Abstract：
:Gap junctions are known to play a role in the control of cell proliferation, and connexins (Cx) are considered to be tumor suppressors. However, the effects of Cx on cell proliferation are dependent on the Cx which is expressed and on the cell type under consideration. We previously found that restoration of cell-to-cell communication by stable transfection of two independent thyroid-derived cell lines, FRTL-5 and FRT cells, with the Cx32 gene induced a marked reduction of their proliferation rate. This study aimed i) at determining whether Cx43, which is coexpressed with Cx32 by thyroid epithelial cells, exerts the same action as Cx32 on cell proliferation and ii) at identifying alterations of the cell cycle control system that might account for the Cx32-induced proliferation slowdown in thyrocytes. In contrast with previous data on different epithelial cell types, we report that restoration of intercellular communication in FRTL-5 and FRT cells by stable expression of Cx43 did not modify their proliferation properties. Cell cycle analyses revealed that the Cx32-induced proliferation slow-down was related to a lengthening of the G1 phase. The level of expression of two regulatory proteins of the Cip/Kip cyclin-dependent kinase inhibitor family, p27kip1 and p2cip1, was increased in the two cell lines expressing Cx32. In conclusion, Cx32 and Cx43, physiologically coexpressed by thyrocytes, have a differential impact on thyroid cell proliferation in vitro. The cyclin-dependent kinase inhibitors, p27kip1 and p21cip1 might represent cell cycle effectors relaying the down-regulatory effect of Cx32 on the proliferation of thyroid epithelial cells.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Flachon V,Tonoli H,Selmi-Ruby S,Durand C,Rabilloud R,Rousset B,Munari-Silem Ydoi
10.1078/0171-9335-00245subject
Has Abstractpub_date
2002-05-01 00:00:00pages
243-52issue
5eissn
0171-9335issn
1618-1298pii
S0171-9335(04)70227-7journal_volume
81pub_type
杂志文章abstract：:The linkage of the different types of cytoskeletal proteins to cell adhesion structures at the cytoplasmic membrane and the connection of these contact sites to corresponding sites of adjacent cells is a prerequisite for integrity and stability of cells and tissues. The structurally most prominent types of such cell-c...
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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更新日期：1980-06-01 00:00:00
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journal_title：European journal of cell biology
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更新日期：1982-04-01 00:00:00
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journal_title：European journal of cell biology
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doi：10.1078/0171-9335-00128
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
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journal_title：European journal of cell biology
pub_type： 杂志文章
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更新日期：1987-12-01 00:00:00
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journal_title：European journal of cell biology
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doi：10.1016/j.ejcb.2012.03.006
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pub_type： 杂志文章
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
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journal_title：European journal of cell biology
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doi：
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journal_title：European journal of cell biology
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doi：
更新日期：1981-06-01 00:00:00
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
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journal_title：European journal of cell biology
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doi：
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
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journal_title：European journal of cell biology
pub_type： 杂志文章
doi：
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journal_title：European journal of cell biology
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doi：
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journal_title：European journal of cell biology
pub_type： 杂志文章
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更新日期：1997-06-01 00:00:00
