Abstract:
:Etoposide induced a megabase (Mb) fragmentation pattern identical with that from genomic digestion by NotI restriction endonuclease which specifically cleaves CpG islands in euchromatin domains. Redigestion by NotI produced no change, suggesting cleavage in the same or closely related sites in euchromatin domains. Preferential euchromatin cleavage was further suggested by harvested metaphase chromosomes showing self-inflicted resolution of light G-bandings (R-bandings), the euchromatin domains. Autodegeneration following Mb euchromatin fragmentations was shown by their degradation into 200 bp ladders, and expressions of apoptotic and "non-apoptotic" active death morphologies that were also seen conjointly in the same cell. The endstage further showed heterochromatin masses anchored to the nucleolus by novel ball-and-socket joints where dislocations occurred with nuclear leakage.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Qi L,Sit KHdoi
10.1016/S0171-9335(98)80112-Xsubject
Has Abstractpub_date
1998-11-01 00:00:00pages
239-46issue
3eissn
0171-9335issn
1618-1298pii
S0171-9335(98)80112-Xjournal_volume
77pub_type
杂志文章abstract::Many hormones/factors influence the total body growth and development during embryonic life, and very few studies have been carried out to ascertain their effects on the individual organ system. In this study, the effect of exogenous insulin-like growth factor-I (IGF-I) on embryonic kidneys was investigated, and corre...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::Epidermal Langerhans cells (LC) play a key role in initiation and regulation of immune responses. Whereas LC migration out of the epidermis upon environmental assault is extensively studied, the mechanisms involved in the (re)population of the epidermis with LC are poorly understood. Here, we investigated the immigrat...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2012.06.004
更新日期:2012-10-01 00:00:00
abstract::HeLa cells growing in vitro were treated with the peptidyl aldehyde inhibitor of the chymotrypsin-like activity of the proteasome N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (PSI). Immunofluorescence studies of treated cells revealed the formation of massive perinuclear aggregates rich in ubiquitin and proteas...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::The realization that a highly conserved family of membrane proteins are localized to transport vesicles and selectively interact with proteins anchored at appropriate target sites of membrane fusion inspired a simple and compelling explanation of how proteins might be transferred and segregated within the cell, the "S...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-07-01 00:00:00
abstract::Myeloid hematopoietic precursor cells are induced to differentiate by the macrophage and granulocyte differentiation-inducing protein MGI-2 (DF). This differentiation-inducing protein bound to double-stranded but not to single-stranded mammalian DNA. The bound MGI-2 was not eluted by high salt, but was eluted by sodiu...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::A fodrin-like protein purified from porcine thyroid cells and characterized by its properties identical to those of pig brain spectrin (F. Regnouf et al., Eur. J. Biochem. 153, 313-319 (1985)) has been localized by immunofluorescence and electron immunocytochemistry in porcine and rat thyroid. Fodrin-like polypeptides...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::The capacity of vascular endothelial cells to modulate their phenotype in response to changes in environmental conditions is one of the most important characteristics of this cell type. Since different growth factors may play an important signalling role in this adaptive process we have investigated the effect of endo...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00084
更新日期:2000-09-01 00:00:00
abstract::The distribution of microtubules and centrosomes was studied during sheep fertilization by electron and immunofluorescence microscopy. Tubulin and centrosomal material was identified with monoclonal anti-alpha-tubulin and MPM-2 antibodies, respectively. In ovulated eggs, microtubules were exclusively found in the meio...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract::In Physarum polycephalum (Myxomycetes) aphidicolin has been found to delay metaphase onset when applied to synchronous plasmodia 3 h before control metaphase. In contrast to the action of temperature shifts, aphidicolin treatment did not delay the initiation of the increase of thymidine kinase synthesis (EC 2.7.1.21, ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::Hydrogencarbonate and chloride activated, ouabain-insensitive ATPase activities are demonstrated in the salt-absorbing rectum of larval dragonflies. Maximal activation is achieved at approx. 30 mM HCO3- and 20 mM Cl-, respectively. The stimulation of each anion obeys Michaelis-Menten kinetics Km values are 4.65 mM for...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-02-01 00:00:00
abstract::The macronuclear DNA synthesis in well-fed carnivorous forms of Tetrahymena paravorax has been investigated with autoradiographic experiments. The macrostome cell type of this ciliate is induced by stomatin and fed on living prey (T. pyriformis). The DNA synthesis is re-initiated when the macrostomes become active pre...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::Initial integrin-mediated cell-matrix adhesions (focal complexes) appear underneath the lamellipodia, in the regions of the "fast" centripetal flow driven by actin polymerization. Once formed, these adhesions convert the flow behind them into a "slow", myosin II-driven mode. Some focal complexes then turn into elongat...
journal_title:European journal of cell biology
pub_type: 杂志文章,评审
doi:10.1016/j.ejcb.2005.11.001
更新日期:2006-04-01 00:00:00
abstract::We have previously demonstrated the immunofluorescent localization of the tau and HMW MAPS on microtubules in vivo. When cells were treated with the non-ioni detergent triton before fixation, however treated with the non-ionic detergent triton before fixation, however, the immunofluorescent staining of microtubules wi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::The membrane characteristics of amphioxus myotome cells were investigated using the chopped-current clamp method. The extremely thin myotome lamellae had a membrane resistance of 2.2 to 7.7 k omega cm2 and a capacity of 0.4 to 1.3 microF/micron2. The steady state current-voltage relation showed outward rectification. ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::Leydig cells in developing fetal pig testis contained during the fetal regressive phase large accumulations of intermediate filaments. Before and after this period these filaments were arranged in a criss-cross fashion. In the pig as well as in the dog testis these filaments have been characterized as vimentin. Within...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::Perfused Chara cells capable of resuming ATP-dependent cytoplasmic streaming in low free Ca++ solutions have been examined by electron microscopy for myosin-like filaments. Filaments 44 nm in diameter and up to 3 micron in length have been found associated with the endoplasmic reticulum that along with mitochondria, m...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract::It has been known for many years that trypanosomatids require exogenous essential growth factors in order to divide. Two surface domains are involved in starting nutrient endocytosis: the flagellar pocket and the cytostome. Although the flagellar pocket plays a fundamental role in the endocytic process occurring in se...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1078/0171-9335-00112
更新日期:2000-11-01 00:00:00
abstract::Rabbit antisera against homogeneous rat liver thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 1.6.4.5) were prepared and used for immunohistochemical analysis in adult rats. Immunoreactive thioredoxin and thioredoxin reductase were widely distributed in tissues and organs, but va...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::The ESCRT pathway functions at different subcellular membranes to induce their negative curvature, and it has been largely characterized in model eukaryotes belonging to Opisthokonta. But searches of the genomes of many nonopisthokonts belonging to various supergroups indicate that some of them may harbour fewer ESCRT...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2017.11.004
更新日期:2018-01-01 00:00:00
abstract::Channels of the surface-connected, open canalicular system (OCS) of human platelets serve as the pathway for transport of substances into the cells and as conduits for the discharge of alpha granule products secreted during the platelet release reaction. The purpose of the present study was to determine if both functi...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Secretory granules of GH4C1 cells, a rat pituitary tumor cell line, are known to be induced by the treatment of estradiol (E2), insulin, and epidermal growth factor (EGF). We examined changes in the localization of cathepsins B, H, and L, lysosomal cysteine proteinases, in GH4C1 cells before and after hormonal treatme...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Phagosomes with pathogenic mycobacteria, or with hydrophobic polystyrene beads of 1 micron in size, do not mature, but remain fusogenic towards early endosomes and do not fuse with lysosomes (de Chastellier et al. Eur. J. Cell Biol. 68, 167-182 (1995)). Both types of phagocytic particles display a close apposition to ...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::Temperatures around 20 degrees C are known to block degradation of endocytosed material by preventing its transport to lysosomes, accordingly reduced temperature has been widely used to define endosomes. Newer studies have revealed that the low temperature block is proximal to perinuclear late endosomes, but it is not...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/s0171-9335(98)80067-8
更新日期:1998-04-01 00:00:00
abstract::The interaction of daunomycin (DAU), an anthracyclinic antibiotic employed as antitumoral agent, with microtubules, has been investigated by cytochemical and morphological methods on a human melanoma cell line (H14). Results obtained indicated that DAU was able to modulate the microtubule reassembly in cells treated w...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::Extraovarian estrogen production is localized mainly in stromal vascular cells from adipose tissue, which contain the aromatase activity necessary for the conversion of androgens to estrogens. Induction of aromatase activity in these cells by glucocorticoids occurs only in the presence of a serum factor, the propertie...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::Cultured fetal rat hepatocytes derived from 12, 15 and 19-day gestation rats are capable of secreting transferrin. When dexamethasone is added to the medium an increased secretion rate is observed. The changes in secretion rates in control as well as dexamethasone-treated cells during culture have been shown to correl...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
abstract::Initiation of eukaryotic DNA replication is achieved by the sequential binding of different proteins to origins of DNA replication. Using EGFP-tagged initiator proteins and immunofluorescence techniques we found that most of the ORC and the MCM subunits are localised at centrosomes and are colocalised with the polo-li...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2006.09.002
更新日期:2007-01-01 00:00:00
abstract::High-speed centrifugation supernatants from slime mould plasmodia show considerable activities to inhibit the polymerization of actin as revealed by viscosity measurements. By following increasing inhibitory activities an actin modulating protein (AM-protein) has been isolated and purified which affects the polymer st...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
abstract::Since various neuromuscular diseases are associated with abnormal glycosylation, it was of interest to determine whether this key post-translational modification is also altered in aged skeletal muscle. Lectins represent highly versatile carbohydrate-binding proteins that are routinely employed for the characterizatio...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:10.1016/j.ejcb.2008.04.003
更新日期:2008-10-01 00:00:00