Abstract：
:The aim of this study was to characterize further the human skin fibroblast membrane receptor for LDL. We herein report that LDL particles bound to colloidal gold in conjunction with the surface replication technique can be used to visualize steps in the movement of their receptors in the plane of the lipid bilayer and provide the first clear demonstration of the sequential clustering of LDL-receptors into coated pits. Competition experiments have shown that recycled LDL-receptors are inserted in plaques and not at random into unspecialized regions of the plasma membrane. The site of replacement of LDL-receptors into the plasma membrane corresponds to the site of formation of coated pits and their subsequent internalization.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Robenek H,Hesz Asubject
Has Abstractpub_date
1983-09-01 00:00:00pages
275-82issue
2eissn
0171-9335issn
1618-1298journal_volume
31pub_type
杂志文章abstract：:Perfused Chara cells capable of resuming ATP-dependent cytoplasmic streaming in low free Ca++ solutions have been examined by electron microscopy for myosin-like filaments. Filaments 44 nm in diameter and up to 3 micron in length have been found associated with the endoplasmic reticulum that along with mitochondria, m...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-12-01 00:00:00
abstract：:Involvement of CDK2 in glucocorticoid-mediated S-phase lengthening was analyzed in this work. Dexamethasone (DXM) treatment of PHA-stimulated lymphocytes induced a decrease in CDK2 mRNA expression without any change in mRNA stability. This glucocorticoid-induced decrease in CDK2 mRNA expression could be suppressed by ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00298
更新日期：2003-05-01 00:00:00
abstract：:Cell-matrix adhesions are essential for cell migration, tissue organization and differentiation, therefore playing central roles in embryonic development, remodeling and homeostasis of tissues and organs. Matrix adhesion-dependent signals cooperate with other pathways to regulate biological functions such as cell surv...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2008.02.012
更新日期：2008-09-01 00:00:00
abstract：:HeLa cells growing in vitro were treated with the peptidyl aldehyde inhibitor of the chymotrypsin-like activity of the proteasome N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (PSI). Immunofluorescence studies of treated cells revealed the formation of massive perinuclear aggregates rich in ubiquitin and proteas...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:HeLa cells in mitosis are fixed by KMnO4 to obtain a good preservation of membranes. Inside and around the mitotic apparatus a vesicular, a tubular and a cisternal type of membranes are demonstrated. Small spherical or ellipsoidal vesicles are distributed at random in the spindle area. Zones around the poles and in th...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：:Actin and tubulins of Phytophthora infestans germlings were detected with monoclonal antibodies on Western blots of crude extracts separated by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The Mr of actin was approximately 43,000, whereas alpha- and beta-tubulin, which migrated...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:The loss of epidermal growth factor (EGF) binding activity on cultured murine 3T3 cells exposed to EGF (EGF receptor down regulation) was determined in colchicine treated cells, cytochalasin B treated cells, and untreated cells. Neither colchicine nor cytochalasin B altered the affinity of the receptor for EGF, but co...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:The v-mos oncogene product has the ability to induce differentiation in human monocytic leukemia U937 cells, thereby arresting cell proliferation, and also exhibits transforming activity in mouse NIH3T3 cells. Mutation in the v-mos gene consisting of one or two amino acid substitutions in the putative ATP-binding doma...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Recently, we have described the developmental expression of the small heat shock proteins (sHsps) Hsp27/HspB1 and alphaB-crystallin/HspB5 in different tissues of pigs from almost full-term foetuses to three years old adults (P. Tallot, J. F. Grongnet, J. C. David, Biol. Neonate, 83, 281-288, 2003). The data described ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00337
更新日期：2003-10-01 00:00:00
abstract：:Lysosomal Ca2+ release channel TRPML1 has been suggested to regulate lysosome size by activating calmodulin (CaM). To further understand how TRPML1 and CaM regulate lysosome size, in this study, we report that inhibiting mTORC1 causes enlarged lysosomes, and the recovery of enlarged lysosomes is suppressed by inhibiti...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2019.05.001
更新日期：2019-06-01 00:00:00
abstract：:The intracellular storage of zinc in Malpighian tubules of Drosophila hydei was studied by X-ray microanalysis of freeze-dried cryosections. Mass dense vacuoles in the proximal region of the anterior larval Malpighian tubule cells were found to accumulate zinc, not sodium. The zinc content was enhanced considerably af...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:When resting human blood platelets are stimulated with thrombin, 50 to 60% of the G-actin polymerizes to F-actin within 60 seconds. The increase in F-actin is correlated with a corresponding decrease in the complex of G-actin with T beta 4. Within 5 seconds after stimulation, nucleation sites for pyrene actin polymeri...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:The signal transduction pathways of chondrocyte area expansion and migration under periodic mechanical stress remain a matter of debate. We explore this question by performing cell culture experiments in our self-developed periodic stress field and perfusion culture system. Under periodic mechanical stress, we find th...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.04.005
更新日期：2010-09-01 00:00:00
abstract：:Evidence for the presence of a Cdc2-like protein in Physarum polycephalum has been obtained using a peptide antibody directed against a highly conserved amino acid sequence near the N-terminal end of Cdc2, Cdc28 and Cdc2HS. The antibody detected a 34 kDa cytoplasmic protein, similar in apparent size to Cdc2 in yeast a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:Human enteric α-defensins (HD5 and HD6), major antimicrobial peptides produced by Paneth cells in the intestine, play important roles in intestinal innate immunity. Since their expression is decreased in Crohn's disease (CD), with decreased expression being more pronounced in the presence of NOD2 mutations, it would b...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2014.10.007
更新日期：2015-01-01 00:00:00
abstract：:Centromeres support the assembly of the kinetochore on every chromosome and are therefore essential for the proper segregation of sister chromatids during cell division. Centromere identity is regulated epigenetically through the presence of the histone H3 variant CENP-A. CENP-A regulation and incorporation specifical...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2011.04.016
更新日期：2011-10-01 00:00:00
abstract：:In order to investigate the role of microfilaments in the crawling movements of lymphoid cells, we have analyzed the effects of botulinum C2 toxin and of cytochalasin D (cytoD) on the actin cytoskeleton and on the motility of a BW5147 T-lymphoma-derived cell line. Actin was ADP-ribosylated by C2 toxin in the living ce...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:Actin, myosin, and the actin-associated proteins tropomyosin, alpha-actinin, vinculin, and villin were localized in acinar cells of rat and bovine pancreas, parotid, and prostate glands by means of immunofluorescent staining of both frozen tissue sections and semithin sections of quick-frozen, freeze-dried, and plasti...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:NAC (nascent polypeptide associated complex) is a heterodimer consisting of an α and a β subunit. skNAC (skeletal and heart muscle-specific form of αNAC) is a variant of αNAC, which is induced in muscle differentiation and regeneration. We show here that skNAC expression is regulated by p38 MAPK, which plays a crucial...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2011.10.004
更新日期：2012-02-01 00:00:00
abstract：:Bcl-2 can suppress apoptosis by controlling genes that encode proteins required for programmed cell death and by interference with peroxidative damage. Overexpression of Bcl-2 in NIH3T3 cells can prevent GSNO-induced (S-nitrosoglutathione-induced) apoptosis. The experimental results indicated that activation of NF-kap...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/s0171-9335(04)70014-x
更新日期：2000-02-01 00:00:00
abstract：:The concept of pilot pseudopodia is reconsidered 30 years after its inauguration (Gerisch, G., Hülser, D., Malchow, D., Wick, U., 1975. Cell communication by periodic cyclic-AMP pulses. Phil. Trans. R. Soc. Lond. B 272, 181-192). The original hypothesis stated that protruding pseudopodia serve as dynamic sensory organ...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2006.05.002
更新日期：2006-09-01 00:00:00
abstract：:The weak bases chloroquine, primaquine, NH4Cl and the ionophore monensin exert similar but not identical effects on sorting, transport and processing of cathepsin D in several human cell lines (fibroblasts, HepG2 cells, U937, monocytes). The drugs inhibit the segregation of newly synthesized cathepsin D from the secre...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:Differences in the carbohydrate composition were found in the jellies and the cortex of the unfertilized Xenopus laevis egg using lectins and blood group antibodies. Blood group H trisaccharide was detected in the outer jelly and "A-like" oligosaccharide was found in the inner jelly. The blood group A trisaccharide wa...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:We have analyzed the mode of uptake of human beta FcRII molecules expressed in BHK cells (clone 2/14). When challenged with aggregated human IgG (ahIgG), these cells bind the ligand at 4 degrees C and endocytose the IgG: receptor complexes rapidly upon warming to 37 degrees C, as seen by fluorescence microscopy with a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:The recently established in vitro assay of condensation-sorting of pancreatic enzymes to the zymogen granule membrane (ZGM) (Dartsch, H., R. Kleene, H. F. Kern: In vitro condensation-sorting of enzyme proteins isolated from rat pancreatic acinar cells. Eur. J. Cell Biol. 75, 211-222 (1998)) was used to study the invol...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(99)80009-0
更新日期：1999-02-01 00:00:00
abstract：:The role of plasma membrane lipids in regulating the passage of ions and other solutes through the paracellular pathway remains controversial. In this study we explore the contribution of cholesterol (CH) in maintaining the barrier function of an epithelial cell line using the CH-solubilizing agent methyl beta-cyclode...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/s0171-9335(99)80074-0
更新日期：1999-07-01 00:00:00
abstract：:Human peripheral blood monocytes were obtained in very pure preparations by using buoyant density gradient centrifugation followed by velocity sedimentation. We introduced several modifications in the experimental procedure in order to take advantage of the minimal differences in cell density and size between monocyte...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:The evolutionary conserved transmembrane protein Crumbs (Crb) regulates morphogenesis of photoreceptor cells in the compound eye of Drosophila and prevents light-dependent retinal degeneration. Here we examine the role of Crb in the ocelli, the simple eyes of Drosophila. We show that Crb is expressed in ocellar photor...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2012.03.006
更新日期：2012-09-01 00:00:00
abstract：:Invadopodia are actin-rich, adhesive protrusions that extend into and remodel the extracellular matrix. They are associated with high levels of pericellular proteolysis and correlate with the invasive capacity of a variety of tumour cells. Invadopodia have, thus, been proposed to recapitulate key events of the metasta...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2010.04.007
更新日期：2011-02-01 00:00:00
abstract：:In the current study we describe the changes of overall organization of lens fiber cells in connexin 46 (Cx46) and connexin 50 (Cx50) knockout mice. Morphometric analyses and the application of immunocytochemical techniques revealed that in Cx46 knockout lens (Cx46 -/-), where Cx50 is expressed alone, the postnatal di...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2006.03.006
更新日期：2006-08-01 00:00:00
