Abstract:
:The P446L mutant Drosophila importin-beta (P446L-imp-beta) has been reported to prohibit--in dominant negative fashion--nuclear envelope (NE) assembly. Along elucidating the mode of action of P446L-imp-beta we studied in vitro NE assembly on Sepharose beads. While Drosophila embryo extracts support NE assembly over Sepharose beads coated with Ran, NE assembly does not take place in extracts supplied with exogenous P446L-imp-beta. A NE also forms over importin-beta-coated beads. Surprisingly, when immobilized to Sepharose beads P446L-imp-beta as efficiently recruits NE vesicles as normal importin-beta. The discrepancy in behavior of cytoplasmic and bead-bound P446L-imp-beta appears to be related to icreased--as compared to normal importin-beta--microtubule (MT) binding ability of P446L-imp-beta. While wild-type importin-beta is able to bind MTs and the binding decreases upon RanGTP interaction, P446L-imp-beta cannot be removed from the MTs by RanGTP. P446L-imp-beta, like normal importin-beta, binds some types of the nucleoporins that have been known to be required for NE assembly at the end of mitosis. It appears that the inhibitory effect of P446L-imp-beta on NE assembly is caused by sequestering some of the nucleoporins required for NE assembly to the MTs.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Tirián L,Timinszky G,Szabad Jdoi
10.1078/0171-9335-00324subject
Has Abstractpub_date
2003-07-01 00:00:00pages
351-9issue
7eissn
0171-9335issn
1618-1298pii
S0171-9335(04)70306-4journal_volume
82pub_type
杂志文章abstract::Hydrogencarbonate and chloride activated, ouabain-insensitive ATPase activities are demonstrated in the salt-absorbing rectum of larval dragonflies. Maximal activation is achieved at approx. 30 mM HCO3- and 20 mM Cl-, respectively. The stimulation of each anion obeys Michaelis-Menten kinetics Km values are 4.65 mM for...
journal_title:European journal of cell biology
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doi:
更新日期:1980-02-01 00:00:00
abstract::Dermatological diseases range from minor cosmetic problems to life-threatening conditions, as seen in some severe disorders of keratinization and cornification. These disorders are commonly due to abnormal epidermal differentiation processes, which result in disturbed barrier function of human skin. Elucidation of the...
journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00
abstract::Specific rabbit antisera were prepared against purified aggregation factor and its membrane-associated receptor, baseplate, derived from the marine sponge. Microciona prolifera. They were utilized in conjunction with fluorescent-labeled goat anti-rabbit IgG in an assay to demonstrate the surface localizations of both ...
journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1980-12-01 00:00:00
abstract::Embryonic chick myoblasts possess an extensive network of cytoplasmic microtubules which emanate from a single, perinuclear centrosome containing a microtubule-organizing center (MTOC) and the centrioles. However, after myoblasts fuse into myotubes the centrosome is no longer apparent, and instead long parallel arrays...
journal_title:European journal of cell biology
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更新日期:1986-01-01 00:00:00
abstract::Application of carbon tetrachloride produced a progressive proliferation of tight junctions in the rat liver. This system proved to be rapid and highly reproducable and affords the opportunity for tracing the fate of tight junctions in freeze-fracture replicas, facilitating investigations on their formation and functi...
journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1979-10-01 00:00:00
abstract::Dictyosomes, Golgi vesicles, and plasma membranes were investigated after freeze-fracture in cells from growing root tips of cress (Lepidium sativum L.), that are distinguishable by different cellulose content of the cell wall, into (i) meristematic cells during early formation of the cell plate, (ii) statocytes of th...
journal_title:European journal of cell biology
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更新日期:1983-05-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1982-04-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1988-08-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1986-10-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
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journal_title:European journal of cell biology
pub_type: 杂志文章
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journal_title:European journal of cell biology
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更新日期:1997-12-01 00:00:00
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journal_title:European journal of cell biology
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doi:
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
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journal_title:European journal of cell biology
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doi:
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doi:
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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