Abstract：
:Cell-matrix adhesions are essential for cell migration, tissue organization and differentiation, therefore playing central roles in embryonic development, remodeling and homeostasis of tissues and organs. Matrix adhesion-dependent signals cooperate with other pathways to regulate biological functions such as cell survival, cell proliferation, wound healing, and tumorigenesis. Cell migration and invasion are integrated processes requiring the continuous, coordinated assembly and disassembly of integrin-mediated adhesions. An understanding of how integrins regulate cell migration and invasiveness through the dynamic regulation of adhesions is fundamental to both physiological and pathological situations. A variety of cell-matrix adhesions has been identified, namely, focal complexes, focal adhesions, fibrillar adhesions, podosomes, and invadopodia (podosome-type adhesions). These adhesion sites contain integrin clusters able to develop specialized structures, which are different in their architecture and dynamics although they share almost the same proteins. Here we compare recent advances and developments in the elucidation of the organization and dynamics of focal adhesions and podosome-type adhesions, in order to understand how such subcellular sites - though closely related in their composition - can be structurally and functionally different. The underlying question is how their respective physiological or pathological roles are related to their distinct organization.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Block MR,Badowski C,Millon-Fremillon A,Bouvard D,Bouin AP,Faurobert E,Gerber-Scokaert D,Planus E,Albiges-Rizo Cdoi
10.1016/j.ejcb.2008.02.012subject
Has Abstractpub_date
2008-09-01 00:00:00pages
491-506issue
8-9eissn
0171-9335issn
1618-1298pii
S0171-9335(08)00052-6journal_volume
87pub_type
杂志文章,评审abstract：:HeLa cells were treated with different concentrations of an inhibitor of the proteasome chymotrypsin-like activity, the peptidyl aldehyde N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (PSI). A detailed analysis, which included flow cytometry, cell counting and morphological assessment, was performed. PSI treatme...
journal_title：European journal of cell biology
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doi：
更新日期：1996-06-01 00:00:00
abstract：:Lipopolysaccharide (LPS) is known to mediate angiogenic effects in endothelial cells. The underlying mechanisms, however, remain largely unknown. In this study, we showed that LPS induced high motility group box protein 1 (HMGB1) secretion in human pulmonary microvascular endothelial cells (HPMECs). Knockdown and over...
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journal_title：European journal of cell biology
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doi：
更新日期：1986-12-01 00:00:00
abstract：:This paper explores the mode of action of the tranquillizers chloral hydrate and diazepam during fertilization and mitosis of the first reproductive cell cycles in sea urchin eggs. Most striking effects of these drugs are the alteration of centrosomal material and the abnormal microtubule configurations during exposur...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80041-1
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abstract：:The Semper cells in the ommatidia of the blowfly contain an impressive cytoskeleton consisting of hundreds of microtubules. The microtubules are intimately interwoven by an extensive system of the smooth endoplasmic reticulum. The cells were permeabilized by saponin treatment, subsequently incubated in a loading mediu...
journal_title：European journal of cell biology
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doi：
更新日期：1983-11-01 00:00:00
abstract：:The nuclear lamins are karyophilic proteins located at the nucleoplasmic surface of the inner nuclear membrane. We have constructed mutants immediately N-terminal to the nuclear localization signal of human lamin A to identify sites regulating the nuclear transport of the protein. Using an in vitro transport assay, we...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:We have measured the rate of secretion in vitro of individual rat exocrine pancreatic enzymes and proenzymes from cells in pancreatic lobules of control animals and animals subjected to 24 h optimal hormonal prestimulation with cerulein, in vivo. With the controls secreted proteins were first detectable at 20 to 25 mi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:In this study, we investigated the effect of plant microtubule-associated proteins (MAPs) on microtubule nucleation and growth in vitro. Since it has recently been demonstrated that plant nuclear surface acts as a microtubule-organizing center (MTOC), we tested the effects of plant MAPs using a nucleus-mediated microt...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Translin is an evolutionarily conserved approximately 27-kDa protein that binds to specific DNA and RNA sequences and has diverse cellular functions. Here, we report the cloning and characterization of the translin orthologue from the fruit fly Drosophila melanogaster. Under protein-denaturing conditions, purified Dro...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2006.11.005
更新日期：2007-03-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:The linkage of the different types of cytoskeletal proteins to cell adhesion structures at the cytoplasmic membrane and the connection of these contact sites to corresponding sites of adjacent cells is a prerequisite for integrity and stability of cells and tissues. The structurally most prominent types of such cell-c...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2004.12.020
更新日期：2005-03-01 00:00:00
abstract：:Rabbit antisera against homogeneous rat liver thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 1.6.4.5) were prepared and used for immunohistochemical analysis in adult rats. Immunoreactive thioredoxin and thioredoxin reductase were widely distributed in tissues and organs, but va...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:We have reported previously that the human promyelocytic leukemia cell line HL-60, in its undifferentiated state, is devoid of cytoplasmic intermediate filament proteins and nuclear lamins A and C, but does express lamin B. Using immunofluorescence and immunoblotting techniques, we have further investigated the expres...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:The yeast dynamin Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains u...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2017.02.004
更新日期：2017-03-01 00:00:00
abstract：:Filamentous networks were isolated from rat liver and studied by electron microscopy after negative staining with uranyl acetate. Highly purified preparations of networks were used to obtain material for immunological studies, but morphological studies were made more satisfactorily on preparations purified only by suc...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:Morphological explanations for the effects of cytochalasin B (CB) on the digestive vacuole system of Paramecium caudatum were sought using electron-microscopic studies. Cytochalasin B (0.3 mM) essentially stopped vacuole release without stopping vacuole growth. Acidosome fusion with the new vacuoles was inhibited and ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:In the literature the question of whether a system structurally and functionally related to the barrier function of the tight junctions (TJs) of polarized epithelia exists in the epidermis has been and still is controversially discussed. We have systematically addressed this question in a study of the granular layer o...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00434
更新日期：2004-12-01 00:00:00
abstract：:The simultaneous addition of alcian blue and tannic acid to human blood platelets during glutaraldehyde fixation leads to an excellent representation of coated membranes (CM). Coated membrane segments can be found (1) in plasmalemma, in vesicle system and lysosomal granules, or (2) as coated pits or coated vesicles. T...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:The major families of proteoglycans in human arterial tissue have been localized and characterized by electron microscopy. After staining with the polycationic dye cuprolinic blue in the presence of a critical electrolyte concentration, three differently sized populations of proteoglycan-cuprolinic blue precipitates a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
abstract：:Dendritic cells (DC) are the most effective cells for antigen presentation in primary immune responses. Human cord blood CD34+ progenitors cultured in the presence of GM-CSF and TNF alpha generate a heterogeneous population of DC including Langerhans-like DC (LLDC) and monocytes. We describe here that IL-4 exerts diff...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80124-6
更新日期：1998-03-01 00:00:00
abstract：:Human cytomegalovirus (HCMV) infection causes a rapid, progressive disruption of the host cell cytoskeleton that correlates with actin depolymerization. Whole-mount (3D) electron microscopy was used to analyze the cytoskeleton of uninfected and HCMV-infected human lung fibroblast cells. Within 2 min of HCMV infection,...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/S0171-9335(98)80112-X
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abstract：:Specific rabbit antisera were prepared against purified aggregation factor and its membrane-associated receptor, baseplate, derived from the marine sponge. Microciona prolifera. They were utilized in conjunction with fluorescent-labeled goat anti-rabbit IgG in an assay to demonstrate the surface localizations of both ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:Neurofilament (NF) structure and ability to form cross-bridges were examined by quick-freeze deep-etch mica and low-angle rotary-shadow electron microscopy in NFs purified from bovine spinal cord and reassembled in various combinations of NF subunits. When NFs were reassembled from triplet proteins, NF-L, NF-M and NF-...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:Coexistence of four different forms of chromatin was observed by electron microscopy in nuclear spread preparations of monkey kidney cells during late stages of infection with herpes simplex virus (HSV-1 AMG). Besides typical nucleosomal (i) chromatin, thin (3-5 nm) strands morphologically indistinguishable from prote...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:The absence of nucleosome-like structures from purified nuclei of the primitive dinoflagellate Prorocentrum micans was demonstrated by three means. i) Electron microscopy revealed mostly thin, smooth 6-nm nucleofilaments in chromatin incubated at various ionic strengths and either fixed in 0.1% glutaraldehyde or unfix...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:The transition in mouse teratocarcinomas of pluripotential stem cells to histogenetically determined ones of the neurogenic cell lineage was analysed at the total cellular protein level by two-dimensional gel electrophoresis. The change in morphology and function was found to be reflected by extensive shifts in the pr...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Invadopodia are actin-rich, adhesive protrusions that extend into and remodel the extracellular matrix. They are associated with high levels of pericellular proteolysis and correlate with the invasive capacity of a variety of tumour cells. Invadopodia have, thus, been proposed to recapitulate key events of the metasta...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
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更新日期：2011-02-01 00:00:00
abstract：:Initiation of eukaryotic DNA replication is achieved by the sequential binding of different proteins to origins of DNA replication. Using EGFP-tagged initiator proteins and immunofluorescence techniques we found that most of the ORC and the MCM subunits are localised at centrosomes and are colocalised with the polo-li...
journal_title：European journal of cell biology
pub_type： 杂志文章
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