当前位置： SCI文献检索 > EUROPEAN JOURNAL OF CELL BIOLOGY期刊下所有文献 > Melanopsin and rhodopsin mediate UVA-induced immediate pigment darkening: Unravelling the photosensitive system of the skin.

Melanopsin and rhodopsin mediate UVA-induced immediate pigment darkening: Unravelling the photosensitive system of the skin.

Abstract：

:The mammalian skin has a photosensitive system comprised by several opsins, including rhodopsin (OPN2) and melanopsin (OPN4). Recently, our group showed that UVA (4.4 kJ/m2) leads to immediate pigment darkening (IPD) in murine normal and malignant melanocytes. We show the role of OPN2 and OPN4 as UVA sensors: UVA-induced IPD was fully abolished when OPN4 was pharmacologically inhibited by AA9253 or when OPN2 and OPN4 were knocked down by siRNA in both cell lines. Our data, however, demonstrate that phospholipase C/protein kinase C pathway, a classical OPN4 pathway, is not involved in UVA-induced IPD in either cell line. Nonetheless, in both cell types we have shown that: a) intracellular calcium signal is necessary for UVA-induced IPD; b) the involvement of CaMK II, whose inhibition, abolished the UVA-induced IPD; c) the role of CAMK II/NOS/sGC/cGMP pathway in the process since inhibition of either NOS or sGC abolished the UVA-induced IPD. Taken altogether, we show that OPN2 and OPN4 participate in IPD induced by UVA in murine normal and malignant melanocytes through a conserved common pathway. Interestingly, upon knockdown of OPN2 or OPN4, the UVA-driven IPD is completely lost, which suggests that both opsins are required and cooperatively signal in murine both cell lines. The participation of OPN2 and OPN4 system in UVA radiation-induced response, if proven to take place in human skin, may represent an interesting pharmacological target for the treatment of depigmentary disorders and skin-related cancer.

journal_name

Eur J Cell Biol

journal_title

European journal of cell biology

authors

de Assis LVM,Moraes MN,Magalhães-Marques KK,Castrucci AML

doi

10.1016/j.ejcb.2018.01.004

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

150-162

issue

3

eissn

0171-9335

issn

1618-1298

pii

S0171-9335(17)30240-6

journal_volume

97

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF CELL BIOLOGY文献大全
  • Inactivation of p120 catenin in mice disturbs intrahepatic bile duct development and aggravates liver carcinogenesis.

    abstract：:p120 catenin (p120ctn) is required for the stability of classic cadherins at the cell surface and is thought to play a central role in modulating cell-cell adhesion. Cytoplasmic p120ctn promotes cell motility, and probably other activities, by modulating the activities of RhoA, Rac and Cdc42. E-cadherin is expressed i...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2016.10.003

    authors： van Hengel J,Van den Broeke C,Pieters T,Libbrecht L,Hofmann I,van Roy F

    更新日期：2016-12-01 00:00:00

  • Identification of a minus end-specific microtubule-associated protein located at the mitotic poles in cultured mammalian cells.

    abstract：:A mitosis-specific centrosomal component was studied with a human autoantibody, SP-H, which immunostained mitotic poles and interphase nuclei, and a single polypeptide with an apparent molecular mass of 200 to 230 kDa in various lines of cultured cells. Early mitotic PtK1 cells treated with 10 micrograms/ml taxol cont...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Maekawa T,Leslie R,Kuriyama R

    更新日期：1991-04-01 00:00:00

  • Orthobunyaviruses and innate immunity induction: alieNSs vs. PredatoRRs.

    abstract：:The taxonomic group of Orthobunyaviruses is gaining increased attention, as several emerging members are causing devastating illnesses among humans and livestock. These viruses are transmitted to mammals by arthropods (mostly mosquitoes) during the blood meal. The nature of their genomic RNA predisposes orthobunyaviru...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2015.06.001

    authors： Schoen A,Weber F

    更新日期：2015-07-01 00:00:00

  • Morphological analysis of ligand uptake and processing: the role of multivesicular endosomes and CURL in receptor-ligand processing.

    abstract：:The receptor-mediated endocytosis and intracellular processing of transferrin and mannose receptor ligands were investigated in bone marrow-derived macrophages, fibroblasts and reticulocytes. Mannosylated bovine serum albumin (BSA) conjugated to colloidal gold (Au-man-BSA) or colloidal gold-transferrin (AuTf) were use...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Harding C,Levy MA,Stahl P

    更新日期：1985-03-01 00:00:00

  • Excess tyrosine rescues the reduced activity of proliferation and differentiation of cultured recessive yellow melanocytes derived from neonatal mouse epidermis.

    abstract：:The murine recessive yellow (Mc1r(e)) is a loss-of-function mutation in the receptor for alpha-melanocyte-stimulating hormone, melanocortin receptor 1 (Mc1r) and produces yellow coats by inducing pheomelanin synthesis in hair follicular melanocytes. However, it is not known whether the Mc1r(e) mutation affects the pro...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2007.03.007

    authors： Hirobe T,Abe H,Wakamatsu K,Ito S,Kawa Y,Soma Y,Mizoguchi M

    更新日期：2007-06-01 00:00:00

  • Retention of the fetoacinar pancreatic (FAP) protein to the endoplasmic reticulum of tumor cells.

    abstract：:The fetoacinar pancreatic (FAP) protein is a specific component of the human exocrine pancreas associated with the differentiation and proliferation of acinar cells. FAP expression is enhanced in cases of pancreatic exocrine cancer and it is found in relatively high concentrations in pathological pancreatic juices. Ho...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Miralles F,Langa F,Mazo A,Escribano MJ

    更新日期：1993-02-01 00:00:00

  • Podosome-type adhesions and focal adhesions, so alike yet so different.

    abstract：:Cell-matrix adhesions are essential for cell migration, tissue organization and differentiation, therefore playing central roles in embryonic development, remodeling and homeostasis of tissues and organs. Matrix adhesion-dependent signals cooperate with other pathways to regulate biological functions such as cell surv...

    journal_title：European journal of cell biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ejcb.2008.02.012

    authors： Block MR,Badowski C,Millon-Fremillon A,Bouvard D,Bouin AP,Faurobert E,Gerber-Scokaert D,Planus E,Albiges-Rizo C

    更新日期：2008-09-01 00:00:00

  • Filamentous proteophosphoglycan secreted by Leishmania promastigotes forms gel-like three-dimensional networks that obstruct the digestive tract of infected sandfly vectors.

    abstract：:Development of Leishmania parasites in the digestive tract of their sandfly vectors involves several morphological transformations from the intracellular mammalian amastigote via a succession of free and gut wall-attached promastigote stages to the infective metacyclic promastigotes. At the foregut midgut transition o...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/S0171-9335(99)80036-3

    authors： Stierhof YD,Bates PA,Jacobson RL,Rogers ME,Schlein Y,Handman E,Ilg T

    更新日期：1999-10-01 00:00:00

  • Induction of megamitochondria by cuprizone(biscyclohexanone oxaldihydrazone). Evidence for an inhibition of the mitochondrial division process.

    abstract：:1. Feeding weanling mice a diet containing 0.5% (w/w) cuprizone (biscyclohexanone oxaldihydrazone) induced megamitochondria of a different type of that previously described (Suzuki, K., Science 163, 81-82 (1969). 2. As in the normal controls, a single population of mitochondria with a narrow size distribution, was det...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Flatmark T,Kryvi H,Tangerås A

    更新日期：1980-12-01 00:00:00

  • Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors.

    abstract：:Endothelial cell-specific molecules are potential targets for new therapeutic strategies in the control of inflammatory reactions, immune responses and neoangiogenesis. We describe the production and characterization of MEC 14.7, a monoclonal antibody directed to murine endothelial cells recognizing a glycosylated pro...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Garlanda C,Berthier R,Garin J,Stoppacciaro A,Ruco L,Vittet D,Gulino D,Matteucci C,Mantovani A,Vecchi A,Dejana E

    更新日期：1997-08-01 00:00:00

  • Integrin alphavbeta3 binding to human alpha5-laminins facilitates FGF-2- and VEGF-induced proliferation of human ECV304 carcinoma cells.

    abstract：:Human ECV304 cells respond reproducibly by tube formation to complex basement membrane matrices. Laminins are major glycoproteins of basement membranes. We therefore studied the ability of ECV304 cells to attach to defined laminin isoforms and to fibronectin, and identified the involved laminin receptors. The cells bo...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/0171-9335-00297

    authors： Genersch E,Ferletta M,Virtanen I,Haller H,Ekblom P

    更新日期：2003-03-01 00:00:00

  • Rearrangement of the hepatocyte cytoskeleton after toxic damage: involution, dispersal and peripheral accumulation of Mallory body material after drug withdrawal.

    abstract：:Large cytoplasmic aggregates (Mallory bodies) containing randomly arranged 10 to 20 nm thick filaments are characteristic structures of hepatocytes in certain liver diseases, especially alcoholic hepatitis of man and griseofulvin intoxication of mouse. Biochemical and immunological studies have shown that such Mallory...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Denk H,Franke WW

    更新日期：1981-02-01 00:00:00

  • Expression of D-amino acid oxidase in Rhodotorula gracilis under induction conditions: a biochemical and cytochemical study.

    abstract：:D-amino acid oxidase is expressed to a high level in the yeast Rhodotorula gracilis (0.3% of total cell protein) through induction by D-alanine in a defined growth medium. Monospecific polyclonal antibodies against pure enzyme were obtained. Western blot analysis showed that the enzyme is synthesized as the mature pol...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Perotti ME,Pollegioni L,Pilone MS

    更新日期：1991-06-01 00:00:00

  • The effect of ZIMET 3164 and ZIMET 3393 on phagocytosis in Tetrahymena pyriformis GL.

    abstract：:Tetrahymena pyriformis GL is a suitable model to study the influence on phagocytotic activity of two membrane-stabilizing benzimidazole nitrogen mustard derivative, ZIMET 3164 and ZIMET 3393 (cytostasan). Treatment by both compounds causes a gradual inhibition of food vacuole formation dependent on the concentrations ...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Brutkowska M,Fritsch RS,Wollweber L

    更新日期：1982-08-01 00:00:00

  • Phosphorylation of p97(VCP) and p47 in vitro by p34cdc2 kinase.

    abstract：:The hexameric ATPase p97/yeast Cdc48p has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. p97/Cdc48p contains two conserved consensus p34cdc2 kinase phosphorylation sites with...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/S0171-9335(99)80055-7

    authors： Mayr PS,Allan VJ,Woodman PG

    更新日期：1999-04-01 00:00:00

  • Immunochemical and electrophysiological characterization of murine connexin40 and -43 in mouse tissues and transfected human cells.

    abstract：:Human HeLa or SkHep1 cells, defective in intercellular communication through gap junctions, were transfected with coding sequences of murine connexin40 (Cx40) and -43. The transfected cells were restored in gap junctional coupling as shown by 100-fold increased electrical conductance. When studied by the double whole-...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Traub O,Eckert R,Lichtenberg-Fraté H,Elfgang C,Bastide B,Scheidtmann KH,Hülser DF,Willecke K

    更新日期：1994-06-01 00:00:00

  • Intact microtubules are necessary for complete processing, storage and regulated secretion of von Willebrand factor by endothelial cells.

    abstract：:The importance of intact microtubules in the processing, storage and regulated secretion of von Willebrand factor (vWf) from Weibel-Palade bodies in endothelial cells was investigated. Human umbilical vein endothelial cells treated for 1 h with colchicine (10(-6) M) or nocodozole (10(-6) M) lost their organized microt...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Sinha S,Wagner DD

    更新日期：1987-06-01 00:00:00

  • Lysosomal Ca2+ release channel TRPML1 regulates lysosome size by promoting mTORC1 activity.

    abstract：:Lysosomal Ca2+ release channel TRPML1 has been suggested to regulate lysosome size by activating calmodulin (CaM). To further understand how TRPML1 and CaM regulate lysosome size, in this study, we report that inhibiting mTORC1 causes enlarged lysosomes, and the recovery of enlarged lysosomes is suppressed by inhibiti...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2019.05.001

    authors： Yang Y,Xu M,Zhu X,Yao J,Shen B,Dong XP

    更新日期：2019-06-01 00:00:00

  • Differential distribution of cytokeratins after microinjection of anti-cytokeratin monoclonal antibodies.

    abstract：:In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequen...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Boller K,Kemler R,Baribault H,Doetschman T

    更新日期：1987-06-01 00:00:00

  • [Biochemical demonstration of HCO3--und Cl--dependent ATPase activities in the rectum of larval dragonflies and inhibition of rectal chloride uptake by thiocyanate (author's transl)].

    abstract：:Hydrogencarbonate and chloride activated, ouabain-insensitive ATPase activities are demonstrated in the salt-absorbing rectum of larval dragonflies. Maximal activation is achieved at approx. 30 mM HCO3- and 20 mM Cl-, respectively. The stimulation of each anion obeys Michaelis-Menten kinetics Km values are 4.65 mM for...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Komnick H,Schmitz M,Hinssen H

    更新日期：1980-02-01 00:00:00

  • P446L-importin-beta inhibits nuclear envelope assembly by sequestering nuclear envelope assembly factors to the microtubules.

    abstract：:The P446L mutant Drosophila importin-beta (P446L-imp-beta) has been reported to prohibit--in dominant negative fashion--nuclear envelope (NE) assembly. Along elucidating the mode of action of P446L-imp-beta we studied in vitro NE assembly on Sepharose beads. While Drosophila embryo extracts support NE assembly over Se...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/0171-9335-00324

    authors： Tirián L,Timinszky G,Szabad J

    更新日期：2003-07-01 00:00:00

  • Electron microscopic study of eukaryotic 40S initiation complex in protein synthesis.

    abstract：:This electron microscopic study demonstrates that formation of a functional eukaryotic 40S initiation complex is accompanied by conformational changes which obscure the characteristic structural features of the 40S ribosomal subunits and of the initiation factor eIF-3, the only macromolecular components of the complex...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Boublik M,Hellmann W,Staehelin T,Trachsel H

    更新日期：1983-11-01 00:00:00

  • Acetylation of translationally controlled tumor protein promotes its degradation through chaperone-mediated autophagy.

    abstract：:Translationally controlled tumor protein (Tpt1/TCTP) is a multi-functional cytosolic protein whose cellular levels are finely tuned. TCTP regulates protein behavior by favoring stabilization of protein partners or on the contrary by promoting degradation of others. TCTP has been shown to be transcriptionally and trans...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1016/j.ejcb.2016.12.002

    authors： Bonhoure A,Vallentin A,Martin M,Senff-Ribeiro A,Amson R,Telerman A,Vidal M

    更新日期：2017-03-01 00:00:00

  • Characterization of proteins secreted during maize microspore culture: arabinogalactan proteins (AGPs) stimulate embryo development.

    abstract：:To study molecules secreted from cultured plant cells that promote development, maize microspores were transferred into culture and the conditioned media were collected over time and analysed. Electrophoresis indicated that both non-glycosylated and glycosylated proteins including arabinogalactan proteins (AGPs) appea...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：10.1078/0171-9335-00378

    authors： Borderies G,le Béchec M,Rossignol M,Lafitte C,Le Deunff E,Beckert M,Dumas C,Elisabeth MR

    更新日期：2004-07-01 00:00:00

  • Membrane traffic and sorbitol release during osmo- and volume regulation in isolated rat renal inner medullary collecting duct cells.

    abstract：:In response to hypotonic stress, cells of the inner medullary collecting duct (IMCD) undergo swelling followed by a regulatory volume decrease (RVD) and a transient release of organic osmolytes such as sorbitol. In this study, we tested the hypothesis whether membrane recycling is involved in the latter process. There...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Czekay RP,Kinne-Saffran E,Kinne RK

    更新日期：1994-02-01 00:00:00

  • A comparative study of the membrane structure in different types of muscle fibers in the frog.

    abstract：:The muscle membrane of slow and fast fibers in cruralis and iliofibularis muscles and of intermediate fibers in submaxillaris muscle of the frog is studied in freeze-fracture replicas. A comparison of membrane folds, number, size and distribution of caveolae and of intramembrane particles (IMP) is given. In slow muscl...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Verma V

    更新日期：1984-09-01 00:00:00

  • Human high-affinity Fc IgG receptor (Fc gamma RI)-mediated phagocytosis and pinocytosis in COS cells.

    abstract：:The high-affinity receptor (Fc gamma RI) for the constant (Fc) portion of immunoglobulin G (IgG) is one of three Fc IgG receptor classes (Fc gamma Rs) found on mononuclear phagocytes. The functional specialization of each of the Fc gamma R classes is not well understood. Previous studies utilizing anti-Fc gamma R mono...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Socolovsky M,Hockaday AR,Allen JM

    更新日期：1994-06-01 00:00:00

  • Properties and functions of TRPM1 channels in the dendritic tips of retinal ON-bipolar cells.

    abstract：:An increase in light intensity induces a depolarization in retinal ON-bipolar cells via a reduced glutamate release from presynaptic photoreceptor cells. The underlying transduction cascade in the dendritic tips of ON-bipolar cells involves mGluR6 glutamate receptors signaling to TRPM1 proteins that are an indispensab...

    journal_title：European journal of cell biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ejcb.2015.06.005

    authors： Schneider FM,Mohr F,Behrendt M,Oberwinkler J

    更新日期：2015-07-01 00:00:00

  • An actin infrastructure is associated with eukaryotic chromosomes: structural and functional significance.

    abstract：:The presence of actin in eukaryotic nuclei, and, especially, its functional significance has not been well established. We have found that under routine immunocytochemical conditions, no actin can be detected in insect follicle cell nuclei by means of antibody (both mono- and polyclonal) or phalloidin staining. Howeve...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Sauman I,Berry SJ

    更新日期：1994-08-01 00:00:00

  • Distribution of sulfated glycosaminoglycans in the glomerular basement membrane and mesangial matrix.

    abstract：:The relative distribution of heparan sulfate-glycosaminoglycan (HS-GAG) and chondroitin sulfate-glycosaminoglycans (CS-GAG) of the mesangial matrix (MM) and the glomerular basement membrane (GBM), which represent the two glomerular extracellular matrices, was determined by a combination of enzymatic treatments and aut...

    journal_title：European journal of cell biology

    pub_type： 杂志文章

    doi：

    authors： Kanwar YS,Jakubowski ML,Rosenzweig LJ

    更新日期：1983-09-01 00:00:00