Abstract：
:A three-dimensional cytoskeleton, morphologically composed of intermediate filaments, desmosomes, and Z-disks, remains in bovine heart Purkinje fibres after extraction with Triton X-100 and low and high ionic strength solutions. Biochemically this cytoskeleton mainly consists of skeletin (Mr approximately 55 000) and of alpha-actinin (Mr approximately 95 000). Minor high molecular weight components are also present. By gel electrophoresis-derived enzyme-linked immunosorbent assay (GEDELISA) we show here that a 150 000-dalton component is a complex of alpha-actinin and skeletin. Furthermore, this 150 000-dalton component has now been purified and can be converted into 95 000- and 55 000-dalton subunits by reduction with a high concentration of beta-mercaptoethanol and heating. We also show that solubilized pure skeletin and pure alpha-ctinin from Purkinje fibres, after mixing, partly form a stable complex with a molecular weight of about 150 000 daltons.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Kjörell U,Thornell LEsubject
Has Abstractpub_date
1982-08-01 00:00:00pages
139-44issue
1eissn
0171-9335issn
1618-1298journal_volume
28pub_type
杂志文章abstract：:The interaction of daunomycin (DAU), an anthracyclinic antibiotic employed as antitumoral agent, with microtubules, has been investigated by cytochemical and morphological methods on a human melanoma cell line (H14). Results obtained indicated that DAU was able to modulate the microtubule reassembly in cells treated w...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:The weak bases chloroquine, primaquine, NH4Cl and the ionophore monensin exert similar but not identical effects on sorting, transport and processing of cathepsin D in several human cell lines (fibroblasts, HepG2 cells, U937, monocytes). The drugs inhibit the segregation of newly synthesized cathepsin D from the secre...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequen...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:The aim of this study was to evaluate the influence of peroxisome proliferator-activated receptor γ (PPARγ) on self-renewal of mouse embryonic stem cells (mESCs) in the presence and absence of leukemia inhibitory factor (LIF). We demonstrated that in the presence of LIF, the activation of PPARγ by Rosiglitazone led to...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2013.03.003
更新日期：2013-04-01 00:00:00
abstract：:Formation of the complete spindles during the budding process of Saccharomyces uvarum was investigated by fluorescence microscopy of protoplasted cells. Protoplasts were treated with anti-tubulin antibodies and DAPI, a fluorescent dye staining DNA. Thus, both chromatin and spindles could be visualized. Duplication as ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Connexins26 and -32 are subunit proteins of gap junctions that are coexpressed in hepatocytes and several tissues but individually expressed in other cells. Molecular cloning of both corresponding mouse genes revealed similar genomic organization, i.e., each gene consists of two exons with the complete coding region l...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:In the presence of various commonly used buffers, phosphate-buffered saline (PBS), tris-buffered saline (TBS), Na-cacodylate, bovine serum albumin and a wide range of cytochemically active proteins (monoclonal and polyclonal IgG, concanavalin A, Ricinus communis lectin I, Helix pomatia lectin, protein A) were complexe...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:In the oil containing spores of Bryum capillare, lipid breakdown occurs within three days after sowing. The periods of activity of acid, neutral and alkaline lipases are coincident. They start at the first day after sowing the spores. After 3 days of germination, when storage fats forming large globules are mobilised ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:To study molecules secreted from cultured plant cells that promote development, maize microspores were transferred into culture and the conditioned media were collected over time and analysed. Electrophoresis indicated that both non-glycosylated and glycosylated proteins including arabinogalactan proteins (AGPs) appea...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00378
更新日期：2004-07-01 00:00:00
abstract：:Specific antibodies to a protein designated P1 (Mr approximately equal to 63,000), which is specifically altered in mutants resistant to the microtubule inhibitor podophyllotoxin, bind to mitochondria in cells of various vertebrate and invertebrate species (Eur. J. Cell Biol. 44, 278-285 (1987); Can. J. Biochem. Cell ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
abstract：:Myeloid hematopoietic precursor cells are induced to differentiate by the macrophage and granulocyte differentiation-inducing protein MGI-2 (DF). This differentiation-inducing protein bound to double-stranded but not to single-stranded mammalian DNA. The bound MGI-2 was not eluted by high salt, but was eluted by sodiu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:The organelles of the endocytic and autophagic pathways were studied in HeLa cells using immunoelectron microscopy and stereological techniques. In the absence of autophagic stimulation, characteristic particulate structures containing closely packed layers of membrane received the fluid-phase marker horseradish perox...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-04-01 00:00:00
abstract：:It is generally believed that the induction of actin cytoskeleton rearrangements by extracellular stimuli results from the activation of guanine nucleotide exchange factors for the Rho GTPases. Here, we present evidence that the inactivation of RhoGAP (GTPase activating protein) activity is an equally effective means ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80019-3
更新日期：1999-08-01 00:00:00
abstract：:1. Feeding weanling mice a diet containing 0.5% (w/w) cuprizone (biscyclohexanone oxaldihydrazone) induced megamitochondria of a different type of that previously described (Suzuki, K., Science 163, 81-82 (1969). 2. As in the normal controls, a single population of mitochondria with a narrow size distribution, was det...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:Hyaluronan is a straight chain, glycosaminoglycan polymer of the extracellular matrix composed of repeating units of the disaccharide [-D-glucuronic acid-beta1,3-N-acetyl-D-glucosamine-beta1,4-]n. Hyaluronan is synthesized in mammals by at least three synthases with products of varying chain lengths. It has an extraor...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2006.05.009
更新日期：2006-08-01 00:00:00
abstract：:Here, we report the establishment of a permanent skin cell culture from rainbow trout (Oncorhynchus mykiss). The cells of the fish skin cell culture could be propagated over 60 passages so far. Furthermore, we show for the first time that it is possible to integrate freshly harvested rainbow trout scales into this new...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2011.08.003
更新日期：2011-12-01 00:00:00
abstract：:RNA interference (RNAi) is a powerful tool for functional genetic studies in model organisms and mammalian cells. To facilitate rapid construction of gene knockdown constructs and RNAi libraries for known genes of mammalian cells, a new and simple strategy to produce small interfering RNA (siRNA) expression vectors wi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2005.10.009
更新日期：2006-05-01 00:00:00
abstract：:We have measured the rate of secretion in vitro of individual rat exocrine pancreatic enzymes and proenzymes from cells in pancreatic lobules of control animals and animals subjected to 24 h optimal hormonal prestimulation with cerulein, in vivo. With the controls secreted proteins were first detectable at 20 to 25 mi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:The high-affinity receptor (Fc gamma RI) for the constant (Fc) portion of immunoglobulin G (IgG) is one of three Fc IgG receptor classes (Fc gamma Rs) found on mononuclear phagocytes. The functional specialization of each of the Fc gamma R classes is not well understood. Previous studies utilizing anti-Fc gamma R mono...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Herein we report that Gts1p fused with green-fluorescent protein (GFP) is localized in the cortical actin patch besides nuclei in yeast and the cortical Gts1p changed its position together with the patch depending on the cell-cycle phase, while nuclear Gts1p accumulated predominantly in the budding phase. Whereas Gts1...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2007.02.005
更新日期：2007-05-01 00:00:00
abstract：:Mouse epidermal melanoblasts preferentially proliferated from disaggregated epidermal cell suspensions derived from newborn mouse skin in serum-free medium (MPM) supplemented with dibutyryl adenosine 3', 5' cyclic monophosphate (DBcAMP) and basic fibroblast growth factor (bFGF). After 12 days, almost all keratinocytes...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Monensin, a monovalent ionophore, caused swelling of mature cisternae of plant Golgi apparatus. The appearance of swollen cisternae was time-dependent and linear over a period of 1 h with an estimated maximum rate of production of one swollen cisterna every 3 to 4 min. Implicit in these observations was a need for the...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:A novel cytochemical method for the in situ, ultrastructural localization of phospholipids in biological tissues is reported. The method is based on the enzyme-gold approach (M. Bendayan: J. Histochem. Cytochem. 29, 531, 1981). Phospholipase A2 from bee venom was adsorbed on colloidal gold particles (PLA2-gold) and ap...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:Epidermal Langerhans cells (LC) play a key role in initiation and regulation of immune responses. Whereas LC migration out of the epidermis upon environmental assault is extensively studied, the mechanisms involved in the (re)population of the epidermis with LC are poorly understood. Here, we investigated the immigrat...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2012.06.004
更新日期：2012-10-01 00:00:00
abstract：:Actin, myosin, and the actin-associated proteins tropomyosin, alpha-actinin, vinculin, and villin were localized in acinar cells of rat and bovine pancreas, parotid, and prostate glands by means of immunofluorescent staining of both frozen tissue sections and semithin sections of quick-frozen, freeze-dried, and plasti...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:In the cell cycle the transition from G2 phase to cell division (M) is strictly controlled by protein phosphorylation-dephosphorylation reactions effected by several protein kinases and phosphatases. Although much indirect and direct evidence point to a key role of protein phosphatase 2A (PP2A) at the G2/M transition,...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2005.04.002
更新日期：2005-08-01 00:00:00
abstract：:The macronuclear DNA synthesis in well-fed carnivorous forms of Tetrahymena paravorax has been investigated with autoradiographic experiments. The macrostome cell type of this ciliate is induced by stomatin and fed on living prey (T. pyriformis). The DNA synthesis is re-initiated when the macrostomes become active pre...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-06-01 00:00:00
abstract：:ATP-dependent interactions between myosin and actin in the lower eukaryote, Physarum polycephalum, are inhibited by micromolar levels of Ca2+. This inhibition is mediated by the binding of Ca2+ to myosin, the phosphorylation of which is required if Ca2+ is to inhibit the activities of myosin (Kohama, K., Trends Pharma...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:PinX1 was originally identified as a Pin2/TRF1-interacting protein that suppresses telomerase activity via its telomerase inhibitor domain (TID) and regulates the nucleolar localization of TRF1 in telomerase-positive cells. In addition to its telomeric localization, PinX1 can be found in the nucleoli of human cells. O...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.05.005
更新日期：2010-10-01 00:00:00
abstract：:Changes in the surface morphology of regenerating rabbit, rat and frog corneal endothelial cells in vivo have been investigated by scanning electron microscopy. In adult tissue these cells do not normally divide unless given a stimulus, such as injury. Surfaces of quiescent rabbit and rat cells are devoid of microvill...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
