Abstract:
:HeLa cells were treated with different concentrations of an inhibitor of the proteasome chymotrypsin-like activity, the peptidyl aldehyde N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (PSI). A detailed analysis, which included flow cytometry, cell counting and morphological assessment, was performed. PSI treatment induces a significant reduction of mitotic activity, accompanied by metaphase arrest of the mitotic cells. DNA flow cytometry shows an accumulation of the cells in G2+M phases of the cell cycle, which indicates the existence of a proteasome-mediated step in the G2-phase of the cell cycle. After removal of the inhibitor and supplementation with fresh medium, the cell cycle is resumed, but the mitotic cells show increased misalignment of chromosomes in the metaphase plate. PSI also induces HeLa cells to acquire a fibroblastoid phenotype.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Wójcik C,Schroeter D,Stoehr M,Wilk S,Paweletz Nsubject
Has Abstractpub_date
1996-06-01 00:00:00pages
172-8issue
2eissn
0171-9335issn
1618-1298journal_volume
70pub_type
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