Abstract：
:To study molecules secreted from cultured plant cells that promote development, maize microspores were transferred into culture and the conditioned media were collected over time and analysed. Electrophoresis indicated that both non-glycosylated and glycosylated proteins including arabinogalactan proteins (AGPs) appeared in the medium and their concentration increased during the time of culture. The development of embryos was correlated with the presence of specific extracellular proteins, using an experimental system based on a tunicamycin inhibition test. In addition, a precise protein analysis was conducted using MALDI-TOF and ESI-MS-MS techniques. These approaches have allowed the identification of 5 other types of proteins: a cell wall invertase, two thaumatin isoforms, one 1-3 beta-glucanase and two chitinase isoforms. Altogether these experiments and results open ways for research aimed at understanding which molecules stimulate embryo formation. Moreover, AGPs may be used to stimulate the development of microspores (pollen embryogenesis) prepared from non-responsive genotypes.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Borderies G,le Béchec M,Rossignol M,Lafitte C,Le Deunff E,Beckert M,Dumas C,Elisabeth MRdoi
10.1078/0171-9335-00378subject
Has Abstractpub_date
2004-07-01 00:00:00pages
205-12issue
5eissn
0171-9335issn
1618-1298pii
S0171-9335(04)70358-1journal_volume
83pub_type
杂志文章abstract：:MUC16/CA125 is over-expressed in human epithelial tumors including ovarian, breast and some other carcinomas. The purpose of this study is to investigate how cell surface MUC16 is functionally involved in tumor progression, with a special focus on the role of its cytoplasmic tail. Forced expression of C-terminal MUC16...
journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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更新日期：1997-11-01 00:00:00
abstract：:Chondrocyte-matrix interaction is mediated by a series of adhesion molecules. Both alpha and beta integrin subunits are involved and govern crucial functions of cell adhesion and signal transduction. These molecules modulate proliferation and differentiation, thus establishing cartilage integrity. We studied the influ...
journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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更新日期：1985-05-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1983-03-01 00:00:00
abstract：:Human enteric α-defensins (HD5 and HD6), major antimicrobial peptides produced by Paneth cells in the intestine, play important roles in intestinal innate immunity. Since their expression is decreased in Crohn's disease (CD), with decreased expression being more pronounced in the presence of NOD2 mutations, it would b...
journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1997-02-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
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更新日期：1987-12-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-09-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1985-05-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
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journal_title：European journal of cell biology
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doi：
更新日期：1980-08-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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doi：
更新日期：1986-03-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1983-01-01 00:00:00
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
