Abstract：
:The isolation and characterization of highly purified and structurally well-preserved peroxisomes from the renal cortex of different mammalian species (beef, sheep, and cat) is reported. Renal cortex tissue was homogenized and a peroxisome-enriched light mitochondrial fraction was prepared by differential centrifugation. This was subfractionated by density-dependent banding on a linear gradient of metrizamide (1.12-1.26 g/cm3) using a Beckman VTi 50 vertical rotor. Peroxisomes banded at a mean density of 1.225 cm3. Ultrastructural morphometric examination revealed that peroxisomes made up 97 to 98% of the isolated fractions. By biochemical analysis the contamination with marker enzymes of mitochondria and lysosomes was extremely low. The specific activity of catalase was enriched, depending on the species, between 28- and 38-fold over the homogenate. Peroxisome preparations from all three species exhibited a high but varying level of activity for cyanide-insensitive lipid beta-oxidation. In beef and sheep preparations a small amount of esterase activity cosediments with peroxisomes. These peroxisomes show distinct structural membrane associations with smooth elements of ER. Urate oxidase, a marker enzyme for rat liver peroxisomes, is found only in peroxisomes prepared from beef kidney cortex, with sheep and cat preparations being negative. This correlated with the occurrence of polytubular inclusions in the beef kidney peroxisomes. The large size and the angular shape of isolated peroxisomes as well as the presence of paracrystalline matrical inclusions imply that the majority of peroxisomes are derived from the epithelial cells of the proximal tubule of the kidney cortex. The significant differences found in the characteristics of the renal peroxisomes in three different species investigated, demonstrate the remarkable adaptability and plasticity of this organelle.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Zaar K,Völkl A,Fahimi HDsubject
Has Abstractpub_date
1986-03-01 00:00:00pages
16-24issue
1eissn
0171-9335issn
1618-1298journal_volume
40pub_type
杂志文章abstract：:The monoclonal antibody MPM-12, raised by using partially purified extract of mitotic HeLa cells as the immunogen, preferentially stains the cytoplasm of mitotic cells by indirect immunofluorescence without exhibiting any species specificity. On immunoblots, MPM-12 recognizes three bands, of 155, 88, and 68 kDa, in mi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-02-01 00:00:00
abstract：:The yeast dynamin Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. It has been proposed that Vps1 is functionally linked to clathrin heavy chain 1 (Chc1), but the question of how, where, and when they function together remains u...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2017.02.004
更新日期：2017-03-01 00:00:00
abstract：:Adipogenesis is a complex process strictly regulated by a well-established cascade that has been thoroughly studied in the last two decades. This process is governed by complex regulatory networks that involve the activation/inhibition of multiple functional genes, and is controlled by histone-modifying enzymes. Among...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2014.03.001
更新日期：2014-04-01 00:00:00
abstract：:In Euplotes crassus, the ampules which occur in groups that surround the cilia at their base are organelles which, unlike the extrusomes such as mucocysts and trichocysts, persist inside the cytoplasm and are not lost after discharge. By freeze-fracturing cells, we found membrane specializations denoted by the presenc...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:Glucocorticoid hormones regulate the transcription of nuclear genes by way of their cognate receptors. In addition, these hormones also modulate mitochondrial gene transcription by mechanisms which are as yet poorly understood. Using immunofluorescence labeling and confocal laser scanning microscopy we show that the g...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/S0171-9335(04)70033-3
更新日期：2000-05-01 00:00:00
abstract：:Lamin proteins are components of metazoan cell nuclei. During evolution, two classes of lamin proteins evolved, A- and B-type lamins. B-type lamins are expressed in nearly all cell types and in all developmental stages and are thought to be indispensable for cellular survival. In contrast, A-type lamins have a more re...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2008.06.001
更新日期：2008-11-01 00:00:00
abstract：:Embryonic chick myoblasts possess an extensive network of cytoplasmic microtubules which emanate from a single, perinuclear centrosome containing a microtubule-organizing center (MTOC) and the centrioles. However, after myoblasts fuse into myotubes the centrosome is no longer apparent, and instead long parallel arrays...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:By double indirect immunofluorescence and interference electron microscopy, we have observed the effect of microtubule disruption by antimitotic drugs and coldness treatment on the distribution of adhesion sites and of the three cytoskeletal proteins, vinculin, alpha-actinin, and actin in normal rat cells and in rat c...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:In giant neurons of whip spider legs several filament types are detectable: filaments of 5 to 6 nm thickness as dense masses within the soma of the neuron, an intermediate-sized filament type limited to the dendritic processes forming irregularly wound bundles and finally twisted double filaments in the soma as well a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:Application of carbon tetrachloride produced a progressive proliferation of tight junctions in the rat liver. This system proved to be rapid and highly reproducable and affords the opportunity for tracing the fate of tight junctions in freeze-fracture replicas, facilitating investigations on their formation and functi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:The subcellular distribution of blood group A gene specified alpha 1,3N-acetylgalactosaminyltransferase and its product was studied in human intestinal goblet cells by immunoelectron microscopy. The O-glycosylation step yielding blood group A-active glycoconjugates occurred in the trans region of the Golgi apparatus a...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:Isolated microtubule protein from axopodia of the heliozoan Echinosphaerium nucleofilum, consisting of two major bands on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), has been compared to axonemal and cytoplasmic tubulins from both animal and non-animal sources. The upper E. nucleofilum protein band migrated fas...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:Stereo immunofluorescence microscopy avoids the problem of juxtaposition of structures often encountered in normal fluorescence microscopy. The procedure has been used in conjunction with antibodies against microfilament associated proteins to reveal the arrangement of microfilaments in a rat mammary cell line both in...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:The trafficking of intracellular membranes requires the coordination of membrane-cytoskeletal interactions. Rab proteins are key players in the regulation of vesicular transport, while Rho family members control actin-dependent cell functions. We have previously identified a rho protein, rhoD, which is localized to th...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00173
更新日期：2001-06-01 00:00:00
abstract：:Differences in the carbohydrate composition were found in the jellies and the cortex of the unfertilized Xenopus laevis egg using lectins and blood group antibodies. Blood group H trisaccharide was detected in the outer jelly and "A-like" oligosaccharide was found in the inner jelly. The blood group A trisaccharide wa...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:Gap junctions are known to play a role in the control of cell proliferation, and connexins (Cx) are considered to be tumor suppressors. However, the effects of Cx on cell proliferation are dependent on the Cx which is expressed and on the cell type under consideration. We previously found that restoration of cell-to-c...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00245
更新日期：2002-05-01 00:00:00
abstract：:SGLT1, an isoform of Na+-dependent glucose cotransporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine and the kidney, where it plays a pivotal role in the absorption and reabsorption of sugars, respectively. To search the domain responsible for the apical localization of ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1078/0171-9335-00204
更新日期：2001-12-01 00:00:00
abstract：:Activation of neutrophils is accompanied by the oxidative burst, exocytosis of various granule types (degranulation) and a delay in spontaneous apoptosis. The major source of reactive oxygen species (ROS) in human neutrophils is NADPH oxidase (NOX2), however, other sources of ROS also exist. Although the function of R...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2017.03.003
更新日期：2017-05-01 00:00:00
abstract：:Rho GTPase is a master regulator controlling cytoskeleton in multiple contexts such as cell migration, adhesion and cytokinesis. Of several Rho GTPases in mammals, the best characterized is the Rho subfamily including ubiquitously expressed RhoA and its homologs RhoB and RhoC. Upon binding GTP, Rho exerts its function...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2013.09.002
更新日期：2013-10-01 00:00:00
abstract：:Perfused Chara cells capable of resuming ATP-dependent cytoplasmic streaming in low free Ca++ solutions have been examined by electron microscopy for myosin-like filaments. Filaments 44 nm in diameter and up to 3 micron in length have been found associated with the endoplasmic reticulum that along with mitochondria, m...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1979-12-01 00:00:00
abstract：:In response to mitochondrial damage, mitochondria activate mitochondrial dynamics to maintain normal functions, and an imbalance in mitochondrial dynamics triggers multiple programmed cell death processes. Recent studies have shown that phosphoglycerate mutase 5 (PGAM5) is associated with mitochondrial damage. PGAM5 a...
journal_title：European journal of cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ejcb.2020.151144
更新日期：2020-12-07 00:00:00
abstract：:High-speed centrifugation supernatants from slime mould plasmodia show considerable activities to inhibit the polymerization of actin as revealed by viscosity measurements. By following increasing inhibitory activities an actin modulating protein (AM-protein) has been isolated and purified which affects the polymer st...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:A monoclonal antibody (ID5) raised against the synthetic tetradecapeptide corresponding to the C-terminal region of detyrosinated alpha-tubulin showed an unexpected cross-reactivity with beta-tubulin from pig brain tissue. The specificity and the minimal epitope requirements of ID5 were characterized by competitive en...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/s0171-9335(99)80003-x
更新日期：1999-01-01 00:00:00
abstract：:Carbonic anhydrase (CA) XII, an extracellular enzyme involved in the regulation of the microenvironment acidity and tumor malignant phenotype, was originally identified as a protein overexpressed in some types of cancers, including breast cancer. However, the cellular function and mechanism of CAXII remained unclear. ...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2010.03.004
更新日期：2010-08-01 00:00:00
abstract：:The arrangement of carbohydrate molecules on surfaces of fungal cells may play an important role in nonself recognition of these microorganisms by potential invertebrate hosts. Changes in the ability of various galactose and mannose-specific lectins to bind to surface components on cell walls of the insect pathogen Pa...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:We have previously demonstrated the immunofluorescent localization of the tau and HMW MAPS on microtubules in vivo. When cells were treated with the non-ioni detergent triton before fixation, however treated with the non-ionic detergent triton before fixation, however, the immunofluorescent staining of microtubules wi...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：:The aim of this study was to evaluate the influence of peroxisome proliferator-activated receptor γ (PPARγ) on self-renewal of mouse embryonic stem cells (mESCs) in the presence and absence of leukemia inhibitory factor (LIF). We demonstrated that in the presence of LIF, the activation of PPARγ by Rosiglitazone led to...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：10.1016/j.ejcb.2013.03.003
更新日期：2013-04-01 00:00:00
abstract：:In Drosophila embryos, the dorsal gene, a member of the rel family of transcription factors, is a maternal gene required for dorsal/ventral axis specification and mesoderm and neural migration and differentiation. In this report, the presence and distribution of Xrel-1 protein, a Xenopus homologue of the rel family of...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:This electron microscopic study demonstrates that formation of a functional eukaryotic 40S initiation complex is accompanied by conformational changes which obscure the characteristic structural features of the 40S ribosomal subunits and of the initiation factor eIF-3, the only macromolecular components of the complex...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:We have investigated the effects of bafilomycin A1, a specific inhibitor of vacuolar type H(+)-ATPase, on the secretion and intracellular distribution of prolactin in cultured rat pituitary tumor cells (GH3 cells). Pulse-labeling the cells with L-[35S]methionine for 5 min and subsequently incubating in chase medium co...
journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
