Abstract:
:The amoeba Dictyostelium discoideum produces and secretes multilamellar bodies (MLBs) mainly composed of amoebal membranes upon digestion of bacteria. After their secretion, the fate of these MLBs remains unknown. The aim of this study was to determine if protozoa can internalize and digest secreted D. discoideum MLBs. Our results showed that MLBs were ingested by naive axenic D. discoideum cells (i. e. cells not exposed to bacteria and consequently not producing MLBs). Only a small fraction of the ingested MLBs were found in cells' post-lysosomes compared to undigestible beads suggesting that naive amoebae digest them. D. discoideum MLBs were also ingested by the ciliates Tetrahymena pyriformis and Tetrahymena thermophila. MLBs internalized by the ciliates were compacted into pellets and expelled in the extracellular medium without obvious signs of degradation. The results of this study provide new insights on the biological function of MLBs and, considering that MLBs are also involved in bacteria packaging, suggest additional layers of complexity in microbial interactions.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Denoncourt AM,Durocher AF,Paquet VE,Charette SJdoi
10.1016/j.ejcb.2017.10.002subject
Has Abstractpub_date
2017-12-01 00:00:00pages
767-773issue
8eissn
0171-9335issn
1618-1298pii
S0171-9335(17)30200-5journal_volume
96pub_type
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