00:00:00pages
124-7issue
1eissn
0171-9335issn
1618-1298journal_volume
24pub_type
杂志文章abstract::Isolated microtubule protein from axopodia of the heliozoan Echinosphaerium nucleofilum, consisting of two major bands on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), has been compared to axonemal and cytoplasmic tubulins from both animal and non-animal sources. The upper E. nucleofilum protein band migrated fas...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract::HeLa cells were treated with different concentrations of an inhibitor of the proteasome chymotrypsin-like activity, the peptidyl aldehyde N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (PSI). A detailed analysis, which included flow cytometry, cell counting and morphological assessment, was performed. PSI treatme...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::Mouse plasma cells have been exposed to a wide range of soluble and adsorptive macromolecular tracers for 10 min to 4 h to explore the possibility of membrane recycling related to the high secretory rate of these nonregulated secretory cells. Electron microscopic examination showed in all cases labeling of multivesicu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::In recent years, several antagonists of alpha(v)beta3 have been used to develop therapeutic approaches to the treatment of melanoma neoplasia. We studied the effects of anti-alpha(v)-integrin-blocking antibodies on attached M21 melanoma cells, the cellular distribution of alpha(v)-integrin and the molecular organizati...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00067
更新日期:2000-07-01 00:00:00
abstract::We examined the Ca2+ handling property and cell function of CCL39 fibroblasts highly overexpressing the cardiac isoform (NCX1) of Na+/ Ca2+ exchanger. In NCX1 transfectants in 146 mM Na+, ionomycin, alpha-thrombin or thapsigargin only produced a small transient increase in [Ca2+]i compared to the large increase seen i...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80038-1
更新日期:1998-07-01 00:00:00
abstract::Cartilage matrix protein (CMP), a major noncollagenous component of certain types of hyaline cartilage, is synthesized by chondrocytes in a developmentally regulated manner. In this study, we monitored the accumulation of CMP in the developing chicken limb and sternum by immunostaining. In older embryos, the specific ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::Cancer/testis antigens (CTAs) are characterized by their restricted expression pattern. In normal individuals their expression is largely restricted to the testis. In the case of cancer patients, CTA expression has also been frequently observed in the tumoral cells. CTAs are considered to be promising targets for immu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2004.11.003
更新日期:2005-03-01 00:00:00
abstract::Lysosomal Ca2+ release channel TRPML1 has been suggested to regulate lysosome size by activating calmodulin (CaM). To further understand how TRPML1 and CaM regulate lysosome size, in this study, we report that inhibiting mTORC1 causes enlarged lysosomes, and the recovery of enlarged lysosomes is suppressed by inhibiti...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2019.05.001
更新日期:2019-06-01 00:00:00
abstract::This paper explores the mode of action of the tranquillizers chloral hydrate and diazepam during fertilization and mitosis of the first reproductive cell cycles in sea urchin eggs. Most striking effects of these drugs are the alteration of centrosomal material and the abnormal microtubule configurations during exposur...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(98)80041-1
更新日期:1998-01-01 00:00:00
abstract::Chlorpromazine (CPZ) at minimally effective concentrations accumulates mammalian cells in mitosis without lethal effects on the cells. Star-metaphase morphology similar to effects seen with classical antimitotic compounds probably results from the preferential action of CPZ on a specific class of microtubules--the pol...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::A three-dimensional cytoskeleton, morphologically composed of intermediate filaments, desmosomes, and Z-disks, remains in bovine heart Purkinje fibres after extraction with Triton X-100 and low and high ionic strength solutions. Biochemically this cytoskeleton mainly consists of skeletin (Mr approximately 55 000) and ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
abstract::The costae are cytoskeletal structures found in Trichomonadidae. Both the structural organization and composition of this organelle are still unknown. In the present work we have introduced a new methodology for the costa isolation. Using sucrose density-gradient centrifugation an enriched costa fraction was obtained....
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-04-01 00:00:00
abstract::The yeast dynamin Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains u...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.02.004
更新日期:2017-03-01 00:00:00
abstract::Proteins that are concentrated in specific compartments of the endomembrane system in order to exert their organelle-specific function must possess specific localization signals that prevent their transport to distal regions of the exocytic pathway. Some resident proteins of the endoplasmic reticulum (ER) that are kno...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/S0171-9335(04)70025-4
更新日期:2000-04-01 00:00:00
abstract::Conventional fixation with glutaraldehyde fixatives at pH 7.4 did not preserve/promote thick filaments in Bufo smooth muscle. However, if pH was lowered to 6.0, thick filaments were present and addition of dimethyl sulfoxide (DMSO) led to a greater number of thick filaments. Stretch alone had little effect on the pres...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
abstract::Filamentous networks were isolated from rat liver and studied by electron microscopy after negative staining with uranyl acetate. Highly purified preparations of networks were used to obtain material for immunological studies, but morphological studies were made more satisfactorily on preparations purified only by suc...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::The major families of proteoglycans in human arterial tissue have been localized and characterized by electron microscopy. After staining with the polycationic dye cuprolinic blue in the presence of a critical electrolyte concentration, three differently sized populations of proteoglycan-cuprolinic blue precipitates a...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::The spatial and temporal distribution as well as ultrastructural and biochemical characteristics of apoptotic and mitotic cells during human eye development were investigated in 14 human conceptuses of 4-9 postovulatory weeks, using electron and light microscopy. In the 5th developmental week, apoptotic and mitotic ce...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00328
更新日期:2003-08-01 00:00:00
abstract::Recently, we have described the developmental expression of the small heat shock proteins (sHsps) Hsp27/HspB1 and alphaB-crystallin/HspB5 in different tissues of pigs from almost full-term foetuses to three years old adults (P. Tallot, J. F. Grongnet, J. C. David, Biol. Neonate, 83, 281-288, 2003). The data described ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00337
更新日期:2003-10-01 00:00:00
abstract::We have investigated the effects of bafilomycin A1, a specific inhibitor of vacuolar type H(+)-ATPase, on the secretion and intracellular distribution of prolactin in cultured rat pituitary tumor cells (GH3 cells). Pulse-labeling the cells with L-[35S]methionine for 5 min and subsequently incubating in chase medium co...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
abstract::Advanced therapies in medicine use stem cells, gene editing, and tissues to treat a wide range of conditions. One of their goals is to stimulate endogenous repair of tissues and organs by manipulating stem cells and their niche, as well as to optimize the intrinsic characteristics and plasticity of differentiated cell...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2020.151123
更新日期:2020-09-01 00:00:00
abstract::The presence of a filamin-like protein in oxyntic cells was established by indirect immunofluorescence microscopy. The location of this protein and myosin was studied, using specific antibodies, on frozen sections and isolated cells. Antifilamin and antimyosin reacted strongly with the luminal cytoplasm of the cells. ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::Despite its frequent inactivation in human breast cancers, the role of p21(Cip1) (p21) in morphological plasticity of normal mammary epithelial cells is still poorly understood. To address this question, we have investigated the consequences of p21 silencing in two-dimensional (2D) morphogenesis of untransformed human...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2011.03.002
更新日期:2011-08-01 00:00:00
abstract::Exocytosis is mediated by high-affinity interactions between different SNARE proteins. The existence of several variants of each SNARE protein suggests that the specificity of fusion may be directed by unique combination of SNARE family members. We examined if two alternatively spliced variants of synaptosomal-associa...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00106
更新日期:2000-11-01 00:00:00
abstract::The hexameric ATPase p97/yeast Cdc48p has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. p97/Cdc48p contains two conserved consensus p34cdc2 kinase phosphorylation sites with...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/S0171-9335(99)80055-7
更新日期:1999-04-01 00:00:00
abstract::Epidermal Langerhans cells (LC) play a key role in initiation and regulation of immune responses. Whereas LC migration out of the epidermis upon environmental assault is extensively studied, the mechanisms involved in the (re)population of the epidermis with LC are poorly understood. Here, we investigated the immigrat...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2012.06.004
更新日期:2012-10-01 00:00:00
abstract::The trafficking of intracellular membranes requires the coordination of membrane-cytoskeletal interactions. Rab proteins are key players in the regulation of vesicular transport, while Rho family members control actin-dependent cell functions. We have previously identified a rho protein, rhoD, which is localized to th...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00173
更新日期:2001-06-01 00:00:00
abstract::The outer dense fiber protein ODF2 is the major component of the sperm tail cytoskeleton and a critical component of the mature centriole of the centrosome. Centriole maturation involves the formation of appendages and the recruitment of ODF2/Cenexin. ODF2 and Cenexin are alternative splice variants that differ in a s...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2007.10.004
更新日期:2008-03-01 00:00:00
abstract::The release of newly synthesized neuropeptides was studied in an in vitro system using the adipokinetic hormone (AKH)-producing cells of an insect (Locusta migratoria) as a model system. Tritiated phenylalanine incorporated into three hormonal neuropeptides, AKH I, II and III, was used to distinguish newly synthesized...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::The subcellular site of accumulation of non-esterified cholesterol and sphingomyelin in lipid-laden livers and spleens from rats given multiple intravenous injections with liposomes made up of these lipids were investigated by morphological and biochemical techniques. The subcellular fractionation of liver homogenates...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00