Abstract：
:In the oil containing spores of Bryum capillare, lipid breakdown occurs within three days after sowing. The periods of activity of acid, neutral and alkaline lipases are coincident. They start at the first day after sowing the spores. After 3 days of germination, when storage fats forming large globules are mobilised no lipase activity could be detected any longer in the glyoxysomes. Acid lipase is preferentially located in spherosomal membrane but is also present at the glyoxysomal membrane. Alkaline lipase is specifically located in the glyoxysomes and mitochondria. A cooperation presumably exists between acid and alkaline lipases in the hydrolysis of monoglycerides at the glyoxysomal level. Neutral lipase activity is localized at the spherosomal membrane, in the endoplasmic reticulum and in dictyosomes. It is absent from glyoxysomes.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Carrapiço F,Salomé M,Pais Ssubject
Has Abstractpub_date
1983-03-01 00:00:00pages
42-6issue
1eissn
0171-9335issn
1618-1298journal_volume
30pub_type
杂志文章abstract：:Dictyosomes, Golgi vesicles, and plasma membranes were investigated after freeze-fracture in cells from growing root tips of cress (Lepidium sativum L.), that are distinguishable by different cellulose content of the cell wall, into (i) meristematic cells during early formation of the cell plate, (ii) statocytes of th...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Application of carbon tetrachloride produced a progressive proliferation of tight junctions in the rat liver. This system proved to be rapid and highly reproducable and affords the opportunity for tracing the fate of tight junctions in freeze-fracture replicas, facilitating investigations on their formation and functi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:Centaurin alpha-1 is a high-affinity PtdIns(3,4,5)P3-binding protein enriched in brain. Sequence analysis indicates centaurin alpha-1 contains two pleckstrin homology domains, ankyrin repeats and an Arf GAP homology domain, placing it in the AZAP family of phosphoinositide-regulated Arf GAPs. Other members of this fam...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00416
更新日期：2004-10-01 00:00:00
abstract：:Malignant melanoma is an aggressive form of skin cancer with an increasing incidence worldwide. One way to address the pathology of the disease is through molecular research. In addition to the analysis of melanoma-relevant signaling pathways, the investigation of important transcription factors is a fundamental objec...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2013.10.003
更新日期：2014-01-01 00:00:00
abstract：:A state of permanent growth arrest characterises a senescent cell. Both the beneficial and deleterious effects that have accrued in senescent cells are observed in a complex organ, such as the liver. Injury to liver tissues triggers processes of regeneration and associated wound healing. Persistent injury can also lea...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2012.08.002
更新日期：2012-10-01 00:00:00
abstract：:Next to the already available mouse monoclonal and laboratory animal-derived polyclonal antibodies, recombinant antibodies offer an additional and virtually unlimited arsenal of new immunohistochemical research tools. The major advantages of recombinant antibodies are their rapid and easy generation against virtually ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2004.12.025
更新日期：2005-04-01 00:00:00
abstract：:Invadopodia are actin-rich, adhesive protrusions that extend into and remodel the extracellular matrix. They are associated with high levels of pericellular proteolysis and correlate with the invasive capacity of a variety of tumour cells. Invadopodia have, thus, been proposed to recapitulate key events of the metasta...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2010.04.007
更新日期：2011-02-01 00:00:00
abstract：:Dipyridamole (DPD), a widely used anti-thrombotic agent, inhibits in vitro directional migration of 10T1/2 mouse embryo cells in a dose dependent way. The inhibition is detectable at DPD concentrations comparable to those in human plasma after oral intake of the drug. Using a model for quantitative shape change analys...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:Direct conversion by overexpression of defined transcription factors (TFs) is a promising new method that can generate desired cell types from abundant, accessible cells. While previous studies have reported hepatic generation from fibroblasts, tremendous interest exists in the understanding of hepatic reprograming an...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2015.10.003
更新日期：2015-12-01 00:00:00
abstract：:The AtT20 pituitary cell is the one that was originally used to define the pathways taken by secretory proteins in mammalian cells. It possesses two secretory pathways, the constitutive for immediate secretion and the regulated for accumulation and release under hormonal stimulation. It is in the regulated pathway, mo...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00365
更新日期：2004-04-01 00:00:00
abstract：:This paper explores the mode of action of the tranquillizers chloral hydrate and diazepam during fertilization and mitosis of the first reproductive cell cycles in sea urchin eggs. Most striking effects of these drugs are the alteration of centrosomal material and the abnormal microtubule configurations during exposur...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80041-1
更新日期：1998-01-01 00:00:00
abstract：:PinX1 was originally identified as a Pin2/TRF1-interacting protein that suppresses telomerase activity via its telomerase inhibitor domain (TID) and regulates the nucleolar localization of TRF1 in telomerase-positive cells. In addition to its telomeric localization, PinX1 can be found in the nucleoli of human cells. O...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.05.005
更新日期：2010-10-01 00:00:00
abstract：:Mitotic PtK1 spindles were UV irradiated (285 nm) during metaphase and anaphase between the chromosomes and the pole. The irradiation, a rectangle measuring 1.4 x 5 microns parallel to the metaphase plate, severed between 90 and 100% of spindle microtubules (MTs) in the irradiated region. Changes in organization of MT...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:The P446L mutant Drosophila importin-beta (P446L-imp-beta) has been reported to prohibit--in dominant negative fashion--nuclear envelope (NE) assembly. Along elucidating the mode of action of P446L-imp-beta we studied in vitro NE assembly on Sepharose beads. While Drosophila embryo extracts support NE assembly over Se...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00324
更新日期：2003-07-01 00:00:00
abstract：:To investigate the association of calmodulin (CaM) with microtubules (MTs) in the mitotic apparatus (MA), the distributions of both CaM and tubulin were examined in mitotic PtK1 cells in which MT subclasses had been selectively removed or altered by treatment with cold or with the MT inhibitor, nocodazole. A fluoresce...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:Coexistence of four different forms of chromatin was observed by electron microscopy in nuclear spread preparations of monkey kidney cells during late stages of infection with herpes simplex virus (HSV-1 AMG). Besides typical nucleosomal (i) chromatin, thin (3-5 nm) strands morphologically indistinguishable from prote...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:Earlier studies have indicated that Jun/AP-1 activity is associated with, and probably required for apoptosis induction by DNA-damaging and stress-inducing agents in human myeloid cells. To investigate this possibility, we examined the capacity of continuous treatments with etoposide (10 microM) and camptothecin (0.4 ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/S0171-9335(04)70001-1
更新日期：2000-01-01 00:00:00
abstract：:Embryonic stem (ES) cells are omnipotent; they can differentiate into every cell type of the body. The development of culture conditions that allow their differentiation has made it conceivable to produce large numbers of cells with lineage-specific characteristics in vitro. Here, we describe a method by which murine ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2007.07.001
更新日期：2007-12-01 00:00:00
abstract：:The mutation raised (rsd, 3-95.4) of Drosophila melanogaster causes flightlessness as a consequence of abnormalities in the fibrillar flight muscles (FFMs). In this muscle type actin III is neither synthesized nor accumulated while adult tubular muscles of rsd flies are indistinguishable from wildtype. This paper demo...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:The macronuclear DNA synthesis in well-fed carnivorous forms of Tetrahymena paravorax has been investigated with autoradiographic experiments. The macrostome cell type of this ciliate is induced by stomatin and fed on living prey (T. pyriformis). The DNA synthesis is re-initiated when the macrostomes become active pre...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-06-01 00:00:00
abstract：:The Semper cells in the ommatidia of the blowfly contain an impressive cytoskeleton consisting of hundreds of microtubules. The microtubules are intimately interwoven by an extensive system of the smooth endoplasmic reticulum. The cells were permeabilized by saponin treatment, subsequently incubated in a loading mediu...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:The monoclonal antibody MPM-12, raised by using partially purified extract of mitotic HeLa cells as the immunogen, preferentially stains the cytoplasm of mitotic cells by indirect immunofluorescence without exhibiting any species specificity. On immunoblots, MPM-12 recognizes three bands, of 155, 88, and 68 kDa, in mi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:The transcriptional status of cells can be deduced from the staining pattern of various nuclear markers such as the Cajal body, nucleolus and nuclear speckles. In this study we have used these markers to correlate transcriptional status with cell differentiation in the lens. As a closed system with no cell loss and wi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00275
更新日期：2002-10-01 00:00:00
abstract：:The milk yeast Kluyveromyces lactis is an alternative model yeast to the well established Saccharomyces cerevisiae. The cell wall of these fungi consists of polysaccharides (i.e. long chains of β-1,3- and β-1,6-linked sugar chains and some chitin) and mannoproteins, both of which are continually adapted to environment...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2011.04.005
更新日期：2011-09-01 00:00:00
abstract：:In Physarum polycephalum (Myxomycetes) aphidicolin has been found to delay metaphase onset when applied to synchronous plasmodia 3 h before control metaphase. In contrast to the action of temperature shifts, aphidicolin treatment did not delay the initiation of the increase of thymidine kinase synthesis (EC 2.7.1.21, ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:Oocyte-thymocyte mouse cell hybrids were produced using polyethylene glycol (PEG) and examined at the ultrastructural level. Fusion was accomplished either before or after activation of metaphase II oocytes. In both experimental variants thymocyte nuclei undergo remodelling which comprises the following sequence of ev...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Connexins26 and -32 are subunit proteins of gap junctions that are coexpressed in hepatocytes and several tissues but individually expressed in other cells. Molecular cloning of both corresponding mouse genes revealed similar genomic organization, i.e., each gene consists of two exons with the complete coding region l...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:The microtubule-associated protein 2 (MAP2) and its juvenile splicing variant MAP2c contain a phosphorylation site at Ser136 which is part of a Ser-Pro motif. This site lies within the N-terminal region common to MAP2b and MAP2c. It has been mapped by site-directed mutagenesis of recombinant MAP2c and by a monoclonal ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Involvement of CDK2 in glucocorticoid-mediated S-phase lengthening was analyzed in this work. Dexamethasone (DXM) treatment of PHA-stimulated lymphocytes induced a decrease in CDK2 mRNA expression without any change in mRNA stability. This glucocorticoid-induced decrease in CDK2 mRNA expression could be suppressed by ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00298
更新日期：2003-05-01 00:00:00
abstract：:Programmed cell death (PCD) is the regulated removal of cells within an organism and plays a fundamental role in growth and development in nearly all eukaryotes. In animals, the model organism Caenorhabditis elegans (C. elegans) has aided in elucidating many of the pathways involved in the cell death process. Various ...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2012.02.002
更新日期：2012-08-01 00:00:00
