Abstract:
:Within eukaryotic cells tubulin generally exists in protein families composed of closely related isoforms generated either by differential gene transcription or by posttranscriptional and posttranslational mechanisms. In this study, immunological approaches were used to examine the contribution of posttranslational modifications to tubulin heterogeneity in a centric diatom, Thalassiosira fluviatilis, and to show the spatial distribution of microtubules in these cells during their interphase. After blotting to nitrocellulose, tubulin in cell-free homogenates of T. fluviatilis was recognized by several general tubulin antibodies including one to the tyrosinated isoform, but not by antibodies to detyrosinated, nontyrosinatable nor acetylated tubulins. Immunofluorescent staining of methanol-fixed cells revealed a net-like reticulum of microtubules originating at or near the cell nucleus. For all antibodies, except one (TU-01), results obtained by immunofluorescent experiments corrobated the analysis of blotted tubulins. Furthermore, microtubules exhibited differential staining patterns corresponding to the intensity of antibody reactivity on blots. Antibody to detyrosinated tubulin, as well as TU-01, yielded a spotty pattern of fluorescence on chloroplasts. Microtubules in T. fluviatilis support normal cell function in the absence of detectable amounts of three common posttranslationally modified tubulins, perhaps due to the rigid silica frustule which maintains shape and to the absence of flagella in interphase cells.
journal_name
Eur J Cell Bioljournal_title
European journal of cell biologyauthors
Machell NH,Fritz L,MacRae THsubject
Has Abstractpub_date
1995-05-01 00:00:00pages
50-6issue
1eissn
0171-9335issn
1618-1298journal_volume
67pub_type
杂志文章abstract::When virgin temperature-sensitive mutant cdc28 cells of the yeast Saccharomyces cerevisiae were incubated at restrictive temperatures, they continued to grow, reaching a maximum of 3.3-fold the original size after 24 h. The protein and RNA levels increased during the first 24 h, then gradually decreased. The cells tha...
journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章,评审
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
doi:
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journal_title:European journal of cell biology
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更新日期:1997-03-01 00:00:00
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journal_title:European journal of cell biology
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更新日期:1996-07-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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journal_title:European journal of cell biology
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